• Title/Summary/Keyword: Foliar treatment

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Paromomycin Derived from Streptomyces sp. AG-P 1441 Induces Resistance against Two Major Pathogens of Chili Pepper

  • Balaraju, Kotnala;Kim, Chang-Jin;Park, Dong-Jin;Nam, Ki-Woong;Zhang, Kecheng;Sang, Mee Kyung;Park, Kyungseok
    • Journal of Microbiology and Biotechnology
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    • v.26 no.9
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    • pp.1542-1550
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    • 2016
  • This is the first report that paromomycin, an antibiotic derived from Streptomyces sp. AG-P 1441 (AG-P 1441), controlled Phytophthora blight and soft rot diseases caused by Phytophthora capsici and Pectobacterium carotovorum, respectively, in chili pepper (Capsicum annum L.). Chili pepper plants treated with paromomycin by foliar spray or soil drenching 7 days prior to inoculation with P. capsici zoospores showed significant (p < 0.05) reduction in disease severity (%) when compared with untreated control plants. The disease severity of Phytophthora blight was recorded as 8% and 50% for foliar spray and soil drench, respectively, at 1.0 ppm of paromomycin, compared with untreated control, where disease severity was 83% and 100% by foliar spray and soil drench, respectively. A greater reduction of soft rot lesion areas per leaf disk was observed in treated plants using paromomycin (1.0 μg/ml) by infiltration or soil drench in comparison with untreated control plants. Paromomycin treatment did not negatively affect the growth of chili pepper. Furthermore, the treatment slightly promoted growth; this growth was supported by increased chlorophyll content in paromomycin-treated chili pepper plants. Additionally, paromomycin likely induced resistance as confirmed by the expression of pathogenesis-related (PR) genes: PR-1, β-1,3-glucanase, chitinase, PR-4, peroxidase, and PR-10, which enhanced plant defense against P. capsici in chili pepper. This finding indicates that AG-P 1441 plays a role in pathogen resistance upon the activation of defense genes, by secretion of the plant resistance elicitor, paromomycin.

Enhancing Effect of Shimizuomyces paradoxus on Seed Germination and Seedling Growth of Canola, Plant Growth of Cucumber, and Harvest of Tomato

  • Sung, Gi-Ho;Shrestha, Bhushan;Park, Ki-Byung;Han, Sang-Kuk;Sung, Jae-Mo
    • Mycobiology
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    • v.39 no.1
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    • pp.7-11
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    • 2011
  • Shimizuomyces paradoxus showed no inhibitory effect against plant pathogen fungi, such as Fusarium oxysporum f. sp. lycopersici and Alternaria solani. The S. paradoxus culture filtrate showed higher seed germination and seedling growth rates in canola than distilled water and potato-dextrose broth. A conidial suspension of $1.0{\times}10^4/mL$ resulted in the highest growth stimulating effects on total plant length, and fresh and dry weight of shoots and roots in cucumber, when compared to the highest suspension concentration. Total plant length and shoot weight increased with the foliar spray treatment, and root length and root weight increased by simultaneous treatments of soil drenching and foliar spray in cucumber. Lower concentrations of the S. paradoxus conidial suspension increased the harvest of tomato fruit.

Lateral Bud Suppression and Runner Plants Growth of 'Maehyang' Strawberry as Affected by Application Method and Concentration of IBA (IBA 처리방법과 농도에 따른 딸기 '매향'의 측아 발생 억제와 자묘 생육)

