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Studies on nuclear transplantation in mouse embryos II. Developmental potential of nuclei from embryos of different developmental stages (생쥐 수정란의 핵이식에 관한 연구 II. 발달단계별 수정란 핵의 이식후 생존성)

  • Park, Choong-saeng;Choe, Sang-yong;Lee, Hyo-jong;Park, Hee-sung
    • Korean Journal of Veterinary Research
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    • v.30 no.4
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    • pp.355-360
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    • 1990
  • Single nuclei from two-, four- and eight-cell mouse embryos were transplanted into enucleated two-cell embryos by micromanipulation and Sendai virus mediated fusion. The developmental potential of these reconstituted embryos in vitro and in vivo was examined. It was found that the single nuclei which were transplanted to enucleated two-cell embryos were not only able to develop to the blastocyst stage in vitro(two-cell nuclei, 76.5%; four-cell nuclei, 68.4%; eight-cell nuclei, 48.3%), but also able to develop to full term in vivo after transfer to recipient mice(two-cell nuclei, 37.1%; four-cell nuclei, 29.6%; eight-cell nuclei, 16.3%). Although the proportion of live young produced after transfer of nucler of nuclear transplant embryos which received eight-cell nuclei was significantly (p<0.05) reduced, it would be suggested that the overall efficiency in producing identical offspring is greater when eight-cell embryos were selected for nuclear donor than two- or four-cell embryos were selected.

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The Effects of SO2 and NH3 on the N2O Reduction with CO over MMO Catalyst (MMO 촉매와 CO 환원제에 의한 N2O 분해에서 SO2 및 NH3 영향 연구)

  • Chang, Kil Sang;You, Kyung-Chang
    • Applied Chemistry for Engineering
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    • v.20 no.6
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    • pp.653-657
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    • 2009
  • Nitrous oxide is a typical greenhouse gas which is produced from various organic or fossil fuel combustion processes as well as chemicals producing plants. $N_2O$ has a global worming potential of 310 times that of $CO_2$ on per molecule basis, and also acts as an ozone depleting material in the stratosphere. However, its removal is not easy for its chemical stability characteristics. Most SCR processes with several effective reducing agents generally require the operation temperature higher than $450^{\circ}C$, and the catalytic conversion becomes decreased significantly when NOx is present in the stream. Present experiments have been performed to obtain basic design data of actual application concerning the effects of $SO_2$ and $NH_3$ on the interim and long term activities of $N_2O$ reduction with CO over the mixed metal oxide (MMO) catalyst derived from a hydrotalcite-like compound precursor. The MMO catalysts used in the experiments, have shown prominent activities displaying full conversions of $N_2O$ near $200^{\circ}C$ when CO is introduced. The presence of $SO_2$ is considered to show no critical behavior as can be met in the $NH_3$ SCR DeNOx systems and the effect of $NH_3$ is considered to play as mere an impurity to share the active sites of the catalysts.

Derivation of Safety Management Implications through Analysis of Major Elevator Failures (승강기 중대고장 분석을 통한 안전관리 시사점 도출)

  • Kim, Beom-Sang;Park, Poem
    • Journal of the Korea Safety Management & Science
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    • v.22 no.3
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    • pp.23-29
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    • 2020
  • As the duty to report and investigate major elevator failures has expanded due to the total amendment of the Elevator Safety Management Act in 2018, more important information on major elevator failures that have been partially identified has been collected. As of 2019, the number of elevators in Korea exceeded 700,000, making it the eighth-class elevator powerhouse in the world, but there is a trend of increasing casualties due to accidents and breakdowns. An Seung-gang-gi is a term that encompasses an elevator that moves vertically and an escalator that moves horizontally. It is an important means of transportation for most citizens that are encountered almost every day in daily life, and it is also necessary as a means of transportation that enables the construction of skyscrapers. And it seems that its importance will never diminish in the future. Major elevator failures are the main cause of dispatch when accumulating the number of 119 dispatches, and the frequency of occurrence is high. It's a shame. According to Heinrich's Law, 300 minor signs and danger phenomena precede, 29 minor accidents and 1 major accident. Accidents caused by elevators are increasing every year due to the increase in the number of installations, and the damage is threatening the valuable lives and property of users and workers in related fields due to fatal risks such as death and serious injury. Elevator safety management can achieve its purpose only when it is managed with the usual interests, awareness of safety, and full efforts of the users, workers, and the government concerned. This study was analyzed based on 2019 data notified to the Korea Elevator Safety Agency on major breakdowns improved after the revision of the Elevator Safety Management Act in 2018, and a total of 8,256 data were analyzed using the SPSS 21 version, a statistical analysis tool, to analyze the correlation with technical statistics. Proceeded. Through the analysis, it was possible to obtain preventive safety management data to prevent serious elevator safety accidents from occurring, and to derive meaningful implications that related safety management and maintenance can be effectively operated to prevent serious failures. In addition, through this analysis, we expect the development of related industries and legal and institutional improvement.

