• KSBB Journal
• /
• v.29 no.1
• /
• pp.29-35
• /
• 2014

In this study, antioxidant and antimicrobial activities of ethanolic crude extract and its five different solvent subfractions (namely, ethyl acetate fraction, n-butanol fraction, chloroform fraction, n-hexane fraction and the aqueous fraction) from a mixture of four different medicinal herbs (Galla rhois, Achyranthes japonica Nakai, Terminalia chebula Retz and Glycyrrhiza uralensis) were investigated. Among all the tested mixture combination of four medicinal herbs, 5:3:1:1 ratio of Galla:Achyranthes : Terminalia : Glycyrrhiza had the best antimicrobial effects against four strains of microorganisms (Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Escherichia coli) and exhibited the highest DPPH radical-scavenging activity. Further sub-fractions with solvents were screened for antioxidant and antimicrobial activities. Antioxidant activity in order was ethyl acetate fraction > n-butanol fraction > chloroform fraction > nhexane fraction > aqueous fraction. The n-butanol extracted fraction showed the highest level of antimicrobial activity in com- parison to other fractions. In addition, all those fractions did not show any cytotoxicity against human skin cell CCD-986sk. These results suggest that 5:3:1:1 combination extracts of medicinal herbs (Galla : Achyranthes : Terminalia : Glycyrrhiza) may be potentially used as a safe natural antimicrobial preservative.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.32 no.1
• /
• pp.119-123
• /
• 2003

From the 80% ethanol extraction of onion flesh, fraction I,II and III were separated by Sephadex LH-20 column chromatography. Fraction II and III had a high inhibitory effect on ACE and xanthine oxidase. The ACE was about 80% at 50 ppm of fraction IIand III. The xanthine oxidase was about 82.5% at 100 ppm of fraction In. This research was studied to investigate the effects of the fraction In isolated from onion on the reduction of fat accumulation in rats fed high fat diet for 2 months. The metabolism was examined by analyzing the fat in serum and liver. It was shown that cholesterol in serum was remarkably reduced in onion fraction In as compared with those of control.

• Microbiology and Biotechnology Letters
• /
• v.47 no.1
• /
• pp.54-63
• /
• 2019

Rubus coreanus Miquel (RCM), also known as Korean blackberry or bokbunja, is used as a South Korean traditional medicine to treat acne and inflammatory skin conditions. The antimicrobial activity of RCM root and its active compounds remain unclear. In this study, we prepared a 50% ethanol fraction, ethyl acetate fraction, and acid-treated ethyl acetate fraction (aglycone fraction) of RCM root, and evaluated antibacterial activities against the skin pathogens Staphylococcus aureus, Pseudomonas acnes, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. In a paper disc assay, all fractions of RCM root showed antimicrobial activities against the five skin pathogens. The ethyl acetate fraction displayed 6-, 12-, and 2-fold higher minimal inhibitory concentration (MIC) than the 50% ethanol fraction against S. aureus, E. coli, and P. acnes, respectively. The aglycone fraction displayed 2-fold higher MIC than methyl paraben against P. acnes, S. aureus, E. coli, and P. aeruginosa. The ethyl acetate fraction displayed a minimal bactericidal concentration (MBC) similar to that of methyl paraben, and the aglycone fraction showed 2- to 4-fold higher MBCs than those of methyl paraben. In particular, the ethyl acetate fraction was not cytotoxic and showed thermal stability after incubation at high temperatures ($60-121^{\circ}C$). Finally, the ethyl acetate fraction was separated and four components were identified: procyanidin C, propelagonidin dimer, ellagic acid, and methyl ellagic acid acetyl pentose. The compounds showed high antibacterial activities. These results suggest that RCM root is potentially applicable as a natural preservative in cosmetics.

• Food Science and Preservation
• /
• v.8 no.2
• /
• pp.224-230
• /
• 2001

To develop a material for functional food, the fraction Ⅰ,II and Ⅲ of onion extract were separated by Sephadex LH-20 column. The revealed purification levels of each fraction were 5.2%-6.5%, 12.2%-14.5% and 82.6%-86.7%, respectively. A functional activity test was performed with fraction Ⅲ. The ACE inhibition activity was about 50% at 5mg and about 80% at 10mg. The XOase inhibition activity of Korean Changnyong Daego was 84%, which was the highest. At the experiment of tyrosinase inhibition was detected 21-36% at 10mg. The cholesterol dose in mice blood was as low as low as 79$\pm$5(mg/100 mL) after the intake of fraction Ⅲ. Cholestrol dose in liver was decreased to below 50%. The results show that Sephadex LH-20 column method was very useful as a fraction method for the development of functional food material nsing onions.

