• Microbiology and Biotechnology Letters
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• v.44 no.3
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• pp.355-362
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• 2016

The phosphomannomutase/phosphoglucomutase gene (pmm/pgm) of Vibrio anguillarum (the causative agent of fish vibriosis) was cloned, and the open reading frame corresponded to a protein with 446 amino acids. The pmm/pgm gene showed a significant degree of sequence homology with the previously reported genes from V. mimicus, V. vulnificus, V. splendidus, and V. harveyi, with 92.3%, 91.4%, 89.9%, and 89.9% amino acid identity, respectively. By reverse transcriptase-polymerase chain reaction, we found that the pmm/pgm gene was upregulated under cold stress condition. The PMM/PGM protein is known to catalyze the interconversion between mannose-1-phosphate and mannose-6-phosphate or glucose-1-phosphate and glucose-6-phosphate, which are important intermediates for lipopolysaccharide (LPS) biosynthesis. To confirm the role of PMM/PGM in the LPS biosynthetic pathway, we constructed a knock out mutant by homologous recombination. The respective LPSs were isolated from the V. anguillarum wild-type and mutant strains, and changes were compared by subjecting them to sodium dodecyl sulfate polyacrylamide gel electrophoresis. Based on the different patterns of the LPSs, we expect the pmm/pgm gene to have an important role in LPS biosynthesis. The pmm/pgm-deficient mutant of V. anguillarum will contribute to further studies about the role of LPS in V. anguillarum pathogenesis.

• Journal of Broadcast Engineering
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• v.8 no.4
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• pp.325-338
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• 2003

Video adaptation in response to dynamic resource conditions and user preferences is required as a key technology to enable universal multimedia access (UMA) through heterogeneous networks by a multitude of devices In a seamless way. Although many adaptation techniques exist, selections of appropriate adaptations among multiple choices that would satisfy given constraints are often ad hoc. To provide a systematic solution, we present a general conceptual framework to model video entity, adaptation, resource, utility, and relations among them. It allows for formulation of various adaptation problems as resource-constrained utility maximization. We apply the framework to a practical case of dynamic bit rate adaptation of MPEG-4 video streams by employing combination of frame dropping and DCT coefficient dropping. Furthermore, we present a descriptor, which has been accepted as a part of MPEG-21 Digital Item Adaptation (DIA), for supporting terminal and network quality of service (QoS) in an interoperable manner. Experiments are presented to demonstrate the feasibility of the presented framework using the descriptor.

• Journal of Korean Society of Steel Construction
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• v.18 no.1
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• pp.11-22
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• 2006

Message Signs (VMS) structures offer an increase in traffic safety through their ability to relay massages to motorists for warnings of hazards ahead, traffic congestion, accidents, and lane closings. The geometry of these signs sometimes results in the significant cyclic loading of the supports structure due to wind gusts, which can result from passing trucks or from natural wind. This study presents the results of analytical and experimental investigations of VMS structures. The commercially available softwareGTSTRUDL (2003) was used to perform space-frame structural analyses of these welded tubular structures. Fatigue evaluations were performed using stress ranges from field measurements and from structural analyses. Based on the results of the structural analyses that were conducted, where fatigue design loadings that had been derived from AASHTO Specifications for Structural Supports for Highway Signs, Luminaries, and Traffic Signals (2001) were used, the structures that had been studied were found not to have infinite fatigue life. According to the limited measurements that were made in this study, the fatigue design loadings derived from AASHTO Specifications (2001) appear to be conservative, but they are not overly conservative. The results of this study should be used to make a reasonable design of VMS structures, and to maintain their standards.

• Journal of the Korea Concrete Institute
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• v.21 no.3
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• pp.327-335
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• 2009

In the seismic region, non-ductile structures often form soft story and exhibit brittle collapse. However, structure demolition and new structure construction strategies have serious problems, as construction waste, environmental pollution and popular complain. And these methods can be uneconomical. Therefore, to satisfy seismic performance, so many seismic retrofit methods have been investigated. There are some retrofit methods as infill walls, steel brace, continuous walls, buttress, wing walls, jacketing of column or beam. Among them, the infilled frames exhibit complex behavior as follows: flexible frames experiment large deflection and rotations at the joints, and infilled shear walls fail mainly in shear at relatively small displacements. Therefore, the combined action of the composite system differs significantly from that of the frame or wall alone. Purpose of research is evaluation on the seismic performance of infill walls, and improvement concept of this paper is use of SHCCs (strain-hardening cementitious composites) to absorb damage energy effectively. The experimental investigation consisted of cyclic loading tests on 1/3-scale models of infill walls. The experimental results, as expected, show that the multiple crack pattern, strength, and energy dissipation capacity are superior for SHCC infill wall due to bridging of fibers and stress redistribution in cement matrix.

