• The Korea Journal of Herbology
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• v.33 no.4
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• pp.19-26
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• 2018

Objectives : The aim of this study was to investigate the immunopotentiating activity of combine extract that Silkworm and Cinnamomum cassia. Recently, acute epidemic diseases such as cold and viral respiratory diseases have been emerging. So, interested in immunity enhancement has been increasing, and research on natural products to promote immunity activity has been actively conducted. Methods : To confirm the immunopotentiating activity effect, Silkworm (SW), Cinnamomum cassia (CC), and SWCC combined extracts were treated 14 days at 300 mg/kg/day. The changes of glutamic oxalacetic transaminase (GOT), glutamic pyruvate transaminase (GPT) in serum were analyzed after experiment. The changes in the total spleen cell number were measured. Immune cells in spleen were analyzed using fluorescence activated cell sorter (FACS). also, analyzed the expression of cytokines in spleen. Results : Total number of cells in the spleen and FACS analysis of T lymphocytes activated in the spleen showed that the SWCC combined treated group had much higher frequency of active cells than both single groups. The ratio of CD4+CD8+, CD4+CD69+ and CD4+CD25+ T cells in spleen, SWCC is higher than other groups except Nor in CD4+, CD4+CD69+, CD4+CD25+ T cells. The results of this study suggest that SWCC can help immune function via IL-2, IL-10, IL-12, IFN-${\gamma}$ cytokine production, increased T lymphocytes and splenocyte proliferation. Conclusion : Therefore, these results suggested that the SWCC combined extracts administration increase stronger immunity enhancement than when SW and CC adminstration.

• The Korea Journal of Herbology
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• v.33 no.5
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• pp.105-110
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• 2018

Objectives : We tried to observe the fluorescence anisotropy and intensity of ethidium ion in the intercalating binding interaction between DNA and ethidium ions in the presence of berberine, and then tried to explain the effect of berberine on the intercalating interaction of ethidium ion with DNA. Methods : DNA(calf thymus DNA), berberine and ethidium bromide(EtBr) were purchased from Sigma-Aldrich Co. Proper amount of each compound was dissolved in 20 mM sodium phosphate buffer(pH 7.0) containing 100 mM of NaCl to prepare stock solutions. Collections of the fluorescence anisotropy and intensity data were performed on JASCO FP-8300 spectrofluorometer equipped with a polarizer and a Peltier temperature controller. The excitation of ethidium ion was done at 550 nm and the emission data were collected at 600 nm. For Stern-Volmer plot, the fluorescence data were collected at $18^{\circ}C$ and $30^{\circ}C$. Results : According to the results of this research, the weak competitive binding pattern between ethidium ion and berberine appeared in binding with DNA at low ratio of DNA to ethidium ion. But at high ratio of DNA to ethidium ion, this weak competition disappeared. Instead, berberine might bind to DNA by intercalating way. In other words, berberine could de-intercalate ethidium ion from DNA at low concentration of DNA relative to ethidium ion, but could not at high concentration of DNA relative to ethidium ion. In addition, the mechanism of fluorescence quenching of ethidium ion could also proceed differently, depending on the ratio of the amount of DNA to that of ethidium ion. Conclusions : The effect of berberine on the DNA-ethidium ion intercalating interaction could work differently, depending on the relative ratio of the amount of DNA to that of ethidium ion. This study also showed that fluorescence anisotropy analysis is very useful method to obtain detailed information for investigation of the complex binding interactions. In order to fully understand the mechanism of action of the pharmacological effect by berberine, studies on the effect of berberine on the action of proteins such as various enzymes closely related to berberine-induced medicinal effects should be continued.

• Composites Research
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• v.35 no.6
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• pp.394-401
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• 2022

Two polymer precursors, polyvinylidene chloride-resin (PVDC-resin) and polyvinylidene fluoride (PVDF), are assembled into the microporous carbon by pyrolysis. Microporous carbon is advantageous as an electrode for supercapacitors that store electric charges through ion adsorption/desorption. The pyrolysis also turns the various heteroatoms of two precursors into functional groups, contributing to the additional charge storage. The analysis of the porous structure and function group during carbonization are important to develop the carbon for energy storage. Here, we analyzed the functional groups of two polymer-derived carbons through X-ray photoelectron spectroscopy. The electrochemical properties of the functional groups were explored in various pH electrolytes. The specific capacitance of two carbons in the acidic electrolyte (1 M H₂SO₄) was improved compared to that in the neutral electrolyte (0.5 M Na₂SO₄) due to the faradaic charge/discharge reaction of the quinone functional group. In particular, the carbon electrode derived from PVDC-resin exhibits a lower capacity than the carbon from PVDF due to the small micropores. In the alkaline electrolyte (6 M KOH), the highest specific capacitance and rate capability were obtained among the three electrolytes for both electrodes based on the facile adsorption of the constituent electrolyte ions (K⁺, OH^-).

