• Journal of Ginseng Research
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• 제33권4호
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• pp.249-255
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• 2009

The oxidation of glycolate to glyoxylate, a key step in plant photorespiration, is carried out by the peroxisomal flavoprotein glycolate oxidase (EC 1.1.3.15). To investigate the altered gene expression and the role of GOX in ginseng plant defense system, a cDNA clone containing a GOX gene designated as PgGOX was isolated and sequenced from Panax ginseng. The cDNA was 692 nucleotides long and have an open reading frame of 552 bp with a deduced amino acid sequence of 183 residues. A GenBank BlastX search revealed that the deduced amino acid of PgGOX shares a high degree homology with the Glycine max (95% identity). In the present study we analyzed the expression of PgGOX under various environmental stresses at different times using real time-PCR. The results showed that the expressions of PgGOX increased after various treatments involving salt, light, cold, ABA, SA, and copper treatment.

• 한국전산구조공학회논문집
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• 제25권1호
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• pp.19-26
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• 2012

본 논문에서는 강판벽이 설치된 골조 구조물의 연쇄붕괴 거동을 비선형 정적 pushdown 해석을 이용하여 평가하였다. 해석모델은 중력하중에 대해서 설계된 2층 2경간 철골구조물이며, 중앙 기둥을 제거하고 하중을 서서히 증가시키며 하중-변위 관계를 구하였다. 구조물의 전체적인 거동뿐만 아니라 부분적인 응력과 변형을 파악하기 위하여 ABAQUS를 이용한 유한요소해석을 수행하였다. 해석을 통해서 구조물의 경간 길이 및 설치된 강판의 두께의 변화에 따른 연쇄붕괴 거동을 평가하였으며, 샛기둥을 이용하여 강판을 분할하고 분할된 강판의 위치에 따른 연쇄붕괴 성능의 변화를 관찰하였다. 해석결과에 따르면 경간의 길이가 증가할수록 연쇄붕괴를 방지하기 위하여 요구되는 강판의 두께 또한 증가하며, 분할된 강판의 수가 증가할수록 연쇄붕괴에 대한 저항성능이 약간 증가하지만 그 영향은 그리 크지 않은 것으로 나타났다. 또한 개구부로 인하여 일부 경간에만 강판이 설치된 경우에도 연쇄붕괴 저항성능이 어느 정도 증가하는 것으로 나타났다.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.50-50
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• 2003

We have identified and analysed a putative response regulator two-component gene (CaSKN7) from Candida albicans and its encoding protein (CaSkn7). CaSKN7 has an open reading frame of 1677bp. CaSKN7 encodes a 559 amino acid protein (CaSkn7) with an estimated molecular mass of 61.1 kDa. CaSKN7 is a homologue of a Saccharomyces cerevisiae SKN7 that is the regulator involved in the oxidative stress response. To study the role of CaSKN7, we constructed a CAI4-derived mutant strain carrying a homozygous deletion of the CaSKN7 gene. In the caskn7 disruptant cells, the formation of germ tube require shorter time than that in the congenic wild-type strain but the growth of mycelium delayed in liquid media. In contrast, the caskn7 disruptant cells attenuate the differentiation in solid media and the virulence in mouse model system. Expression level of hypha-specific and virulence genes - HYR1, ECE1, HWP1, and ALS1 - in the caskn7 disruptant cells increased as compared with that in the congenic wild-type strain in 10％ serum YPD. Skn7 in 5. cerevisiae was found to bind the HSE element from the SSA promoter, Also, CaSkn7 contains heat shock factor DNA-binding domain and the promoters of these genes have HSE-like sties. Therefore these results show that CaSKN7 regulate the differentiation and virulence of C. albicans.

• Journal of Ginseng Research
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• 제34권1호
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• pp.17-22
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• 2010

Sesquiterpenes are found naturally in plants and insects as defensive agents or pheromones. They are produced in the cytosolic acetate/mevalonate pathway for isoprenoid biosynthesis. The inducible sesquiterpene synthases (STS), which are responsible for the transformation of the precursor farnesyl diphosphate, appear to generate very few olefinic products that are converted to biologically active metabolites. In this study, we isolated the STS gene from Panax ginseng C.A. Meyer, designated PgSTS, and investigated the correlation between its expression and various abiotic stresses using real-time PCR. PgSTS cDNA was observed to be 1,883 nucleotides long with an open reading frame of 1,707 bp, encoding a protein of 568 amino acids. The molecular mass of the mature protein was determined to be 65.5 kDa, with a predicted isoelectric point of 5.98. A GenBank BlastX search revealed the deduced amino acid sequence of PgSTS to be homologous to STS from other plants, with the highest similarity to an STS from Lycopersicon hirsutum (55% identity, 51% similarity). Real-time PCR analysis showed that different abiotic stresses triggered significant induction of PgSTS expression at different time points.

