• Bulletin of the Korean Chemical Society
• /
• 제30권4호
• /
• pp.913-916
• /
• 2009

Femtosecond vibrational spectroscopy was used to probe the dynamics of CO rebinding to hemoglobin (Hb), denatured by 4.0 M GdnHCl in $D_2O# at 283 K, after photolysis of HbCO. The stretching mode of $^{13}CO$ bound to the denatured $Hb^{13}CO$ showed a single band centered at 1922 $cm^{-1}$, indistinguishable from that of denatured $Mb^{13}CO$. Geminate rebinding of CO to the denatured Hb was accelerated more than 1000 times, suggesting that the native structure of the Hb is required to suppress efficient geminate rebinding of CO, as is the case in Mb. The geminate yield and rate for CO rebinding are almost the same in both the denatured Hb and Mb. Similarity in the equilibrium spectrum and rebinding dynamics of CO indicates that the state of the denatured Hb is very similar to that of the denatured Mb. In the denatured Hb, quaternary contact of the protein is likely severed, with the denatured protein existing as an independent subunit much like Mb.

• 한국생물정보학회:학술대회논문집
• /
• 한국생물정보시스템생물학회 2005년도 BIOINFO 2005
• /
• pp.407-411
• /
• 2005

KUGI (Korean UniGene Information) database contains the annotation information of the cDNA sequences obtained from the disease samples prevalent in Korean. A total of about 157,000 5'-EST high throughput sequences collected from cDNA libraries of stomach, liver, and some cancer tissues or established cell lines from Korean patients were clustered to about 35,000 contigs. From each cluster a representative clone having the longest high quality sequence or the start codon was selected. We stored the sequences of the representative clones and the clustered contigs in the KUGI database together with their information analyzed by running Blast against RefSeq, human mRNA, and UniGene databases from NCBI. We provide a web-based search engine fur the KUGI database using two types of user interfaces: attribute-based search and similarity search of the sequences. For attribute-based search, we use DBMS technology while we use BLAST that supports various similarity search options. The search system allows not only multiple queries, but also various query types. The results are as follows: 1) information of clones and libraries, 2) accession keys, location on genome, gene ontology, and pathways to public databases, 3) links to external programs, and 4) sequence information of contig and 5'-end of clones. We believe that the KUGI database and search system may provide very useful information that can be used in the study for elucidating the causes of the disease that are prevalent in Korean.

• Journal of Microbiology and Biotechnology
• /
• 제25권8호
• /
• pp.1234-1240
• /
• 2015

Heat shock RNA 1 (HSR1) is described as a "eukaryotic heat-sensing noncoding RNA" that regulates heat shock response in human and other eukaryotic cells. Highly conserved HSR1 sequences have been identified from humans, hamsters, Drosophila, Caenorhabditis elegans, and Arabidopsis. In a previous study, however, it was suggested that HSR1 had originated from a bacterial genome. HSR1 showed no detectible nucleotide sequence similarity to any eukaryotic sequences but harbored a protein coding region that showed amino-acid sequence similarity to bacterial voltage-gated chloride channel proteins. The bacterial origin of HSR1 was not convincible because the nucleotide sequence similarity was marginal. In this study, we have found that a genomic contig sequence of Comamonas testosteroni strain JL14 contained a sequence virtually identical to that of HSR1, decisively confirming the bacterial origin of HSR1. Thus, HSR1 is an exogenous RNA, which can ectopically trigger heat shock response in eukaryotes. Therefore, it is no longer appropriate to cite HSR1 as a "eukaryotic functional noncoding RNA."

• 한국생활과학회지
• /
• 제8권1호
• /
• pp.193-210
• /
• 1999

The purpose of this study is to compare the similarity of clothing between Modern and Gothic, and between Post-Modern and Renaissance Period. The results are as followes: The beauty of ideal body was changed from unemphasis of body line to emphasis of the erotic espects of body. The style of clothing was changed from simple and functional in order to give freedom in action, to various and complicated in from, and even uncomfortable in order to emphasize individuality. Decoration on clothing like details, trimmings, and various accessories were not prefered in Gothic and Modem Period, but very popular in Post-Modern and Renaissance Period.

• 충청수학회지
• /
• 제29권4호
• /
• pp.585-598
• /
• 2016

This paper shows that the solutions to the nonlinear perturbed differential system $$y^{\prime}=f(t,y)+{\int_{t_0}^{t}}g(s,y(s),T_1y(s))ds+h(t,y(t),T_2y(t))$$, have bounded properties. To show these properties, we impose conditions on the perturbed part ${\int_{t_0}^{t}}g(s,y(s),T_1y(s))ds,h(t,y(t),T_2y(t))$, and on the fundamental matrix of the unperturbed system y' = f(t, y) using the notion of h-stability.

