• Journal of Korean Society of Occupational and Environmental Hygiene
• /
• v.24 no.4
• /
• pp.446-452
• /
• 2014

Objective: This study aimed to evaluate the exposure status of formaldehyde(FA) among the nurses in ambulatory care departments of university hospital. Methods: Two university hospitals were surveyed. The FA concentration in air were measured to target 62 nurses in 8 ambulatory care departments(89 samples). Air sampling and analysis of FA were carried out according to the OSHA Method ID-205. The survey was conducted with questionnaire asking about how to control FA and whether the nurses use the formaldehyde protectors or not. Results: FA was detected in all samples. The maximum concentration of FA was 0.258 ppm and the geometric mean was 0.023 ppm. There was no sample that exceeded any of exposure standards by OSHA-TWA whereas there were 54 samples(60.7%) that exceeded the standards by NOISH-TWA. Among 62 nurses handling FA, 13 nurses(21.0%) used the protective gloves while nobody used a gas mask. It was assessed that any of 8 common ambulatory care departments did not use a safety cabinet for FA in which local exhaust ventilation was fixed. Conclusions: Nurses in ambulatory care departments were exposed to FA. Therefore, the environment management of a workplace, the health management of a nurses, FA handling education and installing a FA cabinet with a local exhaust ventilation were needed because FA as a carcinogen was able to cause any cancer to a human body if it was emitted in air for long time.

• Journal of Life Science
• /
• v.21 no.10
• /
• pp.1364-1369
• /
• 2011

Mast cells, the central effector cells involved in the allergic response, release histamine, arachidonic acid, and proinflammatory cytokines. We investigated the effect of the ethyl acetate fraction (EA), derived from Korean rice wine lees, on RBL-2H3 cell degranulation and passive cutaneous anaphylaxis in an animal model. The EA fraction suppressed the release of beta-hexosaminidase, a marker of degranulation, and the mRNA expression of interleukin-3 (IL-3) and IL-13. EA also successfully suppressed the passive cutaneous anaphylaxis (PCA) reaction in mice in a dose-dependent manner. These results suggest that EA can inhibit mast cell degranulation through the inhibition of IL-3 and IL-13 mRNA expression, and that EA may potentially serve as an anti-allergic agent.

• Journal of Life Science
• /
• v.21 no.10
• /
• pp.1428-1433
• /
• 2011

In this study, we isolated and characterized plant-associated lactic acid bacteria which are able to produce angiotensin-converting enzyme (ACE) inhibitory and antioxidative activities. Five lactic acid bacteria were isolated from plants (grape and leek), a plant-associated fermentative product (Kimchi) and Korean traditional alcohol (Dongdongju). Strains K-1 and K-21 from Kimchi, strain L-5 from leek, strain G-3 from grape, and strain D-3 from Dongdongju were identified as Pediococcus pentosaceus, Lactobacillus plantarum, Weissella cibaria, L. plantarum, and L. brevis, respectively, by 16S rRNA gene analysis. ACE inhibitory activities of isolated strains ranged from 44.3 to 71.9% in the MRS broth. G-3, L-5 and K-1 strains especially showed high ACE inhibitory activities (59.8-98.69%) in the MRS broth containing skim milk. DPPH radical scavenging activities of the strains were in the range of 42.5-82.7%. All strains showed varying levels of resistance in artificial gastric fluid (pH 2.5), retaining viability ranging from 42.2 to 88.1% after 3 hr of incubation. All strains showed high resistance to 0.3% oxgall after 24 hr of incubation; survival rates were in the range of 55.4-112.8%. Isolated strains were found to be antagonistic to some pathogens including Pseudomonas aeruginosa.

• Journal of Life Science
• /
• v.22 no.4
• /
• pp.552-558
• /
• 2012

Severe acute respiratory syndrome (SARS) is a severe respiratory infectious disease caused by a novel human coronavirus, SARS-CoV. The 3CL protease is a key enzyme in the proteolytic processing of replicase polyprotein precursors, pp1a and pp1ab, which mediate all the functions required for viral genomic replication and transcription. Therefore, this enzyme is a target for the development of chemotherapeutic agents against SARS. A large quantity of active SARS-3CL protease is required for development of anti-SARS agents. Here we have constructed overexpression vector for the production of the SARS-3CL protease. The gene encoding SARS-3CL protease was amplified using polymerase chain reaction and cloned into the pET29a expression vector, resulting in pET29a/SARS-3CLP. Recombinant SARS-3CL protease was successfully synthesized by the dialysis mode of the cell-free protein expression system, and purified by three-step fast protein liquid chromatography using HighQ and MonoP column chromatographies and Sephacryl S-300 gel filtration. In addition, the produced SARS-3CL protease was found to be an active mature form. This study provides efficient methods not only for the development of anti-SARS materials from natural sources, but also for the study of basic properties of the SARS-3CL protease.

