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Development of a Rapid and Productive Cell-free Protein Synthesis System

  • Kim, Dong-Myung;Choi, Cha-Yong;Ahn, Jin-Ho;Kim, Tae-Wan;Kim, Nam-Young;Oh, In-Suk;Park, Chang-Gil
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.3
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    • pp.235-239
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    • 2006
  • Due to recent advances in genome sequencing, there has been a dramatic increase in the quantity of genetic information, which has lead to an even greater demand for a faster, more parallel expression system. Therefore, interest in cell-free protein synthesis, as an alternative method for high-throughput gene expression, has been revived. In contrast to in vivo gene expression methods, cell-free protein synthesis provides a completely open system for direct access to the reaction conditions. We have developed an efficient cell-free protein synthesis system by optimizing the energy source and S30 extract. Under the optimized conditions, approximately $650{\mu}g/mL$ of protein was produced after 2h of incubation, with the developed system further modified for the efficient expression of PCR-amplified DNA. When the concentrations of DNA, magnesium, and amino acids were optimized for the production of PCR-based cell-free protein synthesis, the protein yield was comparable to that from the plasmid template.

Configuration of an IPSec VPN Testbed and Development of an Encryption Verification Tool (IOSec VPN 테스트 베드의 구성 및 암호화 식별 도구 개발)

  • 김윤희;이계상
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.28 no.6C
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    • pp.659-667
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    • 2003
  • IPsec refers to a standardized set of security protocols and algorithms which can provide the integrity, the authentication and the confidentiality services for IP packets in the Internet. Between two security gateways, IPsec provides the access control, the connectionless Integrity, data origin authentication, the anti-replay, and the confidentiality services, not only to the IP layer but also to the upper layers. In this paper, we describe a VPN (Virtual Private Network) testbed configuration using the FreeS/WAN and analyze the ISAKMP messages exchanged between the linux security gateway during the IKE SA setup. Also, we describe our development of an IPSEC encryption verification tool which can be used conveniently by VPN administrators.

A Probabilistic Seamless Communication Method to Provide Multimedia Services in Mobile Networks (이동 네트워크에서 멀티미디어 서비스 제공을 위한 확률적 무단절 통신 방법)

  • Kim, Yoon-Jeong;Bae, Ihn-Han
    • The Transactions of the Korea Information Processing Society
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    • v.7 no.2
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    • pp.446-453
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    • 2000
  • Mobile computing refers to an emerging new computing environment incorporating both wireless and wired high-speed networking technologies. In the near future, it is expected that mobile users will have access to a wide variety of services that will be made available over highspeed networks. The quality of these services in the high-speed networks can be specified in terms of several QoS paameters. The important QoS parameter in mobile computings is the guarantee for seamless communication which is to provide disruption free service to mobile users. A disruption in service could occur due to active handoffs. This paper proposes an extended staggened muticast approach which provides a probabilistic guarantee for disruption free service. The extended staggerd multicast approach estimates mobility direction and mobility velocity for a user. It is possible that data packets for a mobile host are multicasted to not all neighbor cells but a part of neighbor cells on the basis of these information. Therefore, the extended staggered multicast significantly reduces the static network bandwidth usage also provides a probabilistic guarantee for disruption free service.

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Performance Evaluation of OFDM-based IEEE 802.lla MAC Protocol Under Indoor Wireless Channel

  • Kim, Kanghee;Seokjo Shin;Kim, Kiseon
    • Proceedings of the IEEK Conference
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    • 2000.07b
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    • pp.739-742
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    • 2000
  • In this paper, we evaluate the throughput and delay performance of a wireless Local Area Network(WLAN) employing the OFDM-based IEEE 802.lla Medium Access Control(MAC) protocol by compute. simulations under wireless indoor. channel. Packet Error Rate(PER) is also investigated for the various Eb/No. It is shown that, with soft-decision Viterbi decoder, throughput and delay performance are close to those of error-free channel at Eb/No above 8dB and PER is about 2${\times}$10$\^$-5/ at Eb/No=10dB.

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Integrated Management Middleware for the Servers supporting the terminal-independent (단말기 독립형 서버 통합 관리 미들웨어)

  • Kim Jeong-Hee;Kwak Ho-Young
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.10 no.6
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    • pp.1106-1118
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    • 2006
  • The Internet has become an important instrument for providing many pieces of information to us. And server systems offer the various services after processing a volume of information, it's network topology also has been complex for providing information. Therefore this expansion means the importance of fast and sustained management of server systems. But most of server systems are located in fire-walls to allow only restricted access. Moreover, due to the difference of management based on the different operating system of each server system, management of server system is not to efficiency. That is, sever administrators have to manage by themselves many kinds of servers by using local-based or socket-based single access applications. Therefore, we design and implement a middleware system which allows to manage many kinds of severs integrally ruling XML-based Web Services which have distributed object technology such as CORBA, Java RMI and DCOM, but are free from platforms or development languages. System also provides location transparency and terminal independence and calls a server system management command to manage systems.

Trends in Digital Security Policies and Trade Rules in Major Overseas Countries (디지털 보안에 대한 해외 주요국의 통상 규범 동향)

  • J.E. Kim
    • Electronics and Telecommunications Trends
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    • v.38 no.4
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    • pp.1-11
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    • 2023
  • Trade rules in service and digital sectors mainly focus on reducing regulatory uncertainties by improving transparency and minimizing unnecessary requirements. Recognizing the importance of digital trade rules and trade in information and communication technology (ICT) sectors, governments worldwide have rapidly adopted and expanded rules on free flow of data, personal data protection, electronic authentication, and cybersecurity. On the other hand, advances in technology have led governments to face multiple threats related to cybersecurity, intellectual property (including that related to source code and algorithms), and unauthorized access to proprietary information of their suppliers. This study presents digital trade rules related to digital security emphasizing cybersecurity, source code, and ICT products that use cryptography in different trade agreements. Additionally, it introduces various approaches that major countries are taking to both address digital security issues and seek balance between security enhancement and trade liberalization.

