• Safety and Health at Work
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• 제13권1호
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• pp.78-85
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• 2022

Background: Veterinarians may encounter a variety of zoonotic pathogens in their work. Methods: We conducted two cross-sectional questionnaire studies among veterinarians in Finland. Participants were recruited during two Annual Veterinary Congresses. In 2009, 306 veterinarians participated in an extensive questionnaire study, and in 2016, 262 veterinarians participated in a more focused study that included two same questions. Results: In 2009, the majority (90.9%) of the participating veterinarians reported having been occupationally exposed to zoonotic pathogens. Zoonotic infections (15.0%), needle stick incidents (78.8%), bites (85.0%), as well as infected skin lesions (24.2%) were reported. In 2009, 8.2% of the participants fully agreed with the statement "I have good knowledge of zoonoses and their prevention"; in 2016, the proportion was 10.3%. The reported use of protective practices and personal protective equipment in connection with specific veterinary procedures indicated that there was room for improvement, particularly in protection from pathogens that are transmissible via inhalation and mucous membranes. Conclusion: The results confirm that veterinarians are commonly occupationally exposed to zoonotic pathogens. Education should aim to improve and maintain the knowledge of zoonoses and their prevention. Use of protective practices should be advocated.

• 한국식품위생안전성학회지
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• 제21권1호
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• pp.36-40
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• 2006

온천수에는 각종 무기물이 풍부하게 함유되어 있으며 특히 2가 이온들이 많이 들어있고 살균력을 갖춘 유황 성분이 있어, 식중독 및 병원성 미생물의 증식을 억제할 수 있는 가능성이 있다. 온천수의 식중독 및 병원성 미생물에 대한 억제 효과를 시험하기 위하여 식중독 미생물인 Bacillus cereus, Staphylococcus aureus 및 Escherichia coli, 피부병 원인균인 Candida albicans와 Trichophyton mentogrophytes, 위장병 원인균인 Helicobacter pylori을 대상으로 시험하였다. 본 실험에 사용한 온천수는 먹는물 수질기준에 따라 분석한 결과 불소함량이 기준치 1.5mg/L보다 높은 14.1 mg/L로 먹는물 수질기준에 적합하지 않으나 그 외 항목은 먹는물 수질기준에 적합하였다. 몇 가지 식중독 미생물이 온천수에서 생존하는 양상을 관찰한 결과 S. aureus 및 E. coli의 생존에 대해서는 별 영향을 미치지 않았으나, B. cereus의 균수는 감소시키는데 이는 온천수의 온도($41^{\circ}C$와 관계있는 것으로 판단되었다. C. albicans와 T. mentagrophytes등 피부병균과 위염 원인균(H. pylori)에 대해서도 온천수는 뚜렷한 증식 억제효과를 보이지 않으나 T. mentagrophytes는 온도에 영향이 있음을 확인하였다. 이상의 결과를 종합할 때 온천수는 식중독균이나 피부질환 혹은 위염 원인균에 뚜렷한 억제 효과는 나타나지 않으나 일부 고온($41^{\circ}C$)에 의한 균수의 감소 효과는 인정 되었다.

• 한국식품위생안전성학회지
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• 제34권6호
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• pp.509-518
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• 2019

사람이 섭취하는 식품 내의 항생제, 알레르기 유발 물질, 병원균 및 기타 오염물질의 수준을 모니터링하기 위해서는, 빠르고 정확하며 저렴한 비용으로 테스트 해야 한다. 이러한 문제 중 일부를 해결하기 위해 지난 10-15년 동안 진보된 기술(label-free biosensor assays)이 개발되어 왔다. 이 면역감지키트들은 실시간 측정이 가능하고, 높은 수준의 자동화를 제공하며, 향상된 처리율과 민감도를 가지고 있다. 또한, 기존의 방법과 비교하여 가격이 저렴하고, 덜 복잡하며, 분석 시간을 단축시켜주는 사용자 친화적 키트이다. 이 리뷰에서는 면역감지키트의 장단점, 그리고 미래의 식품안전검사에서의 사용성에 관한 것에 대해 논의해 볼 것이다.

