Browse > Article

Regulation of Ascorbate Peroxidase Gene Expression in Response to Stresses and Phytohormone in Rehmannia glutinosa  

Park Myoung Ryoul (Division of Biological Resources Sciences, Chonbuk Nat'l Univ.)
Park Moon Hee (Division of Biological Resources Sciences, Chonbuk Nat'l Univ.)
Yoo Nam Hee (Institute of Agricultural Science and Technology, Chonbuk Nat'l Univ.)
Yu Chang Yeon (Division of Applied Plant Sciences, Kangwon Nat'l Univ.)
Yun Song Joong (Division of Biological Resources Sciences, Chonbuk Nat'l Univ., Institute of Agricultural Science and Technology, Chonbuk Nat'l Univ.)
Publication Information
KOREAN JOURNAL OF CROP SCIENCE / v.50, no.4, 2005 , pp. 286-290 More about this Journal
Abstract
Ascorbate peroxidase (APX) plays a crucial role in the detoxification of hydrogen peroxide. APX activity is maintained significantly higher in the paraquat­treated leaves of the paraquat-tolerant Rehmannia glutinos. This study was conducted to understand structural and regulatory characteristics of APX gene in R. glutinosa. A putative APX cDNA clone (RgAPX1) was isolated from a leaf cDNA library using a partially sequenced expressed sequence tag clone. RgAPX1 is consisted of 1148 bp nucleotides and contains an open reading frame encoding a 250 amino acid-long polypeptide. Deduced RgAPX1 amino acid sequence shares higher sequence similarity to cytosolic APXs. RgAPX1. expression was higher in the leaf than in the flower and root. Southern blot result indicates the presence of one or two RgAPX1-related genes in R. glutinosa genome. RgAPX1 transcription was affected differentially by various stresses and phytohormone. The results indicate that RgAPXl is constitutively expressed in most tissues and its expression is modulated for the immediate and efficient detoxification of $H_2O_2$ under normal and stress conditions.
Keywords
ascorbate peroxidase (APX); oxidative stresses; paraquat; Rehmannia glutinosa;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Chung, I. M., M. R. Park, J. C Chun, and S. J. Yun. 2003 Resveratrol accumulation and resveratrol synthase gene expression in response to abiotic stresses and hormones in peanut plants, Plant Science. 164: 103-109   DOI   ScienceOn
2 Dodge, A. D. 1994. Herbicide action and effects on detoxification process. In Causes of Photo-oxidative Stress and Amelioration of Defense System in Plants (C.H. Foyer and P.M. Mullmeaux, Eds.), CRC Press, Boca Raton, FL, p.220-235
3 Sambrook, J. and D. W Russell. Molecular cloning: 2002 A laboratory manual, Cold Spring Harbor Laboratory Press, Cold Sprinng Harbor, NY
4 Shigeoka, S., T Ishikawa, M. Tamoi, Y Miyagawa, T. Takede, Y Yabuta, and K. Yoshmura, 2002. Regulation and function of ascorbate peroxidase isozymes. J. Exp. Bot 53 . 1305-1319   DOI   ScienceOn
5 Yu, Q., A. Cairns, and S B Powles 2004 Paraquat resistance in a population of Lolium rigidum. Func Plant Biol. 31 247-254   DOI   ScienceOn
6 Iannelli, M., F. van Breusegem, M. van Montagu, D Inze, and A. Massacci 1999. Tolerance to low temperature and paraquat-mediated oxidative stress in two maize genotypes J. Exp. Bot. 50 . 523-532   DOI   ScienceOn
7 Thomas, B. R. and D Pratt. 1982. Isolation of paraquat tolerant mutant from tomato cell culture, Theor. Appl Genet. 63 169-176   DOI   ScienceOn
8 Harper, D. B. and B. M. R. Harvey. 1987. Mechanism of paraquat tolerance in perennial rye grass II Role of superoxidase dismutase, catalase and perocidase, Plant Cell environ. 1: 211-215   DOI