Reliability of a Questionnaire for Women's Reproductive History (여성의 출산력과 초경 및 폐경 연령에 대한 설문지의 신뢰도)

  • Ko, Kwang-Pil;Park, Sue-Kyung;Kim, Yeon-Ju;Bae, Ji-Suk;Jun, Jae-Kwan;Gwack, Jin;Yoo, Keun-Young
    • Journal of Preventive Medicine and Public Health
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    • v.41 no.3
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    • pp.181-185
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    • 2008
  • Objectives : This study was performed to evaluate the reproducibility of a questionnaire concerned with reproductive history and to ascertain which characteristics of the subjects (age, the visit-revisit intervals, education and chronic disease) are associated with good reliability in the Korean Multi-Center Cancer Cohort (KMCC) study. Materials and Methods: A total of 19,688 participants were enrolled between 1993 and 2004. Among them, we selected 386 participants who were aged 40 or more and who re-visited within 8 years after the first visit. Reliability was measured by the percent agreement according to error range for the continuous variables and the percent agreement and kappa statistics for the categorical variables. Results : The pregnancy histories were reliable (kappa=0.67) and the reasons for being menopausal among the postmenopausal women were also reliable (kappa=0.92). The percent agreement of the breast-feeding history was high (96.1%), although the kappa statistic was low. For the continuous variables, when the error range of one variable was considered to be reliable, the percent agreement of the age at menarche and the age at the first full term pregnancy was good (69.4% and 83.6%), whereas that of the age at menopause was low (51.5%). The factors associated with high reliability were a younger age, the presence of chronic disease and a short visit-revisit time interval. Conclusions : The agreements for parity, the reasons for menopause, and the breastfeeding history in the reproductive history questionnaire used in the KMCC were relatively good. The questionnaire for the menarche age and the menopausal age might have lower reliability due to the difference between Korean age and American age. To obtain reliable information, more attention should be given to the items in questionnaire-based surveys, and especially for surveying old-aged women.

LTPP-SPS : Evaluation of Structural Capacity on Asphalt Pavement Reinforced with Glass Fiber (LTPP-SPS : 섬유보강 아스팔트 포장의 구조적 성능 평가)

  • Jeon, Sung-Il;Kim, Boo-Il;Kim, Jo-Sun;Lim, Kwang-Soo
    • International Journal of Highway Engineering
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    • v.10 no.4
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    • pp.281-292
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    • 2008
  • In Korea-LTPP(Long Tenn Pavement Performance) project, the full depth asphalt pavement test sections are constructed on the national highway to evaluate the structural capacity of asphalt pavement reinforced with glass fiber. Truck loading test and FWD test were performed to measure the structural capacity of test sections. Test results showed that the reinforcement of glass fiber installed at between surface and intermediate asphalt layer decreased the strain at the bottom of surface layer and moved up the stress neutral axis in asphalt layer. As a result, the tensile stress was developed at the bottom of intermediate asphalt layer of reinforced asphalt pavement, while the compressive stress was developed at the bottom of intermediate asphalt layer of unreinforced asphalt pavement. On the other hand, the tensile strain at the bottom of asphalt base layer didn't show a difference between glass fiber reinforced and unreinforced pavements. From the FWD test, it was shown that the surface deflection of asphalt pavement reinforced with glass fiber decreased 24 percents comparing to that of unreinforced asphalt pavement. This shows that the reinforcement with glass fiber appears to improve the rutting resistance of asphalt pavement.

