• Title/Summary/Keyword: Free Amino Acid

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Feeding Introlerance Due to Allergic Enterocolitis in Very Low Birth Weight Infants (극소 저체중 출생아에서 식이 불내성을 임상적 특징으로 하는 알레르기성 장염)

  • Ko, Yu-Mi;Kim, Jin-Kyu;Ahn, So-Yoon;Yoo, Hye-Soo;Jeon, Ga-Won;Kim, Eun-Sun;Chang, Yun-Sil;Park, Won-Soon
    • Neonatal Medicine
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    • v.18 no.2
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    • pp.204-210
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    • 2011
  • Purpose: Feeding intolerance is common in very low birth weight infants(VLBWI); however, research on the etiology is limited. We investigated the incidence of allergic enterocolitis (AEC) as a cause of feeding intolerance and present the clinical characteristics of VLBWIs. Methods: The medical records of VLBWIs admitted to the neonatal intensive care unit of Samsung Medical Center between January 2009 and July 2010 were retrospectively analyzed. AEC was defined as patients who had feeding intolerance with eosinophila and who responded to hypoallergenic feeding intervention. Feeding intolerance symptoms included blood tinged stools, abdominal distension, residual feeding and regurgitation. Eosinophilia was defined as an eosinophil count ${\geq}$700 cells/$mm^3$. Patients with feeding intolerance were divided into the AEC or non-AEC group. Results: Of the 181 patients, 161 (88.9%) had a feeding intolerance, and 119 (65.7%) had eosinophilia. Seventeen infants were diagnosed with AEC. No difference in mean gestational age, birth weight, antibiotics duration, TPN duration, hospitalization, or symptom onset day was observed between patients with AEC and non-AEC patients. The percentage of eosinophilia was significantly higher in patients with AEC than in non-AEC patients. Two patients (12%) improved with restricted breast milk, 10 patients (59%) with extensively hydrolyzed formula and five patients (29%) with free amino acid-based formula. Conclusion: Our results suggest that AEC should be considered in VLBWIs who have clinical features of feeding intolerance and eosinophilia. An aggressive increase in feeding would be possible through feeding intervention in VLBWIs with feeding intolerance.

Effect of Inoculation with Bradyrhizobium sp. and Lime Types on Peanut Plant at Newly Reclaimed Area -1. Content of Nutrients in Plant and Yield (야산개간지(野山開墾地)에서 근류균(根瘤菌) 접종(接種) 및 석회(石灰) 비종별(肥種別) 시용(施用)이 땅콩 생육(生育)에 미치는 영향 -1. 식물체중(植物體中) 양분함량(養分含量) 및 수량(收量))

  • Lee, Deog-Bae;Im, Geon-Jae;Kwon, Tae-Oh;Lee, Sang-Bok;So, Jae-Don
    • Korean Journal of Soil Science and Fertilizer
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    • v.24 no.3
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    • pp.210-218
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    • 1991
  • This study was carried out to investigate the influence of inoculation with Bradyrhizobia and lime types on the nutrient content and yield of peanut. Inoculation size of Bradyrhizobium sp. HCR-46 was $8.2{\times}10^7$ cells per seed. Slaked lime, magnesium lime, and calcium carbonate were applied in an amount required for neutralization of soil. 150kg of calcium sulfate was fertilized to 10a of the experimental field. Sowing space was $40{\times}25cm$ under vinyl mulching. Leaves and stems for assay were sampled at 100 day after sowing. The obrained results are as follows. 1. Number and dry weight of nodule as well as dry weight of aerial part of peanut plant increased by inoculation with B. sp. and were the highest in calcium carbonate application. 2. Inoculated with B. sp., the contents of T-N, $K_2O$, MgO, allantoin, ammonia, free amino acid and chlorophyll increased, but that of nitrate decreased. 3. The contents of T-N, free amino acid, and chlorophyll were higher in the treatment of calcium carbonate, those of $K_2O$, MgO, allantoin, ammonia were higher in magnesium lime application, and those of CaO and nitrate were higher in slaked lime fertilization than any other lime types. 4. Contents of total sugar and starch in stem at 100 days were higher in the treatment of uninoculation than inoculation with B. sp., and those were highest in the calcium sulfate application than the other lime types. 5. Inoculated with B. sp. length of main stem and number of pods increased significantiy. Yield of seed was higher in inoculation with B. sp. than in uninoculation by 64%, and in the order of carbonate, magnesium lime, slaked lime, in calcium sulfate and non-application was the contribution of soil treatments to yield increases.