9 Choi, D G, N H Yoo, C. H. Yu, B. D. L. Reyes, and S. J. Yun. 2004 The acitivities of antioxidant enzymes in response to oxidative stresses and hormones in paraquat-tolerant Rehmannia glutinosa plants. J Biochem. Mol. Biol. 37 . 618-624   DOI
10 Miyake, C., W H. Cao, and K. Asada. 1993. Purification and molecular properties of the thylacoid-bound ascorbate peroxidase in spinach chloroplasts. Plant Cell Physiol. 34 . 881-889
11 Cho, E. A., C. A Lee, Y. S Kim, S. H. Baek, B G. de los Reyes, and S. J. Yun. 2005. Expression of r-tocopherol methyltransferase transgene improves tocopherol composition in lettuce (Latuca sativa L.) Mol Cells 19 : 16-22
12 Mittler, Rand B. A. Zihnskas, 1991. Purification and characterization of a gene encoding pea cytosolic ascorbate peroxidase, Plant Physiol, 97 . 962-968   DOI   ScienceOn
13 Allen, R. D., R P. Webb, and S. A. Schake. 1997. Use of transgenic plants to study antioxidant defenses. Free Rad. Biol. Med. 23. 473-479   DOI   ScienceOn
14 Bunkelmann, J. R. and R. N. Trelease. 1996. Ascorbate peroxidase; a prominent membrane protein in oilseed glyoxisomes Plant Physiol. 110 . 589-598   DOI   ScienceOn
15 Kim, J. S. and J. C. Chun. 1992. Resistance of the medicinal plant jiwhang (Rehmannia glutinosa) to paraquat. Korean J. Weed Sci. 12: 374-378
16 Moon, Y. R., J. H. Lim, M. R. Park, C. Y Yu, I. M. Chung, D. C. Yang, and S. J Yun. 2004. Antioxidant enzyme responses against abiotic and biotic stresses in Rehmannia gluttinosa L. and Glycine max L Kor. J. Med. Crops 12: 360-365
17 Altschul, S F., T. L. Madden, A A. Schaffer, J. Zhang, Z. Zhang, M. Webb, and D. J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs Nucleic Acids Res. 25 3389-3402   DOI   ScienceOn
18 Rizhsky, L , E. Hallak-Herr, F. V. Breusegem, S. Rachmilevitch, J. E. Barr, S. Rodermel, D. Inze, and R. Mittler. 2002. Double antisense plants lacking ascorbate peroxidase and catalase are less sensitive to oxidative stress than single antisense plants lacking ascorbate peroxidase or catalase. Plant J. 32 : 329-342   DOI   ScienceOn
19 Chun, J. C., S Y Ma, S E. Kim, and H. J. Lee 1997. Physiological responses of Rehmannia glutinosa to paraquat and its tolerance mechanisms. Pestic, Biochem Physiol 59: 51-63   DOI   ScienceOn
20 Yoshimura, K. Y, Y. T Ishikawa, and S. Shigeoka. 2000. Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stresses. Plant Physiol, 123 : 223-234   DOI   ScienceOn
21 Kwon, S. Y, Y. J. Jeong, H S. Lee, J. S. Kim, K. Y Cho, R. D. Allen, and S. S. Kwak. 2003. Enhanced tolerance of transgenic tobacco plants expressing both superoxide dismutase and ascorbate peroxidase in chloroplast against methyl vilolgenmediated oxidative stress. Plant Cell Env. 25 . 873-882   DOI   ScienceOn
22 Shaaltiel, Y. and J. Gressel. 1986. Multienzyrne oxygen radical detoxification system correlated with paraquat resistance in Conyza banariens, Pest. Biochem, Physiol 26.22-28   DOI
23 Yoo, T. H., C J. Park, G J Lee, R Shin, J. H. Yun, K. J. Kim, K H. Rhee, and K. H. Paek. 2002. A hot pepper cDNA encoding ascorbate peroxidase is induced during the incompatible interaction with virus and bacteria Mol Cells 14: 75-84
24 Scandahos, J. G 1993. Oxygen stress and superoxide dismutase, Plant Physrol, 101 : 7-12   DOI
25 Donahue, J. L., C. M. Okpodu, C. I Cramer, E. A. Grabau, and R G Alscher 1997. Responses of antioxidants to paraquat in pea leaves Relationships to resistance, Plant Physiol, 113 249-257   DOI