Park, Kyung-Jin;Kroker, Tessa;Gross, Uwe;Zimmermann, Ortrud;Krause, Felix;Haak, Rainer;Ziebolz, Dirk
The korean journal of orthodontics
/
v.49
no.4
/
pp.246-253
/
2019
Objective: To evaluate the effectiveness of three different caries-preventing agents on artificial caries in a Streptococcus mutans-based caries model. Methods: Sixty-five caries-free human molar enamel blocks were treated with a demineralization solution and a remineralization solution. The specimens were assigned to the following groups according to the caries-protective product applied: group A, chlorhexidine varnish; group B, fluoride-releasing chemically cured sealant; group C, fluoride-releasing lightcured sealant; group D, positive control (specimens that were subjected to de- and remineralization cycles without treatment with any caries-protective agents); and group E, negative control (specimens that were not subjected to de- and remineralization cycles). Samples in groups A-D were stored in demineralization solution with S. mutans and thereafter in artificial saliva. This procedure was performed for 30 days. Average fluorescence loss (${\Delta}F$) and surface size of the lesions were measured using quantitative light-induced fluorescence at baseline and on the 7th, 14th, and 30th days. Results: After 30 days, group A demonstrated a significant increase in ΔF and the surface size of the lesions, no significant difference in comparison with the positive control group, and a significant difference in comparison with the negative control group. Group B showed no significant changes in both parameters at any of the measurement points. While group C showed increased ${\Delta}F$ after 14 days, no significant fluorescence change was observed after 30 days. Conclusions: Both fluoride-releasing sealants (chemically or light-cured) show anti-cariogenic effects, but the use of chlorhexidine varnish for the purpose of caries protection needs to be reconsidered.
Kim, Song-Yi;Choi, Sung-Chul;Kim, Kwang-Chul;Choi, Yeong-Chul;Park, Jae-Hong
Journal of the korean academy of Pediatric Dentistry
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v.37
no.3
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pp.288-297
/
2010
The purpose of this study was to evaluate the effect of light cured fluoride-releasing materials on the inhibition of demineralization. In addition, the pattern of fluoride uptake of adjacent tooth structure was analyzed with EPMA. Eighty intact premolars were restored with $Filtek^{TM}$ Z250(control group, composite), Fuji Filling $LC^{TM}$(RMGI), Dyract $AP^{(R)}$ (compomer) and Beautifil II(giomer). Restored teeth were stored in distilled water for 30 days. Then sixty teeth(n=15) were exposed to demineralizing solution(pH 4.3). Demineralized teeth were bisected and polished. The specimens were observed with confocal laser scanning microscope. The depth of outer lesion and the thickness of inhibition zone were measured. Remained twenty teeth(n=5) were bisected for fluoride uptake analysis. The fluoride analysis were taken at enamel-restoration interface and dentin-restoration interface by electron probe micro-analyzer. The results are as follows: 1. The depth of outer lesion of Fuji Filling $LC^{TM}$ Dyract AP, Beautifil II was shallower than that of $Filtek^{TM}$ Z250 at the margin of restoration(p<0.05). 2. The thickness of caries inhibition zone of Fuji Filling $LC^{TM}$, Dyract AP, Beautifil II was greater than that of $Filtek^{TM}$ Z250 at the margin of restoration(p<0.05). 3. Fuji Filling $LC^{TM}$, Dyract AP, Beautifil II groups showed the greater fluoride uptake into enamel and dentine around restoration than $Filtek^{TM}$ Z250 group. 4. In dentin the difference of fluoride concentration were greater than in enamel, and Dyract AP showed the greatest fluoride concentration in dentin.
Journal of the korean academy of Pediatric Dentistry
/
v.42
no.4
/
pp.302-311
/
2015
The purpose of this study was to evaluate the antibacterial properties of a sealant containing S-PRG filler compared to those of two contemporary commercial sealants to determine the inhibition of bacterial growth in broth culture and biofilm formation using the CDC Biofilm Reactor. The BeautiSealant containing S-PRG filler, the fluoride releasing Clinpro$^{TM}$ sealant, which are known to have higher antibacterial effects, and the non-fluoride releasing Concise$^{TM}$ sealant were selected for this study. A Streptococcus mutans culture in BHI broth without sealant served as a negative control in the planktonic growth inhibition test. As a result, bacterial growth was inhibited in all three sealant groups compared to that in the control. The Clinpro$^{TM}$ sealant showed a significantly reduced number of CFUs compared to those of the BeautiSealant and Concise$^{TM}$ sealants. However, no significant difference was detected between the BeautiSealant and Concise$^{TM}$ sealants. The Clinpro$^{TM}$ sealant significantly decreased biofilm formation compared to that by the BeautiSealant and Concise$^{TM}$ sealants. No significant difference was observed between the BeautiSealant and Concise$^{TM}$ sealants. In conclusion, the sealant containing S-PRG filler had a less potent anti-bacterial property and increased biofilm formation capacity compared to those of the fluoride releasing Clinpro$^{TM}$ sealant.
