• Korean Journal of Agricultural Science
• /
• v.14 no.2
• /
• pp.197-204
• /
• 1987

In order to know the growth of suspended cells by explant sources, the change of nitrogen contents of cultured cells following the growth periods, capability of micro-callus formation according to cell plating methods, growth of suspended cells on various media, and efficiency of micro-callus formation by using growth regulators and different N strengths were investigated. 1. When suspension culture was tried by using the callus induced from internode and petiole, cell fresh weight and packed cell volume increased with similar way and the growth reached at stationary phase after 12 culture days. 2. N-contents of cultured cells increased upto 3 days and decreased around 6days. But the values increased again upto 9 days, after that they showed gradual decreases. 3. Of cell plating methods, embedding method was the best for micro-callus formation. 4. Growth of suspened cells showed the rest performanoes, when they were cultured on LM medium with 1/2N strengths and BAP 0.01.2.4-D 0.1, and NAA $1.0mg/{\ell}$, after 15 cultured days(upto 76.9 folds). LM medium was better than MS or GD. The combination of auxin and cytokinin was better for cell growing than auxin-treatment only. 5. Micro-callus from single cell and small cell aggregates was formed only on MS and LM media with 2,4-D $1.0mg/{\ell}$.

• Horticultural Science & Technology
• /
• v.33 no.4
• /
• pp.585-590
• /
• 2015

This study was conducted to improve the acclimatization rate of in vitro strawberry plantlets through bioreactor culture using the growth retardant flurprimidol. Different concentrations [0 (Control), 0.1, 0.5, 1.0, and $2.0mg{\cdot}L^{-1}$] of flurprimidol were added during bioreactor culture. After six weeks of treatments, various growth characteristics were investigated and in vitro plantlets were acclimated in the greenhouse. The growth rate of treated plantlets was much lower than that of control, and as the treatment concentration increased, the growth rate was much decreased. Shoots of plantlets treated with flurprimidol were shorter (2.2-3.7 cm) than those of control (7.9 cm). The number of roots per treated plant was around 11.6-34.2, compared with 51.8 in the control. Root length was also lower (0.88-3.08 cm) than control (4.36 cm). However, the number of new shoots and leaves increased in all treatments except for $2.0mg{\cdot}L^{-1}$ concentration. The root was partially decayed in $1.0mg{\cdot}L^{-1}$ concentration and was completely decayed in $2.0mg{\cdot}L^{-1}$. The survival rate in $0.1mg{\cdot}L^{-1}$ and $0.5mg{\cdot}L^{-1}$ concentrations was 100% and 23.3% respectively. After four weeks of acclimatization, the plantlets restarted growth, and growth characteristics of shoots and roots recovered to the levels of control, except for fresh weight. Based on our results, a concentration of $0.1mg{\cdot}L^{-1}$ flurprimidol is appropriate for improvement of acclimatization rate of in vitro strawberry plantlets in bioreactor culture.

• Horticultural Science & Technology
• /
• v.30 no.5
• /
• pp.484-492
• /
• 2012

The present study aimed to investigate the effect of static magnetic field (SMF) on the growth and physiological characteristics of common indoor plant species. Five foliage plant species, Spathiphyllum spp., Ardisia pusilla DC., Syngonium podophyllum, Peperomia pereskiifolia, and Pilea cadierei were potted into plastic pot equipped with round type anisotropic sintered NdFeB permanent magnet inside the pot. The surface magnetic flux density of each magnet was 3,500 G. After 6 months of growth period, the biomass accumulations of Spathiphyllum, A. pusilla, and P. cadierei under SMF were statistically higher than those of controls. Tissue water content also increased under the influence of SMF in most species. The photosynthetic rate of Spathiphyllum under SMF significantly increased but other species showed no significant difference compared with control. Although there was no significant increase in the photosynthetic rates of A. pusilla, and P. cadierei, they showed remarkable increase in total fresh weight under SMF. This suggests that the demand of assimilates for normal metabolism could be decreased under magnetic influence and thereby biomass accumulation could be more favored. But this is not always true for all plant species because P. pereskiifolia in this experiment, showed no changes in both photosynthetic rate and biomass accumulation. Leaf nitrogen and chlorophyll contents were enhanced significantly in most plant species under influence of SMF. Chlorophyll a/b ratio also increased by SMF. Although there might be a limitation depending on plant species, these results suggest that long-term exposure to SMF might allow plant to have an enhanced acclimation capacity against environmental fluctuations and optimal application of SMF could increase the practical use of indoor plants such as an attempt to improve indoor air quality.

