• 한국자동차공학회논문집
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• 제5권6호
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• pp.167-174
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• 1997

The penetration depth and the size distribution of the droplets of fuel sprays are important in the operation of spark-ignition MPI engines. A fluorescence/scattering image technique for droplet sizing was applied to measure th edroplet size distribution in non-evaporating gasoline sprays. The fluorescence and scattering lights were imaged simultaneously by the two-dimensional visualization system composed of a laser sheet, a doubling prism, optical filters, and a CCD camera. Quantitative droplet size distributions were extracted from evaluating the ratio of the two light densities. The mean droplet size measured by the fluorescence/scattering technique was compared with the result obtained by the enlarged photographs of droplets. The fluorescence/scattering image technique also gives the useful information of the characteristics of droplet impingement in a inclined wall.

• 한국가시화정보학회지
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• 제21권3호
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• pp.65-74
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• 2023

Compared to epifluorescence(EPI) microscopy which captures fluorescence from the entire depth of sample, total internal reflection fluorescence(TIRF) can selectively visualize only a single surface of it. TIRF uses a thin evanescent field generated by the total internal reflection of laser light on surface. However, conventional TIRF system are designed for total internal reflection to occur at the upper surface of sample, making them unsuitable for sessile droplet imaging. We designed a TIRF system suitable for a sessile droplet imaging by utilizing slide glass as a lightguide. We presented the details for constructing the TIRF system using a prism, slide glass, air slit, and optical trap. Then, we compared the TIRF with EPI by imaging the droplet with fluorescent particles during its drying process. As a result, TIRF allows us to distinctly visualize the drying pattern on the bottom surface of droplet.

• 대한기계학회논문집B
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• 제25권6호
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• pp.868-875
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• 2001

The objective of this study is to investigate the average droplet size distributions of a GDI spray by simultaneous fluorescence/scattering image technique. GDI engine is recently very popular because of high engine efficiency and low emissions. However, the injectors must have good spray characteristics because the fuel is directly injected into the cylinder. The fuel mixtures used in this study were 2% of fluorobenzene, 9% of DEMA(diethyl-methyl-amine) and 89% of hexane by volume. The system for obtaining 2-D fluorescence/scattering images of fuel spray was constituted of a laser sheet, a doubling prism, optical filters, and an ICCD camera. Using the ratio of the fluorescence to the scattering intensities, SMD distributions were obtained. SMD measured by the technique was compared with that obtained by PDA. It was found that average droplet size was bigger at spray center in the early stage of injection and at the outer periphery of the spray in the late stage of injection.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2008년 영문 학술대회
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• pp.486-491
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• 2008

The spray characteristics and drop size measurements have been experimentally studied in liquid jets injected into subsonic crossflow. With water as fuel injection velocity, injection angle and atomizer internal flows were varied to provide of jet operation conditions. The injector internal flow was classified as three modes such as a non-cavitation flow, cavitation, and hydraulic flip flows. Pulsed Shadowgraph Photography measurement was used to determine the spatial distribution of the spray droplet diameter in a subsonic crossflow of air. And this study also obtains the SMD(Sauter Mean Diameters) distribution by using PLLIF(Planar Liquid Laser Induced Fluorescence) technique. The objectives of this research are getting a droplet distribution and drop size measurement of each condition and compare with the other flows effect. As the result, This research have been showed the droplet size were spatially dependent on air-stream velocity, fuel injection velocity, injection angle effects and normalized distance from the injector exit length.(x/d, y/d)There are also different droplet size characteristics between cavitation, hydraulic flip and the non-cavitation flows.

• 한국분무공학회지
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• 제12권2호
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• pp.115-122
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• 2007

The spray characteristics and drop size measurements have been experimentally studied in liquid jets injected into subsonic crossflow. With water as fuel injection velocity, injection angle and atomize. internal flows were varied to provide of jet operation conditions. The injector internal flow was classified as three modes such as a non-cavitation flow, cavitation, and hydraulic flip flows. Pulsed Shadowgraph Photography measurement was used to determine the spatial distribution of the spray droplet diameter in a subsonic crossflow of air. And this study also obtains the SMD (Sauter Mean Diameters) distribution by using Planar Liquid Laser Induced Fluorescence technique. The objectives of this research are get a droplet distributions and drop size measurements of each condition and compare with the other flow effects. As the result, This research has been showned that droplet size were spatially dependent on air-stream velocity, fuel injection velocity, injection angle effects, and normalized distance from the injector exit length(x/d, y/d). There are also different droplet size characteristics between cavitation, hydraulic flip and the non-cavitation flows.