Journal of the korean academy of Pediatric Dentistry
/
v.23
no.2
/
pp.489-501
/
1996
This study was to evaluate shear bond strength of fluoride-releasing sealant and nonfluoride releasing sealant to enamel surface of bovine tooth. 80 extracted bovine teeth were randomly assigned to four groups, and four kinds of sealants including Teethmate-A(Kuraray Co.), Teethmate-F(Kuraray Co.), Helioseal(Vivadent Co.), Helioseal-F(Vivadent Co.) were bonded to exposed enamel surfaces using silicon plate. Shear bond strength was determined in an instron universal testing machine at a crosshead speed of 1mm/min. Then, the fracture surfaces of test specimens were investigated with scanning electron microscope. The obtained results were as follows; 1. The shear bond strength decreased in the following order : Teethmate-A(18.31MPa), Teethmate-F(11.90MPa), Helioseal (11.74 MPa), Helioseal-F(10.64MPa). 2. The shear bond strength of Teethmate-A showed significantly higher than that of Teethmate-F(P<0.05), but Helioseal and Helioseal-F didn't showed statistically different(P<0.05). 3. According to the SEM, Teethmate-A group showed cohesive failure, and Teethmate-A group & Helioseal group showed mixed pattern of cohesive and adhesive failure and Helioseal-F group showed adhesive failure.
Journal of the korean academy of Pediatric Dentistry
/
v.33
no.1
/
pp.25-34
/
2006
Fluoride released from dental restorative materials effectively declines the incidence and activity of dental caries and inhibits tooth demineralization. This study investigated the fluoride release and uptake characteristics of one composite resin $(Z-250^{TM})$ three glass ionomer-based restorative material ($Dyract^{(R)}$ AP, Fuji II $LC^{(R)}$, Fuji IX GP $Fast^{(R)}$) Forty discs(6mm diameter and 1mm height) were prepared for each material. Each disc was immersed in 5ml of distilled water within polyethylene vial and stored at $37^{\circ}C$. The distilled water was changed every 24 hours and the release of fluoride was measured for 31 days. At the end of this period, each specimen was subjected to one of four treatments : (A) no fluoride treatment (control), (B) application of a fluoride dentifrice (500ppm) for three minutes three times; (C) application of the 1.23% acidulated phosphate fluoride(APF) foam for one minute once, (D) the same regimen as (B), plus application of the APF foam for one minute once. Then, all samples were reassessed for an additional 7 days. For all samples, the greatest fluoride release was observed after the first day of the study but diminished with time. On the 7th day of the study, fluoride release level was stabilized. Fuji II $LC^{(R)}$ and Fuji IX GP $Fast^{(R)}$ released higher amount of fluoride than other materials ; however, no statistically significant difference was found from Fuji II $LC^{(R)}$ and Fuji IX GP $Fast^{(R)}$. The amount of fluoride of $Dyract^{(R)}$ AP, Fuji II $LC^{(R)}$ and Fuji IX GP $Fast^{(R)}$ was increased after fluoride treatment, and diminished with time.
Journal of the korean academy of Pediatric Dentistry
/
v.38
no.4
/
pp.327-336
/
2011
The purpose of this study is to investigate the residual fluoride concentration of polymer adhesive tape in oral cavity which is made by spraying NaF on PVA base and to compare with Fluoride varnish(Cavityshiled$^{TM}$). Experimental groups were divided into two according to application methods; Group 1(NaF-PVA tape) and Group 2(Cavityshiled$^{TM}$). Topical fluoride was applied to 20 healthy adults aged from 25 to 30. Fluoride concentration in unstimulated whole saliva was measured by fluoride-sensitive electrode for 72 hours. 1. Until 72 hours after application in every group, significantly higher fluoride concentration was shown in saliva than baseline value(p<0.05). 2. At 2, 3 and 4 hours after application, Group 2 revealed significantly higher fluoride concentration than Group 1(p<0.05). 3. At 24, 48 and 72 hours after application, there was no significance(p>0.05). Although the residual fluoride concentration of saliva and the amount of fluoride of NaF-PVA tape are lower than those of Cavityshield$^{TM}$, NaF-PVA tape is considered to be more effective since it showed almost the same result as Cavityshield$^{TM}$. Therefore, NaF-PVA tape is expected to be a great fluoride application material.
