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http://dx.doi.org/10.15616/BSL.2016.22.4.160

A FRET Assay for Celiac Disease  

Lee, Sae A (Department of Oral Biochemistry and Molecular Biology, School of Dentistry, Pusan National University)
Cho, Chul Min (Department of Oral Biochemistry and Molecular Biology, School of Dentistry, Pusan National University)
Jang, Il Ho (Department of Oral Biochemistry and Molecular Biology, School of Dentistry, Pusan National University)
Kang, Jung Sook (Department of Oral Biochemistry and Molecular Biology, School of Dentistry, Pusan National University)
Publication Information
Biomedical Science Letters / v.22, no.4, 2016 , pp. 160-166 More about this Journal
Abstract
To provide a basis for a homogeneous fluorescence resonance energy transfer (FRET) immunoassay for celiac disease, we carried out a FRET experiment using guinea pig tissue transglutaminase (tTG) and antibodies to tTG (anti-tTG) purified from rat serum. Fluorescein was utilized as the probe, and a nonfluorescent dye, QSY 7 served as the quencher. We labeled anti-tTG and tTG with fluorescein isothiocyanate and QSY 7 succinimidyl ester, respectively. Fluorescein-labeled anti-tTG was the donor, and QSY 7-labeled tTG was the acceptor of the FRET experiment. When we titrated fluorescein-labeled anti-tTG with QSY 7-labeled tTG, we observed a large decrease in the steady-state fluorescence intensity, which was due to strong FRET from fluorescein-labeled anti-tTG to QSY 7-labeled tTG. Using time-resolved fluorescence spectroscopy, we could also observe a decrease in the fluorescence lifetime, which confirms the steady-state data. We expect that these results might be useful in the development of a novel fluorescence immunoassay for an easy screening and follow-up of celiac patients.
Keywords
Fluorescence resonance energy transfer; Tissue transglutaminase; Celiac disease; Anti-tTG antibodies;
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1 Leffler DA, Edwards George JB, Dennis M, Cook EF, Schuppan D, Kelly CP. A prospective comparative study of five measures of gluten-free diet adherence in adults with celiac disease. Aliment Pharmacol Ther. 2007. 26: 1227-1235.   DOI
2 Lenhardt A, Plebani A, Marchetti F, Gerarduzzi T, Not T, Meini A, Villanacci V, Martelossi S, Ventura A. Role of human-tissue transglutaminase IgG and anti-gliadin IgG antibodies in the diagnosis of coeliac disease in patients with selective immunoglobulin A deficiency. Dig Liver Dis. 2004. 36: 730-734.   DOI
3 Li M, Yu L, Tiberti C, Bonamico M, Taki I, Miao D, Murray JA, Rewers MJ, Hoffenberg EJ, Agardh D, Mueller P, Stern M, Bonifacio E, Liu E. A report on the international transglutaminase autoantibody workshop for celiac disease. Am J Gastroenterol. 2009. 104: 154-163.   DOI
4 Rittmeyer C, Rhoads JM. IgA deficiency causes false-negative endomysial antibody results in celiac disease. J Pediatr Gastroenterol Nutr. 1996. 23: 504-506.   DOI
5 Sollid LM. Molecular basis of celiac disease. Annu Rev Immunol. 2000. 18: 53-81.   DOI
6 Sollid LM, Jabri B. Triggers and drivers of autoimmunity: lessons from coeliac disease. Nat Rev Immunol. 2013. 13: 294-302.   DOI
7 Suh-Lailam BB, Davis KW, Tebo AE. Immunoassays for the detection of IgA antibodies to tissue transglutaminase: significance of multiples of the upper limit of normal and inter-assay correlations. Clin Chem Lab Med. 2016. 54: 257-264.
8 Basso D, Guariso G, Fasolo M, Pittoni M, Schiavon S, Fogar P, Greco E, Navaglia F, Zambon CF, Plebani M. A new indirect chemiluminescent immunoassay to measure anti-tissue transglutaminase antibodies. J Pediatr Gastroenterol. 2006. 43: 613-618.   DOI
9 Vetrano S, Zampaletta U, Anania MC, Tola MD, Sabbatella L, Passarelli F, Maffia C, Sanjust MG, Lettieri F, De Pita O, Picarelli A. Detection of anti-endomysial and anti-tissue transglutaminase autoantibodies in media following culture of oral biopsies from patients with untreated coeliac disease. Dig Liver Dis. 2007. 39: 911-916.   DOI
10 AGA Institute. AGA Institute medical position statement on the diagnosis and management of celiac disease. Gastroenterology. 2006. 131: 1977-1980.   DOI
11 Basso D, Guariso G, Fogar P, Meneghel A, Zambon CF, Naviglia F, Greco E, Schiavon S, Rugge M, Plebani M. Antibodies against synthetic deamidated gliadin peptides for celiac disease diagnosis and follow-up in children. Clin Chem. 2009. 55: 150-157.
