• 한국동물위생학회지
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• 제45권4호
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• pp.305-316
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• 2022

Bordetella (B.) bronchiseptica, Mycoplasma (M.) felis, and Chlamydia (C.) felis are considered as main bacterial pathogens of feline upper respiratory tract disease (URTD). In this study, a new triplex quantitative real-time polymerase chain reaction (tqPCR) assay was developed for the rapid and differential detection of these bacteria in a single reaction. The assay specifically amplified three bacterial genes with the detection limit of below 10 copies/reaction. The assay showed high repeatability and reproducibility, with coefficients of intra-assay and inter-assay variation of less than 1%. Based on the diagnostic results of the assay using 94 clinical samples obtained from cats with URTD signs, prevalence of B. bronchiseptica, M. felis, or C. felis was 10.6%, 36.2%, or 6.4%, respectively, indicating that the diagnostic sensitivity was comparable to those of previously reported monoplex qPCR assays. The dual infection rates for B. bronchiseptica and M. felis or M. felis and C. felis was 2.1% or 3.2%, respectively. These results indicated that M. felis has been widely spread, and its co-infection with B. bronchiseptica or M. felis has been frequently occurred in Korean cat population. The developed tqPCR assay will serve as a promising tool for etiological and epidemiological studies of these three bacterial pathogens and the prevalence data obtained in this study will contribute to expanding knowledge about the epidemiology of feline URTD in Korea.

• 한국동물위생학회지
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• 제46권2호
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• pp.123-135
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• 2023

Feline calicivirus (FCV) is considered the main viral pathogen of feline upper respiratory tract disease (URTD). The frequent mutations of field FCV strains result in the poor diagnostic sensitivity of previously developed molecular diagnostic assays. In this study, a more sensitive real-time reverse transcription-polymerase chain reaction (qRT-PCR) assay was developed for broad detection of currently circulating FCVs and comparatively evaluated the diagnostic performance with previously developed qRT-PCR assay using clinical samples collected from Korean cat populations. The developed qRT-PCR assay specifically amplified the FCV p30 gene with a detection limit of below 10 copies/reaction. The assay showed high repeatability and reproducibility, with coefficients of intra-assay and inter-assay variation of less than 2%. Based on the clinical evaluation using 94 clinical samples obtained from URTD-suspected cats, the detection rate of FCV by the developed qRT-PCR assay was 47.9%, which was higher than that of the previous qRT-PCR assay (43.6%). The prevalence of FCV determined by the new qRT-PCR assay in this study was much higher than those of previous Korean studies determined by conventional RT-PCR assays. Due to the high sensitivity, specificity, and accuracy, the new qRT-PCR assay developed in this study will serve as a promising tool for etiological and epidemiological studies of FCV circulating in Korea. Furthermore, the prevalence data obtained in this study will contribute to expanding knowledge about the epidemiology of FCV in Korea.

• 한국정보시스템학회지:정보시스템연구
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• 제18권3호
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• pp.231-249
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• 2009

Recently, plant construction industries are enjoying a favorable business climate centering around developing countries and oil producing countries rich in oil money. This paper proposes a methodology of implementing case-based reasoning(CBR) system for managing knowledge like lessons learned and various documents accumulated in performing power plant construction projects which are receiving a lot of order from foreign countries such as the Middle East, etc. Our methodology is consisted of 10 steps : user requirement gathering, information modeling, case modeling, case base design, similarity function design, user interface design, case base building, CBR module development, user interface implementation, integration test. Also, to illustrate the effectiveness of proposed methodology, the real CBR system is implemented for the plant business division of 'H' company which has international competitiveness in the field of plant construction industry. At present, the implemented CBR system is successfully utilizing as storing, sharing, and reusing knowledge which is accumulated in performing power plant construction projects in the target enterprise.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2006년도 추계학술대회 논문집
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• pp.625-626
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• 2006

• 한국건설관리학회:학술대회논문집
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• 한국건설관리학회 2006년도 정기학술발표대회 논문집
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• pp.659-662
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• 2006

플랜트 산업은 2004년도 기준 해외 건설 수주액의 70%를 차지하는 매우 중요한 산업이다. 플랜트 산업의 기술력은 일부 시설관련 상세설계와 시공부문의 경쟁력을 확보한 상태이다. 그러나 플랜트 산업 가운데 고부가가치 창출이 가능한 기획, 기본설계 기술이 미흡하여 기술력 향상이 필요하다. 따라서 고부가가치 창출이 가능한 기획, 기본설계 기술 경쟁력을 향상시키기 위하여 플랜트 설계를 위한 표준코드의 문제점 및 개선방향을 제시하고자 한다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2012년도 심포지엄 및 춘계학술발표회
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• pp.331-332
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• 2012

