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Functional Analysis and Selection of Second-site Revertant of Escherichia coli 16S rRNA of C770G (Escherichia coli 16S rRNA 상의 770 위치에 염기치환을 가진 변이체 리보솜의 단백질 합성 능력을 회복시키는 이차복귀돌연변이체의 발췌)

  • Ha, Hye-Jeong;Ryou, Sang-Mi;Lee, Kang-Seok;Jeon, Che-Ok
    • Microbiology and Biotechnology Letters
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    • v.39 no.1
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    • pp.93-96
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    • 2011
  • It has been shown that a nucleotide substitution at position 770 in Escherichia coli 16S rRNA, which is implicated in forming the evolutionary conserved B2c intersubunit bridge, has a detrimental effect on ribosome function. In order to isolate second-site revertants that complement ribosomes containing C770G, we performed a random mutagenesis of the 16S rRNA gene and selected clones that could produce more CAT protein translated by specialized ribosome. One of the clones contained two nucleotide substitutions at positions 569 and 904 (C569G and U904C) and these mutations partially complemented the loss of protein-synthesis ability caused by C770G. Further studies using the isolated revertant will provide information about which part of 16S rRNA is interacting with C770 and the consequence of the structure formed by these interactions in the process of protein synthesis.

A Design for Realtime Monitoring System and Data Analysis Verification TA to Improve the Manufacturing Process Using HW-SW Integrated Framework (HW-SW 통합 프레임워크를 활용한 제조공정 개선을 위한 실시간 모니터링 시스템과 데이터 분석검증 TA설계)

  • Kim, Jae Chun;Jin, Seon A;Park, Young Hee;Noh, Seong Yeo;Lee, Hyun Dong
    • KIPS Transactions on Software and Data Engineering
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    • v.4 no.9
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    • pp.357-370
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    • 2015
  • Massive data occurred in a manufacturing place is able to fulfill very important roll to improve the manufacturing process. Domestic manufacturing business has been making an multilateral effort to react the change of manufacturing circumstance, but it undergoes many difficulties due to technical weakness. Coatings is a type of paint. It protect products by forming a film layer on the products and assigns various properties to those. The research of coatings is one of the fields studied actively in the polymer industry. The importance of the coatings in various industries is more increased. However, the industry still performs a mixing process in dependence on operator's experiences. In this paper, we propose a design for realtime monitoring system and data analysis verification TA to improve the manufacturing process using HW-SW integrated framework. Analysis results from the proposed framework are able to improve the coatings formulation process by collecting more quantitative reference data for work and providing it to work place. In particular, the framework may reduce the deterioration and loss cost which are caused by absence of a standard data as a accurate formulation criteria. It also may suggest a counterplan regarding errors which can be occurred in the future by deriving a standard calibration equation from the analysis using R and Design of Experiments about an error data generated in the mixing step.

Performance of Underwater Communication in Low Salinity Layer at the Western Sea of Jeju (제주도 서부 해역의 저염수층을 고려한 수중통신 성능)

  • Bok, Tae-Hoon;Kim, Ju-Ho;Lee, Chong-Hyun;Bae, Jin-Ho;Paeng, Dong-Guk;Pang, Ig-Chan;Lee, Jong-Kil
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.48 no.1
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    • pp.16-24
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    • 2011
  • The sound speed of seawater can be calculated by the empirical formula as a function of temperature, salinity and pressure. It is little affected by salinity because the average salinity is 34 psu and varies within a few psu seasonally and spatially in the ocean. Recently, low-salinity water of 24 psu flows into the western sea area of Jeju Island due to the flood of the Yangtze River in China during summer, affecting sound speed profile. In this paper, it was analyzed how environmental changes affected to the underwater communication - the sound speed of low-salinity water was calculated, and the communication channel was estimated by the simulated acoustic rays while the transmitting and receiving depth and the range were varied with and without the low-salinity layer. And The BER (Bit error rate) was calculated by BPSK(Binary phase shift key) modulation and the effects of the low-salinity water on the BER was investigated. The sound speed profile was changed to have positive slope by the low-salinity layer at the sub-surface up to 20 m of depth, forming acoustic wave propagation channel at the sub-surface resulting in the decrease of most of the BER Consequently, this paper suggests that it is important to consider changes of the ocean environment for correctly analyzing the underwater communication and the detection capability.

