• Biotechnology and Bioprocess Engineering:BBE
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• 제9권6호
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• pp.523-527
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• 2004

Recombinant Saccharomyces cerevisiae expression systems were developed to pro­duce a novel human anti-angiogenic protein called LK8, an 86 amino-acid kringle fragment pro­tein with three disulfide linkages. Galactose-inducible LK8 expression plasmid was constructed, and LK8 production levels by four S. cerevisiae strains were compared in order to select an op­timal host strain. S. cerevisiae 2805 was the most efficient among the strains tested. Elevating the LK8 gene copy number through multiple integration using 8-sequences as target sites re­sulted in more than a two-fold increase in the LK8 production level compared with the plasmid­based expression system. The maximum LK8 protein concentration of 25 mg/L was obtained from batch cultivation of the yeast transformant that harbors 16 copies of the LK8 gene. In con­clusion, the strain integrated with the multiple LK8 gene secreted the protein with relatively high yield, although, the increased LK8 gene dosage over 11 copies did not lead to further en­hancement in batch cultivations.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.545-549
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• 2003

After irradiation of a cloned dextransucrase gene (dsrB742) with ultrasoft X-ray, an E. coli transformant (pDSRB742CK) was first developed for the expression of an extracellular dextransucrase, having increased activity and the synthesis of a highly branched dextran. Seven nucleotides of the parent gene (dsrB742) were changed in the nucleotide sequences of dsrB742ck. Among them, four nucleotides were changed at the ORF of dsrB742, resulting in a 30 amino acids deletion in the N-terminal of DSRB742 dextransucrase. The activity of DSRB742CK dextransucrase in culture supernatant was approximately 2.6 times higher (0.035 IU/ml) than that of the DSRB742 clone. The pDSRB742CK clone produced DSRB742CK dextransucrase when grown both on a sucrose medium (inducibly) and on a glucose medium (constitutively). The DSRB742 clone did not produce dextran constitutively on a glucose medium. DSRB742CK dextran had 15.6% branching and 2.7-times higher resistance to dextranase hydrolysis compared to DSRB742 dextran. $^{13}C-NMR$ showed that DSRB742CK dextran contained ${\alpha}-(1{\rightarrow}3)$ branch linkages that were not present in DSRB742 dextran.

• 한국문헌정보학회지
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• 제27권
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• pp.229-263
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• 1994

The main objective of this article lies in discussing dependecy issues as related to information development in NICs. The theoretical foundation was drawn from theories of 'dependency' and of 'information societies,' The main assertion of the dependency school (the more dependency, the less development) was applied for a test of the relationship between dependency on foreign scientific and technical information (STI) resources and indigenous STI development in NICs. Utilizing a case study method, this study explored the causes and results of dependency on foreign STI in a leading NIC­the Republic of Korea (ROK). First identified were the economic factors that might have influenced the ROK's dependency on foreign STI. Also investigated were the social factors that might have had associations with the R&D personnel's dependency on foreign STI. Regarding the development of information area, this study discussed general features in the process of producing, organizng, and gatekeeping indigenous STIIdentified regarding the ROK's information dependency was the R&D personnel's pattern of using foreign STI resources as well as the country's dependency on foreign STI resources. This study also discussed the socio-economic progress toward an information society in ROK for the past decades. Finally, specific linkages between the selected five variables were examined on the basis of the proposed four hypotheses.

• 응용통계연구
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• 제17권2호
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• pp.239-252
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• 2004

국내외 시장간에 정보의 이동이 신속해지고 유사 시장간에 상호 연관성이 심화되면서 한미간 주가동조화현상은 강화된 것으로 알려져 있다. 본 연구에서는 한미 증시간에 어떠한 역학관계가 존재하는가를 총체적으로 결정해 보았다. 분석 결과 주가가 전반적으로 비슷한 동향을 보이는 시기에는 한미 증시간의 인과관계가 상대적으로 복잡한 반면, 한미 간의 주가가 상이 한 동향을 보이는 시기에는 인과관계가 단순한 것으로 나타났다. 특히 나스닥지수로 부터 국내 주가지수로의 인과관계가 뚜렷이 존재하는 것으로 판명되어 IT산업 불황기에 침체에 빠진 국내 증시가 첨단산업이 주축을 이룬 나스닥시장의 동향에 민감한 현실이 그대로 입증되고 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제13권8호
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• pp.1162-1172
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• 2000

The research was conducted to quantify farm and household characteristics of sheep farmers, evaluate farmer access to and the effectiveness of livestock services in sheep farming areas, and examine regional constraints to improving sheep productivity and profitability in Nepal. A rapid diagnostic socioeconomic survey of 200 sheep farmers was carried out in 1996 and all four ecological regions (Trans-Himalayan, Mountains, Hills and Terai), each with a distinct local sheep breed, were represented in the survey. Six major constraints were identified: (a) poor performance of local sheep breeds, (b) a serious seasonal deficit of pasture and other feed, (c) the lack of an organized market for wool and meat, (d) poor access to agricultural credit, (e) primitive shearing equipment, and (f) an inadequate supply of drinking water for sheep. Strategies to assist farmers develop their sheep management skills, improve access and quality of support services, improved technology adaptable to local conditions and effective linkages with local carpet and meat industry are likely to overcome the constraints and alleviate persistent poverty faced by sheep farmers in Nepal.

