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http://dx.doi.org/10.5012/bkcs.2008.29.1.130

Binding Affinities of Carbohydrate-Conjugated Chlorins for Galectin-3  

Park, Young K. (PDT Research Institute, Department of Nano System Engineering, Inje University)
Bold, Bayarmaa (PDT Research Institute, Department of Nano System Engineering, Inje University)
Cui, Bing Cun (PDT Research Institute, Department of Nano System Engineering, Inje University)
Bai, Jin Quan (PDT Research Institute, Department of Nano System Engineering, Inje University)
Lee, Woo-Kyoung (PDT Research Institute, Department of Nano System Engineering, Inje University)
Shim, Young-Key (PDT Research Institute, Department of Nano System Engineering, Inje University)
Publication Information
Bulletin of the Korean Chemical Society / v.29, no.1, 2008 , pp. 130-134 More about this Journal
Abstract
Carbohydrate-conjugated chlorins were synthesized for use as biosensors for the detection of the galectin-3 cancer marker. We used ELISA, SDS-gel electrophoresis, and Bradford assays to examine the binding of galectins to d-(+)-galactose- and b-lactose-conjugated chlorins. The binding affinities of these conjugated chlorins for galectin-3 were quantified using fluorescence spectroscopy. The fluorescence emission of the carbohydrate-conjugated chlorins decreased as the amount of galectin-3 in the binding reaction increased over a limited concentration range, indicating that carbohydrate-conjugated chlorins are potentially useful fluorescence biosensors for the galectin-3 cancer marker.
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