• 미생물학회지
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• 제30권2호
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• pp.141-148
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• 1992

In order to overexpress bacteriophage PRD1 DNA polymerase in E. coli cells, the 2 kb HaeII fragment was isolated from phage genomic DNA. This fragment was then cloned into pEMBL/sup ex/ 3-expression vector. A specific 57bp deletion was performed by using uracil containing ss DNA and oligonucleotide spanning each region to remove an unwanted non-coding region. After this deletion, the PRD1 DNA polymerase gene is totally under the control of the vector promoter and SD sequence. Upon heat induction, a protein with an apparent size of 68 kdal was overexpressed as an active PRD1 DNA polymerase. The expression of PRD1 DNA polymerase was about 1% of total E. coli protein.

• Journal of Microbiology
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• 제37권2호
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• pp.51-58
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• 1999

Using yeast, Saccharomyces cerevisiae, and the integrate vector system, we have isolated and characterized an autonomously replicating sequence (ARS) from Aspergillus nidulans. The DNA fragment, designated ANR1, is 5.0 kb in size and maintained free from the chromosome in S. cerevisiae. The YIplac211-ANR1 recombinant plasmid, which consists of sequences derived from the yeast integrative vector YIplac211 and 5.0 kb ANR1 fragment, showed a 104-fold enhancement in transformation efficiency over that found for YIplac211, and was easily recovered from the transformed yeast. Genetic analysis of transformants showed that YIplac21-ANR1 could be over 96% cured when cultured over 20 generations in complete medium and thus suggests that this sequence is mitotically unstable. In A. nidulans, recombinant plasmid PILJ16-4.5 which carries the 4.5 kb EcoRI fragment of ANR1 showed a 170-fold enhancement in transformation efficiency compared to that of the integrative vector PILJ16.

• Interdisciplinary Bio Central
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• 제2권2호
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• pp.6.1-6.5
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• 2010

Fragment molecular orbital (FMO) method provides a novel tool for ab initio calculations of large biomolecules. This method overcomes the size limitation difficulties in conventional molecular orbital methods and has several advantages compared to classical force field approaches. While there are many features in this method, we here focus on explaining the issues related to protein-ligand binding: FMO method provides useful interaction-analysis tools such as IFIE, CAFI and FILM. FMO calculations can provide not only binding energies, which are well correlated with experimental binding affinity, but also QSAR descriptors. In addition, FMO-derived charges improve the descriptions of electrostatic properties and the correlations between docking scores and experimental binding affinities. These calculations can be performed by the ABINIT-MPX program and the calculation results can be visualized by its proper BioStation Viewer. The acceleration of FMO calculations on various computer facilities is ongoing, and we are also developing methods to deal with cytochrome P450, which belongs to the family of drug metabolic enzymes.

• Wind and Structures
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• 제17권2호
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• pp.185-202
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• 2013

The goal of this study was to investigate the vulnerability of roof tile systems and metal shutters to roof tile debris. Three phases addressed the performance of tile roof systems and metal shutters impacted by roof tile debris. The first phase experimentally evaluated the tile fragment size and quantity generated by a tile striking a tile roof system. The second phase experimentally quantified the puncture vulnerability of common metal panel shutter systems as a function of tile fragment impact speed. The third phase provided context for interpretation of the experimental results through the use of a tile trajectory model. The results provide supporting evidence that while metal panel window shutters provide significant protection against a prevalent form of windborne debris, these systems are vulnerable to tile fragment puncture in design level tropical cyclones. These findings correlate with field observations made after Hurricane Charley (2004).

• Archives of Pharmacal Research
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• 제15권1호
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• pp.58-61
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• 1992

Bacillus anthracis 590 having an inducibla resistance determinant to MLS antibiotics was isolated from a soli sample in Korea. The resistance gene (ernJ) was cloned by Southern blotting of chromosomal DNA fragment digested by various restriction enzymes and coloy hybridization method and the cloned plasmid was named as pBA423. The size of inserted DNA fragment of pBS42 vector was about 2.9 kb and the DNA sequence of the subcloned fragment (Hinc II-Hinc II, 1.4kb) WAS determined. The DNA sequence of ernJ was composed of 357 bp for leader region and 861 bp for the structural gene. Because the leader sequence of ernJ was homologous to that of ermK, the expression of ernJ is also thought to be controlled by a transcriptionl attenuation mechanism.