• 로봇학회논문지
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• 제11권4호
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• pp.193-204
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• 2016

This paper presents an anthropomorphic finger prosthesis for amputees whose proximal phalanx is mutilated. The finger prosthesis to be proposed is able to make the amputees to perform the natural motion such as flexion/extension as well as self-adaptive grasping motion as if normal human finger does. The mechanism of finger prosthesis with three degrees-of-freedom (DOFs) consists of two five-bar and one four-bar linkages. Two passive components composed of torsional spring and mechanical stopper and only one active joint are employed in order to realize an underactuation. Each passive component is installed into the five-bar linkage. In order to activate the finger prosthesis, it is required for the user to flex and extend the remaining proximal phalanx on the metacarpophalangeal (MCP) joint, not an electric motor. Thus the finger prosthesis conducts not only the natural motion according to his/her intention but also the grasping motion through the deformation of springs by the object for human finger-like behavior. In order to reveal the operation principle of the proposed mechanism, kinematic analysis is performed for the linkage design. Finally both simulations and experiments are conducted in order to reveal the design feasibility of the proposed finger mechanism.

• Journal of the Korean Wood Science and Technology
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• 제36권2호
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• pp.63-72
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• 2008

As a part of abating formaldehyde emission of urea-formaldehyde (UF) resin adhesive, this study was conducted to investigate chemical structures of UF resin adhesives with different formaldehyde/urea (F/U) mole ratios, using carbon-13 nuclear magnetic resonance ($^{13}C$-NMR) spectroscopy. UF resin adhesives were synthesized at four different F/U mole ratios such as 1.6, 1.4, 1.2, and 1.0 for the analysis. The analysis $^{13}C$-NMR spectroscopy showed that UF resin adhesives with higher F/U mole ratios (i.e., 1.6 and 1.4) had two distinctive peaks, indicating the presence of dimethylene ether linkages and methylene glycols, a dissolved form of free formaldehyde. But, these peaks were not detected at the UF resins with lower F/U mole ratios (i.e., 1.2 and 1.0). These chemical structures present at the UF resins with higher F/U mole ratios indicated that UF resin adhesive with higher F/U mole ratio had a greater contribution to the formaldehyde emission than that of lower F/U mole ratio. Uronic species were detected for all UF resins regardless of F/U mole ratios.

• The Journal of Asian Finance, Economics and Business
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• 제8권2호
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• pp.77-88
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• 2021

The stock markets in the BRICS (Brazil, Russia, India, China and South Africa) countries are the leading emerging markets globally. Therefore, it is pertinent to ascertain the critical drivers of stock market development in these economies. The currrent study empirically investigates to identify the linkages between stock market development, key macro-economic factors and institutional factors in the BRICS economies. The study covers the time period from 2000 to 2017. The dependent variable is the country's stock market development and the independent variables consist of six macroeconomic variables and five institutional variables. The study employs a panel cointegration test, Fully Modified OLS (FMOLS), a Pooled Mean Group (PMG) approach and a heterogeneous panel non-causality test.The findings of the study indicate co-integration among the selected variables across the BRICS stock markets. Long-run estimations reveal that five macroeconomic variables and four variables related to institutional quality are positive and statistically significant. Further, short-run causalities between stock market capitalization and selected variables are detected through the test of non-causality in a heterogeneous panel setting. The findings suggest that policymakers in the BRICS countries should enhance robust macroeconomic conditions to support their financial markets and should strengthen the institutional quality drivers to stimulate the pace of stock market development in their countries.

• The Journal of Asian Finance, Economics and Business
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• 제9권3호
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• pp.83-94
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• 2022

The aim of this research is to examine the structural linkages between social responsibility, social capital, competitive advantages, and agricultural enterprise performance in China. This research focused on the role of social capital and competitive advantages in mediating the relationship between CSR and corporate performance. 492 employees from agricultural firms in Xinjiang, China, took part in the study. Confirmatory factor analysis and exploratory factor analysis were used to assess the measurement scales' reliability and validity. The associations between these four variables were investigated using structural equation modeling, and the mediating impact was tested using the Bootstrap method. Corporate social responsibility, social capital, and competitive advantage are all positively related to business performance, according to the findings. According to the results of the mediating effect test social capital and competitive advantage partially mediated the relationship between corporate social responsibility and business success. Unlike earlier research, this study focused on the impact of social responsibility on agricultural enterprise performance in impoverished rural areas. The findings of this study, in particular, benefit agricultural company management by revealing the role of social capital and competitive advantage in mediating the relationship between corporate social responsibility and business performance.

• 한국전자통신학회논문지
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• 제3권4호
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• pp.304-312
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• 2008

본 연구에서는 KIX의 성과분석을 위해, 민간기업과 정부기관 구분하여 분석하였다. 민간기업의 적용되는 BSC 성과분석 4개 관점간의 논리적 연계성(Logical Linkage)이 모든 기업에는 동일하게 적용가능하나, 정부기관에서는 4개의 관점간 논리적 연계성 확보가 곤란하다. 이러한 논리적 연계성 상실은 정부기관의 KIX 사업에 BSC 적용의 제약요인으로 작용한다. 이러한 내부와 외부 고찰 관점과 연계하여 KIX의 BSC 관점은 품질(운영)관점, 이용자(고객) 만족도 관점, 경제적 비용효과(재무)관점으로만 구분해 KIX의 BSC 성과분석의 내외부적 관점에 관한 연계모형과 BSC의 내외부적 관점을 근간으로 KIX의 성과분석 모형을 만들어 KIX 전체 사업에 대한 성과결과를 분석하였다.