• Journal of Environmental Science International
• /
• v.22 no.11
• /
• pp.1389-1402
• /
• 2013

This paper focuses on the impacts of waste dumping on inorganic nutrients in the dumping area of the Yellow Sea, and the effect of an governmental regulation of pollution in dumping areas. The environmental variables and parameters of the dumping and reference areas in the Yellow Sea were measured during July 2009 and analyzed. In addition, the analyzed data for inorganic nutrients over the last 10 years were obtained from the Korea Coast Guard (KCG) and the National Fisheries Research and Development Institute (NFRDI). The chemical environment of the study area revealed increases in concentrations of inorganic nutrients, Chemical Oxygen Demand (COD), and Volatile Suspended Solids (VSS) in the bottom layer. On the contrary, the pH level was decreased. Most notably, the time series data of inorganic nutrients showed gradual increase over time in the dumping area, and thus, the oligotrophic waters trend toward eutrophic waters. The increases appears to be due to the disposal of large amounts of organic waste. In recent times, the wastes disposed at the area were largely comprised of livestock wastewater, and food processing waste water. The liquefied waste, which contains an abundance of nutrients, causes a sharp increase in concentrations of inorganic nitrogen in the dumping area. On the one hand, the dumping sites have been deteriorated to such an extent that pollution has become a social problem. Consequentially, the government had a regulatory policy for improvement of marine environmental since 2007 in the dumping area. Hence, the quality of marine water in the dumping site has improved.

• Korean Journal of Agricultural Science
• /
• v.30 no.2
• /
• pp.128-138
• /
• 2003

The bacterial density of partially processed horticultural crops in the commercial market was relatively high probably due to improper handing or processing. Between crops, the bacterial density was low in peeled garlic and ranged above $10^8cfu{\cdot}g^{-1}fw$ in other crops. Especially in brake fern, the bacterial density was the highest and non food materials such as packing strips were found. There was difference in the effects of ozone-treated water washing treatment between crops. In root crops such as burdock and lotus root, ozone showed positive effect on preventing discoloration as well as lowing bacterial density resulting in the increase of marketing period. Any positive effect was not found when sliced crops were treated over 5 min at $0.3mg{\cdot}L^{-1}$ of dissolved ozone. In lettuce slices, leaf tissues were severely discolored and rapidly soften at $0.3mg{\cdot}L^{-1}$ for 2 min dipping. Ozone was highly effective on inhibiting bacterium propagation and off-flavor. This effect was more stronger in Fischer ligulariata than brake fern, probably due to the difference of tissue thickness. Results indicated that ozone-treated water washing had a strong potential to improve the market quality of partially processed horticultural crops including dried wild greens and sliced root crops.

• Smart Media Journal
• /
• v.6 no.4
• /
• pp.58-64
• /
• 2017

Modern society is characterized by rapid increase in world population, aging of the rural population, decrease of cultivation area due to industrialization. The food problem is becoming an important issue with the farmers and becomes rural. Recently, the researches about the field of the smart farm are actively carried out to increase the profit of the rural area. The existing smart farm researches mainly monitor the cultivation environment of the crops in the greenhouse, another way like in the case of poor quality t is being studied that the system to control cultivation environmental factors is automatically activated to keep the cultivation environment of crops in optimum conditions. The researches focus on the crops cultivated indoors, and there are not many studies applied to the cultivation environment of crops grown outside. In this paper, we propose a method to improve the harvestability of poor areas by monitoring the areas with bad harvests by using big data analysis, by precisely predicting the harvest timing of fruit trees growing in orchards. Factors besides for harvesting include fruit color information and fruit weight information We suggest that a harvest correlation factor data collected in real time. It is analyzed using the Apache Spark engine. The Apache Spark engine has excellent performance in real-time data analysis as well as high capacity batch data analysis. User device receiving service supports PC user and smartphone users. A sensing data receiving device purpose Arduino, because it requires only simple processing to receive a sensed data and transmit it to the server. It regulates a harvest time of fruit which produces a good quality fruit, it is needful to determine a poor harvest area or concentrate a bad area. In this paper, we also present an architectural model to determine the bad areas of fruit harvest using strong data analysis.

