• The Korean Journal of Mycology
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• v.23 no.2 s.73
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• pp.121-128
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• 1995

The characteristics of protein-bound polysaccharides (PBP) which were isolated and purified from the sawdust mycelia of Agrocybe cylindracea were investigated. The yield of crude protein-bound polysaccharides (Fr.CB) extracted with boiling water and precipitated with 95% ethanol, was 0.74% based on the original sawdust mycelia. The Fr.CB was purified by the membrane filtration, ion exchange chromatography and gel filtration. The Fr.B fraction of which the molecular weight is over 300 KDa, was isolated from the Fr.CB using membrane filtration, and the yield was 38.6% based on the Fr.CB. This result indicates that high molecular protein-bound polysaccharides are the dominent components of the Fr.CB. Two fractions (Fr.B-1, Fr.B-2) were also isolated from the Fr.B using ion exchange chromatography, and the yields were 17.3% (Fr.B-1) and 10.3% (Fr. B-2), respectively. The Fr.B-1 was concentrated and gel-filtrated, and the single peak, thought to be nearly pure protein-bound polysaccharides, was obtained. The yield of final fraction $(Fr.B-1-{\beta})$ was 42.5% based on the Fr.B-1. The molecular weight of $Fr.B-1-{\beta}$ was nearly 710 KDa. The monosaccharides' composition of $Fr.B-1-{\beta}$ was analized by HPLC, and glucose was the dominent component, and fucose and galactose were also detected. The result of amino acid analysis was that glutamic acid and analysis were detected to a significant level, and cysteine was not detected.

• Journal of the korean academy of Pediatric Dentistry
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• v.39 no.3
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• pp.293-300
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• 2012

In case of treating the functional type of anterior cross bite, removing the cause in early stage prevents from turning to skeletal type, leads to perform normal function, and has improved facial appearance. Functional Regulator III (FR III), one kind of the Functional regulator(FR)s suggested by R$\ddot{o}$lf Fr$\ddot{a}$nkel in 1966, applied to patients with the functional and skeletal anterior crossbite in early mixed, and permanent dentition. This appliance improves unbalanced power condition by blocking abnormal muscle-power effect, so that normal growth can be expected. In this case report, favorable results were obtained by selecting clinical cases of children in their early mixed dentition with functional cross bite. 1. FR IIIs were applied to patients with anterior crossbite for 5~6 months. Anterior crossbite patients were corrected favorably, nevertheless they didn't show any horizontal skeletal-changes by buccal shields. 2. Normal occlusion and esthetic facial contour were achieved from dental movement of maxillary and mandibular anterior teeth while the mandible rotates to posterior and inferior direction.

• The Korean Journal of Mycology
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• v.32 no.1
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• pp.23-30
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• 2004

Neutral salt-soluble, hot water-soluble and methanol-soluble materials (hereinafter referred to Fr. NaCl, Fr. HW and Fr. MeOH, respectively) were extracted from sclerotium of Grifola umbellata. The Fr. NaCl and Fr. HW did not show any direct cytotoxicity against NIH3T3, Sarcoma 180 and MCF-7, but Fr. MeOH showed cytotoxicity against these cell lines at the concentration of $1,000\;{\mu}g/ml$. Intraperitoneal injection with Fr. NaCl showed antitumor effect with life prolongation of 66.7% and decrease the number of Sarcoma 180 cells of 54.2% in mice inoculated with Sarcoma 180. Fr. NaCl improved the immunopotentiation activity through alternative complement pathway and the alkaline phosphatase activity by $85.05{\sim}88.73%$ and 6 folds, respectively. The number of peritoneal exudate cells and the circulating leucocytes were increased by 1.7 and 3.6 folds in the Fr. NaCl treating group compared with the control group, respectively. The weight of immunoorgans such as liver, spleen and thymus were also gradually increased. The hematological analysis of the Fr. NaCl group was similar with that of the control group. The total polysaccharide and protein contents of Fr. NaCl were 98.25% and 1.44%, respectively. These results indicate that the antitumor activity of Fr. NaCl was exerted through immunopotentiation, but not through cytotoxicity against the tumor cells.

