• Journal of the Computational Structural Engineering Institute of Korea
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• v.36 no.4
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• pp.213-220
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• 2023

This paper presents a model simulating the folding process and collision dynamics of "ddakji", a traditional Korean game played using paper tiles (which are also referred to as ddakji). The model uses two A4 sheets as the base materials for ddakji. The folding process involves a series of boundary conditions that transform the wing part of the paper structure into a twisted configuration. A rigid plate boundary condition is also adopted for squeezing, establishing the shape and stress state of the game-ready ddakji through dynamic relaxation analysis. The gaming process analysis involves a forced displacement of the striking ddakji to a predetermined collision position. Collision analysis then follows at a given speed, with the objective of overturning the struck ddakji--a winning condition. A genetic algorithm-based optimization analysis identifies the optimal collision conditions that result in the overturning of the struck ddakji. For efficiency, the collision analysis is divided into two stages, with the second stage carried out only if the first stage predicts a possible overturn. The fitness function for the genetic algorithm during the first stage is the direction cosine of the struck ddakji, whereas in the second stage, it is the inverse of the speed, thus targeting the lowest overall collision speed. Consequently, this analysis provides optimal collision conditions for various compression thicknesses.

• Journal of the Korea Furniture Society
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• v.28 no.4
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• pp.259-267
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• 2017

Furniture should change according to space. Therefore, it is important that the flexibility of the furniture, the functional efficiency, the economical efficiency and the convenience of the furniture of today. These characteristics are also important for seminar tables that are used temporarily by a large number of people. The seminar furniture currently in use has no shielding plate, there are two forms of table and shielding plate folding table. Therefore, in this research, furniture design and product development that can complement the shortcomings of the seminar furniture currently in use were done. Analysis of problems through overseas investigation and domestic market research, design planning and design, and prototyping. We have sought to optimize the volume by applying the characteristics of RTA furniture to develop a lightweight, lightweight, lightweight, easy to install and easy to use shielding plate, and open and closed system. Basically developed two types of shielding board seminar table basic shape and Roll Screen type, we expanded the scope of application such as each color and pattern. In order to reduce the weight, we replaced the parts such as anchor bolts and hinges and the shielding plate with Roll Screen and pursued volume optimization. As a result of this research, it is expected that the system of mechanisms such as packaging standardization and damage prevention for weight reduction and volume optimization will contribute to building competitiveness by domestic office prefabricated furniture industry's original technology.

• Journal of Intelligence and Information Systems
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• v.6 no.2
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• pp.41-47
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• 2000

Ideograph is a truly unifies data and procedural dependencies. Ideograph can be used to assist various program optimization, such as common expression elimination, code motion, constant folding etc. In this paper, we propose an improved representation of the data and control flow dependencies information for the efficient program execution. In pursuing this goal, we propose a model and in particularly implement a dependency information extractor and information table, which contains data and control flow information per a basic block And then we design and implementation of the optimized abstract syntax tree using Ideograph which has a control flow information and data flow information for source program.

• International Journal of Control, Automation, and Systems
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• v.4 no.3
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• pp.382-393
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• 2006

Mechanical system dynamics plays an important role in the area of computational structural biology. Elastic network models (ENMs) for macromolecules (e.g., polymers, proteins, and nucleic acids such as DNA and RNA) have been developed to understand the relationship between their structure and biological function. For example. a protein, which is basically a folded polypeptide chain, can be simply modeled as a mass-spring system from the mechanical viewpoint. Since the conformational flexibility of a protein is dominantly subject to its chemical bond interactions (e.g., covalent bonds, salt bridges, and hydrogen bonds), these constraints can be modeled as linear spring connections between spatially proximal representatives in a variety of coarse-grained ENMs. Coarse-graining approaches enable one to simulate harmonic and anharmonic motions of large macromolecules in a PC, while all-atom based molecular dynamics (MD) simulation has been conventionally performed with an aid of supercomputer. A harmonic analysis of a macroscopic mechanical system, called normal mode analysis, has been adopted to analyze thermal fluctuations of a microscopic biological system around its equilibrium state. Furthermore, a structure-based system optimization, called elastic network interpolation, has been developed to predict nonlinear transition (or folding) pathways between two different functional states of a same macromolecule. The good agreement of simulation and experiment allows the employment of coarse-grained ENMs as a versatile tool for the study of macromolecular dynamics.