• Horticultural Science & Technology
• /
• v.35 no.2
• /
• pp.188-198
• /
• 2017

Asparagus (Asparagus officinalis L.) spears were treated with white (color temperature 4,500 k), blue (peak 450 nm), and red (peak 660 nm) light-emitting diodes (LEDs) at a photosynthetic photon flux density (PPFD) of $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ for 12 h, and UV-B (280 nm) at 0.5 kJ or 1.0 kJ to determine the effect on agronomic characteristics, antioxidant phytochemicals, and antioxidant activity. The fresh weight, length, and width of spears were not affected by light quality treatments. The free sugars and chlorophyll contents were increased by 9 and 41%, respectively in the UV-0.5 kJ treatments. Among the antioxidant phytochemicals (vitamin C, total phenol, rutin, and total flavonoid), vitamin C was most greatly affected by the light treatments. Vitamin C content was significantly increased in asparagus spears subjected to the white (114%), red (137%), and UV-0.5 kJ(127%) treatments compared to the control. By contrast, rutin, total phenol, and total flavonoid content were increased only in samples subjected to the red and UV-0.5 kJ treatment. Furthermore, antioxidant activity, as measured by DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity, increased in white, red, and UV-0.5 kJ treatments by about 43, 41, and 43%, respectively, compared to the control. These results suggest that postharvest treatment of asparagus spears with red light at $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ for 12 h or with UV-B (280 nm) at 0.5 kJ could enhance the functional quality of the asparagus spears by increasing the content of phytochemicals like vitamin C, rutin, total phenolics, and total flavonoids.

• Research in Plant Disease
• /
• v.16 no.1
• /
• pp.59-65
• /
• 2010

This study was conducted to select the cultivars and strains which are suitable in cultivation of the Chinese cabbage having good horticultural characteristics and the resistance to clubroot caused by Plasmodiophora brassicae, which is a widespread disease that causes serious problems in many Brassica growing areas. Thirty four cultivars and nineteen strains of the Chinese cabbage were planted in the contaminated fields in highland and investigated the wilting symptom due to the initial disease occurrence and high temperature. Disease occurrence rate and severity of clubroot and the horticultural characteristics were also examined. According to the results, Bulamplus, Kangryeokyeoleumbaechu among cultivars of Chinese cabbage, SP-05, SP-02, CR DB 50, and CR DB 29 among strains of Chinese cabbage were resistant to hot weather by showing delayed above-ground symptom (wilting) of clubroot in 30 days after transplanting. In case of below-ground clubroot rate, Daetong, super CR Kimi 85 and super CR Hiroki among the cultivars of Chinese cabbage were resistant to clubroot in 60 days after transplanting. When fresh weight of Chinese cabbage among cultivars were compared, super CR Hiroki of over 2,500 g was the highest among other cultivars. Although the resistant and susceptible reactions of Chinese cabbage cultivars and strains against clubroot disease were somewhat different from the above-ground symptoms (wilting) of clubroot in 30 days and below-ground symptoms of clubroot in 60 days, we recommend that super CR Hiroki, CR Kimi 85, CR Janggun among cultivars of Chinese cabbage and CR DB 50 and CR DB 29 among strains of Chinese cabbage will be more suitable in cultivation of Chinese cabbage in highland.

• Korean Journal of Organic Agriculture
• /
• v.23 no.4
• /
• pp.829-846
• /
• 2015

