• 통합자연과학논문집
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• 제3권4호
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• pp.215-218
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• 2010

cis,cis-1,2,3,4-Tetraphenylbutadiene has been synthesized and its optical properties are investigated by using UV-Vis absorption and fluorescence spectroscopy. Thin films of tetraphenylbutadiene prepared from thin layer chromatography(TLC) displays strong luminescence and used for the detection of vapor of organic halide. Tetraphenylbutadiene shows dramatic quenching photoluminescence under exposure of chloroform vapor.

• 대한의생명과학회지
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• 제22권4호
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• pp.160-166
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• 2016

To provide a basis for a homogeneous fluorescence resonance energy transfer (FRET) immunoassay for celiac disease, we carried out a FRET experiment using guinea pig tissue transglutaminase (tTG) and antibodies to tTG (anti-tTG) purified from rat serum. Fluorescein was utilized as the probe, and a nonfluorescent dye, QSY 7 served as the quencher. We labeled anti-tTG and tTG with fluorescein isothiocyanate and QSY 7 succinimidyl ester, respectively. Fluorescein-labeled anti-tTG was the donor, and QSY 7-labeled tTG was the acceptor of the FRET experiment. When we titrated fluorescein-labeled anti-tTG with QSY 7-labeled tTG, we observed a large decrease in the steady-state fluorescence intensity, which was due to strong FRET from fluorescein-labeled anti-tTG to QSY 7-labeled tTG. Using time-resolved fluorescence spectroscopy, we could also observe a decrease in the fluorescence lifetime, which confirms the steady-state data. We expect that these results might be useful in the development of a novel fluorescence immunoassay for an easy screening and follow-up of celiac patients.

• 한국응용과학기술학회지
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• 제23권4호
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• pp.340-346
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• 2006

The influences of fluorescence, scattering, and flocculation in turbid material by light scattering were interpreted for the scattered fluorescence intensity and wavelength, it has been studied the molecular properties by the spectroscopy of laser induced fluorescence (LIF). The effects of optical properties in scattering media have been found by the optical $parameters({\mu}_s,\;{\mu}_a,\;{\mu}_t)$. Flocculation is an important step in many solid-liquid separation processes and is widely used. When two particles approach each other, interactions of several colloid particles can come into play which may have major effect on the flocculation and LIF process, The value of scattering coefficient ${\mu}_s$ is large by means of the increasing particles of scatterer it has been found that the slope decays exponentially as a function of distance from laser source to detector. It may also aid in designing the best model for oil chemistry, biopharmaceutical products, laser medicine and application of medical engineering on LIF and coagulation in particle transport mode.

• Bulletin of the Korean Chemical Society
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• 제32권2호
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• pp.583-589
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• 2011

Fluorescence correlation spectroscopy (FCS) is a sophisticated and an accurate analytical technique used to study the diffusion of molecules in a solution at the single-molecule level. FCS is strongly affected by many factors such as the stability of the excitation power, photochemical processes, mismatch between the refractive indices, and variations in the cover glass thickness. We have studied FCS near the surface of a cover glass by using rhodamine 123 as a fluorescent probe and have observed that the surface has a strong influence on the measurements. The temporal autocorrelation of FCS decays with two characteristic times when the confocal detection volume is positioned near the surface of the cover glass. As the position of the detection volume is moved away from the surface, the FCS autocorrelation becomes one-component decaying; the characteristic time of the decay is the same as the faster-decaying component in the FCS autocorrelation near the surface. This observation suggests that the faster component can be attributed to the free diffusion of the probe molecules in the solution, while the slow component has its origin from the interaction between the probe molecules and the surface. We have characterized the surface contribution to the FCS measurements near the surface by changing the position of the detection volume relative to the surface. The influence of the surface on the diffusion of the probe molecules was monitored by changing the chemical properties of the surface. The surface contribution to the temporal autocorrelation of the FCS strongly depends on the chemical nature of the surface. The hydrophobicity of the surface is a major factor determining the surface influence on the free diffusion of the probe molecules near the surface.

• 한국식품과학회지
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• 제48권5호
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• pp.418-423
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• 2016

근적외선분광분석기와 에너지 분산형 X선 형광분석기를 이용한 분석방법을 개발하여 각각 97.5, 98.0%의 높은 정확도의 판별식을 확립하였고, 시중 유통 시료를 분석하여 검증한 결과 각각 96.3, 95.0%의 판별 정확도를 확인하였다. 이상의 연구 결과를 통하여 근적외선분광분석기와 에너지 분산형 X선 형광분석기를 이용하여 청국장 원산지 판별이 가능함을 확인하였고 이는 유기성분 함량에 따른 근적외선 흡광도와 무기성분 함량에 따른 X선 형광에너지 강도가 국내산과 수입산 간에 차이가 있기 때문으로 사료된다.

