• 대한전기학회:학술대회논문집
• /
• 대한전기학회 2003년도 추계학술대회 논문집 전기물성,응용부문
• /
• pp.89-91
• /
• 2003

The determination of DNA hybridization reaction can apply the molecular biology research, clinic diagnostics, bioengineering, environment monitoring, food science and other application area. So, the improvement of DNA detection system is very important for the determination of this hybridization reaction. In this study, we report the characterization of the probe and target oligonucleotide hybridization reaction using the evanescent field microscopy. First, we have fabricated DNA chip microarray. The particles which were immobilized oligonucleotides were arranged by the random fluidic self-assembly on the pattern chips, using hydrophobic interaction. Second, we have detected DNA hybridization reaction using evanescent field microscopy. The 5'-biotinylated probe oligonucleotides were immobilized on the surface of DNA chip microarray and the hybridization reaction with the Rhodamine conjugated target oligonucleotide was excited fluorescence generated on the evanescent field microscopy. In the foundation of this result, we could be employed as the basis of a probe olidonucleotide, capable of detecting the target oligonucleotide and monitoring it in a large analyte concentration range and various mismatching condition.

• Interdisciplinary Bio Central
• /
• 제4권2호
• /
• pp.5.1-5.9
• /
• 2012

Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Western-blotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose "Estern staining", as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming "immuno-staining" in many versatile applications.

• KIEE International Transactions on Electrophysics and Applications
• /
• 제11C권3호
• /
• pp.85-90
• /
• 2001

The determination of DNA hybridization reaction can apply the molecular biology research, clinic diagnostics, bioengineering, environment monitoring, food science and other application area. So, the improvement of DNA detection system is very important for the determination of this hybridization reaction. In this study, we report the characterization of the probe and target oligonucleotide hybridization reaction using the evanescent field microscopy. First, we have fabricated DNA chip microarray. The particles which were immobilized oligonucleotides were arranged by the random fluidic self-assembly on the pattern chips, using hydrophobic interaction. Second, we have detected DNA hybridization reaction using evanescent field microscopy. The 5'-biotinylated probe oligonucleotides were immobilized on the surface of DNA chip microarray and the hybridization reaction with the Rhodamine conjugated target oligonucleotide was excited fluorescence generated on the evanescent field microscopy. In the foundation of this result, we could be employed as the basis of a probe olidonucleotide, capable of detecting the target oligonucleotide and monitoring it in a large analyte concentration range and various mismatching condition.

• Journal of Microbiology and Biotechnology
• /
• 제22권2호
• /
• pp.248-255
• /
• 2012

In order to develop a protocol to quantify cyanobacteria and Microcystis simultaneously, the primers and probe were designed from the conserved regions of 16S rRNA gene sequences of cyanobacteria and Microcystis, respectively. Probe match analysis of the Ribosomal Database Project showed that the primers matched with over 97% of cyanobacterial 16S rRNA genes, indicating these can be used to amplify cyanobacteria specifically. The TaqMan probe, which is located between two primers, matched with 98.2% of sequences in genus GpXI, in which most Microcystis strains are included. The numbers of cyanobacterial genes were estimated with the emission of SYBR Green from the amplicons with two primers, whereas those of Microcystis spp. were measured from the fluorescence of CAL Fluor Gold 540 emitted by exonuclease activity of Taq DNA polymerase in amplification. It is expected that this method enhances the accuracy and reduces the time to count cyanobacteria and potential toxigenic Microcystis spp. in aquatic environmental samples.

• Bulletin of the Korean Chemical Society
• /
• 제28권10호
• /
• pp.1818-1820
• /
• 2007

• Bulletin of the Korean Chemical Society
• /
• 제31권1호
• /
• pp.230-233
• /
• 2010

• Applied Biological Chemistry
• /
• 제25권4호
• /
• pp.224-231
• /
• 1982

liposome에 결합되어 있는 ${\alpha}-Tocopherol$의 형광을 이용하여 liposome의 상전이온도를 측정할 수 있었다. 이는 ${\alpha}-Tocopherol$의 vitamin E로서의 중요성 이외에 형광성 프로브로서의 새로운 가치의 발견이다. 형광성 프로브로서 그 응용의 확대를 위한 필수적인 기초자료의 수집을 위해, 분자분광학적성질을 조사하였다. 용액중의 ${\alpha}-Tocopherol$은 monomer와 dimer의 두가지 형태로 존재하며, 단파장흡수대$(291{\sim}294nm)$의 monomer는 형광성인데 반하여 dimer는 298nm 부근을 흡수하는 비형광성이고 분자간수소결합에 의하여 형성되었음이 밝혀졌다. liposome에 결합된 ${\alpha}-Tocopherol$은 각종 유기용매 중에서의 ${\alpha}-Tocopherol$과는 판이한 형광성질을 갖고 있었고 chromanolate이온형태의 화학종으로부터 생긴 것으로 해석되었다.