• The Korean Journal of Applied Statistics
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• v.8 no.2
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• pp.87-97
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• 1995

When there is no main effcts or only one main effect in a $2 \times 2$ factorial design, Type I error rates and power for the rank transformed F test (FR test) for interaction are nearly equal to those of the classical F test. However the power of FR test is quite superior under the exponential distribution rather than the of FR test is quite superior under the exponential distribution rather than the normal distribution. Meanwhile when both main effects are in the model, Type I error rates of FR test, compared with those of F test, decrease as the effect size increases and are dependent on the fashion in which main effects are constructed. In addition, the power of FR test increases as the effect size and the sample size increase and is highly dependent on the manner in which main effects are constructed and the type of population distribution.

• The Korean Journal of Mycology
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• v.9 no.2
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• pp.77-91
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• 1981

This study has been carried out for the publication of colored illustrations of Korean fungi. The fresh higher fungi were collected and photographed, for the most part, at Gwangneung, Kyonggi Province, Mt. Yongmun in Kyonggi Province, Mt. Sokri in Chungcheong Province, Mt. Jiyee in Kyeongsang Province, Mt. Mudeug in Jeonra Province, and Mt. Hanra in Jeuju Island from September 1, 1980 to August 31, 1981. These higher fungi were made colored slides and dried specimens, then classified. According to the investigated result, colored slides and classification for the common 151 species were completed among 620 spp. which were known all of the fungi in Korea. They were included 2 classes, 37 families, 89 genera, 149 species, 1 variety and 1 forma. Their world distributions were investigated. Fifteen species of them, that is, Aleuria aurantia (Fr.) Fuckel, Patella scutellata (St. Amans) Lambotte, Calocora viscosa Fr., Hygrocybe turunda (Fr.) Karst, Suillus pictus (Peck) Smith et Thiers, Marasmius purpureostriatus Hongo, Pleurocybella porrigens (Fr.) Sing., Lepiota bresadoiae Schulzer, Russula aurata Fr., Lactarius hygrophoroides Berk. et Br., Thelephora multipartita Fr., Inonotus mikadoi (Lloyd) Imaz, Antrodia mollis (Sommerf.) Karst, Ganoderma neo-japonicum Imaz. and Tyromyces lacteus (Fr.) Murr. were found to be new in Korea. In addition their common names and descriptons were prepared. According to study plan, this paper pressed only one page in colored plate among 151 species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.5
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• pp.519-523
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• 2002

Soluble polysaccharide (SP) from green layer, Enteromorpba prolifera was extracted 3 times with distilled water at 100$^{\circ}C$ for 2 hrs and fractionated with cetylpyridinium chloride (CPC) and ion exchange chromatography (DEAE Separose CL-6B). The SP amounted to $23.7\%$ of the dry seaweed weight and contained $68.8\%$ carbohydrate. It was mainly constituted of rhamnose, glucose, xylose, sulfate and uronic acid and was fractionated with CPC into three (CPC-S, CPC-PS, CPC-PP) tractions. The major acid fraction CPC-PS accounted for $10.2\%$ of the dry algal weight. CPC-PS was further fractionated on DEAE Sepharose CL-6B into Fr-1 ($8.0\%$), Fr-2 ($35.8\%$), Fr-3 ($23.7\%$) fractions. The Fr-3 fraction contained $2.2\%$ protein, $21.4\%$ sulfate, $15.3\%$ uronic acid, and $72.4\%$ polysacchnrides composed of rhamnose, xylose and glucose. The Fr-2 fraction, which was richer in uronic acid ($17.5\%$) and poorer in sulfate ($19.0\%$) and total sugar ($68.8\%$) than the Fr-3, had a sugar composition close to that of Fr-3. The average molecular weights of Fr-2 and Fr-3 were 510,000 and 830,000 daltons, respectively. Fr-3 turned out to be homogeneous by cellulose acetate electrophoresis.

