• Title/Summary/Keyword: Functional levels

Search Result 2,068, Processing Time 0.027 seconds

Enhanced Resistance to Botrytis cinerea Mediated by Transgenic Expression of the Spider Chitinase Gene AvChit in Arabidopsis

  • Hur, Yeon-Jae;Kim, Doh-Hoon
    • International Journal of Industrial Entomology and Biomaterials
    • /
    • v.19 no.2
    • /
    • pp.259-264
    • /
    • 2009
  • The AvChit gene encodes for a chitinase from the spider, Araneus ventricosus. This spider, A. ventricosus, is an abundant species in Korea. Arabidopsis thaliana plants were transformed with the AvChit gene using Agrobacterium tumefaciens. Thirteen transgenic lines expressing the AvChit gene were obtained. Functional expression of the AvChit gene in transgenic Arabidopsis was confirmed by Southern, northern and western blot analysis. The AvChit cDNA was expressed as a 61 kDa polypeptide in baculovirus-infected insect Sf9 cells. AvChit protein extracted from transgenic Arabidopsis exhibited high levels of chitinase activity. Phytopathological tests showed that two transgenic Arabidopsis lines expressing the AvChit gene displayed high levels of resistance to gray mold disease (Botrytis cinerea).

Spread Spectrum Method based Power Line Communication for Plant Monitoring and Control System (전력선 통신을 이용한 plant 감시 제어 시스템)

  • 서민상;성석경;안병규
    • Proceedings of the KIPE Conference
    • /
    • 1997.07a
    • /
    • pp.211-215
    • /
    • 1997
  • Localized communication networks for office automation, security monitoring, environmental management of buildings, computer communications, and other applications enjoy every increasing demand. This paper proposes a direct sequence spread spectrum communication system for use in power line data transmission. Advantages of power distribution circuits include reasonably universal coverage and easy access vis a standard wall plug. Disadvantages include limited communication bandwidth, relatively high noise levels, and varying levels of impedance, noise, and attenuation. Spread spectrum signalling provides immunity to narrow-band signal impairments and multiplexing capability. Our prototype power line communication module supports completely physical and data link layers based on the international standard ISO 10368 for reliable high-speed power line communication system. Moreover it provides useful functions to compose a plant monitoring and control system. All the circuits of the communication module are included in one compact circuit. Thus a functional communication system for the power line plant monitoring and control is implemented.

  • PDF

Is Nitric Oxide Involved in Relaxation of Urinary Bladder\ulcorner

  • Chang, Ki-Churl;Chung, Byung-Ha
    • Biomolecules & Therapeutics
    • /
    • v.3 no.1
    • /
    • pp.58-62
    • /
    • 1995
  • We investigated whether nitric oxide (NO) may serve a role in bladder function by immunohistochemical analysis of the distribution of intrinsic NADPH-diaphorase and functional study of isometric tension recordings via a photo-induced adequate nitric oxide (PIANO) generating system using rat bladder. Results suggest that a small number of NADPH-diaphorase-positive perikarya are present within the bladder wall and within adjacent small ganglia. Furthermore, NADPH-diaphorase-positive nerve fibers were observed in the adventitial and muscular layers, subjacent to the urothelium and perivascular fibers. Rat bladder strips precontracted with 3$\mu$M carbachol were reversibly relaxed upon NO generation by UV irradiation. PIANO-mediated relaxation was sensitive to oxygen free radicals. In addition, tissue cGMP levels were increased by the PIANO generating system and elevated cGMP levels were decreased by pretreatment of guanylate cyclase inhibitor, methylene blue. These results indicate that NO may serve a role in modulating bladder tone in the rat.

  • PDF

Recovery Levels of Clustering Algorithms Using Different Similarity Measures for Functional Data

  • Chae, Seong San;Kim, Chansoo;Warde, William D.
    • Communications for Statistical Applications and Methods
    • /
    • v.11 no.2
    • /
    • pp.369-380
    • /
    • 2004
  • Clustering algorithms with different similarity measures are commonly used to find an optimal clustering or close to original clustering. The recovery level of using Euclidean distance and distances transformed from correlation coefficients is evaluated and compared using Rand's (1971) C statistic. The C values present how the resultant clustering is close to the original clustering. In simulation study, the recovery level is improved by applying the correlation coefficients between objects. Using the data set from Spellman et al. (1998), the recovery levels with different similarity measures are also presented. In general, the recovery level of true clusters was increased by using the correlation coefficients.

Tautomerism of Cytosine on Silver, Gold, and Copper: Raman Spectroscopy and Density Functional Theory Calculation Study

  • Cho, Kwang-Hwi;Joo, Sang-Woo
    • Bulletin of the Korean Chemical Society
    • /
    • v.29 no.1
    • /
    • pp.69-75
    • /
    • 2008
  • Tautomerism of pyrimidine base cytosine has been comparatively examined on nanoparticle and roughened plate surfaces of silver, gold, and copper by surface-enhanced Raman scattering (SERS). The SERS spectrum was found to be different depending on the metals and their substrate conditions suggesting the dissimilar population of various tautomers of cytosine on the surfaces. The ab initio calculations were performed at the levels of B3LYP, HF, and MP2 levels of theory with the LanL2DZ basis set to estimate the energetic stability of the tautomers with the metal complexes as well as the gas phase state. The amino group and N3-coordinated tautomer was predicted to be more favorable for bonding to Au, whereas the hydroxyl and N1-coordinated zwitter ionic form is most stable with Ag and Cu as a bidentate form from the DFT calculation. The binding energy with the Ag atom is calculated to be smaller than those with the Au and Cu atoms in line with the temperature-dependent SERS spectra of cytosine.

