• Asian Pacific Journal of Cancer Prevention
• /
• 제15권16호
• /
• pp.6627-6632
• /
• 2014

Purpose: The present study concerns molecular mechanisms involved in induction of apoptosis by a fungal taxol extracted from the fungus Cladosporium oxysporum in T47D human breast cancer cells. Materials and Methods: Apoptosis-induced by the fungal taxol was assessed by MTT assay, nuclear staining, DNA fragmentation, flow cytometry and pro- as well as anti-apoptotic protein expression by Western blotting. Results: Our results showed inhibition of T47D cell proliferation with an $IC_{50}$ value of $2.5{\mu}M/ml$ after 24 h incubation. It was suggested that the extract may exert its anti-proliferative effect on human breast cancer cell line by suppressing growth, arresting through the cell cycle, increase in DNA fragmentation as well as down-regulation of the expression of NF-${\kappa}B$, Bcl-2 and Bcl-XL and up-regulation of pro-apoptotic proteins like Bax, cyt-C and caspase-3. Conclusions: We propose that the fungal taxol contributes to growth inhibition in the human breast cancer cell through apoptosis induction via a mitochondrial mediated pathway, with possible potential as an anticancer therapeutic agent.

• 대한기계학회논문집
• /
• 제12권3호
• /
• pp.551-560
• /
• 1988

본 연구에서는 해석하려는 시스템의 유동 및 열전달 현상의 개념도를 Fig.1 에 나타내었다. 고체 입자는 윗부분 홈으로부터 분사되어, 선택적 투과면을 통해서 입사되는 복사열을 흡수 하며, 기체는 아래 또는 위의 홈 부분으로부터 들어와서 고체 입자와의 대류열전달로 가열이 된다. 기차게 아래 홈에서부터 분사되는 경우 대류에 의해 가열된 기체가 역성층화로 인해 부력을 받게 되어, 고체 입자의 하강 속도가 감 소할 때 입자의 체류 시간의 증가에 따른 복사열의 흡수효과에 대하여 고찰하였으며 입자의 크기, 투사 복사량, 분사속도, 입자의 질량유량 등을 파라미터로 하여 이들의 변화에 따른 영향을 규명하였다. 2-방연계를 고려한 2-방정식 모델을 구성하고 고체 입자에 대하여는 Lagrangian 방법으로 기술하였으며 수치해석에 있어 유한차분법을 도 입하고 두 상간의 상호연계는 PSI-Cell 방법을 이용하였고 복사 열유속은 2-유속 모델 (two-flux model)을 도입하여 계산하였다.

• 한국추진공학회:학술대회논문집
• /
• 한국추진공학회 2008년 영문 학술대회
• /
• pp.659-665
• /
• 2008

The 1-D numerical study of the interior ballistics has been conducted. The unsteady compressible 1-D CFD code using SIMPLER algorithm and QUICK scheme has been developed. The mathematical model of the two-phase flow has been established for the behavior of the interior ballistics. The moving boundary due to the projectile motion as the physical phenomena of the interior ballistics results in the varied control volume. In order to analyze the moving boundary, the numerical codes, which apply the ghost-cell extrapolation method and the Lagrangian method respectively, have been developed. The ghost-cell extrapolation method has been used in the Eulerian coordinate system. The Lagrangian method has been used in Non-Eulerian coordinate system. These codes have been verified through the analysis of the free piston motion problem in the tube. Through this study, the basic techniques of the numerical code for the multi-dimensional two-phase flow of the interior ballistics have been obtained.

• Archives of Plastic Surgery
• /
• 제50권6호
• /
• pp.568-572
• /
• 2023

Cutaneous squamous cell carcinoma (CSCC) overlying an arteriovenous fistula (AVF) is rare and presents unique challenges. This case report describes a method of fistula preservation after CSCC excision using a flow-through venous free flap. The saphenous vein of the venous flap was used as flow-through segment for AVF preservation. The flap was inserted along the dorsal aspect of the forearm wound and microvascular anastomosis of the arterial inflow was completed using a vein just proximal to the radiocephalic fistula anastomosis. Venous outflow was established by creating an end-to-end vascular anastomosis between the cephalic vein and the greater saphenous vein. A separate subcutaneous vein was used to provide a low-pressure outflow for the flap to avoid congestion. This case demonstrates an option for AVF preservation that has not been previously described. It also highlights the importance of a multidisciplinary approach for the safe treatment of CSCCs overlying AVFs.

• 한국수소및신에너지학회논문집
• /
• 제28권6호
• /
• pp.675-682
• /
• 2017

New government, the market for stationary fuel cell systems in domestic is expected to expand in line with the policy for expanding new and renewable energy. In order to promote and expand the domestic market for stationary fuel cell systems, it is required to do research and develop for cost reduction and efficiency improvement technologies through the localization of BOP. In this study, the safety performance including the power consumption, flow rate, noise and air-tightness of the domestic fuel booster blower and the foreign fuel booster blower was evaluated and the performance improvement of the domestic blower was confirmed. As a result of the power consumption measurement and the flow rate according to the back pressure of the A company 2nd prototype and B company, the values were 73 W, 27 LPM, and 55 W, 25 LPM. These results are attributed to the improvement of performance through design changes such as CAM angle and diaphragm material.