• 한국조경학회지
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• 제25권2호
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• pp.62-72
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• 1997

This study was conducted to find out the hydroseeding timing of several herbaceous plants for the slope revegetation works. Four native plants and five introduced grasses were used for this experiment and were seeded on the cut slopes by hands in April, May, June, September and October. To identify the best seeding timing, germination percentage, ground covering rate, number of tillers and plant heights were investigated. There were wide differences in germination and ground covering rates of native plants are lower than those of cool-season foreign grasses and they show different germination rates according to seeding timing. Among them, Arundinella hirta var. ciliata shows the most apparent germinatin pattern according to seeding timing. 2. Seeding timing : Native plants tend to germinate well in May and June and cool-season foreign grasses in May and September. But Native plants show extremely low germination rates in autumn, so it is necessary to adjust the seeding rates when seeding in autumn. When seeding in May, it will be possible to use native plants-seed-mixture without using introduced foreign grasses. In sum, the best seeding timing of cool-season foreign grasses are May and September, and warm-season foreign grass is May and June. The best seeding timing of native plants seem to be in May and June.

• 한국건설관리학회논문집
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• 제9권1호
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• pp.107-117
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• 2008

국내 건설산업은 해외 시장에서 경쟁력을 높이기 위해 각종 R&D투자를 늘리고 있는 실정으로 본 연구에서는 국내 건설산업이 해외 건설시장에서 경쟁력을 보유하고 있는 대표 건설상품에 대한 경쟁력을 종합적이고 다각적으로 평가하였다. 이를 위하여 기존 연구에서 실시한 기업의 경쟁력과 기술수준을 평가한 자료를 근거로 기술수준을 측정할 기술수준지표와 기술수준 측정방법론을 선정하였으며, 기존연구에서 부족하였던 기술수준 및 기술경쟁력 평가의 심층성에 대한 보완 방안으로 해외 경쟁력 보유 상품을 선별한 후, 관련 업무 전문가를 대상으로 기술수준을 평가하였다. 전문가 대상 요소는 '기술경쟁력', '경영 및 지원 경쟁력'을 대상으로 실시하였으며 각 요소의 평가 점수를 통하여 선진국과의 경쟁력 보유여부를 평가하였다. 이러한 평가결과를 토대로 국내 건설 산업이 시장측면이나 기술측면에서 해외시장에서 경쟁이 가능한 유망 상품을 도출하고 국제경쟁력을 증대할 수 있는 방안에 대하여 연구를 진행하였다.

• Bulletin of the Korean Chemical Society
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• 제31권3호
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• pp.611-614
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• 2010

Substituted imidazolidin-2-ones deduced as potential inhibitors of IleRS by docking simulations were synthesized from an aziridine-2-carboxaldehyde. Reductive amination of an aziridine-2-carboxaldehyde with dipeptides for the substituents at N1 and followed by aziridine-ring expansion with triphosgene afforded 4-chloromethylimidazolidin-2-ones whose chloride were further manipulated towards phenylurea, pyrimidin-2-yl-urea or benzenesulfonamide at C4.

• 에너지공학
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• 제25권1호
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• pp.43-47
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• 2016

한수원 중앙연구원은 2007년부터 정부과제의 하나로 전기출력이 1500MWe급인 GEN III+ 원전 APR+를 개발해 왔다. APR1400 보다 안전성이 진전된 노형을 개발하기 위해 국내외에서 건설되거나 설계중인 ALWR의 개선된 설계특성을 조사하였다. 국내외의 원전건설 사업에 적합한 APR+ 표준설계를 개발하기 위해 신개념설계특성과 후쿠시마 원전사고 경험을 설계에 반영하였다. APR+의 안전성향상 표준설계 단계(2013.1 ~ 2015.12)에서 한 번의 경제성평가를 수행하였다. 설계 안전성향상 기술개발 단계에의 경제성 평가 결과 APR+ N-th호기는 국내석 탄화력 1000MWe급 대비 39.2% 경쟁력 우위인 것으로 평가되었다. 또한 APR+원전은 해외 원전 선진국 ALWR에 비해 동등 이상 수준의 경쟁력을 확보하는 것으로 평가되었다.