APC: An Adaptive Page Prefetching Control Scheme in Virtual Memory System (APC: 가상 메모리 시스템에서 적응적 페이지 선반입 제어 기법)

  • Ahn, Woo-Hyun;Yang, Jong-Cheol;Oh, Jae-Won
    • Journal of KIISE:Computer Systems and Theory
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    • v.37 no.3
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    • pp.172-183
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    • 2010
  • Virtual memory systems (VM) reduce disk I/Os caused by page faults using page prefetching, which reads pages together with a desired page at a page fault in a single disk I/O. Operating systems including 4.4BSD attempt to prefetch as many pages as possible at a page fault regardless of page access patterns of applications. However, such an approach increases a disk access time taken to service a page fault when a high portion of the prefetched pages is not referenced. More seriously, the approach can cause the memory pollution, a problem that prefetched pages not to be accessed evict another pages that will be referenced soon. To solve these problems, we propose an adaptive page prefetching control scheme (APC), which periodically monitors access patterns of prefetched pages in a process unit. Such a pattern is represented as the ratio of referenced pages among prefetched ones before they are evicted from memory. Then APC uses the ratio to adjust the number of pages that 4.4BSD VM intends to prefetch at a page fault. Thus APC allows 4.4BSD VM to prefetch a proper number of pages to have a better effect on reducing disk I/Os, though page access patterns of an application vary in runtime. The experiment of our technique implemented in FreeBSD 6.2 shows that APC improves the execution times of SOR, SMM, and FFT benchmarks over 4.4BSD VM by up to 57%.

A Study on Public Property of the Regional Museum Design- Focused on the 21st Century Museum of Contemporary Art, Kanazawa, Ishikawaken in Japan - (지역 미술관디자인의 공공성에 관한 연구 - 일본 석천현 금택 21C 현대미술관을 대상으로 -)

  • Son Kwang-Ho;Kim Kang-Sub
    • Korean Institute of Interior Design Journal
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    • v.14 no.6 s.53
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    • pp.177-184
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    • 2005
  • The 21st Century Museum of Contemporary Art, Kanazawa as a regional public culture facility is the place where it is possible to think over the roles and meanings of a museum. The urn of this research is to provide information for exploring a desirable direction of the design of a regional museum by analyzing the architectural meanings and public property of the 21st Century Museum of Contemporary Art, Kanazawa. In addition, we arranged the totes and meanings of a regional museum and the concept of the public property in architecture. We also analyzed the architectural quality and the expression of the public property in this museum, as well as the value and meaning of a museum through field researches. The results of this research are as follows. First, this museum breaks with the existing stereotyped Idea to be a space open to the citizens. It secures a wide public space and provides the convenience of access and every facility for citizens. Second, the floor plan is divided into the interchanging area for citizens and an exhibition area; the outskirts of the inside of the museum are planned to be used free of charge for an interchanging space of citizens as a public-owned space of citizens. Third, the public-owned space of citizens includes a citizen exhibition hall, an art library, a kids studio and rest space, which ate all designed lot everyone to enjoy freely. Last, the exterior shape of the museum is simple but harmonize with the surroundings. It has an unique shape showing the local identity, and its most special properties are the convenience of access, introduction of a free space inside, and security of a huge space for children and citizens.

A Study on the Current Status of the Adoption of Creative Commons Licenses in Korean Citation Index Journals (KCI 등재지의 CCL 적용 현황 연구)

  • Kyoung Hee Joung
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.34 no.2
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    • pp.233-249
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    • 2023
  • This study aimed to understand the application and types of Creative Commons Licenses (CCL) in Korean journals and to increase understanding of the state of open access in South Korea. The study collected and analyzed CCL-related information from the websites of 2,097 KCI journals and compared the findings with DOAJ journals and previous research. The results of the study are summarized as follows. Firstly, 18% (377 journals) of the KCI journals adopted CCL, and among them, approximately 90% (337 journals) were in the field of STEM. There were few academic journals applying CCL in humanities and social sciences fields. Secondly, the types of CCL adopted by KCI journals were NC licenses (90%), with CC BY-NC (85%) being the most common. This is a significant difference from DOAJ journals, where 52% of academic journals applied CC BY or CC BY-SA. However, fewer journals applied licenses of the ND licenses than DOAJ journals and Spanish journals. Thirdly, 44% (167) of the 377 academic journals that applied CCL were simultaneously for free in public databases and sold for a fee in commercial databases. 33% (125) were publicly available for free in public databases, and 9% (33) were sold in commercial databases.

A Study on the Development of CITIS System Prototype - Using a Web Development Tool - (CITIS 시스템의 프로토타입 개발 방안 - 웹 개발 도구 사용을 중심으로 -)

  • 박정선;김성희
    • The Journal of Society for e-Business Studies
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    • v.2 no.1
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    • pp.117-129
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    • 1997
  • As the lifecycle of a product becomes shorter, the communication between a contractor and a supplier in product information has got more importance. The CITIS(Contractor Integrated Technical Information Service) is required to provide authorized users with access to both data and applications they need to complete a task for contract. With the growth of WWW, there has been much study for the use of WWW for the implementation of CALS. The WWW has been proved as an efficient tool for CALS implementation due to its of publicity, openness, and free accessibility. In this study, we suggest using WWW tools, here, an intranet tool, as a basis for the implementation of CITIS

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