• Journal of Microbiology and Biotechnology
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• v.25 no.10
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• pp.1621-1628
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• 2015

Chlamydia possesses a conserved 7.5 kb plasmid that is known to play an important role in chlamydial pathogenesis, since some chlamydial organisms lacking the plasmid are attenuated. The chlamydial transformation system developed recently required the use of plasmid-free organisms. Thus, the generation and identification of plasmid-free organisms represent a key step in understanding chlamydial pathogenic mechanisms. A tricolor immunofluorescence assay for simultaneously detecting the plasmid-encoded Pgp3 and whole organisms plus DNA staining was used to screen C. muridarum organisms selected with novobiocin. PCR was used to detect the plasmid genes. Next-generation sequencing was then used to sequence the genomes of plasmid-free C. muridarum candidates and the parental C. muridarum Nigg strain. We generated five independent clones of plasmid-free C. muridarum organisms by using a combination of novobiocin treatment and screening plaque-purified clones with anti-Pgp3 antibody. The clones were confirmed to lack plasmid genes by PCR analysis. No GlgA protein or glycogen accumulation was detected in cells infected with the plasmid-free clones. More importantly, whole-genome sequencing characterization of the plasmid-free C. muridarum organism and the parental C. muridarum Nigg strain revealed no additional mutations other than loss of the plasmid in the plasmid-free C. muridarum organism. Thus, the Pgp3-based immunofluorescence assay has allowed us to identify authentic plasmid-free organisms that are useful for further investigating chlamydial pathogenic mechanisms.

• Journal of the Korean Institute of Landscape Architecture
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• v.43 no.5
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• pp.100-110
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• 2015

With the trend for the construction of barrier-free living environments in accordance with the ever-increasing external activity of the aging population, multi-culture families and socially disadvantaged, there is also a demand for the facilities improvement at parks for their convenience of use. This study analyzed the possibility of Barrier-Free Certification of an existing open space and defined its prototype renovation plan. This study is intended to help develop Borame Park into a barrier-free park by securing a barrier-free line of movement linking the major facilities sites within the park, improving park facilities lest there should be inconvenience in the use of the major facilities, and a guidance system convenient for use by physically and mentally impaired people. The significance of this study lies in the fact that it diagnosed the possibility of the BF Certification of existing urban parks and established a barrier-free park, which is easy for anyone to use. In the course of doing research on this, this study was able to discover the limitations of the BF Certification system, and its related tasks to be solved in the future as follows: The first thing this study shows is that there is a lack of understanding and awareness of the BF Certification system. Basically, there is a need for giving consideration to the development of a barrier-free living environment from the planning and design phase of a park, but in actuality, an examination of the certification is undertaken at the time of the completion of design and construction, which causes a large loss both time-wise and economically. Second, as evaluative items for the BF Certification of a park, applied are detailed evaluation items that fail to reflect the characteristics of an outdoor space whose natural constraints are large, such as geographical highs and lows and existing trees, plants, etc. Third, guidelines for establishment of a barrier-free park must be created in the light of the locational conditions of the park, followed by improvements of its BF Certification evaluation items.

• Korean Journal of Head & Neck Oncology
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• v.23 no.1
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• pp.26-31
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• 2007

Background and Objectives：Microvascular free flap reconstruction has been revolutionized in last two decades, and became a standard option in the reconstruction of head and neck defects. We intended to review our experiences of 51 microvascular free flap for head and neck defects during 5-year period and to analyze the types of flaps according to primary sites, success and complication rates. Subjects and Methods：From Oct. 2001 through Dec. 2005, fifty one free flap reconstructions were performed in forty nine patients at ENT department of Soonchunhyang university bucheon hospital. Primary sites, pathology, T-stage, operative time, time interval of oral feeding, and various reconstructive factors such as recipient and donor vessels, free flap related complications, failure rates and salvage rates were retrospectively analyzed. The relation between complication rates and preoperative risk factors were statistically analyzed. Results：Methods of reconstruction were radial forearm free flap(RFFF)(n=28, 54.9%), anterolateral thigh free flaps(n=9, ALTFF)(17.6%), rectus abdominis free flap(n=7, RAFF)(13.7%), jejunal free flap(n=5, JFF)(9.8%), and miscellanous(n=2, 4.0%) in order. In free flap related complications, failure of free flap occurred in seven cases(13.7%) and pharyngocutaneous fistula occurred in five cases(9.8%) among fifty one free flaps. The overall success rate of free flaps was 86.3%. Salvage of free flaps was possible only one among eight cases(12.5%). In positive preoperative risk factor groups, failure of free flap was higher than in negative risk factor group. However, it was not statistically significant. Conclusion：We confirmed that free flap reconstructions are highly versatile and reliable options for use in the reconstruction of various soft tissue defects of the head and neck. Free flaps have gained great popularity given its versatility, ability for a two-team approach, and minimal donor site morbidity. However, complications related to microvascular surgery may be overcome by increased surgical experience and by intensive flap monitoring in early postoperative period.

• 대한미세수술학회:학술대회논문집
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• 1999.11a
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• pp.217-218
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• 1999

• Journal of computational fluids engineering
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• v.13 no.4
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• pp.13-23
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• 2008

This paper is an extension of previous study[1] on a development of a divergence-free element method using a hermite interpolated stream function. Divergence-free velocity bases defined on rectangles derived herein produce pointwise divergence-free flow fields. Hence the explicit imposition of continuity constraint is not necessary and the Galerkin finite element formulation for velocities does not involve the pressure. The divergence-free element of the previous study employed hermite (serendipity) cubic for interpolation of stream function, and it has been noted a possible discontinuity in variables along element interfaces. This deficiency can be removed by use of a hermite bicubic interpolated stream function, which requires four degrees-of-freedom at each element corners. Those degrees-of-freedom are the unknown variable, its x- and y-derivatives and its cross derivative. Detailed derivations are presented for both solenoidal and irrotational basis functions from the hermite bicubic interpolated stream function. Numerical tests are performed on the lid-driven cavity flow, and results are compared with those from hermite serendipity cubics and a stabilized finite element method by Illinca et al[2].