• Child Health Nursing Research
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• 제8권3호
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• pp.322-333
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• 2002

Purpose: The purpose of study was : 1) to analyse the trend of research on the sensory stimulation in newborn in Korea, 2) to suggest direction for further study on the sensory stimulation in newborn and contributing to the use of intervention in newborn. Method: Research studies on the sensory stimulation in newborn were selected from the dissertations, parents-child health. Academic Society Journal, and from Korean Journal of Pediatrcs which were conducted between 1984 and 2002. The total numbers of the studies were 15. These studies were analyzed for 1) time of publication or presentation 2) thesis for a degree or nondegree 3) characteristics of subjects 4) sample size 5) research design 6) type of sensory stimualtion 7) meaurement tool 8) result. Result : 1)The most year of studies were the 1996's～ 2000's. 2) The degree of studies were higher than nondegree. 3) The most characteristics of subjects were the premature infants. 4) The most frequent sample sizes were 11-20. 5) The all research design was nonequivalent control group pretest-posttest design in quasi experimental basis. 6) The most type of sensory stimulation was Field massage(tactile and kinesthetic). 7) The measurement tool used all objective tools. 8) The most research data were collected by the measure of the weight, heart rate, behavior state, respiration, stress hormon and positive results. Conclusion: 1) Human sensory stimulation did not seem to harm the infants. 2) several positive effects of sensory stimulation that have been reported previously must be reevaluated. 3) further research will be required large sample size, long-term outcome, individualized stimulation program, early parent-infant contact in order to enhance the development of attachment, to determine the underlying mechanism and theorical frame for these stimulation effect.

• 한국공간구조학회논문집
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• 제16권2호
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• pp.61-68
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• 2016

Behavior of RC(Reinforced-concrete) beam-column connections has been subjected to the earthquake loading has been determined by shear and attachment mechanism. However, since the shear and attachment are very fragile for cycle loadings. Through occurring plastic hinges at the beam, the column and the connection should remain elastic condition and the beam should dissipate the energy from the earthquake. This study was investigate on the seismic performance of 6 RC beam - column connections built with the high strength reinforcements (700MPa) based on design and detailing requirements in the ACI 318-05 Provision and KCI-07 appendix II. This is aimed to evaluate the effect of the high-strength reinforcements as used the beam-column connection members. The main comparisons were the seismic performance of the connections affect the seismic performance in terms of strength, stiffness and ductility, joint shear stress-strain. A total of 6 beam-column specimens were built with a 1/2 scale and subjected to the cyclic loadings. Main design considerations were the area of the longitudinal reinforcements of the beam and details of the beam-column joint designed based on the seismic code. Cyclic test results are given and recommendations for the usage of high strength reinforcements for the seismic design is provided.

• 전기학회논문지
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• 제65권3호
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• pp.411-418
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• 2016

In accordance with global energy conservation policies such as MEPS(Minimum Energy Performance Standard), electric motor industry is moving to super-high-efficiency machines and research to develop IE4 (International Energy Efficiency Class4) motors has been launched. In this situation, SynRM (Synchronous Reluctance Motor) has been attracting attention in place of induction motor which hardly provides super premium efficiency. As a result, much research on SynRM is being performed at home and abroad. Also, some products have already been appearing in the market. Compared to induction motor, SynRM has better efficiency per unit area and wider operating range. Although the utilization of control system in synchronous motor results in higher prices, we still need to concentrate on developments of SynRM so as to comply with the new policies. This study demonstrated the electromagnetic design methods of super-premium SynRM while maintaining the frame of existing IE3 induction motor for blower. We documented the design procedures for generating high saliency which is the most essential and mechanical stress analysis is also treated. In conclusion, we proved the validity of our design by manufacturing and testing our SynRM models.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2017년도 춘계학술대회
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• pp.205-206
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• 2017