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Effects of Sewage Treatment on Characteristics of Sludge as a Composting Material (하수처리가 퇴비화를 위한 하수 슬러지 특성에 미치는 영향)

  • Kim, Jae-Koo;Kim, Jong-Soo
    • Applied Biological Chemistry
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    • v.41 no.2
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    • pp.181-186
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    • 1998
  • The effects of sewage treatment on characteristics of sludge as a composting material were investigated for a year during the initial operation at the full-scale Chunan sewage treatment plant. Due to the shortage of design capacity of belt press, a sludge dewatering unit, non-volatile solids were recirculating and concentrating in the treatment plant, resulting in an increase of MLSS and a decrease in F/M ratio at the activated sludge system. Special attention is required for long term operations since the increase of non-volatile solids in the plant would deteriorates the treatment efficiency. The sewage sludge of the Chunan sewage treatment plant showed 79.5% of water content, 11.6% of organic content, and C/N ratio of 6.1, and contained As 1.8 mg/kg, Cd 27 mg/kg, Hg <0.1 mg/kg, Pb 54 mg/kg, T-Cr 370 mg/kg, and Cu 1,100mg/kg of heavy metals. In order to be used as raw material for optimum composting, the sewage sludge requires bulking agents for moistrure/porosity control and a carbon source for adjusting C/N ratio. However, the sewage sludge is not adequate as a soil conditioner after composing due to a high content of heavy metals. If the sewage sludge has to he used as a soil conditioner after composting, it as required to identify and remove tire industrial wastewater portions in tire influent of the plant since heavy metals in the influent were mostly concentrated in dewatered sludge.

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Conception Types of Elementary School Students about the Moon Phase Changes and the Suggestions and Effects of Teaching Methods (초등학생들의 달의 위상변화에 대한 개념 유형과 수업 방법의 제안 및 효과)

  • Son, Jun Ho
    • Journal of The Korean Association For Science Education
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    • v.35 no.2
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    • pp.289-301
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    • 2015
  • This study noted that elementary school students were unable to accurately comprehend the principles of moon phase changes and that teachers themselves lacked a full understanding of it as well. Therefore, this study classifies conception types through 161 5th grade respondents and suggests how to change students' conception types through the use of reconstructed teaching and learning materials (that have been developed in existing studies). It verified the changes in the learning achievement of 129 5th grade respondents and analyzed how to think about reconstructed teaching and learning materials through four teacher respondents and four 5th grade respondents from the same study. The results of this study are as follows: First, the conception types on moon phase changes were classified into C and W types. W types consisted of W1, W2, W3, W4, and W5 types. Students had difficulty in understanding the principles of a waxing crescent moon and first quarter phase changes. Second, the group taking classes, which implemented reconstructed teaching and learning materials, showed greater improvement in learning achievement posttest and long-term tests compared to those who have not. Finally, teachers and students reacted positively to the reconstructed teaching and learning materials as shown in exit survey results. In conclusion, it is suggested that teachers are better off utilizing reconstructed teaching and learning materials so that elementary school students may fully understand the principles of moon phase changes rather than just memorizing the results.

A Study on the Trend of the International Media's Reports on the EXPO 2012 Yeosu Korea: A Semantic Network Analysis (2012여수세계박람회에 대한 해외언론의 보도추이 분석: 언어 네트워크 분석기법을 중심으로)

  • Kim, Young-Khee;Lee, Jeong-Rock
    • Journal of the Economic Geographical Society of Korea
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    • v.17 no.4
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    • pp.743-758
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    • 2014
  • It has widely been recognized that the EXPO 2012 Yeosu Korea was a succeeded mega event, according to, at least, the international media's attention and reports. This study analysed and compared the trends of the international media's reports on Yeosu in terms of before, preparing, during, and, after periods of the event, through a semantic network analysis. It was revealed that the images of Yeosu have dramatically been upgraded. The city of Yeosu, before the event, was a small port city of South Korea's southern part of peninsula. The city, after the nomination for the next host city of the exposition, was described to a city who had a full potentiality to host a world exposition, not a southern port city of South Korea. After the event was opened, Yeosu was a city of cutting-edge technology and cultural creativity, who had contributed to solve our humankind's pending ecological problems. Even after the events closed, Yeosu was continuously impressed as a ex-city of world exposition, a hub city of Asia trade, and a center for marine ecological restoration. It was suggested that extended monitoring, differentiated communication strategies, long-term planning, and professionalization of the staffs.