• 한국환경과학회지
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• 제32권2호
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• pp.139-143
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• 2023

The effects of essential oils on pH, pathogens, and volatile fatty acids (VFA) in two poultry litters were investigated through a lab study. Essential oil-added poultry litters were randomly divided to two groups: control (200 g poultry litter) and Treatment (50 g thymol/Briefly, 200 g broiler litter was treated with or without 50 g thymol (Control and T1, respectively; 1 groups) and 200 g duck litter was treated with or without 50 g carvacrol (Control and T2, respectively; 2 group). Adding thymol to broiler litter increased the pH, reduced pathogens, and did not affect VFA. Interestingly, adding carvacrol slightly reduced the pH of duck litter, but had no significant effect on reducing pathogens and VFA. This difference is probably because the essential oil used and the properties of the two litters are different. In addition, pH was thought to control the odor generated from the litter, but this has not been proven. Further field studies should focus on clarifying this point.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2008년도 International Meeting of the Microbiological Society of Korea
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• pp.99-100
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• 2008

• 축산식품과학과 산업
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• 제8권1호
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• pp.21-27
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• 2019

• Parasites, Hosts and Diseases
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• 제56권5호
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• pp.521-525
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• 2018

Rodents are well-known reservoirs and vectors of many emerging and re-emerging infectious diseases, but little is known about their role in zoonotic disease transmission in Bhutan. In this study, a cross-sectional investigation of zoonotic disease pathogens in rodents was performed in Chukha district, Bhutan, where a high incidence of scrub typhus and cases of acute undifferentiated febrile illness had been reported in people during the preceding 4-6 months. Twelve rodents were trapped alive using wire-mesh traps. Following euthanasia, liver and kidney tissues were removed and tested using PCR for Orientia tsutsugamushi and other bacterial and rickettsial pathogens causing bartonellosis, borreliosis, human monocytic ehrlichiosis, human granulocytic anaplasmosis, leptospirosis, and rickettsiosis. A phylogenetic analysis was performed on all rodent species captured and pathogens detected. Four out of the 12 rodents (33.3%) tested positive by PCR for zoonotic pathogens. Anaplasma phagocytophilum, Bartonella grahamii, and B. queenslandensis were identified for the first time in Bhutan. Leptospira interrogans was also detected for the first time from rodents in Bhutan. The findings demonstrate the presence of these zoonotic pathogens in rodents in Bhutan, which may pose a risk of disease transmission to humans.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2006년도 제23차 정기총회 및 학술발표회
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• pp.7-16
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• 2006

Poultry products including meat and eggs constitute a major protein source in the American diet and disease - causing pathogens represent major challenges to the poultry industry. More than 95 % of pathogens enter the host through the mucosal surfaces of the respiratory, digestive and reproductive tracts and over the past few decades, the two main mechanisms used to control diseases have been the use of vaccines and antibiotics. However, in the poultry industry, there are mounting concerns over the ability of current vaccines to adequately protect against emerging hyper - virulent strains of pathogens and a lack of suitable, cost effective adjuvants. Thorough investigation of the immunogenetic responses involved in host-pathogen interactions will lead to the development of new and effective strategies for improving poultry health, food safety and the economic viability of the US poultry industry. In this paper, I describe the development of immunogenomic and proteomic tools to fundamentally determine and characterize the immunological mechanisms of the avian host to economically significant mucosal pathogens such as Eimeria. Recent completion of poultry genome sequencing and the development of several tissue-specific cDNA libraries in chickens are facilitating the rapid application of functional immunogenomics in the poultry disease research. Furthermore, research involving functional genomics, immunology and bioinformatics is providing novel insights into the processes of disease and immunity to microbial pathogens at mucosal surfaces. In this presentation, a new strategy of global gene expression using avian macrophage (AMM) to characterize the multiple pathways related to the variable immune responses of the host to Eimeria is described. This functional immunogenomics approach will increase current understanding of how mucosal immunity to infectious agents operates, and how it may be enhanced to enable the rational development of new and effective strategies against coccidiosis and other mucosal pathogens.