• YAKHAK HOEJI
• /
• v.30 no.4
• /
• pp.174-179
• /
• 1986

The thymus weight of the mouse was 54.1% in protein malnutritive diet group and 39.2% in group treated with saponin fraction of Panax ginseng in comparison to normal diet group. This decreasing effects of protein malnutritive diet and saponin fraction on the thymus weight practically disappeared after four weeks. The saponin fraction showed no effect on the spleen weight of the mouse. The supplement of the saponin fraction enhanced total peritoneal exudate cells, content of total serum protein and albumin content of the mouse, each 45, 8 and 10% respectively in comparision to that of normal diet group. And these values in protein malnutritive diet group were 61.2, 83.6 and 87.0% respectively in comparision to that of normal diet group, and recovered to the level of normal diet group by the supplement of the saponin fraction. The electrophoregram of the serum protein of the mouse fed with protein malnutritive diet was different from that of the mouse fed with normal diet, but this difference practically disappeared by the supplement of the saponin fraction.

• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.2
• /
• pp.188-191
• /
• 2000

Appetite stimulant ether-soluble neutral extract of a high-grain compound feed was separated into four fractions with an open column of silica gel chromatograph using four carrier solutions containing n-pentane and diethyl ether as 100:0 (Fraction A), 90:10 (Fraction B), 75:25 (Fraction C) and 0:100 (Fraction D). The stimulative effects of the fractions were examined by comparing the intake of hay (with or without the fractions) in two-choice test bioassays with cattle. The Fractions A, B and C of the neutral extract stimulated (p<0.05) hay consumption in cattle, whereas Fraction D had no effect on selective feeding when compared with the control fraction. Furthermore, Fractions A, B and C had higher (p<0.05) feeding stimulative indices (FSI) than that of the Fraction D. The results suggest that chemical stimulants to increase palatability of hay are present in the neutral Fractions A, B and C of high-grain concentrate.

• Proceedings of the KSME Conference
• /
• 2000.04b
• /
• pp.721-727
• /
• 2000

Impedance method was carried out to design the electrode that can measure the void fraction of the bubbly flow in pool reservoir. To find out the optimum electrode shape, Styrofoam-tests were performed in a specially designed acrylic reservoir. Three kinds of electrodes were designed to compare the characteristics of water-air flow. The resistance was increased as the void fraction increased and the capacitance was decreased as the void fraction increased. The resistance is a main parameter to express the nature of the water-air flow in impedance method. Almost all the values of impedance were involved in resistance. The degree of deviation from the mean-resistance values showed reasonable results. Electrode type-I expressed excellent results among the three electrode shapes. The impedance values in void fraction 0-10% were similar to those of Maxwell's equation. But the impedance values in void fraction 10-20% were not similar to those of Maxwell' equation because of the edge effect near electrode.

• The Korean Journal of Pharmacology
• /
• v.5 no.2
• /
• pp.93-103
• /
• 1969

Saponin, essential oil, fat oil and alkaloidal fraction were fractionated from the ethanol extract of fanax ginseng. Effect of each fraction of Panax ginseng upon the temperature response induced by reserpine, chlorpromazine and nembutal was investigated in mice, so as to secure some hidden facets of each fraction of Panax ginseng acting upon central nervous system. The authors could arrive at some results, that is: (1) Inhibitory effect of Panax ginseng upon temperature decline induced by nembutal and chlorpromazine and potentiating action upon hyperthermia induced by reserpine reside mainly in saponin fraction and slightly in essential oil fraction. (2) The effect of Ginseng saponin on temperature response in nice seems to be related with the liberation of serotonin and histamine.

• Proceedings of the KSRS Conference
• /
• 2007.10a
• /
• pp.468-470
• /
• 2007

This paper is to present an approach for generating coarse resolution (MODIS data) fraction images of forested region in Korea peninsula using forest type area fraction derived from high resolution data (ASTER data) in regional forest area. A 15-m spatial resolution multi-spectral ASTER image was acquired under clear sky conditions on September 22, 2003 over the forested area near Seoul, Korea and was used to select each end-member that represent a pure reflectance of component of forest such as different forest, bare soil and water. The area fraction of selected each end-member and a 500-m spatial resolution MODIS reflectance product covering study area was applied to a linear mixture inversion model for calculating the fraction image of forest component across the South Korea. We found that the area fraction values of each end-member observed from high resolution image data could be used to separate forest cover in low resolution image data.

• Research in Mathematical Education
• /
• v.16 no.4
• /
• pp.217-232
• /
• 2012

While many studies on early algebra have been conducted, there have been only a few studies on the operation sense as the fundamental element of algebraic thinking, especially the fraction operation sense. This study explored the dimensions of fraction operation sense and then investigated students' fraction operation sense. A total of 183 of sixth graders were surveyed and 5 students who showed high operation sense were clinically interviewed in order to analyze their algebraic thinking in detail. The results showed that students had a tendency to use direct calculation or employ inappropriate operation sense rather than to use the structure of operation or the relation between operations on the basis of algebraic thinking. This study implies that explicit instruction on early algebra is necessary from the elementary school years.