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http://dx.doi.org/10.7314/APJCP.2014.15.16.6627

Fungal Taxol Extracted from Cladosporium oxysporum Induces Apoptosis in T47D Human Breast Cancer Cell Line  

Raj, Kathamuthu Gokul (Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University)
Sambantham, Shanmugam (Department of Genetics, Dr. ALMPGIBMS, University of Madras)
Manikanadan, Ramar (Department of Zoology, University of Madras)
Arulvasu, Chinnansamy (Department of Zoology, University of Madras)
Pandi, Mohan (Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.15, no.16, 2014 , pp. 6627-6632 More about this Journal
Abstract
Purpose: The present study concerns molecular mechanisms involved in induction of apoptosis by a fungal taxol extracted from the fungus Cladosporium oxysporum in T47D human breast cancer cells. Materials and Methods: Apoptosis-induced by the fungal taxol was assessed by MTT assay, nuclear staining, DNA fragmentation, flow cytometry and pro- as well as anti-apoptotic protein expression by Western blotting. Results: Our results showed inhibition of T47D cell proliferation with an $IC_{50}$ value of $2.5{\mu}M/ml$ after 24 h incubation. It was suggested that the extract may exert its anti-proliferative effect on human breast cancer cell line by suppressing growth, arresting through the cell cycle, increase in DNA fragmentation as well as down-regulation of the expression of NF-${\kappa}B$, Bcl-2 and Bcl-XL and up-regulation of pro-apoptotic proteins like Bax, cyt-C and caspase-3. Conclusions: We propose that the fungal taxol contributes to growth inhibition in the human breast cancer cell through apoptosis induction via a mitochondrial mediated pathway, with possible potential as an anticancer therapeutic agent.
Keywords
Fungal taxol; apoptosis; DNA fragmentation; DNA damage; NF-${\kappa}B$;
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Times Cited By KSCI : 7  (Citation Analysis)
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1 American Cancer Society. Breast cancer facts and figures 2011- 2012. Atlanta: American cancer society, Inc.
2 Antonsson B, Montessuit S, Sanchez B, Martinou JC (2001). Bax is present as a high molecular weight oligomer/complex in the mitochondrial membrane of apoptotic cells. J Biol Chem, 276, 11615-23.   DOI
3 Antosiewicz HA, Singh SV (2004). Signal transduction pathways leading to cell cycle arrest and apoptosis induction in cancer cells by allium vegetable-derived organosulfur compounds: a review. Mutat Res, 555, 121-31.   DOI
4 Arkin M (2005). Protein-protein interactions and cancer: small molecules going in for the kill. Curr Opin Chem Biol, 9, 317-24.   DOI   ScienceOn
5 BachrachYZ (2012).Contribution of selected medicinal plants for cancer prevention and therapy. Scientific J Faculty of Medicine Nis, 29, 117-23.
6 Cai SX, Drewe J, Kasibhatla S (2006). A chemical genetics approach for the discovery of apoptosis inducers: from phenotypic cell based hts assay and structure-activity relationship studies, to identification of potential anticancer agents and molecular targets. Curr Med Chem, 13, 2627-44.   DOI
7 Chang GC, Hsu SL, Tsai JR, et al (2004). Molecular mechanisms of ZD1839-induced G1-cell cycle arrest and apoptosis in human lung adenocarcinoma A549 cells. Biochem Pharmacol, 68, 1453-64.   DOI   ScienceOn
8 Chen WY, Wu CC, Lan YH, et al (2005). Goniothalamin induces cell cycle-specific apoptosis by modulating the redox status in MDA-MB-231 cells. Eur J Pharmacol, 522, 20-9.   DOI   ScienceOn
9 Cohen JJ (1993). Apoptosis: the physiologic pathway of cell death. Hosp Pract, 28, 35-43.
10 DeVita VT Jr, Hellman S, Rosenberg SA (1997). Cancer. Principles and practice of oncology. Philadelphia, PA: Lippincott-Raven Publishers, 5th edn. pp. 103-119.
11 Doonan F, Cotter XM (2008). Morphological assessment of apoptosis. Methods, 200-4.
12 Elmore S (2007). Apoptosis: a review of programmed cell death. Toxicol Pathol, 35, 495-516.   DOI   ScienceOn
13 Kajimoto S, Takanashi N, Kajimoto T, et al (2002) Sophoranone, extracted from a traditional Chinese medicine Shan Dou Gen, induces apoptosis in human leukemia U937 cells via formation of reactive oxygen species and opening of mitochondrial permeability transition pores. Int J Cancer, 99, 879-90.   DOI   ScienceOn
14 Eskes R, Antonsson B, Osen-Sand A, et al (1998). Bax-induced cytochrome c release from mitochondria is independent of the permeability transition pore but highly dependent on Mg2+ ions. J Cell Biol, 143, 217-24.   DOI   ScienceOn
15 Jemal A, Bray F, Center MM, et al (2011). Global cancer statistics. CA Cancer J Clin, 61, 69-90.   DOI
16 Jurgensmeier JM, Xie Z, Deveraux Q, et al (1998). Bax directly induces release of cytochrome c from isolated mitochondria. Proc Natl Acad Sci USA, 95, 4997-5002.   DOI   ScienceOn
17 Kathiravan G, Sureban SM (2010). Effect of taxol from Pestalotiopsis mangiferae on A549 cells in-vitro study. J Basic and Clinical Pharmacy, 1, 1-8.
