• 제목/요약/키워드: Fluorescent intensity

검색결과 281건 처리시간 0.024초

Low-Pressure Mercury-Free Fluorescent Lamp for General Lighting

  • Jinno, Masafumi;Lee, Jong-Chan;Motomural, Hideki;Aono, Masaharu;Park, Dae-Hee
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2004년도 추계학술대회 논문집 전기물성,응용부문
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    • pp.11-14
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    • 2004
  • In this paper we report on development of our original mercury-less fluorescent lamps for general lighting. These new-type lamps have multi-pairs of electrodes to maintain multi-discharge paths and to keep positive column diffused state. The omission characteristics oft the lamps were investigated. These discharge lamps were operated by pulsed discharge. By using multi-pairs of electrodes for xenon pulsed discharge fluorescent lamps, higher intensity of luminance was obtained than the conventional type of lamps using single pair of electrodes. Recently this type of lamp achieved over $9000\;cd/m^2$ and 50 lm/W.

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Fluorescence Intensity Changes for Anthrylazacrown Ethers by Paramagnetic Metal Cations

  • 장정호;김해중;박중희;신영국;정용석
    • Bulletin of the Korean Chemical Society
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    • 제20권7호
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    • pp.796-800
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    • 1999
  • Three anthrylazacrown ethers in which the anthracene fluorophore π system is separated from the electron donor atoms by one methylene group were synthesized, and their photophysical study was accomplished. These fluorescent compounds showed a maximum fluorescence intensity at pH=5 in aqueous solutions and a decrease in fluorescence intensity upon binding of paramagnetic metal cations (Mn 2+ (d 5 ), Co 2+ (d 7 ), Cu 2+ (d 9 )). The decrease in fluorescence intensity may be attributed to the paramagnetic effect of metal cations to deactivate the excited state by the nonradiative quenching process. The benzylic nitrogen was found to play an important role in changing fluorescence intensity. From the observed linear Stern-Volmer plot and the fluorescence lifetime independence of the presence of metal ions, it was inferred that the chelation enhanced fluorescence quenching (CHEQ) mechanism in the system is a ground state static quenching process. Enhanced fluorescence was also observed when an excess Na + ion was added to the quenched aqueous solution, and it was attributed to cation displacement of a complexed fluorescence quencher.

광 픽업 방식 형광스캐너를 이용한 미소유체 특성 분석 (Characterization of Microfluidic Channels using DVD Pick-up Fluorescent Scanner)

  • 임빛;김재현;이승엽;박정열
    • 대한기계학회논문집A
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    • 제32권12호
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    • pp.1102-1106
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    • 2008
  • Microfluidics deals with the behavior, precise control and manipulation of fluids at a micro scale. It has become increasingly prevalent in various applications such as biomedical applications (diagnostics, therapeutics, and cell/tissue engineering), inkjet head, and fuel cells etc. The issue of inspection and characterization of microfluidics has emerged as a major consideration in design, fabrication, and detection of microfluidic devices. In this paper, we characterize a diffusion based mixing in Y-microchannel using a fluorescent optical scanner based on a DVD pick-up module, which is widely used in optical storages. Using fluorescent dye, we measure the fluorescent intensity that represents the mixing patterns in Y-microchannel. We also compare these experimental results with computational fluid dynamics (CFD) simulation ones. It is shown that the proposed optical scanner can be used as an alternative measurement system with high performance and cost-effectiveness, compared to conventional optical tools such as epifluorescent microscopes using high resolution CCD camera and confocal microscopes with photomultiplier (PMT) detectors.