This study was conducted to determine the effects of organic fertilization rates on the nutrient accumulation and recovery in radish (Raphanus sativus L.) as well as growth and yield of radish in Jeju island. An understanding the relationships between organic fertilization rate, crop nutrient recovery and crop yield can assist in making organic fertilizer recommendation which balances crop value and environmental risk in organic cultivation. Nitrogen (T-N), phosphate ($P_2O_5$) and potassium ($K_2O$) were applied at 0, 115-35-40, 230-70-80 (standard application rate), 460-140-160, 230-200-100 (recommended application rate) and 158-53-35 kg/ha (customary application rate), respectively as the broadcast application of mixed organic fertilizer (N 4.5% - $P_2O_5$ 1.5% - $K_2O$ 1%) in combination with langbeinite ($K_2O$ 22%), 100% at sowing period. The organic fertilizer was made of organic materials like oil cakes. Total yield of radish, as fresh weight of roots, increased with increasing organic fertilizer doses to a maximum at rate of standard or soil-testing application. Nitrogen, phosphate and potassium accumulations of radish increased curvilinearly with increasing organic fertilization rate to a maximum at rate of N 460 - $P_2O_5$ 140 - $K_2O$ 160 kg/ha. However, nitrogen, phosphate and potassium use efficiency of applied organic fertilizer decreased curvilinearly or linearly with increasing organic fertilization rate. Application of organic fertilizer in combination with langbeinite (as a potassium source) had significant effect on the yield of radish. Organic fertilization on a basis of standard or soil-testing application rate is recommended for maximun radish yield in organic cultivation.

• Journal of Korean Society of Forest Science
• /
• v.101 no.4
• /
• pp.613-618
• /
• 2012

The 5-year-old seedlings and emblings which regenerated from somatic embryos were compared to the height, DBH, foliar characteristics, content of chlorophyll (chlorophyll a, b and total chlorophyll), carotenoid and leaf microstructure in Liriodendron tulipifera. In comparison of height and DBH (diameter at breast height), no significant differences were found in height (seedling, 3.8 m; embling, 3.87 m) and DBH (seedling, 12.09 cm; embling, 12.53 cm). The emblings and seedlings were similar in values of length (seedling, 108.11 mm, embling, 113.59 mm), width (seedling, 149.1 mm; embling, 167.71 mm), surface area (seedling, $119.92mm^2$; embling, $164.43mm^2$), fresh weight (seedling, 2.1 g; embling, 2.62 g) of leaf, and length (seedling, 81.49 mm; embling, 98.41 mm) and thickness (seedling, 1.66 mm; embling, 1.98 mm) of petiole. In case of chlorophyll content in the leaves, the chlorophyll a (seedlings, $11.2{\mu}g/g$; emblings, $13.2{\mu}g/g$), b (seedlings, $4.8{\mu}g/g$; emblings, $5.4{\mu}g/g$) and total content were higher in emblings ($930.2{\mu}g/g$) than seedlings ($800.1{\mu}g/g$), however, content of carotenoid (seedlings, $260.3{\mu}g/g$; embling, $265.2{\mu}g/g$) showed similar in both plants. Leaves of emblings had a similar pattern of histological structure (palisade or sponge parenchyma) to that of seedlings leaves. Therefore, the results showed that there were no remarkable growth differences when compared with 5-year-emblings and seedlings of yellow poplar.

• Journal of Bio-Environment Control
• /
• v.18 no.1
• /
• pp.15-22
• /
• 2009

Adequate environment conditions with growth stage of potato were decided in a photoautotrophic micropropagation system using models. Total 20 day-period of growth were divided into three growth periods such as 6 (stage 1), 7(stage 2), and 7(stage 3) days. At the 1st stage, no significant differences were observed in the growth of potato plantlets at various photosynthetic photon flux density (PPFD) and $CO_2$ conditions. Considering damaged leaves, $80\;mmol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD and ambient $CO_2$ level were adequate in this stage. At the 2nd stage, significant differences were partly observed in several growth characteristics including dry weight. Based on the dry matter model, over $240\;mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD was too high to cultivate potato plantlets at this stage due to the occurrence of damaged leaves. Considering both plant growth and energy efficiency, $160\;mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD and $700\;mol{\cdot}mol^{-1}\;CO_2$ were selected for the adequate combination. At the 3rd stage, the biomass accumulation was significantly induced in potato plantlets under higher levels of PPFD and $CO_2$ concentration as suggested by increased fresh and dry weights. However, we could not find the saturated point with regard to dry matter due to continuous increase of dry mater even under maximum PPFD ($320\;mmol{\cdot}m^{-2}{\cdot}s^{-1})$. Thus, $320\;mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD and $1800\;mol{\cdot}mol^{-1}\;CO_2$ were considered as the best choice at final stage in this study. In conclusion, even though the growth period of micropropagated potato plantlets was quite a short, favorable environmental conditions required at each growth stage were different. This technique could improve the growth of micropropagated plantlets compared to the conventional micropropagation and apply to other agriculturally important crops as well as potato in the future.