• Education of Primary School Mathematics
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• v.25 no.1
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• pp.57-79
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• 2022

Current mathematics It is necessary to ensure that ratio and proportion concept is not distorted or broken while being treated as if they were easy to teach and learn in school. Therefore, the purpose of this study is to analyze the activities presented in the textbook. Based on prior work, this study reinterpreted the proportional reasoning task from the proportional perspective of Beckmann and Izsak(2015) to the multiplicative structure of Vergnaud(1996) in four ways. This compared how they interpreted the multiplicative structure and relationships between two measurement spaces of ratio and rate units and proportional expression and proportional distribution units presented in the revised textbooks of 2007, 2009, and 2015 curriculum. First, the study found that the proportional reasoning task presented in the ratio and rate section varied by increasing both the ratio structure type and the proportional reasoning activity during the 2009 curriculum, but simplified the content by decreasing both the percentage structure type and the proportional reasoning activity. In addition, during the 2015 curriculum, the content was simplified by decreasing both the type of multiplicative structure of ratio and rate and the type of proportional reasoning, but both the type of multiplicative structure of percentage and the content varied. Second, the study found that, the proportional reasoning task presented in the proportional expression and proportional distribute sections was similar to the previous one, as both the type of multiplicative structure and the type of proportional reasoning strategy increased during the 2009 curriculum. In addition, during the 2015 curriculum, both the type of multiplicative structure and the activity of proportional reasoning increased, but the proportional distribution were similar to the previous one as there was no significant change in the type of multiplicative structure and proportional reasoning. Therefore, teachers need to make efforts to analyze the multiplicative structure and proportional reasoning strategies of the activities presented in the textbook and reconstruct them according to the concepts to teach them so that students can experience proportional reasoning in various situations.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.25 no.2
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• pp.23-38
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• 2022

This study investigates the characteristics and physical habitat requirements for each Bufo gargarizans life history through a literature survey. After deriving variables for each component of Bufo gargarizans, in order to reduce regional deviations from eight previously studied literature research areas for deriving the criteria for variables, a total of 12 natural habitats of Bufo gargarizanss are selected as spatial ranges by selecting four additional sites such as Umyeonsan Ecological Park in Seoul, Wonheungibangjuk in Cheongju in the central region, Changnyeong Isan Reservoir in the southern region, and Mangwonji in Daegu. This study presents Bufo gargarizans SI, a species endemic to Korea, whose population is rapidly declining due to large-scale housing site development and road development, and develops a Bufo gargarizans HSI model accordingly to improve the function of the damaged Bufo gargarizans habitat and to present an objective basis for site selection of alternative habitat. At the same time, it provides basic data for adaptive management and follow-up monitoring. The three basic habitat requirements of amphibians, the physical habitat requirements of Bufo gargarizans, synthesized with shelter, food, and water, and the characteristics of each life history, are classified into five components by adding space and threats through literature research and expert advice. Variables are proposed by synthesizing and comparing the general characteristics of amphibians, among the previously studied single species of amphibians, the components of HSI of goldfrogs and Bufo gargarizans, and the ecological and physical environmental characteristics of Bufo gargarizans. Afterwards, through consultation with an amphibian expert, a total of 10 variables are finally presented by adjacent forest area(ha), the distance between spawning area and the nearest forest land(m), the soil, the distance from the wetland(m), the forest layered structure, the low grassland space, the permanent wetland area(ha), shoreline slope(%), PH, presence of predators, distance from road(m), presence or absence of obstacles. n order to derive the final criteria for each of the 10 variables, the criteria(alternative) for each variable are presented through geographic information analysis of the site survey area and field surveys of the previously studied literature research area. After a focus group interview(FGI) of 30 people related to the Bufo gargarizans colony in Cheongju, a questionnaire and in-depth interviews with three amphibians experts are conducted to verify and supplement the criteria for each final variable. Based on the finally developed Bufo gargarizans HSI, the Bufo gargarizans habitat model is presented through the SI graph model and the drawing centering on the Bufo gargarizans spawning area

• The Journal of the Korea Contents Association
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• v.21 no.12
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• pp.906-917
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• 2021

This study focused on real estate rental income, which is being interested as a means of preparing for old age in the age of low growth and aging. Rental income is seen to function as a safety net of society at a time when it is necessary to live a difficult old age due to the disconnection of income and the extension of the average life span. Therefore, this study conducted the following study on 1,025 households that own rental real estate nationwide. First, the relationship between the characteristics of the household of the rental real estate owner and the real estate rental income was analyzed, and second, it examined whether there is a difference in rental income between the group that engages in income activities other than rental income and the group that only has rental income without income activities. As a result of the analysis, among the demographic and sociological characteristics, gender and spouse were identified as significant variables in rental income. Among the economic characteristics, income and total debt were found to be significant variables. In the case of income activities, rental income was low, and rental income was high when the total debt was high. However, if interest rates rise and the economic recession is prolonged due to unpredictable causes, the owner may suffer from double-use. In preparation for this, it is necessary to review real estate policy alternatives such as easing the period of real estate holdings.

