• Asian Pacific Journal of Cancer Prevention
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• 제13권9호
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• pp.4745-4749
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• 2012

The diagnosis of malignant mesothelioma (MM) remains a clinical challenge and the fluorescence in situ hybridization (FISH) assay has been reported to be one promising tool. The present meta-analysis aimed to establish the overall diagnostic accuracy of FISH for diagnosing MM. After a systematic review of English language studies, the sensitivity, specificity and other measures of accuracy of FISH in the diagnosis of MM were pooled using random-effects models. Summary receiver operating characteristic curves were applied to summarize overall test performance. Nine studies met our inclusion criteria, the pooled sensitivity and specificity for FISH for diagnosing MM being 0.72 (95% CI 0.67-0.76) and 1.00 (95% CI 0.98-1.00), respectively. The positive likelihood ratio was 34.5 (95% CI 14.5-82.10), the negative likelihood ratio was 0.24 (95% CI 0.16-0.36), and the diagnostic odds ratio was 204.9 (95% CI 76.8-546.6), the area under the curve being 0.99. Our data suggest that the FISH assay is likely to be a useful diagnostic tool for confirming MM. However, considering the limited studies and patients included, further large scale studies are needed to confirm these findings.

• 생태와환경
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• 제37권4호통권109호
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• pp.363-367
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• 2004

낙동강 전 수계 8개 정점에서 분자기법인 FISH (Fluorescent in situ Hybridization)법으로 세균군집구조를 비교분석하였다. Eubacteria에 ${\alpha}\;{\cdot}\;{\beta}\;{\cdot}\;{\gamma}-subclasses$ proteobacter CF group 세균의 합이 총세군수에서 차지하는 비율이 9.3-42.5%에서 변화하였고 그 최고치는 히상류, 청량에서 나타났다. 각 세균그룹이 총세균수에서 차지하는 비율이 10% 미만이었으나 최상류에서의 CF그룹이 총세균수에서 차지하는 비율은 23%이었다. 또한 유기물질을 분해해서 빠른 성장을 한다는 ${\gamma}-subclasses$ proteobacteria 세균군이 예상과는 달리 유기오염정도가 높은 하류에 비해 상류에서 더 많이 검출되었다. 아울러 암모니아산화세균은 $2.7-18.0{\times}10^4$ cells $mL^{-1}$의 범위에서 변화하였고 하류에서 최저치를 그리고 최고치는 중류에서 보였다. 반면에 아질산산화세균의 경우, 전수계에 걸쳐 정점간의 별 차이 없이 $5.2-7.7{\times}10^4$ Cells $mL^{-1}$에서 변화하였으며 그들이 총세균수에서 차지하는 비율은 두세균군간의 차이없이 1.0-13.6%에서 변화하였다. 결론적으로 FISH법은 통상적으로 세균군집의 정량적인 분석에 사용되지만 그 결과는 본 연구결과에서 보는 바와 같이 수계 환경의 현황에 관한 좋은 정보를 제공해주기도 한다.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 춘계학술대회 및 국제심포지움 초록집
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• pp.126-126
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• 2005

• Journal of the Optical Society of Korea
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• 제10권3호
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• pp.130-142
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• 2006

Lab-on-a-chip technology is attracting great interest because the miniaturization of reaction systems offers practical advantages over classical bench-top chemical systems. Rapid mixing of the fluids flowing through a microchannel is very important for various applications of microfluidic systems. In addition, highly sensitive on-chip detection techniques are essential for the in situ monitoring of chemical reactions because the detection volume in a channel is extremely small. Recently, a confocal surface enhanced Raman spectroscopic (SERS) technique, for the highly sensitive biological analysis in a microfluidic sensor, has been developed in our research group. Here, a highly precise quantitative measurement can be obtained if continuous flow and homogeneous mixing condition between analytes and silver nano-colloids are maintained. Recently, we also reported a new analytical method of DNA hybridization involving a PDMS microfluidic sensor using fluorescence energy transfer (FRET). This method overcomes many of the drawbacks of microarray chips, such as long hybridization times and inconvenient immobilization procedures. In this paper, our recent applications of the confocal Raman/fluorescence microscopic technology to a highly sensitive lab-on-a-chip detection will be reviewed.