• 한국멀티미디어학회논문지
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• 제17권5호
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• pp.573-581
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• 2014

The salient features of UWB(Ultra WideBand) networks such as high-rate communications, low interference with other radio systems, and low power consumption bring many benefits to users, thus enabling several new applications such as wireless universal serial bus (WUSB) for connecting personal computers (PCs) to their peripherals and the consumer-electronics (CE) in people's living rooms. Because the size of multimedia data frame, WiMedia device must transmit the fragment of MSDU. However, when the fragment of MSDU is lost, WiMedia device maintains active mode for the time to complete the transmission MSDU, and there is a problem that unnecessary power consumption occurs. Therefore we propose new power management scheme to reduce unnecessary power consumption of WiMedia devices in the case that the fragment is lost.

• 대한바이러스학회지
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• 제26권2호
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• pp.173-179
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• 1996

The gene encoding gIII of bovine herpesvirus type 1 (BHV-1) PQ strain was cloned and expressed in baculovirus. Although the gIII gene is located in Hind III I fragment as the case of the other BHV-1 strains, differences in size and restriction endonuclease site within the fragment were identified. The gIII expression was predominantly detected on the surface on insect cells by indirect immunofluoresecnce assay using monoclonal antibody. The western blotting analysis also revealed the presence of expressed protein of a similar molecular size to the original gIII protein. The immunogenicity of expressed protein were tested in guinea pigs. The immunized guinea pigs with expressed protein developed the neutralizing antibodies against BHV-1.

• 한국정보처리학회:학술대회논문집
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• 한국정보처리학회 2012년도 추계학술발표대회
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• pp.741-742
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• 2012

This paper gives an asynchronously adaptive message passing scheme for selective retransmission of dropped packets based on a different fragment in size according to both the type of cause that makes packet loss and variation of traffic load in vehicle wireless communication networks. With the proposed scheme, it is possible to enhance the correctness for under-standing awareness of current network situation and to reduce the number of control packets by increasing or decreasing the size of a fragment along with the changes in network traffic load.

• 한국세라믹학회지
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• 제41권3호
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• pp.210-215
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• 2004

하수슬러지 소각재, 폐도자기 파편, 폐유약 및 저급점토를 이용하여 1,00$0^{\circ}C$에서 2시간 소성하여 투수블록용 다공질 세라믹스를 제조하여 제조인자에 따른 물리 $.$역학적 특성, 투수특성 및 동결음해특성을 조사하였다. 폐도자기 파편의 크기가 동일한 시편은 소성온도와 소각재의 첨가량이 증가함에 따라 부피비중과 압축강도는 증가하였으나, 기공률과 투수성은 감소하였다. 소성온도와 소각재의 첨가량이 동일한 시편은 폐도자기 파편의 크기가 증가하면 부피비중과 압축강도는 감소하고 기공률과 투수성은 증가하였다. 폐도자기 파편의 크기가 1∼2 mm이고 1,00$0^{\circ}C$에서 2시간 소성한 30A60F시편은 부피비중 2.17, 기공률 46.2%, 압축강도 221 kgf/$\textrm{cm}^2$ 및 통과전하량이 3,150 coulombs이었으며, 동결융해 저항성이 우수하여 투수블록으로 사용이 가능하였다. 투수블록 시편에 함유된 중금속은 허용기준치보다 낮아 무해하였다.

• 한국미생물·생명공학회지
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• 제13권3호
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• pp.297-302
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• 1985

미생물중 urease생성능이 아주 강한 B. pasteurii의 Hind III partial digest 된 chromosomal DNA를 E. coli-B. subtilis bifunctional plasmid vector pGR 71으로 E. coli RR1 균주에 cloning 하므로써 그 urease gene을 expression시킬 수 있었다. 그러나 B. subtilis에서는 insertion DNA fragment의 deletion으로 expression되지 않았다. Cloning된 E.coli RR1 균주로부터 분리 정제한 urease gene함유 Plasmid(pGU66)의 restriction map을 작성하여 본 결과 7.1 Mdal의 insertion fragment가 삽입된 12.6Mdal의 plasmid에 Hind III, Bgl II, Xba I, Sal I등 몇 개의 cleavage site 위치를 찾을 수 있었다. Cloning된 E. coli의 urease는 periplasmic space에 많은 비율로 축적되며, 그 효소학적 성질은 donor인 B.pasteurii의 그것과 매우 유사하였다.