• The Korean Journal of Applied Statistics
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• v.10 no.1
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• pp.163-175
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• 1997

Randomization of the run order within a block is a technique commonly employed by the experimenters of block designs to avoid biases in the estimates of the effects of interest. In practice, however the experimental responses are sometimes affected by the spatial or temporal position of the experimental units within a block. In such cases, it is preferable to use a systematic ordering of the treatments. It is often possible to find an ordering which will allow the estimation of treatment effects independently of any trend is known as a trend-free block designs. In many idustrial and agricultural experiments, treatments are applied to experimental units sequentially in time or space. This paper begins with a review of concepts and properties of trend-free designs. We, then devise algorithms to generate linear trend-free designs. We extend and modify the existing algorithm which is given by Bradley and Odeh(1988). Also, the algorithm which generate all possible linear trend-free designs in provided.

• Nuclear Engineering and Technology
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• v.41 no.8
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• pp.1045-1052
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• 2009

A calculation model is developed to predict the transient free surface flow on the containment floor following a loss-of-coolant accident (LOCA) of pressurized water reactors (PWR) for the use of debris transport evaluation. The model solves the two-dimensional Shallow Water Equation (SWE) using a finite volume method (FVM) with unstructured triangular meshes. The numerical scheme is based on a fully explicit predictor-corrector method to achieve a fast-running capability and numerical accuracy. The Harten-Lax-van Leer (HLL) scheme is used to reserve a shock-capturing capability in determining the convective flux term at the cell interface where the dry-to-wet changing proceeds. An experiment simulating a sudden break of a water reservoir with L-shape open channel is calculated for validation of the present model. It is shown that the present model agrees well with the experiment data, thus it can be justified for the free surface flow with accuracy. From the calculation of flow field over the simplified containment floor of APR1400, the important phenomena of free surface flow including propagations and interactions of waves generated by local water level distribution and reflection with a solid wall are found and the transient flow rates entering the Holdup Volume Tank (HVT) are obtained within a practical computational resource.

• Environmental Mutagens and Carcinogens
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• v.19 no.2
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• pp.116-121
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• 1999

Quercetin and its glycosides showed a strong free radical scavenging effect to DPPH radical generation. However, there were not big differences between quercetin aglycone and glycosides under experimental condition of this study. On the other hand, quercetin had pro-oxidant effect in bleomycin-dependent DNA assay. Quercetin aglycone and its glycosides, quercitrin inhibited $H_2$$O_2$- induced DNA damage in CHL cells. They also have an anticlastogenicity toward DNA breakage agent by radical generation like bleomycin. These results indicate that quercetin aglycone and its glycosides are capable of protecting the free radical generation induced by reactive oxygen species like $H_2$$O_2$. The mechanism of inhibition in hydrogen peroxide-induced genotoxicity may be due to their free radical scavenging properties. Therefore, quercetin aglycone and its glycosides may be useful chemopreventive agents by protecting of free radical generation which are involved in carcinogenesis and aging. However, quercetin and its glycosides must also carefully examined for pro-oxidant properties before being proposed for use in vivo.

• 한국전산유체공학회:학술대회논문집
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• 2010.05a
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• pp.326-334
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• 2010

In this study, we provide a comprehensive review of the CIP(Constrained Interpolation Pro file/Cubic Interpolated Propagation) method with a pressure-based algorithm that is known as a general numerical solver for soled liquid, gas and plasmas. And also we introduce a body-fitted grid system(Soroban grid) for computation of strongly nonlinear marine hydrodynamic problems such as slamming water on deck, wave impact by green water. This grid system can keep the third-order accuracy in time and space with the help of the CIP method. The grid system consists of the straight lines and grid points. In the 2-dimensional grid case, each grid points moving in these lines like abacus - Soroban in Japanese. The length of each line can be different and the number of grid points in each line can be different. Mesh generation and searching of upstream departure point are very simple and possible to mesh-free treatment. To optimize computation of free-surface and multi-fluid flows, We adopt the C-CUP method. In most of the earlier computations, the C-CUP method was used with a staggered-grid approach. Here, because of the mesh free nature of the Soroban grid, we use the C-CUP method with a collocated-grid approach.

• 한국전산유체공학회:학술대회논문집
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• 2008.03a
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• pp.33-41
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• 2008

This paper is an extension of previous study[9] on a development of a divergence-free element method using a hermite interpolated stream function. Divergence-free velocity bases defined on rectangles derived herein produce pointwise divergence-free flow fields. Hence the explicit imposition of continuity constraint is not necessary and the Galerkin finite element formulation for velocities does not involve the pressure. The divergence-free element of the previous study employed hermite serendipity cubic for interpolation of stream function, and it has been noted a possible discontinuity in variables along element interfaces. This deficiency can be removed by use of a hermite bicubic interpolated stream function, which requires at each element corners four degrees-of-freedom such as the unknown variable, its x- and y-derivatives and its cross derivative. Detailed derivations are presented for both solenoidal and irrotational bases from the hermite bicubic interpolated stream function. Numerical tests are performed on the lid-driven cavity flow, and results are compared with those from hermite serendipity cubics and a stabilized finite element method by Illinca et al[7].