• 한국응용과학기술학회지
• /
• 제27권4호
• /
• pp.415-420
• /
• 2010

This study is focused on the channel design of bipolar plate in the electrode of hydrogen gas generator. The characteristics of hydrogen gas generation was studied in view of efficiency of hydrogen gas generation rate and a tendency of gas flow through the riv design of electrode. Since the flow rate and flow pattern of generated gas in the two phase flow system are the most crucial in determining the efficiency of hydrogen gas generator, we adopted the commercial analytical program of COMSOL MultiphysicsTM to calculate the theoretical flow rate of hydrogen gas from the outlet of gas generator and flow pattern of two phase fluid in the electrode. In this study, liquid electrolyte flows into the bipolar plate and decomposed into gas phase, two phase flow simulation is applied to measure the efficiency of hydrogen gas generation.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권4호
• /
• pp.2325-2331
• /
• 2013

Objective: To detect effects of plumbagin on proliferation and apoptosis in non-small cell lung cancer cell lines, and investigate the underlying mechanisms. Materials and Methods: Human non-small cell lung cancer cell lines A549, H292 and H460 were treated with various concentrations of plumbagin. Cell proliferation rates was determined using both cell counting kit-8 (CCK-8) and clonogenic assays. Apoptosis was detected by annexin V/propidium iodide double-labeled flow cytometry and TUNEL assay. The levels of reactive oxygen species (ROS) were detected by flow cytometry. Activity of NF-${\kappa}B$ was examined by electrophoretic mobility shift assay (EMSA) and luciferase reporter assay. Western blotting was used to assess the expression of both NF-${\kappa}B$ regulated apoptotic-related gene and activation of p65 and $I{\kappa}B{\kappa}$. Results: Plumbagin dose-dependently inhibited proliferation of the lung cancer cells. The IC50 values of plumbagin in A549, H292, and H460 cells were 10.3 ${\mu}mol/L$, 7.3 ${\mu}mol/L$, and 6.1 ${\mu}mol/L$ for 12 hours, respectively. The compound concentration-dependently induced apoptosis of the three cell lines. Treatment with plumbagin increased the intracellular level of ROS, and inhibited the activation of NK-${\kappa}B$. In addition to inhibition of NF-${\kappa}B$/p65 nuclear translocation, the compound also suppressed the degradation of $I{\kappa}B{\kappa}$. ROS scavenger NAC highly reversed the effect of plumbagin on apoptosis and inactivation of NK-${\kappa}B$ in H460 cell line. Treatment with plumbagin also increased the activity of caspase-9 and caspase-3, downregulated the expression of Bcl-2, upregulated the expression of Bax, Bak, and CytC. Conclusions: Plumbagin inhibits cell growth and induces apoptosis in human lung cancer cells through an NF-${\kappa}B$-regulated mitochondrial-mediated pathway, involving activation of ROS.

• 한국유체기계학회 논문집
• /
• 제11권6호
• /
• pp.54-63
• /
• 2008

A study on the flow characteristics in a 4-stage axial compressor and the behavior of rotating stall was experimentally performed at the third-stage rotor and stator rows in order to investigate its performance and instability of the compression system. The pressure losses generated due to the leakage flow at a tip clearance and a shroud seal clearance and the wake flow near the trailing edge of a blade were taken into consideration to estimate the causes of performance drop of the low speed research compressor(LSRC) in Seoul national university. In addition, the measurement of rotating stall was conducted with hot-wire probes and the existence and propagation of stall cell could be confirmed through fast Fourier transform and cross-correlation analysis.

• 한국응용과학기술학회지
• /
• 제27권1호
• /
• pp.61-69
• /
• 2010

This study is focused on the modeling of two phase fluid flow system in the electrode of hydrogen gas generator. The characteristics of hydrogen gas generation was studied in view of efficiency of hydrogen gas generation rate and a tendency of gas flow through the riv of electrode. Since the flow rate of generated gas is the most crucial in determining the efficiency of hydrogen gas generator, we adopted the commercial analytical program of COMSOL $Multiphysics^{TM}$ to calculate the theoretical flow rate of hydrogen gas from the outlet of gas generator.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2003년도 춘계학술대회
• /
• pp.1961-1965
• /
• 2003

The flow analysis is needed to verify the physical phenomena through interruption processes for improving the capacity and the reliability of gas circuit breakers. Moreover the small current interruption performance of GCBs could be predicted by coupling the flow characteristics with the electric field one. In this paper, the unsteady flow characteristics and the traveling trajectory are depicted with a commercial CFD code, PHOENICS, programmed for moving motion of objects. In order to validate computational results, the measured pressure data in cylinder and in front of arcing contact are compared with the test results of small current interruption.