• 한국작물학회지
• /
• 제50권4호
• /
• pp.286-290
• /
• 2005

Ascorbate peroxidase (APX) plays a crucial role in the detoxification of hydrogen peroxide. APX activity is maintained significantly higher in the paraquat­treated leaves of the paraquat-tolerant Rehmannia glutinos. This study was conducted to understand structural and regulatory characteristics of APX gene in R. glutinosa. A putative APX cDNA clone (RgAPX1) was isolated from a leaf cDNA library using a partially sequenced expressed sequence tag clone. RgAPX1 is consisted of 1148 bp nucleotides and contains an open reading frame encoding a 250 amino acid-long polypeptide. Deduced RgAPX1 amino acid sequence shares higher sequence similarity to cytosolic APXs. RgAPX1. expression was higher in the leaf than in the flower and root. Southern blot result indicates the presence of one or two RgAPX1-related genes in R. glutinosa genome. RgAPX1 transcription was affected differentially by various stresses and phytohormone. The results indicate that RgAPXl is constitutively expressed in most tissues and its expression is modulated for the immediate and efficient detoxification of $H_2O_2$ under normal and stress conditions.

• 수산해양기술연구
• /
• 제35권1호
• /
• pp.54-59
• /
• 1999

High-speed electronic digital computers have enabled engineers to employ various numerical discretization techniques for solutions of complex problems. The Finite Element Method is one of the such technique. The Finite Element Method is one of the numerical analysis based on the concepts of fundamental mathematical approximation. Three dimensional plate structures used often in partition of ship, box girder and frame are analyzed by Finite Element Method. In design of structures, the static deflections, stress concentrations and dynamic deflections must be considered. However, these problem belong to geometrically nonlinear mechanical structure analysis. The analysis of each element is independent, but coupling occurs in assembly process of elements. So, to overcome such a difficulty the shell theory which includes transformation matrix and a fictitious rotational stiffness is taken into account. Also, the Mindlin's theory which is considered the effect of shear deformation is used. The Mindlin's theory is based on assumption that the normal to the midsurface before deformation is "not necessarily normal to the midsurface after deformation", and is more powerful than Kirchoff's theory in thick plate analysis. To ensure that a small number of element can represent a relatively complex form of the type which is liable to occur in real, rather than in academic problem, eight-node quadratic isoparametric elements are used. are used.

• Steel and Composite Structures
• /
• 제10권1호
• /
• pp.87-108
• /
• 2010

A Steel Plate Shear Wall (SPSW) is a lateral load resisting system consisting of an infill plate located within a frame. When buckling occurs in the infill plate of a SPSW, a diagonal tension field is formed through the plate. The study of the tension field behavior regarding the distribution and orientation patterns of principal stresses can be useful, for instance to modify the basic strip model to predict the behavior of SPSW more accurately. This paper investigates the influence of torsional and out-of-plane flexural rigidities of boundary members (i.e. beams and columns) on the buckling coefficient as well as on the distribution and orientation patterns of principal stresses associated with the buckling modes. The linear buckling equations in the sense of von-Karman have been solved in conjunction with various boundary conditions, by using the Ritz method. Also, in this research the effects of symmetric and anti-symmetric buckling modes and complete anchoring of the tension field due to lacking of in-plane bending of the beams as well as the aspect ratio of plate on the behavior of tension field and buckling coefficient have been studied.

• Fisheries and Aquatic Sciences
• /
• 제12권2호
• /
• pp.90-97
• /
• 2009

The cytoskeletal $\beta$-actin gene and its 5'-upstream region were isolated and characterized in the rockbream (Oplegnathus fasciatus). Complementary DNA of the rockbream $\beta$-actin represented a 1,125 bp of an open reading frame encoding 375 amino acids, and the rockbream $\beta$-actin cDNA and deduced amino acid sequences were highly homologous to those of other vertebrate orthologs. At the genomic level, the $\beta$-actin gene also exhibited an organization typical of vertebrate cytoskeletal actin genes (2,159 bp composed of five translated exons interrupted by four introns) with a conserved GT/AG exon-intron splicing rule. The putative non-translated exon predicted in the rockbream $\beta$-actin gene was much more homologous with those of teleostean $\beta$-actin genes than those of mammals. The 5'-upstream regulatory region isolated by genome walking displayed conserved and essential elements such as TATA, CArG and CAAT boxes in its proximal part, while several other immune- or stress-related motifs such as those for NF-kappa B, USF, HNF, AP-1 and C/EBP were in the distal part. Semi-quantitative RT-PCR assay results demonstrated that the rockbream $\beta$-actin transcripts were ubiquitously but different-tially expressed across the tissues of juveniles.

• Steel and Composite Structures
• /
• 제18권6호
• /
• pp.1405-1421
• /
• 2015

Semi-rigid connections are the actual behavior of beam-to-column connections in steel frames. However, the behavior of semi-rigid connections is not taken into account for the simplicity in the conventional analysis and design of steel frames. A computer-based analysis and design has been studied for the three-dimensional steel frames with semi-rigid connections. The nonlinear analysis which includes the effects of the flexibility of connections is used for this study. It is designed according to the buckling and combined stress constraints under the present loading after the joint deformations and the member end forces of the space frame are determined by the stiffness matrix method. The semi-rigid connection type is limited to the top and bottom angles with a double web angle connection. The Frye-Morris polynomial model is used to describe the non-linear behavior of semi-rigid connections. Various design examples are presented to demonstrate the efficiency of the method. The results of design and analysis of unbraced semi-rigid frames are compared to the results of unbraced rigid frames under the same design requirements.

