• Journal of Microbiology and Biotechnology
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• v.27 no.2
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• pp.289-296
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• 2017

Lysine decarboxylase (CadA) converts ${\small{L}}-lysine$ into cadaverine (1,5-pentanediamine), which is an important platform chemical with many industrial applications. Although there have been many efforts to produce cadaverine through the soluble CadA enzyme or Escherichia coli whole cells overexpressing the CadA enzyme, there have been few reports concerning the immobilization of the CadA enzyme. Here, we have prepared a cross-linked enzyme aggregate (CLEA) of E. coli CadA and performed bioconversion using $CadA^{CLEA}$. $CadA^{free}$ and $CadA^{CLEA}$ were characterized for their enzymatic properties. The optimum temperatures of $CadA^{free}$ and $CadA^{CLEA}$ were $60^{\circ}C$ and $55^{\circ}C$, respectively. The thermostability of $CadA^{CLEA}$ was significantly higher than that of $CadA^{free}$. The optimum pH of both enzymes was 6.0. $CadA^{free}$ could not be recovered after use, whereas $CadA^{CLEA}$ was rapidly recovered and the residual activity was 53% after the $10^{th}$ recycle. These results demonstrate that $CadA^{CLEA}$ can be used as a potential catalyst for efficient production of cadaverine.

• Proceeding of EDISON Challenge
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• 2015.03a
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• pp.541-542
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• 2015

Recently, a popularity of the smartphone is on the rise. And also related services are being created. The growth of the smart technology makes advanced equipment for smartphone accessories. Selfie-rod is one of them. Selfie-rod satisfies people who want to take their own picture at long distance. However, the weight of recent selfie-rods is too heavy that has nice functions. So, we have re-designed new selfie-rod that has light-weight body. First, we observed a selfie-rod and check the reason of the selfie-rod's high weight. The new selfie-rod has a shell and inside, there is a space that it can be light-weight. The shell and space make the selfie-rod lighter. In FreeCAD, we designed shell with a rotation function. Finally, we designed light-weight body selfie-rod with FreeCAD. FreeCAD can be used easily for beginners of CAD. FreeCAD is suitable 3D-design tool for era of 3D-printing.

• Proceeding of EDISON Challenge
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• 2015.03a
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• pp.538-540
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• 2015

In this paper, We have conducted a Compact SELCA-Rod design by using FreeCAD. FreeCAD is many modern design applications such as Revit or CATIA. It is based on the concept of Workbench. A Workbench can be considered as a set of tools specially grouped for a certain task. Also It has an extensive list of features FreeCAD implements. We use this practical tool and proceed the research on Compact SELCA-Rod which is attracted the most IT-related products in 2014. SELCA-Rod is mostly based on rod-shaped form as in name. However rod-shaped design has the biggest problem for mobile Through this research, We propose a new type of SELCA-Rod. It is easy to carry and take pictures from different angle.

• Proceeding of EDISON Challenge
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• 2015.03a
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• pp.452-459
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• 2015

Thanks to the development of applications, many people are using a smart phone cradle these days. But it has some problems. First of all, it is hard to fix on automobiles. Also, it is vulnerable to external vibration. Recognizing these problems, we decided to improve this device by using FreeCAD and ANSYS. Because FreeCAD is newly invented software, we used another 3D modeling software to cover shortages of tools in FreeCAD. Also, we used famous analysis software 'ANSYS' to analyze bending deformation, modal analysis, and harmonic analysis.

• Proceeding of EDISON Challenge
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• 2015.03a
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• pp.512-513
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• 2015

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1993.10a
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• pp.417-422
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• 1993

In order to minimize turnaround of machining in FMS lines, CAD/CAM/CAT integrated system called MascCAM was developed. Developing enhanced CAM and inspection modules in the MascCAM environment, 2D came, 2 $^{1}$2/ D prismatic parts and 3D free-formed surfaces were able to be automatically designed, manufactured and inspected on the machine tools by using AutoCAM and Z-map. Introducing Z-map technique, the MascCAM was able to be interfaceed with and CAD system. Developed QPPGT module generates a quick and fool-proof inspection work to users. A vertical and a horizontal machining center equipped with FANUC OMC were used for experiments. Performance of the system was confirmed by a large amount of experiments.

• Proceeding of EDISON Challenge
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• 2015.03a
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• pp.428-441
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• 2015

Corresponding to the high consumption rate of the selfie stick for cell phone camera, the design of the product is becoming diverse. However, relatively low cost selfie stick models are not strong enough to stand the weight of the cell phone. This results into a bending of the stick or the accidental fall of the cell phone by the failure of the holding unit. As a solution to the problem, design of selfie stick that is structurally strong enough to stand the weight of the cell phone with the minimum weight for the portability is proposed in this paper.

• Journal of the Korean Society of Costume
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• v.41
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• pp.23-47
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• 1998

Ornaments is a province of the fashion. It usually express noble metals and jewel's adorn-ment as the symbol of status and riches for a long time. The ornament design of Jewels drawing project and a product by computer are increased development and as exertion as a realization of automation. Through the use of CAD software(Auto CAD R 13 & Jewel CAD) \circled1 A design development of the jewels in industrial society \circled2 A metals art & design on the dress and it's ornaments - Study about application of principle(liberal curve, arrangement of repeated form, gradual unity, rhythmical harmony) Although Auto CAD don't various expression of Jewels than a Jewel CAD, formative.scientific.funtional development of geometrical form is free. That is (to say), geometrical form is given much weigh in the general CAD, but Jewel CAD made concentrate software on the jewels design for the expression of liberal form. The CAD/CAM software for jewellery program is composed of main menu, icons, hotkeys. Changing form is derived from a definite point, curve elements of a drawing. \circled1 3-Dimensional \circled2 Easy and flexible \circled3 Bulit-in and self created library \circled4 From simple wire frame to full color images. As a CAD can practice all the creation activity effectively, from Design & Drafting Software to Rendering generally can present precise results. A point of view of the connection the scientist and art, this practicableness of CAD have a lot of possiblity of development. That will do much for the related fields of industry. Consequently, subjective intension of a creator & humanity with value plays role in practical application of the design.

• CDE review
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• v.21 no.2
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• pp.50-60
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• 2015

Corresponding to the high consumption rate of the selfie stick for cell phone camera, the design of the product is becoming diverse. However, relatively low cost selfie stick models are not strong enough to stand the weight of the cell phone. This results into a bending of the stick or the accidental fall of the cell phone by the failure of the holding unit. As a solution to the problem, design of selfie stick that is structurally strong enough to stand the weight of the cell phone with the minimum weight for the portability is proposed in this paper.

• Journal of the Korean Society for Precision Engineering
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• v.17 no.9
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• pp.53-61
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• 2000

The manufacture of a marine propeller typically requires long lead time to generate 5-axis tool path. Hence it may take several weeks to manufacture a satisfactory propeller with a general purpose CAD/CAM system. In this research a dedicated 5-axis CAD/CAM system for machining marine propellers has been developed, The system employs various methods to enhance the productivity : interference-free tool path generation employing check vectors and optimum cutter size determinants. In addition an iterative NURBS modeling technique is used to improve the accuracy of the modeled surface and effective cutting conditions are determined and recommended empirically to increase the productivity. The proposed CAD/CAM system has been implemented with C++ and OpenGL graphic library on the Windows system. The system validation and sample results are also given and discussed.