• 대한의용생체공학회:의공학회지
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• 제33권3호
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• pp.114-127
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• 2012

Recent technical advancement allows noninvasive measurement of blood glucose. In this literature, we reviewed various noninvasive techniques for measuring glucose concentration. Optical or electrical methods have been investigated. Optical techniques include near-infrared spectroscopy, Raman spectroscopy, optical coherence technique, polarization, fluorescence, occlusion spectroscopy, and photoacoustic spectroscopy. Electrical methods include reverse iontophoresis, impedance spectroscopy, and electromagnetic sensing. Ultrasound, detection from breath, or fluid harvesting technique can be used to measure blood glucose level. Combination of various methods is also promising. Although there are many interesting and promising technologies and devices, there need further researches until a commercially available non-invasive glucometer is popular.

• Journal of Biosystems Engineering
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• 제42권3호
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• pp.170-189
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• 2017

Purpose: This paper presents Raman chemical imaging technology for inspecting food and agricultural products. Methods The paper puts emphasis on introducing and demonstrating Raman imaging techniques for practical uses in food analysis. Results & Conclusions: The main topics include Raman scattering principles, Raman spectroscopy measurement techniques (e.g., backscattering Raman spectroscopy, transmission Raman spectroscopy, and spatially offset Raman spectroscopy), Raman image acquisition methods (i.e., point-scan, line-scan, and area-scan methods), Raman imaging instruments (e.g., excitation sources, wavelength separation devices, detectors, imaging systems, and calibration methods), and Raman image processing and analysis techniques (e.g., fluorescence correction, mixture analysis, target identification, spatial mapping, and quantitative analysis). Raman chemical imaging applications for food safety and quality evaluation are also reviewed.

• 폴리머
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• 제25권2호
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• pp.199-207
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• 2001

본 연구에서는 경화촉진제로서 N,N-dimethyl benzyl amine (BDMA) 또는 1-cyanoethyl-2-ethyl-4-methyl imidazole (2E4MZ-CN)을 포함하고 있는 산무수물계 경화제에 의한 diglycidyl ether of bisphenol-A (DGEBA)의 경화거동을 형광분석기를 사용하여 광물리적 특성 변화의 관점에서 해석하였다. 이를 위해 1,3-bis-(1-pyrene)propane (BPP) probe를 에폭시수지 내에 균일하게 도입시켰다. 주위 환경 겔화에 의한 분자의 공간구조 변화에 민감한 BPP probe는 분자내 여기체 형광을 잘 형성하였으며 에폭시수지의 경화반응에 따른 미세점도 변화 또는 분자의 움직임에 민감하였다. 에폭시수지의 경화거동은 경화시간, 경화온도 및 경화촉진제의 종류에 따른 단량체 형광세기 ($I_{M}$ ), 여기체 형광세기 ($I_{E}$ ) 그리고 $I_{M}$ /$I_{E}$ 값의 변화로부터 설명되었다. 그 결과는 이전의 DSC 또는 torsion pendulum을 이용하여 에폭시-산무수물계에 대하여 얻어진 경화거동 결과와 일치하였다. 또한, 형광분석 방법은 다른 분석 방법에서 해석이 어려운 저온영역에서의 열경화성 수지의 경화거동에 대한 정보를 제시하였다.

• 접착 및 계면
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• 제1권1호
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• pp.47-55
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• 2000

In this article, the characterization of the interface of hybrid composites was discussed. Interfacial interaction in organic/inorganic hybrid composites, especially silica-containing hybrids can be characterized by fluorescence spectroscopy, small angle X-ray scattering (SAXS), scanning electron microscopy (SEM), atomic force microscopy (AFM), and $^{29}Si$ NMR spectroscopy measurements.

• BMB Reports
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• 제32권6호
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• pp.521-528
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• 1999

The folding of recombinant hepatitis B virus X-protein (rHBx) solubilized from Escherichia coli inclusion bodies was investigated. By sequential dialysis of urea, rHBx was folded into its native structure, which was demonstrated by the efficacy of its transcriptional activation of the adenovirus major late promoter (MLP), fluorescence spectroscopy, and circular dichroism (CD) analysis. The decrease in CD values at 220 nm and a corresponding blue shift of the intrinsic fluorescence emission confirmed the ability of rHBx to refold in lower concentrations of urea, yielding the active protein. Equilibrium and kinetic studies of the refolding of rHBx were carried out by tryptophan fluorescence measurements. From the biphasic nature of the fluorescence curves, the existence of stable intermediate states in the renaturation process was inferred. Reverse phase-high performance liquid chromatography (RP-HPLC) analysis further demonstrated the existence of these intermediates and their apparent compactness.