Journal of the korean academy of Pediatric Dentistry
/
v.40
no.2
/
pp.89-97
/
2013
The purpose of this study is to investigate fluoride release in the oral cavity from polymer adhesive tape which is NaF coated PVA. 45 healthy adults were divided into 3 groups by the type of topical fluoride applied: 60seconds taste$^{(R)}$ APF gel (group 1), FluoroDose$^{(R)}$ varnish (group 2) and NaF-PVA (group 3). Topical fluoride was applied to the facial surface of maxillary 12 teeth and unstimulated whole saliva was collected to measure fluoride release after 1, 3, 6, 12, 24, and 48 hours. Fluoride-sensitive electrode was used for measuring the fluoride concentration in the saliva samples. All three groups showed significantly higher value for fluoride concentration than the baseline after 1 and 3 hours (p < 0.05). After 6 hours, group 3 showed significantly higher fluoride concentration than the baseline (p < 0.05) and also showed significantly higher value for fluoride concentration than group 1 and group 2. Between group 1 and group 2, however, there was no significant difference statistically with respect to fluoride concentration value (p > 0.05).
Journal of the korean academy of Pediatric Dentistry
/
v.29
no.4
/
pp.529-538
/
2002
There is no adverse opinion on the anticariogenic effect of fluoride, so glass ionomer restoration which release the fluoride is recommended fer child patient. To study the anticariogenic effect of initial carious lesion of fluoride released from adjacent glass ionomer restoration, the in situ model was constructed. A microhardness test, polarized scope investigation and electron probe microanalysis was done for analyzing the distribution of fluoride which was precipitated from glass ionomer restoration to the enamel caries lesion. Fuji IX, the conventional glass ionomer, was used for experimental group and Z-100, composite resin that fluoride was not contained, was used for control group. On the microhardness test, the remineralization was accelerated by fluoride. And on the polarized investigation, the size of caries lesion was reduced in the oral cavity and that phenomenon was accelerated by fluoride, too. Electron probe microanalysis shows that the remineralization was accelerated by fluoride and the fluoride concentration on subsurface area was increased. It maybe that the subsurface area was critical to anticariogenic effect. In summary of these result, initial caries lesion can be remineralized in the oral cavity and that phenomenon can be accelerated by fluoride. The subsurface area of caries lesion was a major part of defense to cariogenic invasion and to conserve the subsurface area, the surface of lesion body have to conserved.
In this work, we investigate anodization of Pb in ethylene glycol containing small amount of $NH_4F$, demonstrating that ${\beta}-PbF_2$ particles with octahedral morphology can be prepared by adjusting the applied potential and anodizing time. FE-SEM images and XRD measurements of anodic nanostructures as a function of anodizing time clearly show that PbO is first formed on Pb. Subsequently, a local dissolution of PbO leads to formation of skeleton structure of PbO, releasing $Pb^{2+}$ ions in the electrolyte. The lead ions can be precipitated on the walls or intersection of the skeleton walls when the concentration of lead ions is saturated. The method described in this article shows the feasibility of formation of metal fluoride crystal by anodization of metal in a fluoride containing solution.
Journal of the korean academy of Pediatric Dentistry
/
v.34
no.3
/
pp.408-419
/
2007
In the child, it is very important that he/she will have the ability to suppress aesthetic restorative materials of secondary caries. With the representative preventive material against caries, the importance of fluoride is more emphasized. This study examined the differences in fluoride release and re-uptake among some restorative materials, following a treatment of APF gel and fluoride varnish. The surface roughness was observed under scanning electron microscope. Studying this will provide for the research to find effective restorative materials and fluoride type in tooth caries prevention. It is applied from presence at a clinic that restorative materials are resin, flowable resin, compomer and glass ionomer. Fluoride release was measured at 24-hour intervals for 7 days, 3-day intervals from 8th to 38th day using an ion-selective electrode and analyzer. Then, the materials were treated with the fluoride gel and fluoride varnish respectively, fluoride release was measured and specimens were evaluated under scanning electron microscope for 4 weeks. It was concluded that 1. Fluoride was released for 38 days from restorative materials under 1 ppm in case of flowable resin, 1-2 ppm in compomer and 2-8 ppm in glass ionomer, a few of fluoride was released after 45 days 2. Fluoride has more releasing after application of APF gel than fluoride varnish. Fluoride re-uptake was observed under 0.6-0.2 ppm in fluoride varnish and 0.6-2.6 ppm in APF gel after starting the procedure one day(p<0.05). For the remaining 4 weeks, they demonstrated a similar release. 3. Specimens were evaluated under scanning electron microscope. Applied fluoride in the experimental group surface was rougher than the control group that did not receive fluoride application. Fluoride varnish group had a smoother surface than both the APF gel group and the varnish APF gel group that received a fluoride application.
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