고속액체크로마토그라피에 의한 프로스타글란딘류의 고감도 형광 분석법 (A Sensitive Fluorescent Detection Method for Prostaglandins by High Performance Liquid Chromatography)

  • 이용문;문동철
    • 약학회지
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    • 제36권5호
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    • pp.506-511
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    • 1992
  • The Prostaglandins were derivatized rapidly with monodansyl cadaverine as a fluorophore in mild conditions. The carboxylic moiety of prostaglandins was activated with diethyl phosphorocyanidate and successively coupled with fluorophore in dimethylformamide at room temperature. The labeling yield was reached about 95% at 15 min using arachidic acid $(C_{20:0})$ as a test sample. This derivative showed constant fluorescent intensity at $4^{\circ}C$ for 180 days. The derivatives of prostaglandins were shown high solvent selectivity with tetrahydrofuran in reversed-phase column. therefore, these derivatives could be successfully separated on YMC pack A-212(S-5 120A C8) column in tetrahydrofuran-based eluents. The detection limits of these derivatives was ca. 500 fmol and determination limits was ca. 5 pmol as injected amount in fluorescent detection $({\lambda}ex.\;340\;nm,\;{\lambda}em.\;520\;nm)$. In this method, the ranges of recovery and coefficient of variation were $93.6{\sim}102.7%$ and $4.3{\sim}5.8%$, respectively.

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마이크로플레이트 기반 생물반응기 시스템 (MABOOMSTM)을 이용한 대장균 배양공정에서 용존산소, pH 및 세포농도의 온라인 모니터링 연구 (Study on Online Monitoring of Dissolved Oxygen, pH and Cell Concentration in E. coli Cultivation Processes Using MABOOMSTM)

  • 손옥재;이종일
    • KSBB Journal
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    • 제28권1호
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    • pp.24-30
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    • 2013
  • Dissolved oxygen, pH and cell concentration have been online monitored in cultivation processes with Escherichia coli by using a $MABOOMS^{TM}$ (microplate-based bioreactor with optical online monitoring systems). Fluorescent sensing membranes containing Ru ${(dpp)_3}^{2+}$ or HPTS were prepared with GA sol-gel matrix and coated into a well of a 24-well microplate. Fluorescence intensity was measured and correlated to the dissolved oxygen or pH. Cell concentrations were also online monitored by measuring optical reflectance at 650 nm. A well of a 24-well microplate could also be divided into 4 parts, each of which was coated with fluorescent sensing membranes for the detection of dissolved oxygen or pH. The 24-well microplate coated with fluorescent sensing membranes or a 4-divided sensing membrane. was used to online monitor the dissolved oxygen, pH and cell concentration during E. coli cultivations. The online monitoring results showed the characteristics of cell growth in cultivation processes very well.

Development of a Fluorescent Sensor Based on Resazurin and Hydrotalcite for the Determination of Ethanol in Alcoholic Beverages

  • Hong Dinh Duong;Juyeon Kim;Jong Il Rhee
    • 센서학회지
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    • 제33권2호
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    • pp.70-77
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    • 2024
  • In this study, a fluorescent ethanol sensor is developed to determine the ethanol concentration in the liquid phase. The sensor is developed using a complex of resazurin (RA)/resorufin (RO) and a hydrotalcite (HT) catalyst in a sol-gel matrix of methyltrimethoxysilane (MTMS) to produce a fluorescent ethanol-sensing membrane (RA/RO*HT membrane). The operation mechanism of the RA/RO*HT membrane is based on (i) the oxidation of ethanol to acetaldehyde and (ii) the reduction of RA to RO, through electron flows followed by EtOH ↔ HT ↔ RA/RO ↔ EtOH interactions. These possible redox reactions can lead to an increased fluorescence intensity of the RA/RO*HT membrane as the ethanol concentration increases. The RA/RO*HT membrane shows a linear detection range of 1-20 vol.% EtOH with limit of detection (LOD) of 0.178%. Additionally, the RA/RO*HT membrane has high sensitivity and accuracy for determining the alcohol content in several Korean alcoholic beverages.