The Crystal and Molecular Structure of p-Phenylenediamine Dihydroperchlorate (p-Phenylenediamine Dihydroperchlorate의 결정 및 분자구조)

  • Ahn Choong Tai
    • Journal of the Korean Chemical Society
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    • v.21 no.5
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    • pp.320-329
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    • 1977
  • p-Phenylenediamine dihydroperchlorate, $C_6H_4N_2H_4{\cdot}2HC1O_4$, crystallizes in space group $P\={1}$ with $a=4.79{\pm}0.02,\;b=9.03{\pm}0.02,\;c=7.12{\pm}0.03{\AA},\;{\alpha}=109.4{\pm}0.2,\;{\beta}=79.6{\pm}0.2,\;r=104.6{\pm}0.2^{\circ},\;Z=1$. The structure has been solved by the Patterson and Fourier methods. The refinement by block-diagonal least-squares cycles gives R = 0.13 for 387 observed reflexions collected on equi-inclination Weissenberg photographs with CuK${\alpha}$ radiation. There are two different types of five hydrogen bonds. The first type consists of one trifurcated N${\cdot}{\cdot}{\cdot}$O hydrogen bond and the second of two normal N${\cdot}{\cdot}{\cdot}$O hydrogen bonds, both of which exist between the amino group and the perchlorate, groups. A p-phenylenediamine group is approximately planar within an experimental error and bonded to twelve perchlorates: ten perchlorates forming hydrogen bonds and two being contacted with the van der Waals forces. A perchlorate group is surrounded by six p-phenylenediamines and four perchlorates; among the six p-phenylenediamines, five of them are hydrogen-bonded, and the rest contacted with the van der Waals force.ce anaysis of our samples and investigated the variarions in the values of parameters obtained through fitting the theoretical impedance to the experimental impedance. The characters of the dielectric constant and the impedance showed abnormal variations for the 0.2 at K-doped NSBN ceramics, which we were able to interpret in terms of the variations in the number A-site vacancies with the K doping ratio. From these results, A-site vacancies are thought to be space charges that influence the ferroelectric properties of NSBN ceramics.

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Molecular Theory of Plastic Deformation (I). Theory (소성변형의 분자론 (제1보). 이론)

  • Kim Chang Hong;Ree Taikyue
    • Journal of the Korean Chemical Society
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    • v.21 no.5
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    • pp.330-338
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    • 1977
  • In order to elucidate the plastic deformation of solids, the following assumptions were made: (1) the plastic deformation of solids is classified into two main types, the one which is caused by dislocation movement and the other caused by grain boundary movement, each movement being restricted on a different shear surface, (2) the dislocation movement is expressed by a mechanical model of a parallel connection of various kinds of Maxwell dislocation flow units whereas the grain boundary movement is also expressed by a parallel connection of various kinds of Maxwell grain boundary flow units; the parallel connection in each type of movements indicates that all the flow units on each shear surface flow with the same shear rate, (3) the latter model for grain boundary movement is connected in series to the former for dislocation movement, this means physically that the applied stress distributes homogeneously in the flow system while the total strain rate distributes heterogeneously on the two types of shear planes (dislocation or grain boundary shear plane), (4) the movement of dislocation flow units and grain boundary units becomes possible when the atoms or molecules near the obstacles, which hinder the movement of flow units, diffuse away from the obstacles.Using the above assumptions in conjunction with the theory of rate processes, generalized equations of shear stress and shear rate for plastic deformation were derived. In this paper, four cases important in practice were considered.ted N${\cdot}{\cdot}{\cdot}$O hydrogen bond and the second of two normal N${\cdot}{\cdot}{\cdot}$O hydrogen bonds, both of which exist between the amino group and the perchlorate, groups. A p-phenylenediamine group is approximately planar within an experimental error and bonded to twelve perchlorates: ten perchlorates forming hydrogen bonds and two being contacted with the van der Waals forces. A perchlorate group is surrounded by six p-phenylenediamines and four perchlorates; among the six p-phenylenediamines, five of them are hydrogen-bonded, and the rest contacted with the van der Waals force.

