• Title/Summary/Keyword: Food organisms

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Studies on the Food Additives. Effects of Condensates of Phosphates on the Preservative Activity of the Food Preservatives (식품첨가물에 관한 연구. 축합인산염이 식품보존료의 작용에 미치는 영향)

  • 문범수
    • YAKHAK HOEJI
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    • v.21 no.1
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    • pp.1-16
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    • 1977
  • The effect of condensates of phosphates on the preservative activity of the food additives, using eight micro-organisms, has been studied by the agar dilution method. It was found that condensates of phosphates increased the food preserving action of almost all of preservatives and they acted as a synergist for the action of almost all of preservatives. Disodium dihydrogen prrophosphate acted as the strongest synergist in the experiment. But sodium tripolyphosphate anhydrous, sodium poly-phosphate, sodium tripolyphosphate and sodium hexametaphosphate acted as a inhibitor for action of some preservatives on some orgnisms. The preservatives were sodium dehydroacetate (DHA), potassium sorbate and NaNO$_{2}$ etc. The action of sodium meta-phosphate on the preservatives produced a mean difference by their origins. It was supposed that such a difference was produced by the difference of amount of impurities that were contained in sodium metaphosphate, It was also supposed that the does of the preservatives could be reduced by addition of condensates of phosphates, acting as a synergist for the action of almost all of the preservatives.

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Circadian Rhythm and Metabolism in Mammals in the Aspects of Nutrition (영양 측면에서 본 포유동물의 하루 변동 특성과 대사(代謝) 리듬)

  • Park, Young-Sook;Mo, Su-Mi
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.13 no.2
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    • pp.222-228
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    • 1984
  • Since organisms have their rhythms by synchronizing the changes of periodic external circumstances and maintaining the integration of internal ones, circadian rhythm which affects to ingestion, digestion and metabolism of nutrients in the body, is important and interesting to discuss in the field of nutritional science. Also it is a serious public health problem tat various night-shift jobs are increasing now a days. Therefore authors wish to understand the properties of circadian rhythm and the rhythms of metabolic activities.

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The Effect of the SOD2 and SOD3 in Candida albicans on the Antioxidant System and its Potential as a Natural Antioxidant

  • Yeonju HONG;Min-Kyu KWAK
    • The Korean Journal of Food & Health Convergence
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    • v.10 no.2
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    • pp.13-17
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    • 2024
  • Oxygen is necessary to sustain life, but reactive oxygen species (ROS) produced by oxygen metabolism can cause mutations and toxicity. ROS can damage cellular macromolecules, leading to oxidative stress, which can accelerate cell death and aging. ROS generated in food affect the taste, color, and aroma of food, and high levels of ROS in meat can cause spoilage. Superoxide dismutase (SOD) plays an important role in scavenging ROS in food and reducing their toxicity to organisms. SOD exerts its antioxidant effect by catalyzing the breakdown of O2-• to H2O2. As a natural antioxidant, SOD has the ability to regenerate and maintain its activity over a long period of time without depletion, unlike chemical antioxidants that may have side effects or stability issues. This antioxidant effect of SOD has great potential in a variety of industries, and in the food industry it can be utilized to improve product quality and provide safe and healthy products to consumers. By disrupting the SOD2 and SOD3 genes in Candida albicans, we studied the effects of SOD2 and SOD3 genes on the antioxidant system, suggesting its potential as a natural antioxidant.

Comparative Evaluation on Qualitative PCR using Different Extraction Methods for Nucleic Acids on Soybean and Corn Processed Foods (대두 및 옥수수 가공식품에서 유전자재조합체(GMO)의 정성 PCR분석을 위한 핵산 추출방법별 비교)

