Journal of Photoscience
- Volume 11 Issue 32
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- Pages.47-53
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- 2004
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- 1225-8555(pISSN)
Fabrication of a Single Molecule Detection System and Its Application: Connection between Ensemble and Single Molecule Measurements
- Park, Mira (Optical Nano-Metrology Group, Division of Optical Metrology, Korea Research Institute of Standards & Science) ;
- Lee, Heung Soon (Optical Nano-Metrology Group, Division of Optical Metrology, Korea Research Institute of Standards & Science) ;
- Kim, DongHo (National Creative Research Initiatives Center for Ultrafast Optical Characteristics Control and Department of Chemistry, Yonsei University) ;
- Song, Nam Woong (Optical Nano-Metrology Group, Division of Optical Metrology, Korea Research Institute of Standards & Science)
- Published : 2004.08.01
Abstract
A laser scanning fluorescence microscope system has been fabricated for single molecule detection (SMD). Problems associated with the system set-up have been discussed along with proper suggestions. Based on the SMD results obtained by using the apparatus, a statistical method has been suggested to determine the minimum number of required molecules to form a group of uniform average in a selected error range.