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The Relationship between Dental Amalgam Fillings and Urinary Mercury Concentration among Elementary School Children in a Metropolitan Area (대도시지역 일부 초등학생의 치과용 아말감 충전치아와 요중 수은농도의 관련성)

  • Jung, Yun-Sook;Sakong, Joon;An, Seo-Young;Lee, Young-Eun;Song, Keun-Bae;Choi, Youn-Hee
    • Journal of dental hygiene science
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    • v.12 no.3
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    • pp.253-258
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    • 2012
  • Dental amalgam is an alloy composed of a mixture of approximately equal parts of elemental liquid mercury and an alloy powder. Amalgam has been the most popular and effective restorative material used in dentistry. Despite the long history and popularity of dental amalgam as a restorative material, there have been periodic concerns regarding the potential adverse health effects arising from exposure to mercury in amalgam. Since children are more at risk for mercury toxicity, we aimed to assess the association between dental amalgam filling and urinary mercury concentration in children. 581 of elementary school children in grades 1st4th were conveniently recruited from two schools located in Daegu city, Korea. To obtain dental caries experience states, oral examination were conducted using the full term for DFS index, number of amalgam filling surfaces and the type of filling materials. A questionnaire was used to collect information about general characteristics and the frequencies of tooth brushing, gum chewing and fish/seafood consumption. The statistical analysis was done using the SPSS 18.0 program. The mean urinary mercury concentration in children having more surfaces was highest. As a results Urinary mercury concentration of children who have 79 teeth of amalgam filling and more than 10 is higher than without amalgam filling. The number of amalgam filling surface is closely related with urinary mercury concentration.

Oxygen-deficient Reduced TiO2-X: Surface Properties and Photocatalytic Activity

  • Sinhamahapatra, Apurba;Jeon, Jong-Pil;Yu, Jong-Sung
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2016.11a
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    • pp.59-75
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    • 2016
  • Reduced or black $TiO_{2-x}$ materials with oxygen-deficiency have been achieved by creating oxygen vacancies and/or defects at the surface using different methods. Fascinatingly, they exhibited an extended absorption in VIS and IR instead of only UV light with bandgap decrease from 3.2 (anatase) to ~1 eV. However, despite the dramatic enhancement of optical absorption in black $TiO_{2-x}$ materials, they have failed to show expected visible light-assisted water splitting efficiency. This was ascribed to the high concentration of the surface defects and/or oxygen vacancies, considered as an electron donor to enhance donor density and improve the charge transportation in black $TiO_2$ can also act as charge recombination centers, which eventually decrease photocatalytic activity. Therefore, a black ot reducd $TiO_2$ material with optimized properties would be highly desired for visible light photocatalysis. In this report, a new controlled magnesiothermic reduction has been developed to synthesize reduced black $TiO_{2-x}$ in the presence $H_2/Ar$ for photocatalytic $H_2$ production from methanol-water system. The material possesses an optimum band gap and band position, oxygen vacancies, and surface defects and shows significantly improved optical absorption in the visible and infrared region. The synergistic effects enable the reduced $TiO_{2-x}$ material to show an excellent hydrogen production ability along with long-term stability under the full solar wavelength range of light and visible light, respectively, in the methanol-water system in the presence of Pt as a co-catalyst. These values are superior to those of previously reported black $TiO_2$ materials. On the basis of all the results, it can be realized that the outstanding activity and stability of the reduced of $TiO_{2-x}$ NPs suggest that a balanced combination of different factors like $Ti^{3+}$, surface defects, oxygen vacancy, and recombination center is achieved along with optimized bandgap and band position during the preparation employing magnesiothermic reduction in the presence of $H_2$. The controlled magnesiothermic reduction in the presence of $H_2$ is one of the best alternative ways to produce active and stable $TiO_2-based$ photocatalyst for $H_2$ production.

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