Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
권호 표지
DOI QR코드

DOI QR Code

Optimalization of Flurprimidol Concentration for Improvement of Acclimatization Rate and In Vitro Growth Inhibition in Bioreactor Culture of Strawberry Plantlets via Bioreactor Culture

딸기 조직배양묘의 생물반응기 배양 시 Flurprimidol 처리농도에 따른 기내 생장억제 및 순화율 향상

  • Kim, Hye Jin (Highland Agricultural Research Center, National Institute of Crop Science, RDA) ;
  • Lee, Jong Nam (Highland Agricultural Research Center, National Institute of Crop Science, RDA) ;
  • Kim, Ki Deog (Highland Agricultural Research Center, National Institute of Crop Science, RDA) ;
  • Kwon, Gi Bum (Highland Agricultural Research Center, National Institute of Crop Science, RDA) ;
  • Yoo, Dong Lim (Highland Agricultural Research Center, National Institute of Crop Science, RDA) ;
  • Lim, Hak Tae (Department of Bio-Health Technology, Kangwon National University) ;
  • Yeoung, Young Rok (Department of Plant Science, Gangneung-Wonju National University)
  • 김혜진 (국립식량과학원 고령지농업연구센터) ;
  • 이종남 (국립식량과학원 고령지농업연구센터) ;
  • 김기덕 (국립식량과학원 고령지농업연구센터) ;
  • 권기범 (국립식량과학원 고령지농업연구센터) ;
  • 유동림 (국립식량과학원 고령지농업연구센터) ;
  • 임학태 (강원대학교 생명건강공학전공) ;
  • 용영록 (강릉원주대학교 식물생명과학과)
  • Received : 2014.04.02
  • Accepted : 2015.04.01
  • Published : 2015.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to improve the acclimatization rate of in vitro strawberry plantlets through bioreactor culture using the growth retardant flurprimidol. Different concentrations [0 (Control), 0.1, 0.5, 1.0, and $2.0mg{\cdot}L^{-1}$] of flurprimidol were added during bioreactor culture. After six weeks of treatments, various growth characteristics were investigated and in vitro plantlets were acclimated in the greenhouse. The growth rate of treated plantlets was much lower than that of control, and as the treatment concentration increased, the growth rate was much decreased. Shoots of plantlets treated with flurprimidol were shorter (2.2-3.7 cm) than those of control (7.9 cm). The number of roots per treated plant was around 11.6-34.2, compared with 51.8 in the control. Root length was also lower (0.88-3.08 cm) than control (4.36 cm). However, the number of new shoots and leaves increased in all treatments except for $2.0mg{\cdot}L^{-1}$ concentration. The root was partially decayed in $1.0mg{\cdot}L^{-1}$ concentration and was completely decayed in $2.0mg{\cdot}L^{-1}$. The survival rate in $0.1mg{\cdot}L^{-1}$ and $0.5mg{\cdot}L^{-1}$ concentrations was 100% and 23.3% respectively. After four weeks of acclimatization, the plantlets restarted growth, and growth characteristics of shoots and roots recovered to the levels of control, except for fresh weight. Based on our results, a concentration of $0.1mg{\cdot}L^{-1}$ flurprimidol is appropriate for improvement of acclimatization rate of in vitro strawberry plantlets in bioreactor culture.

본 실험은 생물반응기를 이용한 딸기 조직배양묘 대량증식 시 flurprimidol 을 처리하여 순화율을 향상시키고자 실시하였다. Flurprimidol 을 0, 0.1, 0.5, 1.0 및 $2.0mg{\cdot}L^{-1}$의 농도로 6주동안 처리하여 생육조사한 결과, flurprimidol을 첨가한 처리구의 생장량이 대조에 비해 감소하였고, 처리농도가 증가할수록 생장억제효과가 크게 나타났다. 초장은 대조가 7.9cm인 것에 비해 flurprimidol 처리는 2.2-3.7cm 범위로 매우 짧아졌다. 뿌리수는 대조가 51.8개/주에 비해 flurprimidol 처리가 11.6-34.2개/주 범위로 감소하였다. 뿌리길이는 대조가 4.36cm에 비해 flurprimidol 처리는 0.88-3.08cm 범위로 크게 짧아졌다. 그러나 액아수는 flurprimidol $2.0mg{\cdot}L^{-1}$ 처리를 제외하고 모든 처리구에서 대조의 8.6개/주에 비해 많이 발생하였을 뿐만 아니라 액아수가 증가할수록 그에 따라 잎수도 증가하였다. Flurprimidol $1.0mg{\cdot}L^{-1}$ 처리 시 뿌리가 부패하여 지하부 발달이 불량하였다. Flurprimidol $1.0mg{\cdot}L^{-1}$와 $2.0mg{\cdot}L^{-1}$ 처리를 제외한 모든 처리의 순화율은 100%로 대조에 비해 23.3% 향상되었다. 순화 4주 후, flurprimidol $0.1mg{\cdot}L^{-1}$ 처리는 생체중을 제외한 지상부 및 지하부 생육이 대조와 비슷하게 회복되었다. 따라서 생물반응기를 이용한 딸기의 조직배양묘 대량 증식 시 순화율 향상을 위한 적정 flurprimidol 농도는 $0.1mg{\cdot}L^{-1}$였다.