• Journal of the Korea institute for structural maintenance and inspection
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• v.13 no.6 s.58
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• pp.88-96
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• 2009

In this Study, FR-ECC(Fire Resistance Engineered Cementitious Composites) in which at same time it can improve the endurance and fire-resistance efficiency of a Structure was developed, and the experimental study such as thermal characteristic, Fire-resistance efficiency, and etc was performed for using FR-ECC as the repair materials for building and civil Structure. Moreover, it was evaluated about the field applicability of FR-ECC. As a result, FR-ECC is superior to the existence fire resistance repair mortar in strength and durability property. Also, FR-ECC was exposed to have the characteristic of being excellent than existence fire resistance mortar in the field applicability.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.24 no.7B
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• pp.1261-1267
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• 1999

Frame relay (FR) service via an FR interface over a common asynchronous transfer mode (ATM) backbone network requires an FR-to-ATM interworking unit (IWU), the functionality of which directly impacts the engineering of the overall network in terms of capacity required, quality of service (QoS), and the interference among different types of services. This paper presents performance analysis of frame relay traffic in IWU. Additionally frame relay traffic policing using leaky bucket algorithm in this traffic model is carried out through computer simulation. Three measurements, the percentage of frames accepted as committed, the percentage of frames that accepted but marked eligible for diacard, and the percentage of frames that accepted that are discarded because of insufficient number of credits, are simulated variously in this paper.

• The Transactions of the Korea Information Processing Society
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• v.4 no.10
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• pp.2421-2433
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• 1997

In this paper, based on the relative logical storage cost of each movie by the difference of service request probability, we propose and implement the SDU(Separate Data Use) method and the EDU(Existing Data Use) method to provide FF(Fast Forward) and FR(Fast Reverse) function in efficiently through analysing MPEG bit stream and frame characteristics. By selecting and applying the FF/FR function providing method based on the service request probability of movie, the proposed method can reduce the cost of FF/FR functions for total serviced movies.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1993.04a
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• pp.141-141
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• 1993

말징버섯 Calvatia craniformis의 culture broth 40 liter를 여과하여 얻은 균사채를 열수 추출하여 진한 갈색 건조분말(Fr. A) 13.1g을 분리하였다. Fr. A에 대하여 DEAE-cellulose ion chromatography를 시행하여 중성분획인 흰색 분말 (Fr. B) 2.50g을, 산성분획인 갈색 분말 (Fr. C) 3.50g을 각각 분리하였다. Fr. B 500mg에 대하여 Sepharose CL-4B gel filtration chromatography를 시행하여 흰색분말 Fr. D(Calvatan) 350mg을 분리하였다. Calvatan 350mg에 대하여 Con A-Sepharose 4B affinity chromatography를 적용하여 미흡착 분획인 Fr. F($\alpha$-form)와 흡착 분획인 Fr. E ($\beta$-form)로 정제하였다. 항암작용의 기전을 구명하고자 마우스에 대하여 면역학적 실험을 시행하였다. macrophage의 활성에 대한 영향을 조사하였던 바, 투여군의 활성화된 macrophage에 의해 유리되는 superoxide anion의 양은 대조군에 비해 1.4배 증가하였고 Calvatan 투여군의 용혈반 형성세포(PFC)는 대조군에 비해 3.1배 증가하였다. 화학 분석에 의해, Calvatan은 다당체 87.2%, 단백질 1.8% 및 hexosamine 1.3%로 구성되어 있었다. 따라서 항암성 분획들은 protein-bound polysaccharide임을 알 수 있었다. 또한 각 분획의 다당체를 구성하고 있는 단당류는 glucose, galactose, mannose 및 xylose 였으며 단백질 부분은 16종의 아미노산으로 구성되어 있었다. IR 스펙트럼은 3300-3400 $cm^{－1}$에서 0-H stretching frequency, 2900 $cm^{－1}$ 에서 C-H stretching frequency, 1630 $cm^{－1}$ 에서 C-0 stretching frequency. 1000-1100 $cm^{－1}$에서 C-H 및 C-0 bending frequency를 나타내는 다당체의 전형적인 특성을 보여주었다.을 보여주었다.