• 생태와환경
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• 제40권4호
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• pp.553-559
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• 2007

The vertical distributions of sulfate reducing bacteria (SRB) in sediments of lakes in Korea (Lake Sihwa and Lake Soyang) and China (Lake Aha and Lake Erhai) were investigated by fluorescence in situ hybridization (FISH). SRB from sediment of Lakes of China were located to deeper layer than those in Lakes of Korea. SRB were not detected below 19 cm and 10 cm depth in sediments of Lake Sihwa and Lake Soyang, respectively. SRB numbers were, however, detected at all observed sediments in Lake Aha and Lake Erhai. In case of lakes in Korea, the proportion of SRB ranged from 2.9 to 25.6% (Lake Sihwa) and ranged from 0.6 to 7.1% (Lake Soyang). For lakes in China, the proportions of SRB were from 0.6 to 19.4% and from 2.9 to 11.2% within sediments from Lake Aha and from Lake Erhai, respectively. The high peaks of SRB numbers in sediments of all lakes were appearing at depths between 0 cm and 2 cm.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.3825-3827
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• 2012

FISH is one of the most sensitive molecular methods to detect genetic abnormalities with DNA probes. When cytogenetic studies are normal or insufficient, FISH may detect cryptic rearrangements, rare or slowly proliferative abnormal populations in non-mitotic cells. We cytogenetically evaluated 70 childhood ALL - 67.1% were found to have an abnormal karyotype. The 23 patients (32.9%) with a normal karyotype were analyzed by FISH applying two probes; TEL/AML1 and MYB which detect cryptic rearrangements of t(12;21)(p13;q22) and deletion of (6q) respectively, associated with a good prognosis. Out of 23 patients, one was positive for t(12;21)(p13;q22) (4.3%). None of our patients were positive for MYB del(6q). Two patients showed an extra signal for MYB on chromosomes other than 6 (8.6 %) indicating amplification or duplication. Findings were compared with the available literature. Our study clearly indicated the integrated FISH screening method to increase the abnormality detection rate in a narrow range. FISH is less useful for diagnostic study of patients with suspected del(6q) but it helps in detecting known cryptic rearrangements as well as identification of new abnormalities(translocation , duplication and amplification) at the gene level.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권6호
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• pp.482-489
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• 2004

Bacillus species were observed and quantified by molecular approaches, using the 16S rDNA primers/probes, in a wastewater treatment plant designed for the purpose of stimulating the growth of Bacillus species. The plant has been operating as a test plant since 1997 in the city of Ina, Japan, with excellent treatment performance. Observations by in situ hybridization, using Bacillus-specific probes, indicated that Bacillus strains were inhabited in the plant and their num­bers decreased during the treatment process. Similar results were obtained from a quantitative PCR analysis using a Bacillus-specific primer set, and the amount of DNA originating from various Bacillus species was maximally $1.91%\$ of the total DNA in the wastewater treatment tank. Clone library analysis using the Bacillus-specific primers suggested that, while the population was no­ticeably increased, the phylogenetic diversity of the increasing Bacillus species was very low.