Analysis of Spin Exchange Interactions in (C2N2H10)[Fe(HPO3)F3] on the Basis of Electronic Structure Calculations

  • Koo, Hyun-Joo
    • Bulletin of the Korean Chemical Society
    • /
    • v.32 no.2
    • /
    • pp.467-471
    • /
    • 2011
  • Spin exchange interactions of $(C_2N_2H_{10})[Fe(HPO_3)F_3]$ were examined by performing a spin dimer analysis based on extended Huckel tight binding method and a mapping analysis based on first principles density functional theory. Spin exchange interactions occur through the super-superexchange paths $J_1$ and $J_2$ in $(C_2N_2H_{10})[Fe(HPO_3)F_3]$. In the super-superexchange path $J_2$ magnetic orbital interactions between eg-block levels are much stronger than those from $t_{2g}$-block levels. Both electronic structure calculations show that the spin exchange interaction through the super-superexchange path $J_2$ is much stronger than that of $J_1$.

The Development and Play Behaviors of Children in Low-Income Families (저소득층 아동의 발달과 놀이에 대한 연구)

  • Kim, Myoung Soon;Kim, Chang Bok;Lee, Mi Wha
    • Korean Journal of Child Studies
    • /
    • v.23 no.1
    • /
    • pp.87-104
    • /
    • 2002
  • This study investigated developmental levels and explored play behaviors in 194 4- and 5-year-old children from low-income families attending 18 daycare centers in Seoul. The Developmental Test for Korean Kindergartners(Korea Institute Curriculum & Evaluation, 1996) was used to assess developmental levels in seven areas. Play behaviors were observed during free-play in their classrooms. Data were analyzed by two-way ANOVA and $x^2$. Results were that the children from low-income families showed highest scores in motor skill development and the lowest scores in mathematical and scientific development. The children engaged most frequently in group-functional play, followed by onlooker behaviors, group-dramatic, and group-constructive play. Onlooker behaviors were the most frequent activity of the 4-year-olds, and the block corner was the most frequently used area during free-play.

  • PDF

A Study on Energy Levels and Electron States of Organic Light-Emitting Materials (유기 발광체의 에너지 준위 및 전자 상태 연구)

  • Kim, Young-Kwan;Kim, Young-Sik;Seo, Ji-Hoon
    • Journal of the Korean Applied Science and Technology
    • /
    • v.22 no.4
    • /
    • pp.299-305
    • /
    • 2005
  • In this study, we designed color of tunable and high efficient organic materials using the quantum dynamics and the semi-empirical calculation, and applied this results to the fabrication of organic light-emitting diodes. Also we optimized the molecular structure of phosphorescent materials and the energy transfer from a host to a dye which makes organic light-emitting diodes improve. Using quantum dynamics method, the molecular structures of ligand only and the whole metal chelate were optimized, and these energy levels were calculated. From this test results, we could understand the emission mechanism of phosphors with various ligands as well as design the proper ligands reducing the T-T annihilation and the carrier lifetime. We also could design ligands with various colors using this test method.

Vulnerability and Information Security Investment Under Interdependent Risks: A Theoretical Approach

  • Shim, Woo-Hyun
    • Asia pacific journal of information systems
    • /
    • v.21 no.4
    • /
    • pp.27-43
    • /
    • 2011
  • This article explores economic models that show the optimal level of information security investment in the presence of interdependent security risks, Using particular functional forms, the analysis shows that the relationship between the levels of security vulnerability and the levels of optimal security investments is affected by externalities caused by agents' correlated security risks. This article further illustrates that, compared to security investments in the situation of independent security risks, in order to maximize the expected benefits from security investments, an agent should invest a larger fraction of the expected loss from a security breach in the case of negative externalities, while an agent should spend a smaller fraction of the expected loss in the case of negative externalities.

Functional Alterations of Sarcoplasmic Reticulum $Ca^{2+}$ Release Channel in Streptozotocin-induced Diabetic Rat Heart

  • Lee, Eun-Hee;Kim, Won-Tae;Kim, Young-Kee;Kim, Young-Hoon;Kim, Hae-Won
    • Proceedings of the Korean Biophysical Society Conference
    • /
    • 2001.06a
    • /
    • pp.67-67
    • /
    • 2001
  • Our previous studies showed that the relaxation defect of diabetic heart was due to the changes in the expressional levels of SR $Ca^{2+}$-ATPase and PLB. In the diabetic heart contractile abnormalities were also observed, and one of the mechanisms for these changes could include alterations in the expression and/or activity levels of various $Ca^{2+}$ regulatory proteins involving cardiac contraction.(omitted)

  • PDF