• Journal of Bio-Environment Control
• /
• v.22 no.2
• /
• pp.146-153
• /
• 2013

This study was conducted to enhance graft-take ratio and quality of grafted tomato seedlings by controlling temperature and humidity during the healing and acclimatization processes. Three temperature levels ($20^{\circ}C$, $23^{\circ}C$, and $26^{\circ}C$) were carried out to determine optimum temperature on four rootstocks. In addition, twelve combinations of three relative humidity levels (70%, 80%, and 90%) and four temperature levels ($17^{\circ}C$, $20^{\circ}C$, $23^{\circ}C$, and $26^{\circ}C$) were set up to evaluate the effect of relative humidity and temperature on the graft-take ratio of grafted seedlings. In the other hand, five relative humidity periods (H0, H1, H2, H3, and H4: 90% relative humidity for first 0, 1, 2, 3 and 10 days and afterwards relative humidity was reduced to 70%, respectively) were examined effect of relative humidity periods on the graft-take and quality of grafted seedlings. The higher graft-take ratios (84.0~87.4%) were showed at $23^{\circ}C$ compared to $20^{\circ}C$ and $26^{\circ}C$ in all rootstocks. Graft-take ratios decreased and number of diseased plants increased at high temperature. The graft-take ratios increased with increasing relative humidity in all temperature levels on the $3^{rd}$ and $7^{th}$ day after grafting. However, increasing relative humidity significantly increased percent of diseased plants. The graft-take ratio reduced at ($26^{\circ}C$) and ($17^{\circ}C$) temperature under all relative humidity conditions. The graft-take ratio increased with increasing period of 90% relative humidity. Maximum graft-take ratios were observed in H2 and H3 treatments. Graft-take ratio decreased with increasing 90% relative humidity for 10 days (H4). Diseased plants had not been found in H0, H1, H2, and H3 treatments. Seedling quality was improved through increasing fresh and dry weight of root, compactness, and root morphology of tomato seedlings in H2 and H3 treatments. Therefore, high relative humidity (90%) for first 2 or 3 days and afterwards reduced low relative humidity (70%) at $23^{\circ}C$ condition during healing and acclimatization promoted the graft-take and quality of grafted tomato seedlings.

• Journal of Bio-Environment Control
• /
• v.26 no.3
• /
• pp.194-200
• /
• 2017

This study aimed to determine the suitable of harvest time on the growth and quality of baby leafy vegetables (Agastsche rugosa O. Kuntze and Lepidium sativum L.) grown on rice seedling tray in a six-layered bench system at 30cm intervals in order to exploit the space during rice growing off-season. Seedlings were grown on the rice seedling tray for 10 days after sowing with coir substrate supplied with nutrient solution at EC $1.5dS{\cdot}m^{-1}$ every 2 days prior to placing the tray on the bench, which were at $1^{st}$ (Low) layer above 20cm and $6^{th}$ (High) layer above 170cm apart from the ground. Growth and phytochemical contents were measured at 7-day and 14-day harvest time. During the culture periods, daily average of integrated solar radiation and temperature were $9.3{\sim}9.6MJ{\cdot}m^{-2}$, $27.5^{\circ}C$ in the High layer and $5.1{\sim}6.2MJ{\cdot}m^{-2}$ in average, and $26.5{\sim}26.6^{\circ}C$ in the Low layer, respectively. For A. rugosa, the highest growth was observed in the Low layer bench at a 14-day harvest time, while their plant height in the High layer was shorter and the leaf number was lower. For L. sativum, the plant height, leaf length and width, leaf number and fresh weight were higher in the Low layer. For A. rugosa, a high yield was observed with the increase in integrated temperature and integrated solar radiation, while a higher yield of L. sativum was found with the increase in integrated temperature, but not with integrated solar radiation. For A. rugosa, both polyphenol and anthocyanin contents were higher in the High layer at a 14-day harvest time. For L. sativum, polyphenol contents were higher in the High layer, whereas no significant difference in anthocyanin and flavonoid contents was observed depending on the layer and harvest time. The highest chlorophyll content showed in Low layer at a 7-day harvest time in both A. rugose and L. sativum. All of the results suggest that in terms of growth and quality, it may be better growing in the high layer for 14 days after seedling in A. rugosa, and low layer for 7 days in L. sativum.