• 한국자동차공학회논문집
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• 제12권1호
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• pp.25-31
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• 2004

Two-dimensional laser visualization methods have been used in the study of breakup and atomization processes of non-evaporating diesel sprays. A single-hole spray injected into a quiescent atmospheric environment was visualized by the LIF(Laser Induced Fluorescence) and scattering technique. The LIF technique could be implemented to take the images which are magnified enough to show the shape of liquid ligaments and small droplets. The spontaneous scattering and fluorescent images of sprays were also taken to investigate the atomization of droplets. In the tip and periphery of a spray. the scattering light is bright and the ratio of fluorescent/scattering intensity is lower. This characteristics indicate the very high number density of small droplets which are well atomized.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2000년도 추계학술대회논문집B
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• pp.353-358
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• 2000

To achieve the requirement for high fuel economy and low emissions, the research for GDI engines is recently very brisk in the whole world. This study was performed to measure distribution of average particle size in non-evaporating spray. The 2-D fluorescence/scattering images of fuel spray were captured simultaneously by visualization system composed of a laser sheet, a doubling prism, optical filters, and an ICCD camera. Using the ratio of the two light intensities, particle size distribution was obtained. The SMD measured by fluorescence/scattering technique was compared with it obtained by PDA. The experimental results show that the spray structure of GDI injector and temporal SMD distribution.

• 한국연소학회지
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• 제7권4호
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• pp.1-9
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• 2002

The morphology of deposits on $15-{\mu}m$ thin SiC filaments has been investigated with SEM and compared with UV-excited laser induced broadband fluorescences in co-flowing, propane laminar diffusion flames in a reduced oxidizer environment. The homogeneous morphology of droplet-like deposits inner flame zone and the agglomeration of condensed-phase deposits and the transition to soots from grown up droplet-like precursors with approaching the flame surface can be observed in a barely sooting flame. The average size of the mature soots deposited in the luminous flame edge is scarcely dependent on their axial position in a confined flame under reduced oxidizer condition. A double structure of PAH fluorescence is observed in near-extinction flames with further decreasing of oxidizer. A comparison of the PAH fluorescence with the morphologies of deposits indicates that appearance of the "dark" hollow zone is caused by a decreased number density of developed liquid-phase large molecules and the outer weak fluorescence zone is caused by the diffusion of gas-phase small molecules.

• 한국가시화정보학회지
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• 제15권1호
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• pp.25-31
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• 2017

This study aims to visualize the Marangoni flow inside a droplet placed on heated hydrophobic surface and to measure its internal velocity field. The experimental result shows that the internal velocity increases with the increase of the plate temperature. In addition, the temperature difference induces the initial flow and drives the Marangoni circulation inside the droplet as soon as the evaporation starts (i.e. the thermal Marangoni flow). The fluorescence particles in the droplet trace two large-scale counter-rotating vortex pairs yielding the downwards flow along the vertical central axis. These vortex pairs gradually become small and move towards the contact line as time goes by, and this Marangoni flow sustains only for a half of the total evaporation time.

• Korean Chemical Engineering Research
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• 제61권2호
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• pp.247-257
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• 2023

본 연구에서는 이미지 분석 프로그램을 통해 액적 내 박테리아 세포의 개수를 측정하는 코딩-기반의 자동화된 세포 계수 방법을 제시하였다. 먼저, 형광 이미지 기반의 분석을 위하여, 형광단백질을 발현하는 균주를 담지한 액적을 형성하고 이를 광학 및 형광 현미경을 이용하여 분석 결과를 나타냈다. 액적의 관찰을 용이하게 위하여 유리 그리드에 도포하고, 촬영한 광학 이미지를 통해 분석하고자 하는 영역(Region of Interest)을 지정하였다. 동일한 위치에서 촬영한 형광 이미지에서 앞서 지정된 영역 속 특정 임계 값을 넘는 형광 신호를 계수하여 세포 수를 정량화 하였다. 또한 서로 다른 농도의 항생제를 처리한 액적 내 박테리아의 시간에 따른 세포 개수 변화의 차이를 추적하였다. 30분 간격으로 동일한 위치에서의 형광 이미지들을 동시에 분석함으로써 시간에 따른 세포 개수 변화를 도출하였고, 본 계수법의 성능을 실험적으로 검증하였다. 본 논문의 방법은 외부 분석 프로그램을 이용한 기존 방법 대비 분석 시간을 15배 가량 단축하고, 99%의 정확도를 보이는 것으로 확인되었다. 더 나아가 사용자의 연구의 방향에 맞춰 제시된 코드의 확장 수정을 통해 다양한 종류의 세포 계수 연구에 도움이 될 것으로 기대된다.