• KSBB Journal
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• v.16 no.1
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• pp.1-10
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• 2001

Escherichia coli has been used as an expression work horse for foreign genes. This article summarized recent development in genetic engineering techniques for overproduction of medical proteins and industrial enzymes. Special emphasis was placed upon research activities concerning folding and refolding of inclusion bodies at genetic and fermentation levels. Plasmid and mRNA stabilization, development of strong inducible promoters, modification of translational elements and reduction of rpoteolytic degradation were carried out to elevate an expression level of a target protein. Optimization of culture conditions, improvement of denaturation and renaturation steps and coexpression of molecular chaperones or foldase were accomplished to produce active proteins in soluble form. Fusion protein systems with selective separation and surface display technology were also performed in an effort to make the E. coli expression system more effective and versatile.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.4 no.3
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• pp.681-687
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• 2000

Ideograph is a truly unifies data and procedural dependencies, Ideograph can be used to assist various program optimization, such as common expression elimination, code motion, constant folding etc. In this paper, we design and implementation of the optimized abstract syntax tree using Ideograph. Ideograph has control flow information and data flow information for source program. So we use a Ideograph in order to produce a optimized Ideograph with control flow information and data flow information.

• Journal of Aerospace System Engineering
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• v.12 no.3
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• pp.70-78
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• 2018

This research was carried out to develop an integrated folding wing made from carbon composite materials. Design requirements were reviewed and composite wing sizing was conducted using design optimization with commercial software. Three composite manufacturing processes including hot-press, pultrusion, and autoclave were evaluated and the most suitable processes for the integrated wing fabrication were selected, with consideration given to performance and cost. The determined manufacturing process was verified by two design development tests for selecting the design concept. Stiffness and strength of the composite wing were estimated through structural analyses. The test loads were calculated and static tests about design limit load and design ultimate load were performed using both wings. As a result, the evaluation criterions of the tests were satisfied and structural safety was verified through the series of structural analyses and testing.

• Journal of Microbiology and Biotechnology
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• v.31 no.4
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• pp.570-583
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• 2021

Pyrococcus furiosus α-amylase can hydrolyze α-1,4 linkages in starch and related carbohydrates under hyperthermophilic condition (~ 100℃), showing great potential in a wide range of industrial applications, while its relatively low productivity from heterologous hosts has limited the industrial applications. Bacillus subtilis, a gram-positive bacterium, has been widely used in industrial production for its non-pathogenic and powerful secretory characteristics. This study was conducted to increase production of P. furiosus α-amylase in B. subtilis through three strategies. Initial experiments showed that co-expression of P. furiosus molecular chaperone peptidyl-prolyl cis-trans isomerase through genomic integration mode, using a CRISPR/Cas9 system, increased soluble amylase production. Therefore, considering that native P. furiosus α-amylase is produced within a hyperthermophilic environment and is highly thermostable, heat treatment of intact culture at 90℃ for 15 min was performed, thereby greatly increasing soluble amylase production. After optimization of the culture conditions (nitrogen source, carbon source, metal ion, temperature and pH), experiments in a 3-L fermenter yielded a soluble activity of 3,806.7 U/ml, which was 3.3- and 28.2-fold those of a control without heat treatment (1,155.1 U/ml) and an empty expression vector control (135.1 U/ml), respectively. This represents the highest P. furiosus α-amylase production reported to date and should promote innovation in the starch liquefaction process and related industrial productions. Meanwhile, heat treatment, which may promote folding of aggregated P. furiosus α-amylase into a soluble, active form through the transfer of kinetic energy, may be of general benefit when producing proteins from thermophilic archaea.

• Microbiology and Biotechnology Letters
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• v.36 no.2
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• pp.149-157
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• 2008

Many proteins consist of distinctive domains that can act independently or cooperatively to achieve a unique function. As these domains evolve from a naturally existing repertoire of functional domains, this implies that domain organization is an intrinsic element involved in building the complex structure and function of proteins. Thus, identifying functional domains would appear to be critical to the elucidation of questions related to protein evolution, folding, and the engineering of hybrid proteins for tai- lored applications. However, the simple application of "Lego-like assembly" to the engineering of hybrid proteins is an oversimplification, as many hybrid constructs lack structural stability, usually due to unfavorable domain contacts. Thus, directed evolution, along with computational studies, may help to engineer hybrid proteins with improved physico-chemical properties. Accordingly, this paper introduces several approaches to functional hybrid protein engineering that potentially can be used to create modulators of gene transcription and cell signaling, and novel biosensors to analyze biological functions in vivo.

• Transactions of the Korean Society of Automotive Engineers
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• v.13 no.2
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• pp.108-113
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• 2005

The damageability and repairability of similar platform type vehicles could be very concerned with design optimization. In all the vehicles crash tested, small size passenger vehicles were weakness in aspect of damageability and repairability. The most critical area appears to be repair cost considering that parts cost is the largest portion of total repair cost segments. Besides repair cost, attaching method of front sidemember and subframe are placed special importance for impact energy absorption and damageability and repairability. So in order to improve damageability and repairability of vehicle structure and body component of the monocoque type passenger vehicles, the end of front side member and front back beam should be designed with optimum level and to supply the end of front side member as a partial condition approx 300mm. The effectiveness of design concept on the 40% offset frontal impact characteristics of the passenger vehicle structure is investigated and summarized.