• Journal of Veterinary Clinics
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• v.39 no.2
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• pp.51-58
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• 2022

Quercetin, a flavonoid found in fruits and vegetables, exhibits a strong anti-inflammatory activity. The objective of this study was to examine the effect of quercetin on chemotactic activity of peripheral blood polymorphonuclear cells (PMNs) to culture supernatant from peripheral blood mononuclear cells (PBMCs) stimulated with lipopolysaccharide (LPS). In addition, we determined whether this effect is related to interleukin (IL)-8 and changes in cytoskeleton. The chemotactic activity of PMNs was evaluated by a modified Boyden chamber assay. Total cellular filamentous (F)-actin levels were measured by method of fluorescence microscopy. The levels of IL-8 mRNA and protein were measured by real time polymerase reaction method and enzyme-linked immunosorbent assay, respectively. Quercetin (0-50 µM) itself has no chemoattractant effect for PMNs. The culture supernatant from PBMCs (2 × 10⁶ cells/mL) treated with LPS (1 ㎍/mL) showed remarkable increase in chemotaxis of PMNs. However, this effect was reduced dose-dependently by treatment with quercetin. In addition, PBMCs treated with LPS revealed enhanced levels in IL-8 protein and mRNA. Co-treatment of LPS with quercetin (50 µM) in PBMCs decreased IL-8 production and expression. Treatment of quercetin (0-50 µM) on PMNs to rpIL-8 (10 nM) decreased dose-dependently the chemotactic activity of PMNs. Treatment of quercetin on PMNs to IL-8 also reduced their total cellular F-actin level. These results suggested that quercetin attenuates chemotactic activity of PMNs, which is mediated by down-regulation of IL-8 production from LPS-stimulated PBMCs and inhibition of F-actin polymerization in PMNs.

• Korean Journal of Pharmacognosy
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• v.35 no.2 s.137
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• pp.164-170
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• 2004

The flavonoids are a very large and important group of polyphenolic natural products, which are united by their derivatization from the heterocycle, flavone. They are distributed in higher plants and occur widely in the fruits and vegetables that make up the human diet. They exhibit a wide range of biological properties, including antitumor, antiinflammatory, hepatoprotective, antimicrobial, insecticidal and estrogenic activities. They are also major components of many plant drugs and it is possible that they contribute to the curative properties. For the purpose of developing anticancer agent of natural origin, we have evaluated forty four kinds of naturally occurring flavonoids for the inhibitory activity upon the proliferation of cultured human tumor cells such as A549 (non small cell lung), SK-OV-3 (ovary), SK-MEL-2 (melanoma), XF498 (central nerve system) and HCT-15 (colon) in vitro.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.176-176
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• 2017

Hydropriming had positive effects on the time for germination to reach 50%, the germination index, the time to final germination percentage, and the number of uniform seedlings with enlarged cotyledons in in vitro germination of small watermelon. In addition, the highest shoot initiation was obtained from hydroprimed cotyledonary nodes ($95{\pm}6%$), followed by non-primed cotyledonary nodes ($78{\pm}6%$), hydroprimed cotyledons ($72{\pm}4%$), and non-primed cotyledons ($48{\pm}4%$). Meanwhile, no shoots were initiated from hypocotyls. The total number of shoots that initiated from cotyledonary nodes and cotyledon explants was insignificant, indicating that both cotyledons and cotyledonary node were good sources for the in vitro culture. Choosing explant sources that favor tetraploidy should be the key for producing higher polyploidy plants; a total of 10.5% of tetraploid regenerants were entirely identified from cotyledon explants. Cotyledons with highly differentiated cells might show higher variations than cotyledonary nodes with more preexisting meristematic cells. Cells of cotyledon tissue might undergo changes in ploidy level during differentiation of the culture, or it might be that some of the variations were already present in the tissues of the donor plant. Morphological changes in fruit length of tetraploid regenerants are genotype-dependent.