2016년 7000만 달러를 투자해 스마트폰 기반 캐주얼 게임을 각색한 3D 애니메이션 영화 <앵그리버드>가 상영된 후 전 세계적으로 누적 흥행 수입 5.14억 달러를 초과하는 등 큰 성공을 이루었다. 게임을 각색한 영화에서 입소문과 흥행 성적이 모두 좋았던 성공 사례는 매우 드물다. 구체적인 이야기 줄거리, 캐릭터 그리고 세계관이 없는 스마트폰 기반 캐쥬얼 게임을 어떻게 대중들의 사랑을 한 몸에 받는 상업 영화로 각색할 수 있었는지가 본 연구 목적이다. 본 연구는 먼저 스마트폰 기반 캐쥬얼 게임 <앵그리 버드>와 대형 온라인 게임 <와우>에 대해 비교를 진행하여 각색함에 있어서 장단점을 비교하고자 한다. 다음 <앵그리 버드>의 게임과 영화를 비교하여 두 매체 속에 존재하는 핵심요인 간 차이를 분석하고 설문조사를 통한 관객 평가 결과를 참조하여 가장 관객들의 인정을 받는 영화의 요소와 이런 종류의 영화에 대한 관객의 태도는 어떠한지를 알아보고자 한다.

• 건축역사연구
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• 제23권1호
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• pp.7-19
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• 2014

This study considers the proper repair techniques by examining the most representative repair cases of the Korean arch bridges and proposes the constructional manual which can apply similar occasions. The cases are Seonamsa Seungseongyo and Songgwangsa Geukrockgyo where this researcher had taken part in the repair works. This Study proposes the maintenance construction manual about the performance degradation drew by performance degradation of the both Korean arch bridges in the maintenance process. First, arch bridge maintenance should be carried out in the dry season, when water is impermeable in the bottom surface of the bridge. Moreover, risk factors of the maintenance should be excluded to secure the water vally flow, the bypass and the temporary bridge. Second, prior to repair, it has to precede (1)3D shooting (2)formal examination (3)structure safety test (4)geological and lithic surveys (5)arch curvature establishment and makeshift frame settlement before transformation (6)relationship expert comments. Third, if the baduk and the foundation stones are inevitable to replace due to performance degradation on the foundation, it should use the high quality stones and secure greater stress by extending the standard range. The foundation on irregular rock needs to be flattened and underside on the replaced materials require Grengyijil to deliver the equal loads. Fourth, In the process of dismantling the stones of the arched bridge, it could make heavy weathering degree and not reuse the materials. Charge should converge the expert advices to choose the reuseable, the conservate and the alternative materials, and increase the reutilization of the raw materials by preservation and reinforcement treatments. Fifth, the side wall should be repaired by the rubble work technique which is not able to pile compost satiety, so it must use long depth of masonary stones for reinforcement. It is considered to reinforce the stone wall in shore as much as possible and protect the abutment and the side wall on the upstream for the arch bridge maintenance works.

• Journal of Ginseng Research
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• 제44권1호
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• pp.79-85
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• 2020

Background: Helicobacter pylori increases reactive oxygen species (ROS) and induces oxidative DNA damage and apoptosis in gastric epithelial cells. DNA damage activates DNA damage response (DDR) which includes ataxia-telangiectasia-mutated (ATM) activation. ATM increases alternative reading frame (ARF) but decreases mouse double minute 2 (Mdm2). Because p53 interacts with Mdm2, H. pylori-induced loss of Mdm2 stabilizes p53 and induces apoptosis. Previous study showed that Korean Red Ginseng extract (KRG) reduces ROS and prevents cell death in H. pylori-infected gastric epithelial cells. Methods: We determined whether KRG inhibits apoptosis by suppressing DDRs and apoptotic indices in H. pylori-infected gastric epithelial AGS cells. The infected cells were treated with or without KRG or an ATM kinase inhibitor KU-55933. ROS levels, apoptotic indices (cell death, DNA fragmentation, Bax/Bcl-2 ratio, caspase-3 activity) and DDRs (activation and levels of ATM, checkpoint kinase 2, Mdm2, ARF, and p53) were determined. Results: H. pylori induced apoptosis by increasing apoptotic indices and ROS levels. H. pylori activated DDRs (increased p-ATM, p-checkpoint kinase 2, ARF, p-p53, and p53, but decreased Mdm2) in gastric epithelial cells. KRG reduced ROS and inhibited increase in apoptotic indices and DDRs in H. pylori-infected gastric epithelial cells. KU-55933 suppressed DDRs and apoptosis in H. pylori-infected gastric epithelial cells, similar to KRG. Conclusion: KRG suppressed ATM-mediated DDRs and apoptosis by reducing ROS in H. pylori-infected gastric epithelial cells. Supplementation with KRG may prevent the oxidative stress-mediated gastric impairment associated with H. pylori infection.