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http://dx.doi.org/10.5142/JGR.2010.34.1.017

Isolation of Sesquiterpene Synthase Homolog from Panax ginseng C.A. Meyer  

Khorolragchaa, Altanzul (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Parvin, Shohana (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Shim, Ju-Sun (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Kim, Yu-Jin (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Lee, Ok-Ran (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
In, Jun-Gyo (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Kim, Yeon-Ju (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Kim, Se-Young (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Yang, Deok-Chun (Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University)
Publication Information
Journal of Ginseng Research / v.34, no.1, 2010 , pp. 17-22 More about this Journal
Abstract
Sesquiterpenes are found naturally in plants and insects as defensive agents or pheromones. They are produced in the cytosolic acetate/mevalonate pathway for isoprenoid biosynthesis. The inducible sesquiterpene synthases (STS), which are responsible for the transformation of the precursor farnesyl diphosphate, appear to generate very few olefinic products that are converted to biologically active metabolites. In this study, we isolated the STS gene from Panax ginseng C.A. Meyer, designated PgSTS, and investigated the correlation between its expression and various abiotic stresses using real-time PCR. PgSTS cDNA was observed to be 1,883 nucleotides long with an open reading frame of 1,707 bp, encoding a protein of 568 amino acids. The molecular mass of the mature protein was determined to be 65.5 kDa, with a predicted isoelectric point of 5.98. A GenBank BlastX search revealed the deduced amino acid sequence of PgSTS to be homologous to STS from other plants, with the highest similarity to an STS from Lycopersicon hirsutum (55% identity, 51% similarity). Real-time PCR analysis showed that different abiotic stresses triggered significant induction of PgSTS expression at different time points.
Keywords
Panax ginseng; sesquiterpene synthase (STS); abiotic stress; real-time PCR;
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1 Back K, Chappell J. Cloning and bacterial expression of a sesquiterpene cyclase from Hyoscyamus muticus and its molecular comparison to related terpene cyclases. J Biol Chem. 270: 7375-7381 (1995)   DOI   ScienceOn
2 Yoshioka H, Yamada N, Doke N. cDNA cloning of sesquiterpene cyclase and squalene synthase, and expression of the genes in potato tuber infected with Phytophthora infestans. Plant Cell Physiol. 40: 993-998 (1999)   DOI   ScienceOn
3 Colby SM, Crock J, Dowdle-Rizzo B, Lemaux PG, Croteau R. Germacrene C synthase from Lycopersicon esculentum cv. VFNT Cherry tomato: cDNA isolation, characterization, and bacterial expression of the multiple product sesquiterpene cyclase. Proc Natl Acad Sci. 95: 2216-2221 (1998)   DOI
4 Chang YJ, Song SH, Park SH, Kim SU. Amorpha-4,11-diene synthase of Artemisia annua: cDNA isolation and bacterial expression of a terpene synthase involved in artemisinin biosynthesis. Arch Biochem Biophys. 383: 178-184 (2000)   DOI   ScienceOn
5 Kumar S, Tamura K, Nei M. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief in Bioinform. 5: 150-163 (2004)   DOI   ScienceOn
6 Schenk PM, Kazan K, Wilson I, Anderson JP, Richmond T, Somerville SC, Manners JM. Coordinated plant defense responses in Arabidopsis revealed by microarray analysis. PNAS 97: 11655-11660 (2000)   DOI   ScienceOn