• Earthquakes and Structures
• /
• 제5권5호
• /
• pp.527-552
• /
• 2013

Development of flexural yielding and large rotation ductilities in the plastic hinge zones of frame members is synonymous with the spread of bar reinforcement yielding into the supporting anchorage. Yield penetration where it occurs, destroys interfacial bond between bar and concrete and reduces the strain development capacity of the reinforcement. This affects the plastic rotation capacity of the member by increasing the contribution of bar pullout. A side effect is increased strains in the compression zone within the plastic hinge region, which may be critical in displacement-based detailing procedures that are linked to concrete strains (e.g. in structural walls). To quantify the effects of yield penetration from first principles, closed form solutions of the field equations of bond over the anchorage are derived, considering bond plastification, cover debonding after bar yielding and spread of inelasticity in the anchorage. Strain development capacity is shown to be a totally different entity from stress development capacity and, in the framework of performance based design, bar slip and the length of debonding are calculated as functions of the bar strain at the loaded-end, to be used in calculations of pullout rotation at monolithic member connections. Analytical results are explored parametrically to lead to design charts for practical use of the paper's findings but also to identify the implications of the phenomena studied on the detailing requirements in the plastic hinge regions of flexural members including post-earthquake retrofits.

• 제어로봇시스템학회:학술대회논문집
• /
• 제어로봇시스템학회 2005년도 ICCAS
• /
• pp.2307-2310
• /
• 2005

A pressurizer is one of the major safety related equipment of nuclear power plants. In order to inspect and maintain the internal structures of a pressurizer, jumpers have to enter the pressurizer, in spite of the high dose exposure. Therefore, a tele-operated robotic manipulator has been developed, which consists of four parts with 2DOFs arms, a gripper, base frame, and control console. The task of this robotic manipulator is to extract the damaged electric heaters and inspect the internal structure of the pressurizer. The gripper hanging from the manipulator approaches the heaters and extracts the damaged heater by using a self-locking mechanism. In order to investigate the structural stability of the robotic manipulator, a stress analysis has been performed by using the ANSYS code. The results of this paper include the position control and vibration control of robotic gripper and the development of processing visual information for a vision sensor.

• 한국지진공학회논문집
• /
• 제2권2호
• /
• pp.23-34
• /
• 1998

본 논문은 바닥판을 고려한 3차원 골조 구조물의 효율적인 연직 진동 해석법을 제안한다. 바닥판의 영향을 고려하면 구조물의 진동해석을 좀더 정확히 수행할 수 있는 반면, 면요소의 사용에 따른 많은 자유도의 사용으로 많은 해석시간과 기억용량이 필요하게 된다. 따라서, 행렬응축기법을 사용하여 적절한 요소분할 및 주자유도 선택 기법을 제안하였으며, 대형요소를 사용하여 정확화된 일반적인 건물의 동적해석에 적용하여 입력 데이터의 작성을 간단히 하고 동적해석을 빠르고 신뢰할 만한 결과를 얻을 수 있는 기법에 대하여 연구하였다.

• 국제초고층학회논문집
• /
• 제3권3호
• /
• pp.167-171
• /
• 2014

TORANOMON HILLS is the main building of a large-scale re-development project located in the center of Tokyo. This high-rise building has a height of 247 m and 52 floors above ground, 5 floors below ground, and $62m{\times}80m$ in plan. It is used as hotel, residential facilities, offices, shops and conference facilities. The super structure is mainly a rigid steel frame with response-control devices, using concrete-filled steel tube columns. The underground section is a mixed structure composed of steel, steel-reinforced concrete and reinforced concrete framings. The piled-raft foundation type is used. The remarkable feature of this high-rise building is that the motorway runs through the basements of the building, which makes it stand just above the motorway. This condition is an important factor of the building design. The plan shape is designed to fit along the curve of the motorway. Special columns at the corners are required to avoid placing columns in the motorway. This special column is a single inclined column in the lower floors that branches into two columns in the mid-floors to suit the column location in the upper floors. The cast steel joint is used for the branching point of each special column to securely transfer the stress.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 1998년도 하계학술대회 논문집 D
• /
• pp.1312-1314
• /
• 1998

SOFC system is often subject to thermal cycle condition during normal start/stop, shutdown, and emergence state. Under the thermal cycle condition of heating and cooling, the SOFC components expand or shrink, which produces thermal stress and thermal shock. The SOFC performance is degraded by the thermal factors. To protect SOFC system from the thermal degradation, the optimum thermal condition must be clarified. In this study, to examine the thermal cycle characteristics, we fabricated single cells of planar SOFC with an area of $5{\times}5cm$. The electrolyte and PEN were tested under thermal cycle conditions in the range of$ 2-8^{\circ}C/min$. After thermal cycle test. crack creation of the components were examined using ultraviolet apparatus. No crack in the electrolyte and PEN were observed. The single cell system with alumina frame were also tested under thermal cycle conditions of 2, 3, $4^{\circ}C/min$. The single cell was fractured at the thermal cycle of 3 and $4^{\circ}C/min$ and the optimum condition of the thermal cycle to be found below $2^{\circ}C/min$.