  • 김영찬;이철수;황순욱;김성조;이영옥;윤성원;서정화;남용석
    • Journal of Food Hygiene and Safety
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    • v.18 no.1
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    • pp.6-13
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    • 2003
  • Various kinds of genetically modified organisms (GMO) and processed foods have been developed during recent years. Genetically modified organisms can be classified into several groups as their development methods. Generally, GMO has three foreign DNA regions such as gene expression adjustment region(Promoter), termination region (terminator) and structure gene. Detection of these regions can be done particularly by polymerase chain reaction (PCR). PCR-based detection can virtually be performed for any GMO within short of time. The most important prerequisite for the application of PCR-based detection is to decide abstraction method of efficient nucleic acids. Specially, in the case of processed food, because nucleic acids of foodstuffs are damaged by heat treatment (sterilization), pressure and fermentation, DNA must be extracted ken the samples prior to PCR analysis. Although many DNA extraction protocols are available, they have rarely been compared in a comprehensive method. In this study low widely used commercial and non-commercial DNA extraction methods-DNeasy$^{TM}$, Wizard$^{TM}$, CTAB, phenol/chloroform system-were compared with respect to the quality and yield of nucleic acids and insertion genes.nes.

Food and feeding activity of micronektonic fish, Gonostomatidae, from meso-and upper bathypelagic layers in the northeastern Atlantic (북동 대서양에서의 중심층 및 상부점심층산 앨퉁이과 어류 마이크로넥톤의 섭이양식)

  • 고유봉;쥴리안
    • 한국해양학회지
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    • v.21 no.2
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    • pp.85-91
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    • 1986
  • Diet and feeding patterns of 10 species of gonostomatid fish in the Rockall Trough and Porcupine Seabight, northeastern Atlantic, were investigated as a part of studies analyzing the meso-and upper bathypelagic micronektonic fish communities in these two areas. Copepods were the major food organisms, composing more than 72% in both areas, and ostracods the second most abundant prey organisms (〉13%). The results of stomach content analyses reveals that most of gonostomatids, including two important species, Cyclothone braueri and C. microdon, feed continuously regardless of light cycle and a little at a time. The gonostomatids appear to be planktivores that consume small-sized prey; relative size of most preys was about one-tenth of fish length. These feeding patterns could be an adaptive characteristic of fish living in great deep water where the food items are scarcely present. In general there are no considerable inter-and intraspecific differences, not to mention regional differences, in the feeding of the gonostomatids at both stations except the large specimen of Gonostoma elongatum fed fish. Thus several coexisting species of the gonostomatid fish utilize the same resources, showing competitive relationships.

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Microbial Quality and TBA Values of Chicken Patties as Affected by Irradiation and Storage Temperature (방사선 조사에 의한 닭고기 가공제품인 Patty의 미생물 및 TBA가 변화)

  • Chuang, J.T.;Yi, Y.H.;Chen, T.C.
    • Korean Journal of Food Science and Technology
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    • v.22 no.3
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    • pp.290-295
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    • 1990
  • prefried chicken patties were irradiated with doses of 0, 2, and 4kGy, and stored at $3{\pm}1^{\circ}C\;and\;-10{\pm}1^{\circ}C$, separately for the evaluation of microbiological quality and TBA values. Gamma irradiation increased (p<0.05) the shelf life of chicken patties stored at $3{\pm}1^{\circ}C$. The time required to reach a log number of 6.5/g was 22 days for the control, while 43 and 44 days for 2 and 4 kGy-irradiated samples, respectively. No microorganism was isolated from patties irradiated at 4 kGy. Irradiation decreased the incidence of gram-positive cocci-type organisms In patties ; while yeasts and gram-negative rod-type organisms dominated the microbial population isolated from 2 and 4 kGy-irradiated samples, respectively. TBA values were increased (p<0.05) as the irradiation dose increased and the storage time progressed.

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Safety Assessment of Genetically Modified Foods and Food Additives in Korea

  • Kim, Chang-Min
    • Toxicological Research
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    • v.17
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    • pp.185-188
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    • 2001
  • Genetically modified foods and food additives are derived from organisms that have been inserted foreign genetic materials by recombinant DNA techniques to improve the quality or any other pur-poses. The problems such as toxicity, allergenicity and antibiotics resistance in the safety of genetically modified foods are usually concerned. In Korea, the safety of foods is ensured by the Food Sanitation Act. Although there is no specific provision regarding the genetically modified foods in it, any foods that might cause negative effect(s) on public health or human life are prohibited to sell in the market. In order to systematically evaluate safety of genetically modified foods, the Korea Food and Drug Administration (KFDA) promulgated "Guidelines regarding review of safety assessment data for genetically modified foods and food additives (KFDA Notification 1999-46)". The objectives of these guidelines are to ensure safety of genetically modified foods and food additives. In order to evaluate the safety of genetically modified foods. KFDA operates a special expert committee composed by experts from government, universities, research institutes. and consumer's unions. Recently. manufacturers and consumers are interested in the issues on safety and labeling of genetically modified foods, because of increment of imported genetically modified crops and processed foods. Since government and consumers unions have different viewpoints, their positions regarding the issue are different each other. Therefore, the regulation of labeling on genetically modified foods is prepared and should be enforced at July 2000 in Korea. in Korea.

