• 대한의생명과학회지
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• 제29권4호
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• pp.296-301
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• 2023

This study was conducted to induce muscle loss using dexametasone and then use the extract to determine its effectiveness in a muscle loss animal model. Animal experimental groups were divided by five groups. Changes in the weight of the animals were measured for a total of 5 weeks. After animal sacrifice, muscle mass was measured, and animal behavior evaluation was conducted using grip strength test and pole test. The expression levels of MAFbx protein was measured using muscle samples. Oral administration of Fomes fomentarius extract was effective in suppressing muscle atropy and increasing muscle, which was confirmed through animal behavior evaluation and muscle-related protein expression.

• Mycobiology
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• 제43권4호
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• pp.450-457
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• 2015

Medicinal mushrooms have been used worldwide to treat cancer and modulate the immune system. Over the last several years, there has been increasing interest in isolating bioactive compounds from medicinal mushrooms and evaluating their health beneficial effects. Fomes fomentarius is used in traditional oriental medicine and is known to possess antioxidant, antiinflammatory, antidiabetic, and antitumor effects. In the present study, we isolated fomentariol from Fomes fomentarius and investigated its anti-inflammatory effect in murine macrophages (RAW264.7 cells) stimulated with lipopolysaccharides. Fomentariol inhibited the production of nitric oxide and intracellular reactive oxygen species triggered by lipopolysaccharides. Interestingly, fomentariol differentially regulated cytokine production triggered by lipopolysaccharides. Fomentariol effectively suppressed the production of interleukin-$1{\beta}$ and interleukin-6 but not tumor necrosis factor-${\alpha}$. The inhibitory effect of fomentariol against nitric oxide, interleukin-$1{\beta}$, and interleukin-6 production was possibly mediated by downregulation of the extracellular signal-regulated kinase signaling pathway. Taken together, our results suggest that fomentariol differentially modulated inflammatory responses triggered by lipopolysaccharides in macrophages and is one of the bioactive compounds that mediate the physiological effects of Fomes fomentarius.

• Mycobiology
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• 제43권3호
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• pp.319-326
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• 2015

Fomes fomentarius is a fungus of the Polyporaceae family and is used in traditional oriental therapies. Although the anti-inflammatory activities of this species have been previously reported, the identity of the bioactive compounds responsible for this activity remains unknown. Here, we investigated whether methyl 9-oxo-(10E,12E)-octadecadienoate (FF-8) purified from F. fomentarius exerts anti-inflammatory activity in murine macrophages stimulated with lipopolysaccharide (LPS). FF-8 suppressed secretion of nitric oxide (NO) and prostaglandin $E_2$ through downregulation of inducible NO synthase and cyclooxygenase-2 expression induced by LPS. In addition, pretreatment of cells with FF-8 led to a reduction in levels of secreted inflammatory cytokines such as tumor necrosis factor-${\alpha}$ and interleukin-6 in macrophages stimulated with LPS. Conversely, FF-8 did not affect nuclear factor ${\kappa}B$, p38, c-Jun NH2-terminal kinase, and extracellular signal-regulated kinase pathways. Instead, FF-8 specifically interfered with signal transducer and activator of transcription 3 (STAT3) phosphorylation induced by LPS. Collectively, this study demonstrated that FF-8 purified from F. fomentarius suppresses inflammatory responses in macrophages stimulated with LPS by inhibiting STAT3 activation. Further studies will be required to elucidate the anti-inflammatory effect of FF-8 in vivo.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.147.1-147.1
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• 2003

As an attempt to search for bioactive natural products exerting antiinflammatory activity, we have evaluated the anti-inflammatory and antinociceptive activities of the methanol extract from the Fomes fomentarius (MEFF). MEFF (50, 100 mg/kg/day, p.o.) significantly reduced an acute paw edema induced by carrageenan in rats. When analgesic activity was measured by acetic acid-induced writhing test and hot plate test, MEFF showed a dose-dependent inhibition in animal models. In addition, MEFF potently inhibited the LPS-induced production of NO, $PGE_2$ and TNF-$\alpha$production of macrophages. (omitted)

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2004년도 Annual Meeting BioExibition International Symposium
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• pp.316-320
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• 2004

• 한국식품영양과학회지
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• 제34권6호
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• pp.825-832
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• 2005

