• Journal of Photoscience
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• v.11 no.32
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• pp.47-53
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• 2004

A laser scanning fluorescence microscope system has been fabricated for single molecule detection (SMD). Problems associated with the system set-up have been discussed along with proper suggestions. Based on the SMD results obtained by using the apparatus, a statistical method has been suggested to determine the minimum number of required molecules to form a group of uniform average in a selected error range.

• Bulletin of the Korean Chemical Society
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• v.20 no.10
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• pp.1165-1171
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• 1999

The analysis of trace herbicides using the on-line SPE-HPLC system and a photochemical reaction was studied. 18 compounds of herbicides including eight triazines, six phenoxy acids and esters, and four other herbicides were examined. The on-line SPE-HPLC system developed for selection of eluting solvent improved chromatographic efficiency. The recoveries of herbicides were higher than 77%. With 100 mL tap water samples, the detection limits for all analytes were in the 0.1-2.3×10-10 M range. Detection was done by a UV or fluorescence spectrometer after photochemical reaction at the end of the column with 2W or 450W mercury lamp. Without a photochemical reaction, all compounds responded to 230 nm UV detector, but phenoxy acids and esters were weakly detected. However, with a photochemical reaction, these compounds were selectively detected at 320 nm wavelength of UV absorption and 400 nm emission of the fluorescence detectors. This method can be used for the analysis of environmental water containing herbicides at trace levels.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2011.06a
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• pp.835-836
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• 2011

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.736-736
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• 2003

• Bulletin of the Korean Chemical Society
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• v.29 no.1
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• pp.130-134
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• 2008

Carbohydrate-conjugated chlorins were synthesized for use as biosensors for the detection of the galectin-3 cancer marker. We used ELISA, SDS-gel electrophoresis, and Bradford assays to examine the binding of galectins to d-(+)-galactose- and b-lactose-conjugated chlorins. The binding affinities of these conjugated chlorins for galectin-3 were quantified using fluorescence spectroscopy. The fluorescence emission of the carbohydrate-conjugated chlorins decreased as the amount of galectin-3 in the binding reaction increased over a limited concentration range, indicating that carbohydrate-conjugated chlorins are potentially useful fluorescence biosensors for the galectin-3 cancer marker.

• The Transactions of the Korean Institute of Electrical Engineers D
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• v.52 no.11
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• pp.634-643
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• 2003

This paper presents a method for detecting skin tumors on chicken carcasses using hyperspectral images. It utilizes both fluorescence and reflectance image information in hyperspectral images. A detection system that is built on this concept can increase detection rate and reduce processing time, because the procedure for detection can be simplified. Chicken carcasses are examined first using band ratio FCM information of fluorescence image and it results in candidate regions for skin tumor. Next classifier selects the real tumor spots using PCA components information of reflectance image from the candidate regions. For the real world application, real-time processing is a key issue in implementation and the proposed method can accommodate the requirement by using a limited number of features to maintain the low computational complexity. Nevertheless, it shows favorable results and, in addition, uncovers meaningful spectral bands for detecting tumors using hyperspectral image. The method and findings can be employed in implementing customized chicken tumor detection systems.

• Current Optics and Photonics
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• v.3 no.3
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• pp.256-262
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• 2019

Extensive tumor resection accompanied by radiotherapy and chemotherapy is the standard of care for malignant gliomas. However, there is a significant obstacle to the complete resection of the tumor due to the difficulty of distinguishing tumor and normal brain tissue with a conventional surgical microscope. Recently, multiple studies have shown the possibility of fluorescence-guided surgery in malignant gliomas. The most used fluorescence dyes for brain tumor surgery are 5-aminolevulinic acid (5-ALA) and indocyanine green (ICG). In this paper, a new fluorescence guided operation system, which can detect both 5-ALA and ICG fluorescent images simultaneously, is presented. This operation system consists of light emitting diodes (LEDs) which emits 410 nm and 740 nm wavelengths. We have performed experiments on rats in order to verify the operation of the newly developed operation system. Oral administration and imaging were performed to observe the fluorescence of 5-ALA and ICG fluorescence in rats. When LEDs at wavelengths of 410 nm and 740 nm were irradiated on rats, 628 nm wavelength with a violet fluorescence color and 825 nm wavelength with a red fluorescence color were expressed in 5-ALA and ICG fluorescent material, respectively, thus we were able to distinguish the tumor tissues easily. Previously, due to the poor resolution of the conventional surgical microscope and the fact that the color of the vein is similar to that of the tumor, the tumor resection margin was not easy to observe, thus increasing the likelihood for cancer recurrence. However, when the tumor is observed through the fluorescence guided operation system, it is possible to easily distinguish the color with the naked eye and it can be completely removed. Therefore, it is expected that surgical removal of cancerous tumors will be possible and surgical applications and surgical microscopes for cancer tumor removal surgery will be promising in the future.