• 한국가금학회:학술대회논문집
• /
• 한국가금학회 2004년도 제21차 정기총회 및 학술발표회
• /
• pp.13-15
• /
• 2004

Telomere는 진핵세포염색체 말단부에 TTAGGG 반복 염기서열을 가지는 DNA-protein 복합체로 세포 분열시마다 짧아지며, 발생 및 노화와 밀접한 관련이 있는 것으로 알려져 있다. 본 연구는 닭에 있어 telomere의 양적 분포양상을 구명함으로써 이를 이용한 개체의 생명표지 (bio-marker)의 가능성을 탐색코자 하였다. 본 분석에 이용된 계종으로는 한국재래계와 단관 백색화이트 레그혼종을 대상으로 하였고, 주령간, 품종간 및 성간 백혈구내 telomere 함량을 비교 분석하였으며, 또한 분석개체들의 생산능력과 이들의 telomere 함유율 간의 상관관계를 조사하였다. Telomere의 양적 분석은 chicken telomeric DNA probe를 이용한 양적 형광접합보인법(Quantitative fluorescence in situ hybridization : Q-FISH)을 이용하였다. Telomere 양적 분석결과. 주령이 증가함에 따라 telomere 함량이 유의적으로 감소됨을 확인하였고, 품종간 및 성간에도 유의적인 차이가 나타났다. 또한 생산능력과 각 개체의 telomere 함량간의 상관분석에 있어 성성숙 일령 및 체중과는 정(+)의 상관을, 산란수 및 난중과는 약한 부(-)의 상관관계를 나타내었다. 이러한 결과는 telomere 함유율이 닭의 생명표지 및 생산능력의 표지로서의 개발 가능성을 시사한다 하겠다.

• Archives of Pharmacal Research
• /
• 제29권8호
• /
• pp.712-719
• /
• 2006

In this study, we prepared core-shell type nanoparticles of a poly(DL-lactide-co-glycolide) (PLGA) grafted-dextran (DexLG) copolymer with varying graft ratio of PLGA. The synthesis of the DexLG copolymer was confirmed by $^1H$ nuclear magnetic resonance (NMR) spectroscopy. The DexLG copolymer was able to form nanoparticles in water by self-aggregating process, and their particle size was around $50\;nm{\sim}300\;nm$ according to the graft ratio of PLGA. Morphological observations using a transmission electron microscope (TEM) showed that the nanoparticles of the DexLG copolymer have uniformly spherical shapes. From fluorescence probe study using pyrene as a hydrophobic probe, critical association concentration (CAC) values determined from the fluorescence excitation spectra were increased as increase of DS of PLGA. $^1H-NMR$ spectroscopy using $D_2O$ and DMSO approved that DexLG nanoparticles have core-shell structure, i.e. hydrophobic block PLGA consisted inner-core as a drug-incorporating domain and dextran consisted as a hydrated outershell. Drug release rate from DexLG nano-particles became faster in the presence of dextranase in spite of the release rate not being significantly changed at high graft ratio of PLGA. Core-shell type nanoparticles of DexLG copolymer can be used as a colonic drug carrier. In conclusion, size, morphology, and molecular structure of DexLG nanoparticles are available to consider as an oral drug targeting nanoparticles.

• Biomaterials and Biomechanics in Bioengineering
• /
• 제3권3호
• /
• pp.129-140
• /
• 2016

This article reports the development of biodegradable photoluminescent polymer (BPLP)-based nanoparticles (NPs) incorporating either magnetic nanoparticles (BPLP-MNPs) or gadopentate dimeglumine (BPLP-Gd NPs), for cancer diagnosis and treatment. The aim of the study is to compare these nanoparticles in terms of their surface properties, fluorescence intensities, MR imaging capabilities, and in vitro characteristics to choose the most promising dual-imaging nanoprobe. Results indicate that BPLP-MNPs and BPLP-Gd NPs had a size of $195{\pm}43nm$ and $161{\pm}55nm$, respectively and showed good stability in DI water and 10% serum for 5 days. BPLP-Gd NPs showed similar fluorescence as the original BPLP materials under UV light, whereas BPLP-MNPs showed comparatively less fluorescence. VSM and MRI confirmed that the NPs retained their magnetic properties following encapsulation within BPLP. Further, in vitro studies using HPV-7 immortalized prostate epithelial cells and human dermal fibroblasts (HDFs) showed > 70% cell viability up to $100{\mu}g/ml$ NP concentration. Dose-dependent uptake of both types of NPs by PC3 and LNCaP prostate cancer cells was also observed. Thus, our results indicate that BPLP-Gd NPs would be more appropriate for use as a dual-imaging probe as the contrast agent does not mask the fluorescence of the polymer. Future studies would involve in vivo imaging following administration of BPLP-Gd NPs for biomedical applications including cancer detection.