• Journal of Internet Computing and Services
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• v.22 no.6
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• pp.129-140
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• 2021

The next generation mBcN should be built to cooperate with the wireless network to support hyper-speed and hyper-connectivity. In this paper, we propose a network architecture for the cooperation mBcN and 5G commercial network and architecture of the cooperative security gateway required for the cooperation. The proposed cooperative security gateway is between gNB and UPF to support LBO, SFC, and security. Our analysis shows that the proposed architecture has several advantages. First of all, user equipment connected with the mBcN can be easily connected through the 5G commercial radio network to the mBcN. Second, the military application traffic can be transmitted to mBcN without going through the 5G core network, reducing the end-to-end transmission delay without causing the traffic load on the 5G core network. In addition, the security level of the military application can effectively be maintained because the user equipment can be connected to the cooperative security gateway, and the traffic generated by the user equipment is transmitted to the mBcN without going through the 5G core network. Finally, we demonstrate that LBO, SFC, and security modules are essential functions of the proposed gateway in the 5G test-bed environment.

• Journal of Chest Surgery
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• v.54 no.6
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• pp.480-486
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• 2021

Background: Although various methods are already used to calculate predicted postoperative forced expiratory volume in 1 second (FEV₁) based on preoperative FEV₁ in lung surgery, the predicted postoperative FEV₁ is not always the same as the actual postoperative FEV₁. Observed postoperative FEV₁ values are usually the same or higher than the predicted postoperative FEV₁. To overcome this issue, we investigated the relationship between the number of resected lung segments and the discordance of preoperative and postoperative FEV₁ values. Methods: From September 2014 to May 2020, the data of all patients who underwent anatomical lung resection by video-assisted thoracoscopic surgery (VATS) were gathered and analyzed retrospectively. We investigated the association between the number of resected segments and the differential FEV₁ (a measure of the discrepancy between the predicted and observed postoperative FEV₁) using the t-test and linear regression. Results: Information on 238 patients who underwent VATS anatomical lung resection at Kyung Hee University Hospital at Gangdong and by DH. Kim for benign and malignant disease was collected. After applying the exclusion criteria, 114 patients were included in the final analysis. In the multiple linear regression model, the number of resected segments showed a positive correlation with the differential FEV₁ (Pearson r=0.384, p<0.001). After adjusting for multiple covariates, the differential FEV₁ increased by 0.048 (95% confidence interval, 0.023-0.073) with an increasing number of resected lung segments (R²=0.271, p<0.001). Conclusion: In this study, after pulmonary resection, the number of resected segments showed a positive correlation with the differential FEV₁.

• Journal of Ginseng Research
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• v.46 no.3
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• pp.396-407
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• 2022

Background: Colorectal cancer (CRC) has a high morbidity and mortality worldwide. 20 (S)-ginsenoside Rh2 (G-Rh2) is a natural compound extracted from ginseng, which exhibits anticancer effects in many cancer types. In this study, we demonstrated the effect and underlying molecular mechanism of G-Rh2 in CRC cells in vitro and in vivo. Methods: Cell proliferation, migration, invasion, apoptosis, cell cycle, and western blot assays were performed to evaluate the effect of G-Rh2 on CRC cells. In vitro pull-down assay was used to verify the interaction between G-Rh2 and Axl. Transfection and infection experiments were used to explore the function of Axl in CRC cells. CRC xenograft models were used to further investigate the effect of Axl knockdown and G-Rh2 on tumor growth in vivo. Results: G-Rh2 significantly inhibited proliferation, migration, and invasion, and induced apoptosis and G₀/G₁ phase cell cycle arrest in CRC cell lines. G-Rh2 directly binds to Axl and inhibits the Axl signaling pathway in CRC cells. Knockdown of Axl suppressed the growth, migration and invasion ability of CRC cells in vitro and xenograft tumor growth in vivo, whereas overexpression of Axl promoted the growth, migration, and invasion ability of CRC cells. Moreover, G-Rh2 significantly suppressed CRC xenograft tumor growth by inhibiting Axl signaling with no obvious toxicity to nude mice. Conclusion: Our results indicate that G-Rh2 exerts anticancer activity in vitro and in vivo by suppressing the Axl signaling pathway. G-Rh2 is a promising candidate for CRC prevention and treatment.

• Journal of the Korean Burn Society
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• v.23 no.2
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• pp.54-59
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• 2020

Purpose: A fourth degree burn is a full-thickness burn of the skin usually accompanied by damage to deep structures and commonly results in extensive damage to surrounding tissues which makes the treatment of the wound difficult. Coverage of these wound using free flap is known to effective but not commonly used. The purpose of our study is to review our experience and suggest early application of free flap surgery. Methods: A retrospective review was performed from 2010 to 2019, on a total of 34 fourth degree burn patients undergone free flap surgery as primary treatment in our hospital. We reviewed the location of the injury, etiology, TBSA (%), Presence of osteomyelitis, flap choice, complications, period of injury to surgery and healing. Results: Using free flap as a primary reconstrcuction, the outcome is satisfactory. The treatment period was shortened, and there was less loss of function due to complications. Also the incidence of osteomyelitis and amputation was significantly low. Conclusion: Applying free flap surgery as soon as possible in fourth degree burns is effective, such as reducing complications such as infection, reducing amputation, shortening treatment period, and preventing severe sequelae.