12 Bonamico M, Nenna R, Luparia RPL, Perricone C, Montuori M, Lucantoni F, Castronovo A, Mura S, Turchetti A, Strappini P, Tiberti C. Radioimmunological detection of anti-transglutaminase autoantibodies in human saliva: a useful test to monitor celiac disease follow-up. Aliment Pharmacol Ther. 2008. 28: 364-370.   DOI
13 Collin P, Maki M, Keyrilainen O, Hallstrom O, Reunala T, Pastenack A. Selective IgA deficiency and coeliac disease. Scand J Gastroenterol. 1992. 27: 367-371.   DOI
14 Bonamico M, Tiberti C, Picarelli A, Mariani P, Rossi D, Cipolletta E, Greco M, Tola MD, Sabbatella L, Carabba B, Magliocca FM, Strisciuglio P, Di Mario U. Radioimmunoassay to detect antitransglutaminase autoantibodies is the most sensitive and specific screening method for celiac disease. Am J Gastroenterol. 2001. 96: 1536-1540.   DOI
15 Catassi C, Fasano A. Celiac disease. Curr Opin Gastroenterol. 2008. 24: 687-691.   DOI
16 Chorzelski TP, Beutner EH, Sulej J, Tchorzewska H, Jablonska S, Kumar V, Kapuscinska A. IgA anti-endomysium antibody. A new immunological marker of dermatitis herpetiformis and coeliac disease. Br J Dermatol. 1984. 111: 395-402.   DOI
17 Dieterich W, Ehnis T, Bauer M, Donner P, Volta U, Riecken EO, Schuppan D. Indentification of tissue trnasglutaminase as the autoantigen of celiac disease. Nat Med. 1997. 3: 798-801.
18 Compilato D, Campisi G, Pastore L, Carroccio A. The production of the oral mucosa of antiendomysial and anti-tissuetransglutaminase antibodies in patients with celiac disease: a review. The Scientific World Journal. 2010. 10: 2385-2394.   DOI
19 Condo R, Costacurta M, Docimo R. The anti-transglutaminase auto-antibodies in children's saliva with a suspect coeliac disease: clinical study. Oral Implantol. 2013. 6: 48-54.
20 D'Auria S, Apicella E, Staiano M, Giovanni S, Riggiero G, Rossi M, Sarkar P, Luchowski R, Gryczinski I, Gryczinski Z. Engineering resonance energy transfer for advanced immunoassays: the case of celiac disease. Anal Biochem. 2012. 425: 13-17.   DOI
21 Greenbaum CJ, Palmer JP, Kuglin B, Kolb H. Insulin autoantibodies measured by radioimmunoassay methodology are more related to insulin-dependent diabetes mellitus than those measured by enzyme-linked immunoassorbent assay: results of the fourth international workshop in the standardization of insulin autoantibody measurement. J Clin Endocrinol Metab. 1992. 74: 1040-1044.
22 Green PH, Jabri B. Coeliac disease. Lancet. 2003. 362: 383-391.   DOI
23 Hill ID. What are the sensitivity and specificity of serologic tests for celiac disease? Do sensitivity and specificity vary in different population?. Gastroenterology. 2005. 128: S25-32.   DOI
24 Hill ID, Dirks MH, Liptak GS, Colletti RB, Fasano A, Guandalini S, Hoffenberg EJ, Horvath K, Murray JA, Pivor M, Seidman EG. Guideline for the diagnosis and treatment of celiac disease in children: recommendations of the North American Society for Pediatric Gastroenterology, Hepatology and Nutrition. J Pediatr Gastroenterol Nutr. 2005. 40: 1-19.   DOI
25 Lakowicz JR, Laczko G, Cherek H, Gratton E, Limkeman M. Analysis of fluorescence decay kinetics from variablefrequency phase-shift and modulation data. Biophys J. 1984. 46: 463-477.   DOI
26 Husby S, Koletzko S, Korponay-Szabo IR, Mearin ML, Phillips A, Shamir R, Troncone R, Giersiepen K, Branski D, Catassi C, Lelgeman M, Maki M, Ribes-Koninckx C, Ventura A, Zimmer KP. European Society for Pediatric Gastroenterology, Hepatology, and Nutrition guidelines for the diagnosis of coeliac disease. J Pediatr Gastroenterol Nutr. 2012. 54: 136-160.   DOI
27 Lakowicz JR. Principles of Fluorescence Spectroscopy. 2006. 443-451.