• Journal of Dairy Science and Biotechnology
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• v.33 no.4
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• pp.271-275
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• 2015

Colloidal semiconductor CdSe-ZnS core-shell nanocrystal quantum dot (Qdot) are luminescent inorganic fluorophores that show potential to overcome some of the functional limitations encountered with organic dyes in fluorescence labeling applications. Salmonella Enteritidis has emerged as a major cause of human salmonellosis worldwide since the 1980s. A rapid, specific, and sensitive method for the detection of Salmonella Enteritidis was developed using Qdot as a fluorescence marker coupled with immunomagnetic separation. Magnetic beads coated with anti-Salmonella Enteritidis antibodies were employed to selectively capture the target bacteria, and biotin-conjugated anti-Salmonella antibodies were added to form sandwich immune complexes. After magnetic separation, the immune complexes were labeled with Qdot via biotin-streptavidin conjugation, and fluorescence measurement was carried out using a fluorescence measurement system. The detection limit of the Qdot method was a Salmonella Enteritidis concentration of $10^3$ colony-forming units (CFU)/mL, whereas the conventional fluorescein isothiocyanate-based method required over $10^5CFU/mL$. The total detection time was within 2 h. In addition to the potential for general nanotechnology development, these results suggest a new rapid detection method of various pathogenic bacteria from a complex food matrix.

• Journal of Biosystems Engineering
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• v.34 no.1
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• pp.37-43
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• 2009

Hyperspectral reflectance and fluorescence scattering have been researched recently for measuring fruit post-harvest quality and condition. And they are promising for nondestructive detection of fruit quality. The objective of this research was to develop a model, which measure the quality of apple by using hyperspectral reflectance and fluorescence. A violet laser (408 nm) and a quartz tungsten halogen light were used as light sources for generating laser induced fluorescence and reflectance scattering in apples, respectively. The laser induced fluorescence and reflectance of 'Golden Delicious' apples were measured by using a hyperspectral imaging system. Fruit firmness, soluble solids and acid content were measured using standard destructive methods. Principal component analyses were performed to extract critical information from both hyperspectral reflectance and fluorescence data and this information was then related to fruit quality indexes. The fluorescence models had poorer predictions of the three quality indexes than the reflectance models. However, the prediction models of integrating fluorescence and reflectance performed consistently better than the individual models of either reflectance or fluorescence. The correlation coefficient for fruit firmness, soluble solid content, and tillable acidity from the integrated model was 0.86, 0.75, and 0.66 respectively. Also the standard errors were 6.97 N, 1.05%, and 0.07% respectively.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2019.05a
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• pp.278-280
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• 2019

Flow cytometer is one of the instrument that can measure various optical properties of a single cell or microparticle. These parameters including size, granularity, and fluorescence intensity are determined by the physical and optical interaction of the cells with excitation light source. However, users have some difficulties such as high cost, size of instrument, and limited fluorescence selectivity. In addition, abundant data is also unintentionally acquired even though user wants to have a single optical parameter. For these reasons, the use of flow cytometer is more challenging for researchers to apply their study. Therefore, the proposed study aims to develop a low-cost portable fluorescence acquisition system using a commercially available light-emitting diode and photodiode. It is designed by a 3D printer, and fluorescence selectivities are increased by changing of the light source / optical filter / detection sensor. Various number sets of fluorescently labeled cells were measured, and its feasibility was evaluated through the proposed system. As a result, acquried fluorescence intensities were proportional to the concentration of the cells and showed high linearity.