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http://dx.doi.org/10.17555/jvc.2022.39.2.51

Quercetin Reduces Chemotactic Activity of Porcine Peripheral Blood Polymorphonuclear Cells  

Hwa, Gyeong-Rok (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
Ahn, Changhwan (Department of Veterinary Medicine, College of Veterinary Medicine, Jeju National University, Jeju Special Self Governing Province)
Kim, Hakhyun (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
Kang, Byeong-Teck (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
Jeung, Eui-Bae (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
Yang, Mhan-Pyo (Department of Veterinary Medicine, College of Veterinary Medicine, Chungbuk National University)
Publication Information
Journal of Veterinary Clinics / v.39, no.2, 2022 , pp. 51-58 More about this Journal
Abstract
Quercetin, a flavonoid found in fruits and vegetables, exhibits a strong anti-inflammatory activity. The objective of this study was to examine the effect of quercetin on chemotactic activity of peripheral blood polymorphonuclear cells (PMNs) to culture supernatant from peripheral blood mononuclear cells (PBMCs) stimulated with lipopolysaccharide (LPS). In addition, we determined whether this effect is related to interleukin (IL)-8 and changes in cytoskeleton. The chemotactic activity of PMNs was evaluated by a modified Boyden chamber assay. Total cellular filamentous (F)-actin levels were measured by method of fluorescence microscopy. The levels of IL-8 mRNA and protein were measured by real time polymerase reaction method and enzyme-linked immunosorbent assay, respectively. Quercetin (0-50 µM) itself has no chemoattractant effect for PMNs. The culture supernatant from PBMCs (2 × 106 cells/mL) treated with LPS (1 ㎍/mL) showed remarkable increase in chemotaxis of PMNs. However, this effect was reduced dose-dependently by treatment with quercetin. In addition, PBMCs treated with LPS revealed enhanced levels in IL-8 protein and mRNA. Co-treatment of LPS with quercetin (50 µM) in PBMCs decreased IL-8 production and expression. Treatment of quercetin (0-50 µM) on PMNs to rpIL-8 (10 nM) decreased dose-dependently the chemotactic activity of PMNs. Treatment of quercetin on PMNs to IL-8 also reduced their total cellular F-actin level. These results suggested that quercetin attenuates chemotactic activity of PMNs, which is mediated by down-regulation of IL-8 production from LPS-stimulated PBMCs and inhibition of F-actin polymerization in PMNs.
Keywords
quercetin; chemotactic activity; PMNs; IL-8; F-actin;
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1 Beutler B, Rietschel ET. Innate immune sensing and its roots: the story of endotoxin. Nat Rev Immunol 2003; 3: 169-176.   DOI
2 Howard TH, Oresajo CO. The kinetics of chemotactic peptide-induced change in F-actin content, F-actin distribution, and the shape of neutrophils. J Cell Biol 1985; 101: 1078-1085.   DOI
3 Backstrom L, Hoefling DC, Morkoc AC, Cowart RP. Effect of atrophic rhinitis on growth rate in Illinois swine herds. J Am Vet Med Assoc 1985; 187: 712-715.
4 Banks C, Bateman A, Payne R, Johnson P, Sheron N. Chemokine expression in IBD. Mucosal chemokine expression is unselectively increased in both ulcerative colitis and Crohn's disease. J Pathol 2003; 199: 28-35.   DOI
5 Bian Z, Guo Y, Ha B, Zen K, Liu Y. Regulation of the inflammatory response: enhancing neutrophil infiltration under chronic inflammatory conditions. J Immunol 2012; 188: 844-853.   DOI
6 Bourne HR, Weiner O. A chemical compass. Nature 2002; 419: 21.   DOI
7 DesMarais V, Ichetovkin I, Condeelis J, Hitchcock-DeGregori SE. Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge. J Cell Sci 2002; 115(Pt 23): 4649-4660.   DOI
8 Eggesbo JB, Hjermann I, Lund PK, Joo GB, Ovstebo R, Kierulf P. LPS-induced release of IL-1 beta, IL-6, IL-8, TNF-alpha and sCD14 in whole blood and PBMC from persons with high or low levels of HDL-lipoprotein. Cytokine 1994; 6: 521-529.   DOI
9 Grimm MC, Elsbury SK, Pavli P, Doe WF. Interleukin 8: cells of origin in inflammatory bowel disease. Gut 1996; 38: 90-98.   DOI
10 Hwang MK, Song NR, Kang NJ, Lee KW, Lee HJ. Activation of phosphatidylinositol 3-kinase is required for tumor necrosis factor-alpha-induced upregulation of matrix metalloproteinase-9: its direct inhibition by quercetin. Int J Biochem Cell Biol 2009; 41: 1592-1600.   DOI
11 Lee IK, Kye YC, Kim G, Kim HW, Gu MJ, Umboh J, et al. Stress, nutrition, and intestinal immune responses in pigs- a review. Asian-Australas J Anim Sci 2016; 29: 1075-1082.   DOI
12 Liu J, Li X, Yue Y, Li J, He T, He Y. The inhibitory effect of quercetin on IL-6 production by LPS-stimulated neutrophils. Cell Mol Immunol 2005; 2: 455-460.