  • Hwang, Hee Sung;Jeong, Hyeon Woo;Lee, Hye Ri;Hwang, Seung Jae
    • Journal of Bio-Environment Control
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    • v.29 no.1
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    • pp.80-88
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    • 2020
  • This study was conducted to examine the effect by application method and concentration of the indole-3-butyric acid (IBA), which is auxin-based plant growth regulator, on the growth and runner plants production of strawberry in the greenhouse. The seedlings of strawberry were transplanted in the pot (150 ×135 × 90 mm) filled with coir medium on April 12, 2019. The IBA was applied with a foliar spray or drench as 50, 100, 150, and 200 mg·L-1 (50 mL per plant), respectively. The treatment was started on April 29, 2019. The foliar spray and drench treatment of IBA were repeated at 2-week intervals for 9 weeks from the start date of treatment. At 9 weeks after treatment, the petiole length of mother plants was the shortest in the control. The number of runner plants showed a tendency to decreased in the foliar spray. The number of lateral buds showed a tendency to decreased in the IBA treatment, and the least in the foliar with 100 mg·L-1. There was not significantly difference in the fresh and dry weights of the first and second runner plants. However, in the third runner plants, the fresh and dry weights were the greatest in the drench with 100 mg·L-1. Therefore, when considering the growth of third runner plants and lateral bud suppression, the drench with the 100 mg·L-1 could be better application method and concentration of IBA treatment for growth of the third runner plants and runner plants production of strawberry, and the results can be used as a basic research of plant growth regulator application to save the labor force and enhance the seedling quality in strawberry seedling stage.

Effects of Foliar Application of Ethychlozate Mixed with Calcium Formulae on a Fruit Quality of Satsuma Mandarin ('Miyagawa Wase') in Plastic Film House Cultivation (Ethychlozate와 Ca제제 혼용살포가 하우스밀감의 품질에 미치는 영향)

  • Kim, Yong Ho;Moon, Duck Young;Kim, Han Yong
    • Horticultural Science & Technology
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    • v.18 no.5
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    • pp.605-611
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    • 2000
  • This experiment was conducted to examine the effects of foliar application of ethychlozate and ethyclozate mixed with different calcium formulae (clef-non, suical, cell-bine, and calcium acetate monohydrate) on the fruit quality and peel puffing of 'Miyagawa Wase' satsuma mandarin cultivated in a plastic film house. Foliar application of ethychlozate mixed with clef-non or suical showed a result that the 'a' value of peel chromaticity was increased, which are supposed to accelerate peel coloration without peel puffing. The reducing sugar levels of fruits in control, ethychlozate, ethychlozate+celef-non, ethychlozate+suical, ethychlozate+cell-bine, and ethychlozate+calcium acetate monohydrate treatment were 4.98, 5.30, 5.59, 5.00, 5.20, and 4.27%, respectively. Especially, in the case of ethychlozate mixed with clef-non, the reducing sugar level was 0.61% higher than that of control. Sucrose and total sugar content also had a similar trend as that in the reducing sugar contents. The sugar contents of fruits in various ethychlozate treatments mixed with different calcium formulae except those in ethychlozate treatment or ethychlozate treatment mixed with calcium acetate monohydrate were higher than $12^{\circ}Brix$. Especially, the treatment of ethychlozate treatment mixed with clef-non showed the highest sugar content with $12.7^{\circ}Brix$. The ratio of soluble solids to acidity also showed the similar tendency, but there was no significant difference in acidity among the treatments.

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Use of Sprinkler System for Control of the Pine Needle Gall Midge, Thecodiplosis japonensis Uchida et Inouye (분무장치(噴霧裝置)를 이용(利用)한 솔잎혹파리 방제(防除)에 관(關)한 연구(硏究))

  • Chung, Sang Bae;Kim, Chul Su
    • Journal of Korean Society of Forest Science
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    • v.83 no.3
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    • pp.311-321
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    • 1994
  • Experiments were conducted to evaluate the efficacy of ground and foliar sprinkling of natural water for control of the pine needle gall midge, Thecodiplosis japonensis, by disturbing the reproductive behavior of adults such as copulation and oviposition. Diurnal ground spray for whole area application and foliar spray for single tree treatment were tested at day time during the period of adult emergence. The results obtained are as follows : 1. The population density of overwintering larvae in soil was not affected by the water spray during the adult emergence period. 2. Spraying the water showed no effect on the whole period and number of adult emergence, but retarded early emergence and facilitated Late emergence. The difference in the emerging time by 50% emergence day was approximately 7 days. 3. Ground spray was significantly different from control in gall formation rates but was not sufficient for effective control. As a single-tree treatment, the foliar spray was found to be highly effective for control of the pine needle gall midge, Average gall formation of spray plots and untreated ones were 2.6% and 38%, respectively ; control value exceeded 90%. 4. Ground spray of water was not effective in reducing larvae in gall of needle. Foliar spray, however, reduced 45% of larval numbers. 5. Foliar spray accelerated shoot growth by 15%-18%. 6. Foliar spray was highly effective for the control of sucking insects, such as mites and aphids on pine trees.