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System Design and Evaluation of Digital Retrodirective Array Antenna for High Speed Tracking Performance (고속 추적 특성을 위한 디지털 역지향성 배열 안테나 시스템 설계와 특성 평가)

  • Kim, So-Ra;Ryu, Heung-Gyun
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.38A no.8
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    • pp.623-628
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    • 2013
  • The retrodirective array antenna system is operated faster than existing techniques of beamforming due to its less complexity. Therefore, it is effective for beam tracking in the environment of fast vehicle. On the other hand, it also has difficulty in estimating AOA according to multipath environment or multiuser signals. To improve the certainty of estimating AOA), this article proposes hybrid digital retrodirective array antenna systme combined with MUSIC algorithm. In this paper, the digital retrodirective array antenna system is designed according to the number of antenna array by using only one digital PLL which finds angle of delayed phase. And we evaluate the performance of the digital retrodirective array antenna for the high speed tracking application. Performance is studied by simulink when the speed of mobile is 300km/h and the distance between transmitter and receiver is 100m and then we have to confirm the performance of the system in multi path environment. As a result, the mean of AOA (Angle Of Arrival) error is $4.2^{\circ}$ when SNR is 10dB and it is $1.3^{\circ}$ when SNR is 20dB. Consequently, the digital RDA shows very good performance for high speed tracking due to the simple calculation and realization.

The Origin and Age of the Orbicular Granite Gneiss in Wangjungri, Muju (무주 왕정리 일대 구상 화강편마암의 성인과 형성시기)

  • Oh, Chang Whan;Lee, Byung Choon;Yi, Keewook
    • The Journal of the Petrological Society of Korea
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    • v.22 no.2
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    • pp.117-135
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    • 2013
  • Orbicular granite gneisses occur as a xenolith within two-mica leucogranites, together with early Paleoproterozoic metasedimentary xenoliths, in Wangjeong-ri, Muju area. The whole-rock chemistries and SHRIMP zircon Pb/U ages of the leucogranites indicate that they are S-type granitoids formed in the continental tectonic setting at $1875{\pm}75$ Ma. The SHRIMP age of monazites from the orbicular granite gneiss gives $1867{\pm}4$ Ma as a metamorphic age which is similar to the intrusion age of the two-mica leucogranite within the error range. The similar ages between zircons and monazites represent that the orbicular granite gneisses formed by metamorphism during the intrusion of the two-mica leucogranite; the metasedimetary xenoliths which sank within the parent magma of leucogranites were metamorphosed into orbicular granite gneisses by thermal metamorphism ($650-740^{\circ}C$, 4-6.5 kbar) due to the heat supplied from surrounding magma. During the thermal metamorphism, the core of orbicular granite gneiss mainly consisting of cordierite formed, and in some orbicular granitic gneisses, the leucocratic melt formed by melting of quartz and plagioclase in the core, squeezed out from core and crystallized around the core forming outer rim. The hydrothermal fluid at the late stage of magma differentiation penetrated into the orbicular granite gneisses resulting pinitization of cordierite into chlorite and sericite. As Muju orbicula granite gneiss was formed from sedimentary rocks, it is more appropriate to be called Muju orbicula granitic gneiss.

Studies on Thermophilic Flat-sour Bacteria in Soymilk: Isolation, Indentification and Determination of Heat Resistance (두유내의 고온성 Flat-sour 변질균의 분리.동정 및 열저항성에 관한 연구)

  • Chung, Je-Bong;Lee, Kyun-Hee;Sohn, Heon-Soo;Kim, Suk-Min
    • Korean Journal of Food Science and Technology
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    • v.20 no.2
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    • pp.218-224
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    • 1988
  • For the optimization of thermal processing conditions in soymilk process, 4 strains of thermoresistant flat-sour bacteria were isolated from soymilk. The isolates were aerobic spore-forming rods, and grew at $-65^{\circ}C$. Based on the morphological and physiological properties, all of the isolated strains were identified as Bacillus stearothermophilus. The heat resistance of spores of 3 isolates and Bacillus stearothermophillus ATCC 12980 as a reference was determined in soymilk(pH 7.0) and pH 7.0 buffer solution. For each of the spores studied, linear regression equations with standard error were presented for the thermal destruction at 110, 115, 121, and $125^{\circ}C$. It was not obvious that the components of soy milk increased the heat resistance of spores. Between the strains studied, variability was noted in the D values at the different temperature, and no one strain was consistently the most heat resistant at all the given temperatures. The average D value for the 4 strains was 77.27, 20.20, 2.76 and 1.39 min at 110, 115, 121 and $125^{\circ}C$, respectively, and the average z value was $8.36^{\circ}C$.