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Quality Characteristics of the Salt-Fermented Oysters in Olive Oil (기름담금 염장발효 굴의 품질특성)

  • Kim, Seok-Moo;Kong, Chung-Sik;Kim, Jong-Tae;Kang, Jeong-Koo;Kim, Nam-Woo;Kim, Jeong-Bae;Oh, Kwang-Soo
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.8
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    • pp.1398-1406
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    • 2004
  • To develop the new type of salt-fermented seafoods, the salt-fermented oysters in olive oil (product SO) were manufactured, and food components and quality characteristics of product SO were examined. The optimum processing condition for product SO is as follows. The raw oyster with no shell was washed off with 3% saline solution. Then dewatered, and dipped in the brine-salting solution made up with saturated saline solution and oyster sauce (2 : 1 v/v) mixture added 1% sodium erythorbic acid and 0.2% polyphosphate. After salt-fermentation it ripened by brine salting at 5$\pm$1$^{\circ}C$ for 15 days. Then dried at 15$^{\circ}C$ for 4 hours with cool-air, and packed in No. 3B hexahedron type can. Finally, poured with olive oil and seamed it by double-seamer. The moisture, crude protein, crude ash and volatile basic nitrogen contents of the product SO were 61.6%, 12.0%, 16.3% and 34.3 mg/100 g, respectively. In taste-active components of the product SO, total amount of free amino acids is 2,335.4 mg/100 g and it has increased by 50% overall during salt-fermentation 15 day. Taurine, glutamic acid, proline, glycine, alanine, $\beta$-alanine and lysine were detected as principal free amino acids. The contents of inorganic ions were rich in Na and K ion, while the amounts of nucleotide and its related compounds and other bases except betaine were small. From the results of this research, the product SO had a superior organoleptic qualities compared with conventional oyster product, and could be reserved in good conditions for storage 90 days at room temperature.

Studies on Processing and Keeping Quality of Retort Pouched Foods (5) Preparation and Keeping Quality of Retort Pouched Seasoned Ark Shell (레토르트파우치 식품의 가공 및 품질안정성에 관한 연구 (5) 레토르트파우치 조미피조개제품의 제조 및 품질안정성)

  • LEE Eung-Ho;OH Kwang-Soo;AHN Chang-Bum;LEE Tae-Hun;CHUNG Young-Hoon;SHIN Keun-Jin;KIM Woo-Jun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.19 no.2
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    • pp.109-117
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    • 1986
  • For the purpose of obtaining basic data which can be applied to processing of retort pouched shellfishes, retort pouched seasoned ark shell, Anadara broughtonii, was prepared. The frozen ark shell was thawed and seasoned with a mixed seasoning powder prepared with $10.0\%$ of sorbitol, $2.0\%$ of table salt and $0.5\%$ of monosodium glutamate at $5^{\circ}C$ for 10 hours, and then dried at $45^{\circ}C$ for 4 hours. The dried seasoned ark shell was coated with $1.0\%$ sodium alginate solution, dried with cola air blast for 2 hours and then vacuum-packed in the laminated plastic film bag (polyester/casted polypropylene= $12{\mu}m/70{\mu}m,\;15{\times}16cm$), and finally sterilized up to Fo=6.0 in hot water circulating retort at $121^{\circ}C$ for 10 minutes. The major fatty acids of raw ark shell and retort pouched seasoned ark shell products were 16:0, 20:5, 22:6, 18:0 and 18:3, and predominant free amino acids of those were lysine, arginine, glycine, alanine, glutamic acid and leucine. In nucleotides and its related compounds of raw ark shell and retort pouched seasoned ark shell products, the most abundant one was AMP, and total extract-N of those was chiefly consisted of free amino acids, betaine and nucleotide and its related compounds. During the processing procedure such as drying and sterilization, unsaturated fatty acids slightly decreased while saturated fatty acids increased, and total extract-N content decreased about a half. From the results of chemical and microbial experiments during storage, it was concluded that the products could be preserved in a good condition for 100 days at room temperature, and their duality could be improved by the coating treatment of sodium alginate solution.