18 Kumaran RS, Muthumary J, Hur BK (2009) Isolation and identification of an anticancer drug, taxol from Phyllosticta tabernaemontanae, a leaf spot fungus of an angiosperm, Wrightia tinctoria. J Microbiol, 47, 40-9.   DOI
19 Li T, Xu WS, Wu GS, et al (2014). Platycodin D induces apoptosis, and inhibits adhesion, migration and invasion in HepG2 hepatocellular carcinoma cells. Asian Pac J Cancer Prev, 15, 1745-9.   과학기술학회마을   DOI   ScienceOn
20 Liu D, Huang Z (2011). Synthetic peptides and non-peptidic molecules as probes of structure and function of Bcl-2 family proteins and modulators of apoptosis. Apoptosis, 6, 453-62.
21 Mohsin SK, Weiss HL, Gutierrez MC, et al (2005) Neoadjuvant trastuzumab induces apoptosis in primary breast cancers. J Clin Oncol, 23, 2460-8.   DOI
22 Lowry OH, Rosebrough NJ, Farr AL, Randall RY (1951). Protein measurement with the folin-phenol reagent. J Biol Chem, 193, 265-75.
23 Majno G, Joris I (1995). Apoptosis, Oncosis, and Necrosis An overview of cell death. Am J Pathol, 146, 3-15.
24 Merlin NJ, Parthasarathy V, Santhoshkumar TR (2010). Induction of apoptosis in human breast cancer cell line MCF-7 by phytochemicals from Gmelina asiatica. Afr J Biotechnol, 9, 4451-6.
25 Mosmann T (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods, 65, 55-63.   DOI   ScienceOn
26 Orr GA, Verdier-Pinard P, McDaid H, Horwitz SB (2003). Mechanisms of taxol resistance related to microtubules. Oncogene, 22, 7280-95.   DOI   ScienceOn
27 Pandi M, Kumaran RS, Choi YK, Kim HJ, Muthumary J (2011) Isolation and detection of taxol, an anticancer drug produced from Lasiodiplodia theobromae, an endophytic fungus of the medicinal plant Morinda citrifolia. Afr J Biotechnol, 10, 1428-35.
28 Roos WP, Kaina B (2006). DNA damage-induced cell death by apoptosis. Trends Mol Med, 12, 440-50.   DOI   ScienceOn
29 Rosse T, Olivier R, Monney L, Rager M, Conus S, Fellay I, Jansen B, Borner C (1998). Nature, 391, 496-9.   DOI   ScienceOn
30 Sambantham S, Radha M, Paramasivam A, et al (2013). Molecular mechanism underlying hesperetin-induced apoptosis by in silico analysis and in prostate cancer PC-3 cells. Asian Pac J Cancer Prev, 14, 4347-52.   과학기술학회마을   DOI
31 Vennila R, Thirunavukkarasu SV, Muthumary J (2010) Evaluation of fungal taxol isolated from an endophytic fungus Pestalotiopsis pauciseta VM1 against experimentally induced breast cancer in sprague dawely rats. Res J pharmacol, 4, 38-44.   DOI
32 Steinhoff J, Classen M, Padmavathi PVL, Ishikawa T, Zeth K, Steinhoff HJ, Bordignon E (2010). Oligomerization, and membrane insertion molecular details of bax activation. J Biol Chem. 285, 6636-47.   DOI   ScienceOn
33 Van de Loosdrecht AA, Beelen RH, Ossenkoppele GJ, Broekhoven MG, Langenhuijsen MM (1994). A tetrazoliumbased colorimetric MTT assay to quantitate human monocyte mediated cytotoxicity against leukemic cells from cell lines and patients with acute myeloid leukemia. J Immunol Methods, 174, 311-20.   DOI   ScienceOn
34 Varol U, Yildiz I, Alacacioglu A, Uslu R (2014) Anticancer therapy for breast cancer patients with skin metastases refractory to conventional treatments. Asian Pac J Cancer Prev, 15, 1885-7.   과학기술학회마을   DOI
35 Wan HF, Yu LH, Wu JL, et al (2013). Effect of diallyl trisulfide on human ovarian cancer SKOV- 3/DDP cell apoptosis. Asian Pac J Cancer Prev, 14, 7197-201.   과학기술학회마을   DOI   ScienceOn
36 Wani MC, Taylor HL, Wall ME, Coggon P, McPhail AT (1971). Plant antitumor agents VI: The isolation and structure of taxol, a novel antilekemic and antitumor agent from Taxus brevifolia. J Am Chem Soc, 93, 2325-27.   DOI
37 Xu ZX, Liang J, Haridas V, et al (2007). A plant triterpenoid, avicin D, induces autophagy by activation of AMP-activated protein kinase. Cell Death Differ, 14, 1948-57.   DOI
38 Yang JK (2008). FLIP as an anti-cancer therapeutic target. Yonsei Med J, 49, 19-27.   DOI
39 Alabsi AM, Ali R, Ali Am, et al (2013). Induction of caspase-9, biochemical assessment and morphological changes caused by apoptosis in cancer cells treated with Goniothalamin extracted from Goniothalamus macrophyllus. Asian J cancer Pre, 14, 6273-80.   과학기술학회마을   DOI   ScienceOn
40 Yeung TK, Germond C, Chen X, Wang Z (1999). The mode of action of taxol: Apoptosis at low concentration and necrosis at high concentration. Biochem Biophys Res Commun, 263, 398-404.   DOI   ScienceOn
41 Schiff PB, Fant J, Horwitz SB (1979). Promotion of microtubules assembly in-vitro by taxol. Nature, 277, 665-7.   DOI   ScienceOn
42 Zhang LL, Wu J, Liu Q, et al (2014). MiR-886-5p inhibition inhibits growth and induces apoptosis of MCF7 cells. Asian Pac J Cancer Prev, 15, 1511-5.   과학기술학회마을   DOI   ScienceOn