13 Nair MP, Mahajan S, Reynolds JL, Aalinkeel R, Nair H, Schwartz SA, et al. The flavonoid quercetin inhibits proinflammatory cytokine (tumor necrosis factor alpha) gene expression in normal peripheral blood mononuclear cells via modulation of the NF-kappa beta system. Clin Vaccine Immunol 2006; 13: 319-328.   DOI
14 Osawa Y, Nagaki M, Banno Y, Brenner DA, Asano T, Nozawa Y, et al. Tumor necrosis factor alpha-induced interleukin-8 production via NF-kappaB and phosphatidylinositol 3-kinase/Akt pathways inhibits cell apoptosis in human hepatocytes. Infect Immun 2002; 70: 6294-6301.   DOI
15 Sadhu C, Masinovsky B, Dick K, Sowell CG, Staunton DE. Essential role of phosphoinositide 3-kinase delta in neutrophil directional movement. J Immunol 2003; 170: 2647-2654.   DOI
16 Liu T, Zhang L, Joo D, Sun SC. NF-κB signaling in inflammation. Signal Transduct Target Ther 2017; 2: 17023.   DOI
17 Son SM, Kang JH, Lee GS, Jeung EB, Yang MP. Induction of interleukin-8 expression in porcine peripheral blood mononuclear cells by trans10-cis12 conjugated linoleic acid. Vet Immunol Immunopathol 2006; 112: 284-289.   DOI
18 Sorensen NS, Skovgaard K, Heegaard PM. Porcine blood mononuclear cell cytokine responses to PAMP molecules: comparison of mRNA and protein production. Vet Immunol Immunopathol 2011; 139: 296-302.   DOI
19 Roque K, Shin KM, Jo JH, Lim GD, Song ES, Shin SJ, et al. Association between endotoxin levels in dust from indoor swine housing environments and the immune responses of pigs. J Vet Sci 2018; 19: 331-338.   DOI
20 De Groote D, Zangerle PF, Gevaert Y, Fassotte MF, Beguin Y, Noizat-Pirenne F, et al. Direct stimulation of cytokines (IL-1 beta, TNF-alpha, IL-6, IL-2, IFN-gamma and GM-CSF) in whole blood. I. Comparison with isolated PBMC stimulation. Cytokine 1992; 4: 239-248.   DOI
21 Agarwal S, Piesco NP, Johns LP, Riccelli AE. Differential expression of IL-1 beta, TNF-alpha, IL-6, and IL-8 in human monocytes in response to lipopolysaccharides from different microbes. J Dent Res 1995; 74: 1057-1065.   DOI
22 Baggiolini M, Loetscher P, Moser B. Interleukin-8 and the chemokine family. Int J Immunopharmacol 1995; 17: 103-108.   DOI
23 dos Remedios CG, Chhabra D, Kekic M, Dedova IV, Tsubakihara M, Berry DA, et al. Actin binding proteins: regulation of cytoskeletal microfilaments. Physiol Rev 2003; 83: 433-473.   DOI
24 Bischoff SC. Quercetin: potentials in the prevention and therapy of disease. Curr Opin Clin Nutr Metab Care 2008; 11: 733-740.   DOI
25 Bodin S, Welch MD. Plasma membrane organization is essential for balancing competing pseudopod- and uropod-promoting signals during neutrophil polarization and migration. Mol Biol Cell 2005; 16: 5773-5783.   DOI
26 Cho SY, Park SJ, Kwon MJ, Jeong TS, Bok SH, Choi WY, et al. Quercetin suppresses proinflammatory cytokines production through MAP kinases andNF-kappaB pathway in lipopolysaccharide-stimulated macrophage. Mol Cell Biochem 2003; 243: 153-160.   DOI
27 Eum SY, Kong JH, Hong MS, Lee YJ, Kim JH, Hwang SH, et al. Neutrophils are the predominant infected phagocytic cells in the airways of patients with active pulmonary TB. Chest 2010; 137: 122-128.   DOI
28 Jeon NL, Baskaran H, Dertinger SK, Whitesides GM, Van de Water L, Toner M. Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed in a microfabricated device. Nat Biotechnol 2002; 20: 826-830.   DOI
29 Kukulski F, Ben Yebdri F, Lefebvre J, Warny M, Tessier PA, Sevigny J. Extracellular nucleotides mediate LPS-induced neutrophil migration in vitro and in vivo. J Leukoc Biol 2007; 81: 1269-1275.   DOI
30 Parent CA, Devreotes PN. A cell's sense of direction. Science 1999; 284: 765-770.   DOI
31 Skov L, Beurskens FJ, Zachariae CO, Reitamo S, Teeling J, Satijn D, et al. IL-8 as antibody therapeutic target in inflammatory diseases: reduction of clinical activity in palmoplantar pustulosis. J Immunol 2008; 181: 669-679.   DOI
32 Gao Y, Flori L, Lecardonnel J, Esquerre D, Hu ZL, Teillaud A, et al. Transcriptome analysis of porcine PBMCs after in vitro stimulation by LPS or PMA/ionomycin using an expression array targeting the pig immune response. BMC Genomics 2010; 11: 292.   DOI
33 Souto FO, Zarpelon AC, Staurengo-Ferrari L, Fattori V, Casagrande R, Fonseca MJ, et al. Quercetin reduces neutrophil recruitment induced by CXCL8, LTB4, and fMLP: inhibition of actin polymerization. J Nat Prod 2011; 74: 113-118.   DOI
34 Wang W, Sun C, Mao L, Ma P, Liu F, Yang J, et al. The biological activities, chemical stability, metabolism and delivery systems of quercetin: a review. Trends Food Sci Technol 2016; 56: 21-38.   DOI
35 D'Andrea G. Quercetin: a flavonol with multifaceted therapeutic applications? Fitoterapia 2015; 106: 256-271.   DOI
36 Jo HM, Ahn C, Kim H, Kang BT, Jeung EB, Yang MP. Effect of quercetin on formation of porcine neutrophil extracellular trap. Vet Immunol Immunopathol 2021; 241: 110335.   DOI