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Changes in Food Components of Dark, White-Fleshed Fishes by Retort Sterilization Processing -1. Changes in Nitrogenous Extractives and Textures- (레토르트 살균처리가 적색육 및 백색육 어류의 성분변화에 미치는 영향 -1. 함질소엑스분 및 텍스튜어의 변화-)

  • OH Kwang-Soo;SUNG Dae-Whan;CHOI Jong-Duck;LEE Eung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.24 no.2
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    • pp.123-129
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    • 1991
  • The skipjack and Alaska pollack meat were packed in square No. 3B can, and thermally processed at $121.1^{\cir}C$ so far as to reach Fo values of 5, 10 and 20, respectively. Also the another samples which were heated at $98^{\cir}C$ for 30 minutes were prepared. We have examined the changes of nitrogenous extractives and texture by thermal processing at high temperature in both fish meats. Moisture contents of both fish meats decreased slightly with the increasing of Fo values. When the samples were heated at $98^{\cir}C$ and $121.1^{\cir}C$, pH and VBN contents increased markedly in both fish meats; increasing ratio of pH and VBN contents in skipjack meat was larger than those of Alaska pollack meat. Histamine were detected in concentration of 6.1mg/100g, 0.1mg/100g in raw skipjack and Alaska pollack meat, 8.2mg/100g, 0.1mg/100g in Fo 20 samples of these fish meats, respectively. The contents of total free amino acids in skipjack and Alaska polack meat were 984.8mg/100g, 282.3mg/100g, and these total contents decreased slightly with increasing of Fo values. Little changes were observed in free amino acid composition, but only His, Tau, Ala in skipjack meat and Tau in Alaska pollack meat decreased significantly by heat treatment. Also the degredation of IMP were Proceeded during Preparation and heat treatment of samples, and TMAO changed into TMA almost equivalently. In changes of textures, as compared with heating of 98t for 30 minutes; Fo 5 heat treatment became more softened these fish meats.

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Functional Properties of Soybean Curd Whey Concentrate by Nanofiltration and Effects on Rheological Properties of Wheat Flour Dough (나노여과에 의한 순물 농축액의 기능적 특성 및 밀가루 반죽의 리올로지 성질에 미치는 영향)

  • Eom, Sang-Mi;Kim, You-Pung;Chang, Eun-Jung;Kim, Woo-Jung;Oh, Hoon-Il
    • The Korean Journal of Food And Nutrition
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    • v.19 no.3
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    • pp.243-253
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    • 2006
  • This study was designed to investigate the feasibility of utilizing concentrates of sunmul(soybean curd whey), the waste by-product of soybean curd processing, as functional food ingredients. Sunmul was concentrated by nanofiltration fo11owing ultrafiltration and then freeze-dried. The oil adsorption capacity of the nanofiltraion(NF) powder(97.33g/100g) was similar to that of sunmul powder(94.17g/100g), but was lower than that of ISP(isolated soy protein). However, the water holding capacity of NF powder could not be determined because the NF powder completely dissolved in water. The protein solubilities of sunmul powder and ISP in distilled H$_{2}$O, 0.1M and 0.5M NaCl were lowest at pH 4.0 and increased at more acidic or alkaline conditions. However, the protein solubility of NF powder was at its minimum at pH 6.0 and increased at more acidic or alkaline conditions. Emulsifying activity indexes of NF powder in 4% and 6% solution were minimal at pH 4.0 and 6.0, respectively, which were 3 to 8 times lower than that of sunmul powder. The emulsion stability of 4% sunmul solution was lowest at pH 4.0, but that of NF powder was highest at pH 5.0 and decreased at more acidic or alkaline conditions at all concentrations of solution. The total free amino acid contents of protein in sunmul, and NF power were 99.07 and 2,110.10mg%, respectively, and NF powder exhibited especially high threonine content. Rapid viscosity analysis of dough with 1 to 5% added NF powder demonstrated that all of the peak and final viscosities decreased with increasing NF powder concentration compared to the control.