• Microbiology and Biotechnology Letters
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• v.6 no.3
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• pp.115-120
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• 1978

The manufacture of vinegar provides a means of utilizing the juices of many fruits and various kinds of cereals as well as of starchy vegetables. However, no successful attempt has been made to utilize the glutinous rice powder, which is discussed from the Mi Gwa (made of glutinous rice) making, for the manufacture of vinegar. After alcoholic fermentation of the glutinous rice power, it was devided to ferment the liquor to vinegar and to examine factors affecting this fermentation. A laboratory typed vinegar generator was used to convert the alcohol in fermented liquor to acetic acid. Recycling of the fermented liquor through the generator was found essential in this process. Among many strains of Acetobacter cultures used, which was selected from ordinary home made vinegar from all over the Korea, Acetobacter No. Aa-97 proved more efficient for this fermentation. Addition of 1％ phosphate (K$_2$HPO$_4$) and 20％ apple cider vinegar. to the fermented liquor increased both the rate and efficiency of acetic acid fermentation.

• Korean Journal of Environmental Agriculture
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• v.13 no.1
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• pp.66-75
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• 1994

Daily dietary intake of 11 organophosphorus pesticides by the Korean population was estimated to be $31{\mu}g$ per person ($0.567{\mu}g$/kg body weight/day) from known residue data on raw agricultural products and food factor based on the Korean diet, for the period of 1986-90. Intake ratio of 11 pesticides by food group was 56% from cereals, 23% from vegetables, 14% from fruits and 7% from legumes and other food materials. The ratio of the dietary intake to the ADI was 6.1% in diazinon, 5.8% in fenthion, 3.3% in fenitrothion, 1.5% in EPN and 1.7% average for 11 individual pesticides whereas the cumulative ratio of 11 pesticides was 18.7%. It is, therefore, proposed that a systematic estimate for the dietary intake of organophosphorus pesticides should be undertaken, although the present intake level would not give any adverse effect to the health of the Korean population under the current situation of pesticide usage.

• Journal of Korea Society of Industrial Information Systems
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• v.25 no.1
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• pp.55-69
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• 2020

This study examined in detail how the store characteristics and store owner characteristics of nadle store affect the management performance. First, as a result of analyzing the sales results according to the business experience and the same residency, the non-resident of the workplace was 842,000 won, which was 92,000 won higher than the residence. Among the store owner with experience, non-residents were the highest with 918,000 won. Second, as a result of analyzing the sales performance according to the operation type and the store size, the highest sales type was the mart type, followed by the inclusion of fruits and vegetables with an average of 874,000 won. When looking at the size of stores, more than 60㎡ was 790,000 won. The highest operating type of sales over 60㎡ was mart type, which was 960,000 won, followed by convenience stores with an average of 933,000 won. These results will serve as a reference for suggesting not only the small business policy of the government but also the management efficiency of retail stores.

• International Journal of Industrial Entomology and Biomaterials
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• v.37 no.2
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• pp.109-116
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• 2018

We investigated the effects of honey bee (Apis mellifera L.) colony size on the pollination of greenhouse-cultivated watermelon grown under the forcing cultivation system. The highest pollination activity of bees was observed ($14.3{\pm}5.0$ honey bees/day) when the bee colony size was 10,000 followed by 7,500 and 5,000 honey bees. There was a positive correlation between the bee colony size and pollination activity (R = 0.262) but insignificant difference in fruit set with different honey bee colony sizes (88%-91%). Evaluation of physical properties revealed that the weight and shape of watermelon were also not significantly different among different colony sizes. However, larger the bee colony size, higher the number of seeds were fertilized and rate of seed fertilization (p > 0.05). Number of seeds and content of sugar were negatively correlated (R = -0.714). Fertilized seeds showed a significant increase in mealy flesh, which has a negative effect on fruit quality, compared with that of the unfertilized seeds. Overall, we found that a colony size of 5,000 honey bees was the most effective for the pollination of watermelon grown under forcing cultivation. A comparison of the effects of bee pollination with those of artificial pollination suggested that artificial pollination can be effectively replaced by bee pollination in the forcing cultivation of watermelon, because fruit set, weight, and shape by bee pollination were similar to those achieved by artificial pollination.