• Journal of the Korean Wood Science and Technology
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• v.44 no.1
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• pp.30-39
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• 2016

The evaluation of the lateral load performance for larch glulam portal frames was carried out using glass fiber reinforced plastic (GFRP) as connector in two different systems: the GFRP-reinforced laminated plates combined with veneer, and GFRP rod joints glued with epoxy resins to replace usual metal connectors for the structural glulam rahmen joints. As a result the yield strength, ultimate strength, initial stiffness of glulams of GFRP rod joints glued with epoxy resin decreased to 49%, 52% and 61% compared to those of the conventional metal connector. This connector will be a stress device where the bonding strength between the GFRP rod and glued laminated timber is important. Thus, there will be a high possibility that a problem may occur when it is applied to the field. On the other hand, the GFRP-reinforced laminated plates and wood (Eucalyptus marginata) pin were measured all within 3% for all measurements of the yield strength, ultimate strength, initial strength and ductility factor, regardless of high cross sectional loss on the glued laminated timber slit joint. In addition, the variation of stiffness on the cycle was 35%, which was the lowest, confirming that it was almost the same performance as the specimen prepared with the metal connector.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.4 no.1
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• pp.27-48
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• 1984

This study is conserned with the optimization of reinforced concrete frames using limit state design theory. Formulations of the optimal design for reinforced concrete frame based on the limit state theory turn out to be the nonlinear programming problems which have to deal with the required steel area, the width and effective height of the beam and column section and the moment reduction factor as the design variables. The objective function is formulated as the total construction cost which considers the costs of steel, concrete and forming for the reinforced concrete frames, and the basic constraints are imposed upon both ultimate and serviciability limit state concepts. Also, the stress blocks assumpted in CP110 and Hognestad et al. theory are applied to analysis an ultimate resistant section force for the ultimate limit state and only the criteria of CP110 are used for serviciability limit state. The optimized technique which is applied to solve the nonlinear programming problems for the optimization of reinforced concrete frames is SUMT utilizing the modified Newton-Raphson method. This algorithm is used to test for the two reinforced concrete frames, and then is compared and analysized with the numerical results of reference(10) to examine its convergence, applicability and stability under the same conditions. The results of this study are discussed about the economy comparision of the optimal values for CP110 and Hognestad et al., and the applicability, stability, convergence and validity of this algorithm used herein through the numerical analyses.

• Molecules and Cells
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• v.25 no.1
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• pp.55-63
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• 2008

Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder characterized by the selective death of motor neurons. Mutations in the SOD1 gene are responsible for a familial form of ALS (FALS). Although many studies suggest that mutant SOD1 proteins are cytotoxic, the mechanism is not fully understood. To investigate the role of mutant SOD1 in FALS, human SOD1 genes were fused with a PEP-1 peptide in a bacterial expression vector to produce in-frame PEP-1-SOD fusion proteins (wild type and mutants). The expressed and purified PEP-1-SOD fusion proteins were efficiently transduced into neuronal cells. Neurones harboring the A4V, G93A, G85R, and D90A mutants of PEP-1-SOD were more vulnerable to oxidative stress induced by paraquat than those harboring wild-type proteins. Moreover, neurones harboring the mutant SOD proteins had lower heat shock protein (Hsp) expression levels than those harboring wild-type SOD. The effects of the transduced SOD1 fusion proteins may provide an explanation for the association of SOD1 with FALS, and Hsps could be candidate agents for the treatment of ALS.

• Journal of Korean Society of Steel Construction
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• v.28 no.5
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• pp.345-354
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• 2016

In the present study, hybrid moment connections of welding and bar reinforcement for composite beam-column connections were proposed. Concrete-filled octagonal tube and U-section were used for the column and beam, respectively. In the beam-column connection, the top flange and web of the beam U-section were connected to the column plate by welding. However, to reduce stress concentration at the weld joints, the bottom flange of the beam was not welded to the column plate. Instead, to transfer the tension force of the beam flange, reinforcing bars passing through the column plate were used. Four exterior connections with conventional welded and hybrid moment connections were tested under cyclic loading and their cyclic behaviors were investigated. The test results showed that the hybrid moment connections successfully transferred the beam moment to the column. The strength and ductility of the hybrid moment connections were comparable to the conventional welded moment connection with exterior diaphragm; however, the connection performance was significantly affected by the details of the hybrid moment connection.

• Journal of Korean Society of Steel Construction
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• v.29 no.2
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• pp.181-192
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• 2017

A beam splice method using inclined end-plates and high-strength tension bolts was developed. The end-plates welded to a bracket and a spliced beam are connected each other by using the tension bolts. In the present study, six exterior beam-to-column moment connections were tested under cyclic loading. Test parameters were the end-plate details and bolt arrangements. All specimens were designed so that moment resistances of the end-plates and bolts were greater than the required moment at the beam splice, in accordance with the design methods of AISC Design Guide 4. Test results showed that in the beam splices with the extended end-plates, the beam moment successfully transferred to the bracket, without any defeats such as excessive prying action of the end plates and brittle failure at the end plate-to-beam flange weld joints. However, the deformation capacities of the overall beam-to-column connections were limited due to the brittle failure of the beam-to-column flange weld joints. From the test results, recommendations for seismic design and detailing of the beam-to-column moment connection with inclined end-plate beam splice were given.

• Journal of Navigation and Port Research
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• v.30 no.6 s.112
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• pp.439-446
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• 2006

In ship structures many of the structural plates have cutouts, for example, at inner bottom structure, girder, upper deck hatch, floor and dia-frame etc. In the case where a plate has a cutout it experiences reduced buckling and ultimate strength and at the same time the in-plane stress under compressive load produced by hull girder bending will be redistributed In general, actual ship structure adopted reinforcement of stiffener around the cutout in order to preventing from buckling so it need to examine a buckling and ultimate strength behaviour considering a cutout because In many ship yards used class rule for calculating buckling strength but it is difficult to evaluate perforate stiffened plate with random size. In the present paper, we investigated several kinds of perforated stiffened model from actual ship and then was performed finite element series analysis varying the cutout ratio, web height, thickness and type of cross-section using commercial FEA program(ANSYS) under compressive load.