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Effects of Foliar Application of CaCl2 on Ca Content and Occurrence and Alleviation of Blossom-end Rot of Paprika During High Temperature Season (고온기 CaCl2 엽면 살포가 파프리카 칼슘함량, 배꼽썩음과 발생과 경감에 미치는 영향)

  • Oh, Jeong Sim;Lee, Yong-Beom;Bae, Jong Hyang;Na, Jong Kuk;Choi, Ki Young
    • Journal of Bio-Environment Control
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    • v.30 no.4
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    • pp.263-270
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    • 2021
  • This experiment was carried out to determine the effect of CaCl2 foliar spraying on the inhibition of blossom-end rot (BER) in hot summer paprika (Capsicum annum L. 'Special') cultivation. The effect of CaCl2 application was examined by the foliar application based on different fruit size, frequency, and spraying time. Also, it was investigated the occurrence and alleviation effect of BER symptom. Foliar application of CaCl2 (Ca 0.4%) was conducted by treating a fruit and leaf at 4 to 9 nodes above the crown flowers of each plant with 350 mL per week from June 3 to July 1. When the CaCl2 was sprayed at 7-day intervals for 4 weeks, the Ca content was the lowest in the fruit harvested with BER symptom in 11 to 20 mm of fruit width (FW). Four different regions in both BER symptom and normal fruits showed significant differences of Ca content, the highest was in pedicel, followed by stem-end, middle, and blossom-end. The Ca content increased sharply in normal paprika with 31-40 mm FW, in which Ca content was 78% higher than that of BER. Ca content in the middle and blossom-end of paprika over 21 mm in FW ranged 19.8% to 28.8% in normal fruits and 15.7% to 18.5% in BER, respectively. The incidence of BER increased rapidly by more than 60% in fruits with 31-40 mm FW. While there was no difference in fruit weight among the FW treatment, marketable yield rate was highest in the 21-30 mm FW, and the sugar content was high in the 11-30mm FW. When CaCl2 was applied three times for 7 days to a paprika having a 21- 30 mm FW, the cell wall-bound (CWB) Ca content was the highest and the rate of BER was lowest with 6.3%. After 10 days of CaCl2 foliar spray treatment, the CWB Ca content of paprika increased by 2.9 to 3.5 times compared to the control in all treatments. At 7 days after the CaCl2 foliar spraying once a day at varying spraying time, the leaf burn observed from 9:30 a.m. to 17:00 p.m. and the proline content increased as the spraying time was delayed. Therefore, the CaCl2 foliar spraying method for reducing of BER occurrence during paprika summer cultivation seems to be appropriate to spray 2-3 times at intervals of 3 days and before 8 a.m. at the time when the FW is 21-30 mm.

Comparing of Clonal Sensitivity of Populus deltoides to Atmospheric Ozone with Use of Visible Foliar Injury (잎의 가시적(可視的) 피해(被害)에 따른 오존에 대(對)한 미류나무(Populus deltoides) 클론간(間) 감수성(感受性) 비교(比較))

  • Lee, Jae-Cheon;Kim, In-Sik;Yeo, Jin-Kie;Koo, Yeong-Bon
    • Journal of Korean Society of Forest Science
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    • v.90 no.1
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    • pp.10-18
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    • 2001
  • Experiments were conducted to compare ozone sensitivity among clones. Ten clones of Papulus deltoides Marsh. were exposured in walk-in type chambers to charcoal-filtered air, 50ppb, 100ppb, and 150ppb ozone for 8h $day^{-1}$ for 21 consecutive days. Occurrence of premature leaf-fall, and visible foliar injury expressed as adaxial stipple were measured after termination of ozone exposure for 3 weeks. Rate of premature leaf-fall increased progressively according to ozone exposure levels. As a result, rate of premature leaf-fall was estimated over 50% at 150ppb ozone. In the charcoal-filtered air and 50ppb ozone treatments, visible foliar injury was not found. But injury was estimated as LA; 17.3%, AA; 6.5%, and LAA; 1.6% to 100ppb ozone treatment and LA; 34.1%, AA; 17.5%, and LAA; 7.4% to 150ppb ozone treatment. Clonal differences of sensitivity within the species were manifested by significant clone differences of adaxial stipple(LAA) in 100ppb and 150ppb ozone treatments.