• Applied Biological Chemistry
• /
• v.33 no.4
• /
• pp.325-329
• /
• 1990

This study was undertaken to determine thermodynamic characteristics of B. subtilis p-4 and B. licheniformis p-5 proteases isolated from fermented anchovy paste. $K_m$ values of two proteases for casein as a substrate were 0.38mM in p-4 protease and 0.18mM in p-5 protease, respectively. Denaturation constants($K_D$) of p-4 and p-5 proteases were $12.2{\times}10^{-5}/sec\;and\;19.0{\times}10^{-5}/sec\;at\;40^{\circ}C,\;and\;35.7{\times}10^{-5}/sec\;and\;46.3{\times}10^{-5}/sec\;at\;50^{\circ}C$, respectively. Activation energies($E_a$) of p-4 and p-S pmteases were 19.6 Kcal/mole and 15.2kcal/mole, respectively. Free energy of activation(${\Delta}G^*$), activation enthalpy(${\Delta}H^*$) and activation entropy(${\Delta}S^*$) at $40^{\circ}C$ were 23.21Kcal/mole, 18.98Kcal/mole and -13.50 eu, respectively for p-4 protease and 22.93Kcal/mo1e, 14.58Kcal/mole and -26.68 eu, respectively for p-5 protease. The major amino acids in p-4 protease(151 residues of amino acid) were Gly, Glu, Pro, Asp, Ser, Ala, Lys and Leu, while those in p-5 protease(247 residues of amino acid) were Gly, Glu, Asp, Ala and Leu. It may be concluded that heat denaturation of two proteases showed liner regression curve and p-5 protease was more sensitive to heat than p-4 protease.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.34 no.3
• /
• pp.532-542
• /
• 2007

The purpose of study was to review the transition of dentition according to the evolution of man to know the background of the dental problems like hypodontia and malocclusion. Man is Kingdom Animalia, Phylum Chordata, Class Mammalia, Order Primates, Suborder Haplorrhini, Superfamily Hominoidea, Family Hominidae, Genus Homo, Species Sapiens by taxonomy. The first hominid was Australopithecus which appeared c. 4 millions of years ago and showed bipedalism and distinct dentition. Homos began with H. habilis who appeared c. 2.5 millions of years ago and made stone tools, and then H. erectus and H. neanderthalensis appeared and disappeared until H. sapiens came. The dental formula of primitive mammalians which was I3 C1 P4 M3 changed to I2 C1 P4 M3 of primitive primates, to I2 C1 P3 M3 of Haplorrhini, and to I2 C1 P2 M3 of hominoids. That of H. sapiens is changing to I2 C1 P2 M2.The box type dentition of hominoids changed to the omega type dentition of Australopithecus, and to the parabolic type of H. sapiens. The size of teeth decreased continually, especially the canine and sexual dimorphism. The dentition moved backward and downward to the cranial crown according to the increase of the brain and decrease of the jaws. It was suggested that the change of diet to the starchy foods, food processing, and the development of cooking reduced the necessity of mastication and caused the change of dentition. The future of H. sapiens who is quite a new species in the earth histroy and is now causing the mass extinction of other species is hard to see. It seems that hypodontia and malocclusion are related to the dentition change according to the evolution of man and is likely to increase.

• Culinary science and hospitality research
• /
• v.18 no.2
• /
• pp.91-105
• /
• 2012

This study aims to analyze the effects of satisfaction on behavioral intentions depending on brand equity based on the consumers who visited specialty coffee shops in Busan. The self-administered method was used for empirical analysis and 295 copies (89%) out of 300 copies of questionnaires were used for analysis. For data processing, the SPSS WIN Version 15.0 statistical package program was used and the frequency analysis, reliability analysis, factor analysis were conducted so the hypothesis was verified through the regression analysis. As a result, for the hypothesis that "Brand equity(perceived quality, brand awareness, brand image) will have a significant impact on satisfaction," perceived quality, brand awareness, and brand image had a significant impact on satisfaction. Satisfaction was shown to have a significant impact on behavioral intentions for the hypothesis that "Satisfaction will have a significant impact on behavioral intentions" For the hypothesis that "Brand equity(perceived quality, brand awareness, brand image) will have a significant impact on behavior intentions," perceived quality and brand awareness had a significant impact on behavioral intentions while brand image didn't. Based on the results of this study, companies should be systematically managed to prepare strategies to remind consumers of their brands and focus on to strengthen brand equity effectively in order to build a competitive brand.