Effects of Drainage Depths on Agronomic Characteristics, Yield and Feed Value of Sorghum × Sudangrass Hybrid for Silage in the Paddy Field of Lowland (저지대 논 토양에서 배수로 깊이가 사일리지용 수수×수단그라스 교잡종의 생육특성, 수량 및 사료가치에 미치는 영향)

  • Lee, Sang Moo
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.39 no.2
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    • pp.81-88
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    • 2019
  • This study was carried out to investigate the growth characteristics, yield and feed value of Sorghum${\times}$Sudangrass hybrid for silage according to the drainage depths in the paddy field of lowland. The experimental design was arranged in a randomized block design with four treatments and three replication. The drainage depths of four treatments were 0cm, 20cm, 40cm and 60cm, respectively. Plant length, leaf length and leaf number were not significantly different, but leaf width increased as the higher the drainage depth(p<0.05). The number of dead leaf was higher in the order of $60cm>0cm{\geq}40cm{\geq}20cm$ treatment(p<0.05). Green degree was higher in the order of 20cm > 40cm > 0cm > 60cm treatment(p<0.05). Stem diameter and stem hardness increased significantly as drainage depth increased from 0cm to 60cm(p<0.05). Also, fresh yield, dry matter yield and TDN yield increased as the higher the drainage depth(p<0.05). Crude protein and TDN content were the highest in 40cm treatment(p<0.05). Crude ash was higher in the order of 20cm > 40cm > 0cm > 60cm treatment(p<0.05). ADF and NDF content were the highest in 0cm treatment(p<0.05). Total mineral content was higher in the order of 20cm > 0cm > 40cm > 60cm(p<0.05). Free sugar content(fructose, glucose and sucrose) was the highest in 0cm treatment(p<0.05). Total amino acid(EAA+NEAA) was higher in 40cm than the other treatments(p<0.05). There is a difference in the content of ingredients(crude protein, TDN, mineral, free sugar and amino acid) according to the treatments. But considering dry matter yield and TDN yield, Sorghum${\times}$Sudangrass hybrid cultivation is advantageous to set the drainage depth of about 60cm in the paddy field of lowland.

Effects of the Storage Condition on the Chemical Components of the Fruit Bodies in Oyster Mushroom(Pleurotus ostreatus) (느타리버섯의 저장조건(貯藏條件)이 자실체(子實體)의 화학성분(化學成分)에 미치는 영향(影響))

  • Park, Jeong Sik;Kim, Seong Yeol
    • Korean Journal of Agricultural Science
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    • v.13 no.1
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    • pp.123-129
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    • 1986
  • These studies were conducted to elucidate the effect of storage conditions on the changes of chemical components in fruit bodies of oyster mushroom(Pleurotus ostreatus), and the results obtained were as follows. 1. The fruit bodies of oyster mushroom sealed with polyethlene film 0.03 mm thickness maintained their freshness for 15 days at $1^{\circ}C$, 10 days at $5^{\circ}C$ and 3 days at $20^{\circ}C$. 2. The respiration rates of the fruit bodies grown in the rice-straw substrate was 29.7mg $CO_2/kg$ F.W/hr. at $1^{\circ}C$, 32.7mg at $5^{\circ}C$, and 46.3mg at $20^{\circ}C$ during the storage, respectively. The respiration rate showed the highest level at the second day during the storage. 3. The contents of total and reducing sugar during the storage of oyster mushroom rapidly increased at $5^{\circ}C$ until the fifth day following slowly decreased. 4. The content of protein in the oyster mushroom was reduced during the storage, while the free amino acid slightly increased. 5. The change of RNA and DNA contents during the storage of oyster mushroom showed inconsistency on the temperature and the storage period.