복합 광원을 갖는 형광 내시경 개발 (Development of the Fluorescence Endoscope System with Dual Light Source Apparatus)

  • 배수진;강욱
    • 전기학회논문지
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    • 제56권1호
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    • pp.222-226
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    • 2007
  • We suggest the fluorescence endoscope system that has light source apparatus providing selectable white or excitation light. White light source generates normal color images and is easily switched over to excitation light with the wide spectrum range from 380 nm to 580 nm. 5-ALA is deposited selectively in the abnormal tissue like cancer and causes fluorescence in the red spectrum range when excited by blue spectrum range. In addition, the others of excitation light make the color background image by reflected light to allow accurate orientation and visualization of the abnormal tissue and around. According to clinical studies, the fluorescence intensity contrast that defines the fluorescence intensity of lesion over the fluorescence intensity of around has more than 2 in tumour. Proposed system is useful and objective way in early diagnosis. Furthermore, it can be used in the biopsy for tumour classification at the highest fluorescence intensity point.

간접 반사형 LED 조명기구의 반사판 형태에 따른 배광형태 및 조명환경 변화 분석 (Analysis of the Change of Intensity Distribution and Luminous Environment by Reflector Shape in Indirect Reflected LED Luminaires)

  • 김유신;최안섭
    • 조명전기설비학회논문지
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    • 제25권1호
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    • pp.9-17
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    • 2011
  • Development of high efficiency and eco-friendly LED source has been progressed continuously. Also, by nation's regulations, public institutions have changed existing luminaires to LED luminaires. However, there are several differences between existing luminaires and the intensity distribution of general planar LED luminaires. In case of changing existing luminaires to LED luminaires, by such differences, there are inefficiencies both quality aspect and energy aspect in luminous environment. Therefore, this study aims to conduct a study on the changes of intensity distribution and luminous environment by reflector shapes in indirect reflected LED luminaires with software for optical design of a luminaire (Photopia 2.0) and lighting design of a space (ReluxPro). This study shows the changes of intensity distribution and luminous environment of indirect reflected LED luminaires and indirect fluorescent lamp luminaires by reflector shapes.

The Fluorescent Effects on the N-Substituents of Polyarylenevinylenes Having 1,2-Diphenylmaleimide Moieties

  • Lee, Jun-Kyu;Yang, Nam-Choul;Park, Ho-Wook;Suh, Dong-Hack
    • Macromolecular Research
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    • 제11권2호
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    • pp.92-97
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    • 2003
  • Model compounds and polymers having N-substituted 1,2-diphenylmaleimide moieties were synthesized and characterized by NMR, IR, UV, and fluorescence spectroscopy. The fluorescence intensity could be controlled by N-substituents of model compounds and polymers. As the structure of an N-substituent of them was bulkier, or the electron density of an N-substituent was denser, the photoluminescence intensity was increased. All the compounds showed greenish yellow photoluminescence with the maximum intensity between 510 and 537 nm. From quantum efficiency data of the model compounds and the polymers, the fluorescence intensity of the polymer 11 was higher than that of the model compound 4.

Effects of Light Pulse Intensity and Quencher Concentration on the Time-Dependent Fluorescence Quenching Kinetics

  • Yang Mino;Lee Sangyoub;Shin, Kook Joe;Choo Kwang Yul;Lee Duckhwan
    • Bulletin of the Korean Chemical Society
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    • 제13권3호
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    • pp.325-331
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    • 1992
  • By using the general theoretical framework proposed recently for treating the fluorescence quenching kinetics, we investigate the effect of light pulse intensity on the decay of fluorescence which follows excitation of fluorophors by the light pulse of very short but finite duration. It is seen that conventional theory breaks down when the exciting light pulse has a pulse width comparable to the fluorescent lifetime and its intensity is very high. We also find that even when the light intensity is not too high, conventional theory may fail in either of the following cases: (i) when the quencher concentration is high, (ii) when there is an attractive potential of mean force between the fluorophor and quencher, or (iii) when the energy transfer from the fluorophor to the quencher may also occur at a distance, e.g., via dipole-dipole interaction. The validity of the predictions of the present theory may thus be tested by fluorescence quenching experiments performed under such situations.