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Study on the design optimization of injection-molded DVD-Tray parts using CAE Simulation (플라스틱 DVD-Tray의 박막 사출성형을 위한 최적화 설계 Simulation에 관한 연구)

  • Chung, Jae-Youp;Kim, Dong-Hak
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.9 no.6
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    • pp.1726-1732
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    • 2008
  • Injection molding is one of plastic forming technology which can easily mass-produce plastic parts with various and even complex shape. The technology has lots of difficulties in making a good part due to phase change of material, high applied pressure, and fast melt flow speed in the cavity. To overcome the problems, they had to make trial and error method until the CAE(Computer Aided Engineering) could be a tool for concurrent engineering. In this paper, we investigate the optimal design for a plastic DVD tray part by systematic approach of the commercial CAE program. In design, we should consider two objectives which are both dimensional stability and cost-down. The dimension of the part is crucial because the tray should carry a DVD correctly, but the part is too thin to injection-mold easily. In order to improve the moldability, the mold is designed in the form of stack mold which is a kind of 4 hot runner system. In first, we changed the stack-mold system with one hot-runner to cost down, and decided the optimal position of the gate. After that, we investigate the effect of both the layout of cooling channels and the cooling temperature on the shrinkage of the DVD tray. A optimal simulation approach, the gate design is 2Gate#3 and the layout is Case2 cooling line as the optimal temperature of $70^{\circ}C$. The Moldflow and PC+ABS are used for the CAE program and material respectively.

Use of Multimedia Technologies in the Training of Physical Culture and Sports Specialists

  • Shevchenko, Olha;Bahinska, Olha;Markova, Olena;Broiakovskyi, Oleksandr;Bielkova, Tetyana;Honcharenko, Ivan;Bida, Olena
    • International Journal of Computer Science & Network Security
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    • v.22 no.7
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    • pp.245-251
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    • 2022
  • Educational reform in Ukraine encourages the use of multimedia technologies in the training of specialists in Physical Education and Sports, which is one of the promising directions of education development. Therefore, the article specifies the content of the terms "innovation" and "technology". For modern society, the introduction of multimedia technologies in education is not so much theoretical as pragmatic, since under condition of globalization it concerns its historical development and prospects associated with the so-called "high technologies".Our goal is to improve the training of Physical Education and Sports specialists by means of multimedia technologies. All of innovative technologies can be divided into four groups, depending on the appropriate form of educational activity for their use. The development of multimedia technologies in the training of specialists in Physical Education and Sport at the present stage of education development should be carried out in accordance with the criteria of manufacturability, which are presented in the article: scientism, to rely on the theoretical provisions of pedagogical science and methods of teaching the discipline, socially recognized educational goals, prospects for modernization of Education; consistency, which provides for the interaction of parts and the whole in the organization of the study environment, as a result of which the physical development of the young generation is an integral entity; guarantee, that is, the error between the planned and obtained results should be minimal; manageability, that is, full management of the stages of work of the teacher and students, which make up the completed cycle of actions; mass participation, for the purpose of applying the technology does not depend on the physical training of students, the pedagogical skill of the teacher and the type of educational institutions. The article presents the theory and method of organizing sports events and circuses in the training of specialists in Physical Education and Sports by means of multimedia technologies. In order to increase the level of physical development of a person, physical fitness and the state of health of students, which has a clear trend to constant deterioration, it is necessary to instill love for sports, carry out high-quality training and organize sports events using multimedia technologies. In the process of sports activities, the participants' mental education is carried out. There are two types of communication here: direct and indirect, which are described in the article.In games and sports competitions, there are many opportunities for forming rules of collective behavior. The main issues of the organization of sports activities by means of multimedia technologies have been clarified. During sports competitions, the tasks presented in Physical Education and sports classes are improved, which ensure the improvement of physical and theoretical training of the individual. The pleasure of sports, bright, emotional spectacles, confirmed by multimedia technologies, arises from the participation of the viewer in them.