• Radiation Oncology Journal
• /
• 제35권2호
• /
• pp.101-111
• /
• 2017

The number of imaging data sets has significantly increased during radiation treatment after introducing a diverse range of advanced techniques into the field of radiation oncology. As a consequence, there have been many studies proposing meaningful applications of imaging data set use. These applications commonly require a method to align the data sets at a reference. Deformable image registration (DIR) is a process which satisfies this requirement by locally registering image data sets into a reference image set. DIR identifies the spatial correspondence in order to minimize the differences between two or among multiple sets of images. This article describes clinical applications, validation, and algorithms of DIR techniques. Applications of DIR in radiation treatment include dose accumulation, mathematical modeling, automatic segmentation, and functional imaging. Validation methods discussed are based on anatomical landmarks, physical phantoms, digital phantoms, and per application purpose. DIR algorithms are also briefly reviewed with respect to two algorithmic components: similarity index and deformation models.

• 인터넷정보학회논문지
• /
• 제18권4호
• /
• pp.7-17
• /
• 2017

Despite various types of online social networks having different topological and functional characteristics, the kinds of online social networks considered in social recommendations are highly restricted. The pervasiveness of social networks has brought scholarly attention to expanding the scope of social recommendations into more diverse and less explored types of online social networks. As a preliminary attempt, this study examined the information-sharing patterns of a new type of online social network - unilateral (directed) network - and assessed the feasibility of the network as a useful information source. Specifically, this study mainly focused on the presence of shared interests in unilateral networks, because the shared information is the inevitable condition for utilizing the networks as a feasible source of personalized recommendations. As the results, we discovered that user pairs with direct and distant links shared significantly more similar information than the other non-connected pairs. Individual users' social properties were also significantly correlated with the degree of their information similarity with social connections. We also found the substitutability of online social networks for the top cohorts anonymously chosen by the collaborative filtering algorithm.

• 충청수학회지
• /
• 제30권3호
• /
• pp.291-304
• /
• 2017

This paper shows that the solutions to the perturbed differential system $$y^{\prime}=f(t,y)+{{\displaystyle\smashmargin{2}{\int\nolimits_{t_0}}^{t}}g(s,y(s),T_1y(s))ds+h(t,y(t),T_2y(t))$$, have bounded properties by imposing conditions on the perturbed part ${\int}_{t_0}^{t}g(s,y(s),T_1y(s))ds,h(t,y(t),T_2y(t))$, and on the fundamental matrix of the unperturbed system y' = f(t, y) using the notion of h-stability.

• 한국자원식물학회:학술대회논문집
• /
• 한국자원식물학회 2003년도 춘계 학술발표대회
• /
• pp.124-124
• /
• 2003

In order to study gene expression in a reproductive organ, we constructed a cDNA library of immature flower buds in Korean ginseng and generated expressed sequence tags (ESTs) of 3,360 clones randomly selected. The ESTs could be clustered into 1,844 non-redundant groups. Similarity search of the non-redundant ESTs against public non-redundant databases of both protein and DNA indicated that 1,254 groups show similarity to genes of known function. These ESTs clones were divided into sixteen categories depending upon gene function. The most abundant transcripts were unknown protein (72), chlorophyll a/b-binding protein (48), and stylar glycoprotein. There are no useful informations of gene expression during the development of flower bud in Korean ginseng. These results could help to understand the development of flower bud in Korean ginseng.

• Journal of Microbiology
• /
• 제45권2호
• /
• pp.87-97
• /
• 2007

Medium-chain-length polyhydroxyalkanoates (MCL-PHAs), which have constituents with a typical chain length of $C_{6}-C_{14}$, are polyesters that are synthesized and accumulated in a wide variety of Gram-negative bacteria, mainly pseudomonads. These biopolyesters are promising materials for various applications because they have useful mechanical properties and are biodegradable and biocompatible. The versatile metabolic capacity of some Pseudomonas spp. enables them to synthesize MCL-PHAs that contain various functional substituents; these MCL-PHAs are of great interest because these functional groups can improve the physical properties of the polymers, allowing the creation of tailor-made products. Moreover, some functional substituents can be modified by chemical reactions to obtain more useful groups that can extend the potential applications of MCL-PHAs as environmentally friendly polymers and functional biomaterials for use in biomedical fields. Although MCL-PHAs are water-insoluble, hydrophobic polymers, they can be degraded by microorganisms that produce extracellular MCL-PHA depolymerase. MCL-PHA-degraders are relatively uncommon in natural environments and, to date, only a limited number of MCL-PHA depolymerases have been investigated at the molecular level. All known MCL-PHA depolymerases share a highly significant similarity in amino acid sequences, as well as several enzymatic characteristics. This paper reviews recent advances in our knowledge of MCL-PHAs, with particular emphasis on the findings by our research group.