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Study on the Response of Korean Ginseng (Panax ginseng C.A. Meyer) to the Herbicide 2,4-D Application (고려인삼에 대한 제초제 2, 4-D의 반응연구)

  • Jo, Jae-Seong
    • Journal of Ginseng Research
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    • v.14 no.2
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    • pp.291-296
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    • 1990
  • Various rates of 2,4-D were sprayed on 2 and 3 year old ginseng plants as foliar spray to define the critical concentration. No apparent plant injury was noticable for those ginseng plants when application concentration of 2,4-D doubled the recommended dosage (70 ml/10a). Neither abnormal foliar change occurred nor any inhibition in leaf and stem growth was resulted for the plants treated with 2,4-D concentrated two times of the recommended dosage. When the rates of 2,4-D application were increased greater than this level, injury ratings increased linearly with the rates of 2,4-D application and plant you was inhibited. Ethylene gas was not produced from the ginseng plant treated with 2 times concentrated 2,4-D, however the ginseng plants produced 0.03 to 0.09 ppm ethylene gas when the rate of application were increased 3 and 4 times, respectively. On the other hand the soybean treated with the recommended amount of 21-D produced ethylene gas of 10-20 times higher compared with ginseng plants and died. Photosynthesis ability of the ginseng leaf was significantly decreased by 2,4-D foliar application but it was recovered 4 weeks after 2,4-D foliar treatment. The herbicide 2,4-D was applicated to 2,3 and 4 years old ginseng plants as foliar spray with the rates of 0.5, 1.0, 1.5 and 2.0 times of the recommended dosage to define the effects of 2,4-D on the plant growth and root yield of the ginseng. There were no significant differences in the leaf and stem growth between untreated and 2,4-D treated plant. Berry maturing of 3 and 4 year old ginseng was not influenced by 2,4-D. The root weight of 4 years old ginseng plant was not reduced by application of 2,4-D concenrated 2 times of the recommended dosage, Application time of the herbicide 2,4-D had no effects on the leaf or stem growth of 2,3 and 4 year old year old ginseng plants. When the ginseng seedling was treated with 2,4-D, detrimental phenomena as stem bending and docoration of seedling leaf margin occurred, but stem bending was recovered in a few day s. Keywords Panax ginseng C.A. Meyer, 2,4-D , herbicide.

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Factors Affecting Boron Deficiency Symptoms in Spring Grown Radishes(Raphanus sativus L.) (봄무우 붕소결핍증(硼素缺乏症)의 발생생리(發生生理)에 관(關)한 연구(硏究))

  • Hur, Il-Bong;Kim, Moo-Sung;Hwang, Son-Koo;Ryu, In-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.16 no.1
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    • pp.72-76
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    • 1983
  • Absorption pattern and sensitivity of radishes to boron were compared with different boron treatment: soil application, foliar spray and no boron application. Six radish cultivars were planted on April 7 and harvested on June 23. Borax 1.5 Kg per 10 a was applied to soil before seeding and the 150 litters of 0.5 ppm solution with bolic acid was sprayed on leaves 3 times during the growing period. Boron content in plant was markedly increased by the soil application but no significant difference in boron contents in plants was observed between foliar spray and no application, presumably because of the insufficient dose of foliar spray. The darkening rot in root, a boron deficiency, was negligible in radishes grown in soil application plots whereas a relatively higher percentage of boron deficient plant was found from foliar spray or no application plots. The appearance of root darkening rot occurred at the rapid root enlargement stage, apparently from 55 days to 65 days after seeding. The boron contents in plants of soil application plots slightly increased at this stage while those of no boron application and foliar spray decreased. This obviously verified that boron deficiency should cause root rots showing highly significant relationship.

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