7 Faldt J, Arimura G, Gershenzon J, Takabayashi J, Bohlmann J. Functional identification of AtTPS03 as (E)-${\beta}$-ocimene synthase: a monoterpene synthase catalyzing jasmonate and wound induced volatile formation in Arabidopsis thaliana. Planta 216: 745-751 (2003)
8 Smet ID, Zhang H, Inz D, Beeckman T. A novel role for abscisic acid emerges from underground. Trends in Plant Sci. 11: 434-439 (2006)   DOI   ScienceOn
9 Picaud S, Olofsson L, Brodelius M, Brodelius PE. Expression, purification, and characterization of recombinant amorpha- 4,11-diene synthase from Artemisia annua L. Arch Biochem Biophys. 436: 215-226 (2005)   DOI   ScienceOn
10 Chen XY, Chen Y, Heinstein P, Davisson VJ. Cloning, expression, and characterization of (+)-${\delta}$-cadinene synthase: a catalyst for cotton phytoalexin biosynthesis. Arch Biochem Biophys. 324: 255-266 (1995)   DOI   ScienceOn
11 Murashige T, Skoog F. A revised medium for rapid growth and bio assay with tobacco tissue cultures. Plant Physiol. 15: 473-499 (1962)   DOI
12 Facchini PJ, Chappell J. Gene family for an elicitor-induced sesquiterpene cyclase in tobacco. Proc Natl Acad Sci. 89: 11088-11092 (1992)   DOI   ScienceOn
13 Back K, He S, Kim KU, Shin DH. Cloning and bacterial expression of sesquiterpene cyclase, a key branch point enzyme for the synthesis of sesquiterpenoid phytoalexin capsidiol in UV-challenged leaves of Capsicum annuum. Plant Cell Physiol. 39: 899-904 (1998)   DOI   ScienceOn
14 Kim MK, Lee BS, In JG, Sun H, Yoon JH, Yang DC. Comparative analysis of expressed sequence tags (ESTs) of ginseng leaf. Plant Cell Rep. 25: 599-606 (2006)   DOI
15 Morris PC, Kumar A, Bowles DJ, Cuming AC. Osmotic stress and abscisic acid induce expression of the wheat Em genes. Eur J Biochem. 190: 625-630 (1990)   DOI   ScienceOn
16 Jing F, Zhang L, Li M, Tang Y, Wang Y, Wang Y, Wang Q, Pan Q, Wang G, Tang K. Abscisic acid (ABA) treatment increases artemisinin content in Artemisia annua by enhancing the expression of genes in artemisinin biosynthetic pathway. Biologia 64: 319-323 (2009)   DOI   ScienceOn
17 Alschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402 (1997)   DOI
18 Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 25: 4876-4882 (1997)   DOI
19 Smith RJ, Hobson S, Ellis IR. The effect of abscisic acid on calcium-mediated regulation of heterocyst frequency and nitrogenase activity in Nostoc 6720. New Phytologist 105: 543-549 (1987)   DOI   ScienceOn
20 Gundlach H, Muller MJ, Kutchan TM, Zenk MH. Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. PNAS 89: 2389-2393 (1992)   DOI   ScienceOn
21 Cho CW, Lee HJ, Chung ES, Kim KM, Heo JE, Kim JI, Chung J, Ma Y, Lee DW, Kim DH, Chung YS, Lee JH. Molecular characterization of the soybean L-asparaginase gene induced by low temperature stress. Mol Cells 23: 280- 286 (2007)
22 Arimura GI, Garms S, Maffei M, Bossi S, Schulze B, Leither M, Mithofer A, Boland W. Herbivore-induced terpenoid emission in Medicago truncatula: concerted action of jasmonate, ethylene and calcium signaling. Planta 227: 453-464 (2008)   DOI
23 Yu F, Harada H, Yamasaki K, Okamoto S, Hirase S, Tanaka Y, Misawa N, Utsumi, R. Isolation and functional characterization of a ${\beta}$-eudesmol synthase, a new sesquiterpene synthase from Zingiber zerumbet Smith. FEBS Letters 582: 565-572 (2008)   DOI   ScienceOn
24 Pechous SW, Whitaker BD. Cloning and functional expression of an (E,E)-${\alpha}$-farnesene synthase cDNA from peel tissue of apple fruit. Planta 219: 84-94 (2004)   DOI   ScienceOn
25 Cheng AX, Xiang CY, Li JX, Yang CQ, Hu WL, Wang LJ, Lou YG, Chen XY. The rice (E)-${\beta}$-caryophyllene synthase (OsTPS3) accounts for the major inducible volatile sesquiterpenes. Phytochem. 68: 1632-1641 (2007)   DOI   ScienceOn