말굽버섯(Fomes fomentarius)이 당뇨에 미치는 영향을 연구할 목적으로 실험동물에 STZ를 투여하여 당뇨를 유발시킨 후 말굽버섯 물추출물과 메탄을 추출물을 2주간 투여하여 혈당,지질함량 및 면역에 미치는 영향을 검토한 결과는 다음과 같다. 혈당은 당뇨로 인해 증가하였으나 말굽버섯 추출물 투여군에서 유의적으로 감소를 보였다. 혈청과 간의 총콜레스테롤과 중성지방 함량은 말굽버섯 추출물 급여로 DM군에 비하여 현저한 감소를 나타내었다. HDL-콜레스테롤 함량과 GSH-Px의 활성은 말굽버섯 추출물 급여 시 에는 DM군과 비교하여 유의한 증가가 나타났다. 혈청 보체 C3의 함량은 대조군에 비하여 DM군에서 유의한 감소를 보였고, DM-FM 200군에서 대조군 수준으로 회복이 관찰되었다. B cell과 T cell은 DM군에서 유의한 감소를 보였고, 말굽버섯 추출물 투여로 유의적으로 증가되었다. Helper T cell과 suppressor T cell의 분포는 DM군에서 유의하게 감소되었으나, 말굽버섯 추출물 투여로 유의한 증가를 나타내었다. 이상의 결과를 종합하여 볼 때 말굽버섯의 물 추출물과 메탄올 추출물은 혈당조절 기능, 지질대사 개선 및 면역작용을 활성화시킴으로서 STZ의 투여로 인한 당뇨의 증상을 완화시키는 것으로 사료된다.

• 한국영양학회:학술대회논문집
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• 한국영양학회 2003년도 연합학술대회
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• pp.250.2-250.2
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• 2003

• Journal of Applied Biological Chemistry
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• 제57권4호
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• pp.373-377
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• 2014

본 연구에서는 아직 까지 연구되지 않은 말굽버섯의 면역증강 효과를 설치류 대식세포에서 검증하였다. 연구결과에 따르면 말굽버섯 메탄올 추출물(FFE)의 처리에 의해 대식세포의 산화질소의 생성이 농도 의존적으로 증가되었다. 산화질소의 생성이 증가된 이유는 산호질소의 생성을 유도하는 효소인 iNOS의 발현이 FFE에 의해 증가되었기 때문이었다. FFE는 면역반응에 중요한 cytokine인 TNF-${\alpha}$, IL-$1{\beta}$, IL-6의 생성도 농도 의존적으로 증가 시켰다. 이 같은 FFE의 면역증강 활성은 면역활성을 중계하는 세포 신호전달분자 중 NF-${\kappa}B$와 MAP Kinases의 활성증가에 의한 것임을 확인 할 수 있었다. 본 연구 결과는 말굽버섯의 임상적 적용에 중요한 자료로 활용될 것이며, 만일 말굽버섯으로부터 분리한 단일성분에서 면역증강활성을 검증 하게 된다면 새로운 식 의약소재로서의 가치를 인정 받을 수 있을 것으로 생각된다.

• Journal of Microbiology
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• 제44권1호
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• pp.29-34
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• 2006

The purpose of this study was to develop molecular identification method for medical mushrooms and their preparations based on the nucleotide sequences of nuclear large subunit (LSD) rDNA. Four specimens were collected of each of the three representative medicinal mushrooms used in Korea: Ganoderma Incidum, Coriolus versicolor, and Fomes fomentarius. Fungal material used in these experiments included two different mycelial cultures and two different fruiting bodies from wild or cultivated mushrooms. The genomic DNA of mushrooms were extracted and 3 nuclear LSU rDNA fragments were amplified: set 1 for the 1.1-kb DNA fragment in the upstream region, set 2 for the 1.2-kb fragment in the middle, and set 3 for the 1.3-kb fragment downstream. The amplified gene products of nuclear large subunit rDNA from 3 different mushrooms were cloned into E. coli vector and subjected to nucleotide sequence determination. The sequence thus determined revealed that the gene sequences of the same medicinal mushroom species were more than $99.48\%$ homologous, and the consensus sequences of 3 different medicinal mushrooms were more than $97.80\%$ homologous. Restriction analysis revealed no useful restriction sites for 6-bp recognition enzymes for distinguishing the 3 sequences from one another, but some distinctive restriction patterns were recognized by the 4-bp recognition enzymes AccII and HhaI. This analysis was also confirmed by PCR-RFLP experiments on medicinal mushrooms.

• 한국버섯학회지
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• 제20권3호
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• pp.163-167
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• 2022

본 연구에서는 균막 형성을 위해 기존에 활용하고 있는 버섯 종과 다른 말굽버섯을 활용하였으며, 다양한 조건으로 감마선 조사를 진행하여 독립성이 검증된 변이균주를 획득하였다. 확보된 균주들의 톱밥배지 및 액체배지에서의 균막 형성을 관찰하고 각 균막의 특성을 조사한 결과 장수버섯, 시루송편버섯의 균막과 비교하여 비슷하거나 더 좋은 결과를 보인 균주를 확인할 수 있었다. 또한 톱밥배지보다는 액체배지에서 배양 했을 때 균막의 활용도 및 경제성이 더 높은 것으로 판단된다. 본 결과는 버섯 균사체를 이용한 대체육, 바이오소재 발굴을 위한 연구에 활용할 수 있을 것으로 여겨진다.