• 대한소아치과학회지
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• 제31권2호
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• pp.314-322
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• 2004

글루캔 형성은 이온, 완충액 및 영양물질 등과 같은 구강 내 존재하는 물질과 이들의 변화에 영향을 받는다. 본 연구에서는 치아 우식증의 중요한 원인균인 Streptococcus mutans를 실험균으로 하여 수용성 글루캔 합성 효소인 GTFD 유전자의 발현에 대한 이온 및 완충액의 영향을 Fluorescent in situ hybridization 방법으로 관찰하여 다음의 결과를 얻었다. 1. $CaCl_2$ 농도가 1.0mM, KCl 농도가 2.5mM, $MgCl_2$의 농도가 0.4mM일 때 생균수가 가장 많았다. 2. 완충액의 경우 sodium bicarbonate 10mM, sodium phosphate 1mM, potassium phosphate 0.1mM에서 생균수가 가장 많았고, 3가지 완충액 모두 100mM 농도에서 생균수가 가장 적었다. 3. 자당이 함유되지 않은 BHI 배지에 비해 1%자당을 첨가한 경우 gtfD 유전자의 mRNA 발현에 따른 녹색형광이 관찰되었다. 4. $CaCl_2$ 0.25mM일 때 gtfD 유전자의 mRNA 발현에 따른 녹색형광이 대조군보다 강하였고, KCl의 경우 10mM일 때 대조군과 유사한 녹색형광을 나타냈으나, 다른 농도에서는 거의 관찰되지 않았다. $MgCl_2$의 경우 각 농도에서 대조군보다 녹색형광이 약하게 나타났다. 5. Sodium bicarbonate, sodium phosphate 완충액의 경우 mRNA 발현에 따른 녹색형광이 각 농도에서 대조군과 유사하게 나타났으며, potassium phosphate 완충액의 경우 100mM에서 녹색형광을 거의 관찰할 수 없었다.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1998년도 The 12th Symposium on Plant Biotechnology Vol.12
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• pp.61-70
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• 1998

Genome and chromosome analyses in plants using fluorescence in situ hybridization (FISH) and immuno-staining (IMS) methods are reviewed by presenting the recent results obtained by the Chromosome Link, a group of chromosome and genome researchers. FISH is now effective to detect unique nucleotide sequences with 153 bp on the extended DNA fibers. Genomic in situ hybridization (GISH) also allows painting plant chromosomes of different genomes. GISH is quite effective to detect the genomic differentiation in the individual chromosomes within a nucleus. Three dimensional (3D) analyses are now available by confocal microscopy and a deconvolution system. These techniques are invaluable to visualize both the structural and functional dynamics within a nucleus. 3D-FISH revealed the spatial differentiation of different genomees within a nucleus. 3D-FISH also proved structural partition of centromeric and telomeric domains within a barely nucleus. The dynamic acetylation of histone H4 at the specific regions of a genome during a cell cycle is also analyzed using 3D-IMS. It is anticipated that these methods will provide us powerful tools to understand the structural and functional significance of plant chromosomes and genomes.

• Animal Bioscience
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• 제35권9호
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• pp.1289-1302
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• 2022

The next generation sequencing has significantly contributed to clarify the genome structure of many species of zootechnical interest. However, to date, some portions of the genome, especially those linked to a heterogametic nature such as the Y chromosome, are difficult to assemble and many gaps are still present. It is well known that the fluorescence in situ hybridization (FISH) is an excellent tool for identifying genes unequivocably mapped on chromosomes. Therefore, FISH can contribute to the localization of unplaced genome sequences, as well as to correct assembly errors generated by comparative bioinformatics. To this end, it is necessary to have starting points; therefore, in this study, we reviewed the physically mapped genes on the Y chromosome of cattle, buffalo, sheep, goats, pigs, horses and alpacas. A total of 208 loci were currently mapped by FISH. 89 were located in the male-specific region of the Y chromosome (MSY) and 119 were identified in the pseudoautosomal region (PAR). The loci reported in MSY and PAR were respectively: 18 and 25 in Bos taurus, 5 and 7 in Bubalus bubalis, 5 and 24 in Ovis aries, 5 and 19 in Capra hircus, 10 and 16 in Sus scrofa, 46 and 18 in Equus caballus. While in Vicugna pacos only 10 loci are reported in the PAR region. The correct knowledge and assembly of all genome sequences, including those of genes mapped on the Y chromosome, will help to elucidate their biological processes, as well as to discover and exploit potentially epistasis effects useful for selection breeding programs.