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Effects of Shading and Organic Matter Applications on Growth and Aromatic Constituents of Codonopsis lanceolata (차광과 유기물 시용이 더덕의 생육 및 향기성분에 미치는 영향)

  • 이승필;김상국;남명숙;최부술;이상철
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.4
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    • pp.496-504
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    • 1996
  • The study was conducted to obtain the basic information on increasing aromatic degrees by shading and organic matter application on Condonopsis lanceolata. The result were as followings : Fresh root wt. in shading 55 % treatment was increased about twices, whereas fresh root weight in non-shading was decreased. But growth of ground parts such as vine length, leaf width, and leaf number was promoted under non-shading treatment. Macroelements such as K, Ca, and Mg, were increased in non-shading and higher organic matter application. The components such as crude protein, fiber, and ash were increased in the shading 55% treatment compared with non-shading. In the sixteen amino acids, arginine contents were the highest in non-shading and organic matter 30M/T. Recovery yield of essential oil was 0.005% in non-shading. The highest was organic matter 30M/T in the non-shading as 0.007%. Major aromatic constituents were 11 kinds of aliphatic alcohols such as I-hexanol, cis-3-hexenol, and trans-2-hexanal. As a result, major aromatic degree was higher in shading 55%, also increased in the much organic matter application. It was considered to be applied dense shading 55% and much organic matters to produce Condonopsis lanceolata plants which have high aroma and good quality.

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Apparent and standardized ileal nutrient digestibility of broiler diets containing varying levels of raw full-fat soybean and microbial protease

  • Erdaw, Mammo M.;Perez-Maldonado, Rider A.;Iji, Paul A.
    • Journal of Animal Science and Technology
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    • v.59 no.10
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    • pp.23.1-23.11
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    • 2017
  • Background: Although soybean meal (SBM) is excellent source of protein in diets for poultry, it is sometimes inaccessible, costly and fluctuates in supply. The SBM can partially be replaced by full-fat SBM, but the meals prepared from raw full-fat soybean contain antinutritional factors. To avoid the risk of antinutritional factors, heat treatment is always advisable, but either excessive or under heating the soybean could negatively affect the quality. However, the potential for further improvement of SBM by supplementing with microbial enzymes has been suggested by many researchers. The objective of this study was to evaluate the performance and ileal nutrient digestibility of birds fed on diets containing raw soybeans and supplemented with microbial protease. Methods: A $3{\times}2$ factorial, involving 3 levels of raw full-fat soybean (RFFS; 0, 45 or 75 g/kg of diet) and 2 levels of protease (0 or 15,000 PROT/kg) was used. The birds were raised in a climate-controlled room. A nitrogen-free diet was also offered to a reference group from day 19 to 24 to determine protein and amino acid flow at the terminal ileum and calculate the standardized ileal digestibility of nutrients. On days 10, 24 and 35, body weight and feed leftover were recorded to calculate the body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR). On day 24, samples of ileal digesta were collected at least from two birds per replicate. Results: When RFFS was increased from 0 to 75 g/kg of diet, the content of trypsin inhibitors was increased from 1747 to 10,193 trypsin inhibitors unit (TIU)/g of diets, and feed consumption of birds was also reduced (P < 0.05). Increasing RFFS level reduced the BWG from hatch 0 to 10 d (P < 0.01) and hatch to 24 d (P < 0.05). The BWG of birds from hatch to 35 was not significantly (P = 0.07) affected. Feed intake was also reduced (P < 0.05) during 0 to 35 d. However, protease supplementation improved (P < 0.05) the BWG and FCR during 0 to 24 d. Rising levels of RFFS increased the weight of pancreas (P < 0.001) and small intestine (P < 0.001) at day 24. Except for methionine, apparent and the corresponding standardized ileal digestibility of CP and AA were reduced (P < 0.01) by increasing levels of RFFS in diets. Conclusion: This study showed that some commercial SBM could be replaced by RFFS in broiler diets, without markedly compromising productivity. The AID and SID of CP and lysine were slightly improved by dietary supplementation of microbial protease.