• 한국가금학회지
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• 제34권1호
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• pp.77-83
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• 2007

Poultry products including meat and eggs constitute a major protein source in the American diet and disease-causing pathogens represent major challenges to the poultry industry. More than 95% of pathogens enter the host through the mucosal surfaces of the respiratory, digestive and reproductive tracts and over the past few decades, the two main mechanisms used to control diseases have been the use of vaccines and antibiotics. However, in the poultry industry, there are mounting concerns over the ability of current vaccines to adequately protect against emerging hyper-virulent strains of pathogens and a lack of suitable, cost effective adjuvants. Thorough investigation of the immunogenetic responses involved in host-pathogen interactions will lead to the development of new and effective strategies for improving poultry health, food safety and the economic viability of the US poultry industry. In this paper, I describe the development of immunogenomic and proteomic tools to fundamentally determine and characterize the immunological mechanisms of the avian host to economically significant mucosal pathogens such as Eimeria. Recent completion of poultry genome sequencing and the development of several tissue-specific cDNA libraries in chickens are facilitating the rapid application of functional immunogenomics in the poultry disease research. Furthermore, research involving functional genomics, immunology and bioinformatics is providing novel insights into the processes of disease and immunity to microbial pathogens at mucosal surfaces. In this presentation, a new strategy of global gene expression using avian macrophage (AMM) to characterize the multiple pathways related to the variable immune responses of the host to Eimeria is described. This functional immunogenomics approach will increase current understanding of how mucosal immunity to infectious agents operates, and how it may be enhanced to enable the rational development of new and effective strategies against coccidiosis and other mucosal pathogens.

• 한국식품조리과학회지
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• 제21권1호
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• pp.40-46
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• 2005

본 연구에서는 식중독 유발세균에 대한 항균활성이 우수한 천연 항균성 물질을 검색하기 위해 예로부터 민간과 한방에서 널리 이용되어 온 청피를 각종 유기용매로 추출하여 식중독 유발세균에 대한 항균활성을 조사해 보았다. 청피를 petroleum ether, chloroform, ethly acetate, methanol를 이용하여 실온에서 각각 용매별로 계통 분획하고, 열수추출물을 얻은 후, 9종의 식중독 유발세균 (Staphylococcus-aureus, Bacillus cereus, Salmonella enteritidis, Shigella flexneri, Ercherichia coli. Salmonella typhimurium, Shigella dysenteriae, Pseudomonas aruginoia, Shigella sonnei) 에 대하여 항균효과를 조사하였다. 청피 추출물의 농도별 항균 활성 검색에서는 청피의 ethyl acetate 추출물이 가장 큰 항균 효과를 보였으며 Staphylococcus auren와 Pseudomonas aeruginosa가 가장 민감하게 반응하는 균주였다. 청피의 ethyl acetate 추출물과 백화사설초의 methanol 추출물을 혼합하여 항균력을 측정해 본 결과 두 추출물을 섞어 첨가했을 경우가 단독으로 사용했을 시 보다 상승효과를 나타내었다. 또한 청피의 ethyl acetate 추출물이 식중독 유발세균의 성장에 미치는 효과를 검정하기 위해 Bacillus cereus 및 Shigella sonnei의 배양액에 청피의 ethyl acetate 추출물온 각각 5,000 ppm 농도로 첨가했을 시, Bacillus cereus의 생육이 24시간 이상까지 억제됨을 관찰할 수 있었고, Shigella sonnei의 생육도 12시간까지 지연시킬 수 있었다.