Keywords

References

  1. Berruti, A. and V. Scariot. 2013. Efficacy of flurprimidol and peat alternatives on growth control of potted camellias. N. Z. J. Crop Hortic. Sci. 41:230-239. https://doi.org/10.1080/01140671.2013.811091
  2. Chen, J. and M. Ziv. 2001a. Ancymidol effect on oxidative stress and the regeneration potential of Narcissus leaves in liquid culture. Acta Hortic. 560:299-302.
  3. Chen, J. and M. Ziv. 2001b. The effect of ancymidol on hyperhydricity, regeneration, starch and antioxidant enzymatic activities in liquid-cultured Narcissus. Plant Cell Rep. 20:22-27. https://doi.org/10.1007/s002990000283
  4. Ellington, E., T. Adserias, A. Coma, J. Bastida, F. Viladomat, and C. Codina. 1997. Effect of paclobutrazol on in vitro culture of Colchicum autumnale corms. Acta hortic. 447:131-133.
  5. Graebe, J.E. 1987. Gibberellin biosynthesis and control. Annu. Rev. Plant Physiol. 381:419-465.
  6. Guen-Le Saos, F., A. Hourmant, F. Esnault, and J.E. Chauvin. 2002. In vitro bulb development in schaliot (Allium cepa L. Aggregatum Group): effects of anti-gibberellins, sucrose and light. Ann. Bot. 89:419-425. https://doi.org/10.1093/aob/mcf063
  7. Hazarika, B.N. 2003. Acclimatization of tissue -cultured plants. Current Sci. 85:1704-1712.
  8. Ilczuk, A., T. Winkelmann, S. Richartz, M. Witomska, and A. Serek. 2005. In vitro propagation of $Hippeastrum\;{\times}\;chmielii\;Chm.$ - influence of flurprimidol and the culture in solid or liquid medium and in temporary immersion systems. Plant Cell, Tissue Organ Cult. 83:339-346. https://doi.org/10.1007/s11240-005-8812-5
  9. Kim, E.K., E.J. Hahn, H.N. Murthy, and K.Y. Paek. 2003. High frequency of shoot multiplication and bulblet formation of garlic in liquid cultures. Plant Cell, Tissue Organ Cult. 73:231-236. https://doi.org/10.1023/A:1023029302462
  10. Kim, H.J., J.N. Lee, K.D. Kim, J.S. Im, H.T. Lim, and Y.R. Yeoung. 2011. Suitable hormone-free medium for in vitro mass propagation via bioreactor culture of ever-bearing strawberry. J. Plant Biotechnol. 38:221-227. https://doi.org/10.5010/JPB.2011.38.3.221
  11. Lee, J.N., H.J. Kim, K.D. Kim, Y.S. Kwon, J.S. Im, H.K. Lim, and Y.R. Yeoung. 2010. In vitro mass propagation and economic effects of bioreactor culture in ever-bearing strawberry 'Goha'. Kor. J. Hort. Sci. Technol. 28:845-849.
  12. Lee, J.N., H.J. Kim, K.D. Kim, J.S. Im, H.T. Lim, and Y.R. Yeoung. 2012. Growth characteristics and economic efficiency of nursery plants production according to transplanting container for acclimatization of mass propagated plantlets via bioreactor culture of ever-bearing strawberry 'Goha'. Kor. J. Hort. Sci. Technol. 30:437-441.
  13. McClelland, M.T. and M.A.L. Smith. 1990. Vessel type, closure, and explants orientation influence in vitro performance of five woody species. Hortic. Sci. 25:797-800.
  14. Sansavini, S. P. Rosati, D. Gaggioli, and M.F. Toshi. 1990. Inheritance and stability of somaclonal variation in micropropagated strawberry. Acta Hortic. 280:375-384.
  15. Smith, E.F., A.V. Roberts, J. Mottley, and S. Denness. 1991. The preparation in vitro of chrysanthemum for transplantation to soil. 4. The effect of eleven growth retardants on wilting. Plant Cell, Tissue Organ Cult. 27:309-313. https://doi.org/10.1007/BF00157596
  16. Sutter, E.G. 1988. Stomata and cuticular water loss from apple, cherry, and sweet-gum plants after removal from in vitro culture. J. Am. Soc. Hortic. Sci. 113:234-238.
  17. Sutter, E.G. and R.W. Langhans. 1979. Epicuticular wax formation on Carnation plantlets regenerated from shoot-tip culture. J. Am. Soc. Hortic. Sci. 104:493-496.
  18. Wardle, K., E.B. Dobbs, and K.L. Short. 1983. In vitro acclimatization of aseptically cultured plantlets to humidity. J. Am. Soc. Hortic. Sci. 108:386-389.
  19. Ziv, M. 1989. Enhanced shoot and cormlet proliferation in liquid cultured Gladiolus buds by growth retardants. Plant Cell, Tissue Organ Cult. 17:101-110. https://doi.org/10.1007/BF00046855
  20. Ziv, M. 1990. The effect of growth retardants on shoot proliferation and morphogenesis in liquid cultured Gladiolus plants. Acta Hortic. 280:247-258.