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Inhibitory Effect of Corn Silk Extract on Growth of Food-Borne Bacterial Pathogens

  • An, Eun-Sook;Kang, Sun-Hee;Chung, Hee-Jong
    • Food Science and Biotechnology
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    • v.15 no.1
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    • pp.138-142
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    • 2006
  • Various levels of antibacterial activity have been identified for water and ethanol extracts of corn silk, particularly against Salmonella typhimurium KCTC 2515. In general, the water extract was more effective than the ethanol extract. The minimum inhibitory concentration (MIC) for the water extract was 7.5 mg/disc for S. typhimurium KCTC 2515 and B. cereus KCTC 1092, as well as for the ethanol extract against S. typhimurium KCTC 2515 and S. typhimurium KCTC 1925. However, the MICs for the water extract were lower than those for the ethanol extract against all bacteria tested, except S. typhimurium KCTC 1925 and B. cereus KCTC 1014. The growth of the tested organisms in the synthesized broth medium was inhibited with the addition of 5-fold levels of MIC. Using sterilized milk as the model food system, we found that the lag phase for these microorganisms was extended up to 3 days at $20^{\circ}C$, but was not affected at $4^{\circ}C$. These results indicate that bacterial growth was strongly inhibited by corn silk extract at $20^{\circ}C$.

Detection of Genetically Modified Maize by Multiplex PCR Method

  • HEO , MUN-SEOK;KIM, JAE-HWAN;PARK, SUN-HEE;WOO, GUN-JO;KIM, HAE-YEONG
    • Journal of Microbiology and Biotechnology
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    • v.14 no.6
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    • pp.1150-1156
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    • 2004
  • The GMO (Genetically Modified Organism) labeling system on raw materials has been in Korea since March 2001, and genetically modified organisms (GMOs)-derived foods since July 2001. Therefore, we designed a multiplex PCR method to ascertain the validity of the labeling system and to monitor the status of circulation for genetically modified maize (GM Maize). Five lines of GM Maize (GA21, TC1507, Mon810, NK603, and Bt176) were used, and specific primer pairs were designed to detect each line. Using this method, the different lines of GM Maize were monitored from raw products and processed foods in Korean market. Some of the maize processed foods and raw materials were shown to contain more than one foreign gene. This method was found to be effective for-detecting five different GM Maize in a single reaction.

Comparison of Expression Pattern of Housekeeping Genes in Mice fed Genetically Modified Rice (유전자 이입에 따른 GM쌀 섭취 마우스의 Housekeeping Gene 발현 패턴 비교)

  • Lee, Dong-Yeob;Heo, Jin-Chul;Lee, Kyu-Hyun;Kim, Dong-Ho;U, Sang-Uk;Cho, Hyun-Suk;Lee, Sang-Han
    • Food Science and Preservation
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    • v.14 no.6
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    • pp.688-694
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    • 2007
  • To evaluate the human risk of long-term intake of genetically modified (GM) rice, we carried out RT-PCR of housekeeping genes. Housekeeping genes, which show highly uniform expression in living organisms during various stages of development and under different environmental conditions, were normalized by RT-PCR. We assessed the expression of 10 common housekeeping genes (18s rRNA, 25S rRNA, UBC, UBQ5, UBQ10, ACT11, GAPDH, eEF-$1{\alpha}$, ${\beta}$-TUB, GAPDH, ${\beta}$-actin, B2m, G6pd2, Gyk, Gus, Hprt, Cyclophlin A, Tfrc, ${\alpha}$-tubulin and RPL13A) in the liver, stomach, small intestine, large intestine, kidney and spleen of mice fed GM or non-GM rice. We found no significant differences in the expression of housekeeping genes between the two groups of mice.