• Journal of Pharmaceutical Investigation
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• v.33 no.4
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• pp.305-310
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• 2003

One of the possible mechanisms of multi-drug resistance found in cancer cells is the over-expression of P­glycoprotein (P-gp). Studies have shown that compounds in plants including vegetables and fruits not only have anticancer activities but may also modulate P-gp activity. The effect of flavonoids and organic isothiocyanate on P-gp activity was studied in human uterine sarcoma cell lines, MES-SA (sensitive) and MES-SA/DX5 (resistant) cells. The accumulation of daunomycin (DNM), a P-gp substrate, was approximately 10 times greater in the sensitive cell as compared to the resistant cells over the entire time course (up to 2 hours). The positive control, verapamil increased the two hour accumulation of DNM while quercetin decreased that of DNM in the resistant cells. 1-Naphtyl-isothiocyanate (NITC) showed no effect on the two hour accumulation of DNM. The $IC_{50}$ values for DNM in the resistant cells was about 20 times higher than that observed in the sensitive cells $(10.1{\pm}1.7\;{\mu}M\;vs.\;0.58{\pm}0.28\;{\mu}M)$. Verapamil reduced the $IC_{50}$ value for DNM whereas flavonoids (quercetin and fisetin) increased those for DNM in the resistant cells.

• The Korean Journal of Food And Nutrition
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• v.3 no.2
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• pp.161-168
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• 1990

The survey was conducted for the period from 25 th to 29 th July, 1990. The survey area were selected the mountain area in Kang Won Province. Intake of foods and nutrients of all members in the households surveyed by means of questionnaire was computed based on number of meals a day. On the other hand, daily intake of nutrients by an individual was calculated by sex, age and type of work based on the conversion rate of RDA (Korean Recommended Dietary Allowances for Adult). 1. Status of food intake. The average food intake per person per day in surveyed area was 1103.49 g. The total intake of food was consisted of 44.17% grains, 23.31% vegetables, 10.66% fruits, respectively. These findings led us to the conclusion that people in the surveyed area depended heavily on plant foods. 2. Status of nutrient intake, 1) The average intake of Calorie was 2567.54 Cal Per day, which was slightly higher than 2500 Cal of RDA. 2) The average Intake of protein was 82.92g per day, which was higher than 70g of RDA. Though the quantity was above the RDA, it was largely from plant foods 3) The average intake of calcium was 383.93 mg per day, which was much lower than 500 mg of RDA. 4) The average intake of iron was 11.88 me per day, which was nearly the same quantity as 10 mg of RDA. 5) Intake of vitamin group were high among the inhabitants than recommended by RDA 3. The Kinds of food intake The kinds of food intake in surveyed area were totally 66 different kinds. 4. Economic status. As for the education level, almost of the food handlers finished the primary school and the average monthly income was 364,600 in surveyed area. Households used gas(100%) for fuel.

• Korean Journal of Food Science and Technology
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• v.3 no.3
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• pp.135-143
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• 1971

In order to find out the degree of mercury contamination of common foodstuffs a series of determination was carried out by a highly sensitive activation analysis and the following results were obtained. 1. Polished rice contained 0.050 ppm of mercury whereas rice bran had 0.095 ppm mercury which was found in other grain in lesser degree. 2. Vegetables and fruits also contained $0.035{\sim}0.190\;ppm$ of mercury with relatively small variations from sample except persimmon which had a considerably higher amount of mercury. 3. Soybean sprout contained an unexpectantly high amount of mercury. 4. Of the animal products chicken and egg contained more mercury than the meat.