• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6363-6367
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• 2013

Atractylis lancea (Thunb.) DC. (AL), an important medicinal herb in Asia, has been shown to have anti-tumor effects on cancer cells, but the involved mechanisms are poorly understood. This study focused on potential effects and molecular mechanisms of AL on the proliferation of the Hep-G2 liver cancer cell line in vitro. Cell viability was assessed by MTT test in Hep-G2 cells incubated with an ethanol extract of AL. Then, the effects of AL on apoptosis and cell cycle progression were determined by flow cytometry. Telomeric repeat amplification protocol (TRAP) assays was performed to investigate telomerase activity. The mRNA and protein expression of human telomerase reverse transcriptase (hTERT) and c-myc were determined by real-time RT-PCR and Western blotting. Our results show that AL effectively inhibits proliferation in Hep-G2 cells in a concentrationand time-dependent manner. When Hep-G2 cells were treated with AL after 48h,the $IC_{50}$ was about 72.1 ${\mu}g/mL$. Apoptosis was induced by AL via arresting the cells in the G1 phase. Furthermore, AL effectively reduced telomerase activity through inhibition of mRNA and protein expression of hTERT and c-myc. Hence, these data demonstrate that AL exerts anti-proliferative effects in Hep-G2 cells via down-regulation of the c-myc/hTERT/telomerase pathway.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.129-129
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• 2017

The first key point to the successful pollination and fertilization in plants is the pollen pistil interaction, referring to the cellular and molecular levels, which mainly play active roles in limiting gene flow among maize populations and between maize and teosinte. This study was carried out to identify proteins and investigate the mechanism of gametophytic factors using protein analysis. W22 (ga1); which didn't carry a gametophytic factor and W22 (Ga1), a near iso-genic line were used for the proteome investigation. SDS-PAGE was executed to investigate proteins in the pollen and pistil of W22 (ga1) and W22 (Ga1). A total of 44 differentially expressed proteins were identified in the pollen and pistil on SDS-PAGE using LTQ-FTICR MS. Among the 44 proteins, a total of 24 proteins were identified in the pollen of W22 (ga1) and W22 (Ga1) whereas 20 differentially expressed proteins were identified from the pistil of W22 (Ga) and W22 (Ga1). However, in pollen, 2 proteins were identified only in the W22 (ga1) and 12 proteins only in the W22 (Ga1) whereas 10 proteins were confirmed from the both of W22 (ga1) and W22 (Ga1). In contrary, 10 proteins were appeared only in the pistil of W22 (ga1) and 7 proteins from W22 (Ga1) while 3 proteins confirmed in the both of W22 (ga1) and W22 (Ga1). Moreover, the identified proteins were generally involved in hydrolase activity, nucleic acid binding and nucleotide binding. These results help to reveal the mechanism of gametophytic factors and provide a valuable clue for the pollen and pistil research in maize. In addition, it might provide a comprehensive insight on the proteins that were involved in the regulation of pollen-pistil interaction.

• 환경영향평가
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• 제13권6호
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• pp.285-294
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• 2004

The purpose of this study is to understand the quantitative change of water resources using RMS(Remote Monitoring System) which takes real time data with high reliability. Also, the characteristic of stormwater runoff was understood by the application of the above system for three streams (Jiam, Yulmun, and Gongji stream) in Chuncheon City. The detailed results of these studies are as follows; RMS(Remote Monitoring System) was constructed by the combination of the automatic water-level meter, which measures water-level of streams at all times, and the wireless communication system sending real-time data from the meter. This system is used to evaluate the stormwater runoff in watersheds and the quantitative changes of streams. It is possible to overcome the limit of field investigations needed, which takes a lot of manpower and time, and it is very efficient to provide the reliable flowrate data. Also, it can be applied to the disaster prevention system for flood because the change of flowrate in stream is monitored at real-time. For 3 streams with different watershed characteristics, correlation equations induced from the relation analysis results. In terms of the relation between water-level and flowrate, flowrate was increased rapidly as the water-level rises in case of small watershed and steep slope. The application results of the proposed system for 3 streams (Jiam, Yulmun, Gongji) in Chuncheon city are as follows; The remote monitoring system was very useful for acquisition of the flow rate in stream that are basic data to understand pollutants runoff in watershed. In case of no-rainy day, the runoff ratio for pollutant loading rate was the highest level in Yulmun stream(BOD:2.3%, TN:20.2%, TP:1.2%). So, it shows the management of pollution source is needed such as rehabilitation of sewer line. Runoff ratio of total phosphorus by rainfall in Gongji watershed was increased about 19 times than no-rainy day, which is estimated as the influence of sewer overflow.

• Asian Pacific Journal of Cancer Prevention
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• 제15권8호
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• pp.3471-3476
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• 2014

Background: Aberrant expression of the microRNA-29 family is associated with tumorigenesis and cancer progression. As transport carriers, tumor-derived exosomes are released into the extracellular space and regulate multiple functions of target cells. Thus, we assessed the possibility that exosomes could transport microRNA-29c as a carrier and correlations between microRNA-29c and apoptosis of bladder cancer cells. Materials and Methods: A total of 28 cancer and adjacent tissues were examined by immunohistochemistry to detect BCL-2 and MCL-1 expression. Disease was Ta-T1 in 12 patients, T2-T4 in 16, grade 1 in 8, 2 in 8 and 3 in 12. The expression of microRNA-29c in cancer tissues was detected by quantitative reverse transcriptase PCR (QRT-PCR). An adenovirus containing microRNA-29c was used to infect the BIU-87 human bladder cancer cell line. MicroRNA-29c in exosomes was measured by QRT-PCR. After BIU-87 cells were induced by exosomes-derived microRNA-29c, QRT-PCR was used to detect the level of microRNA-29c. Apoptosis was examined by flow cytometry and BCL-2 and MCL-1 mRNA expressions were assessed by reverse transcription-polymerase chain reaction. Western blotting was used to determine the protein expression of BCL-2 and MCL-1. Results: The expressions of BCL-2 and MCL-1 protein were remarkably increased in bladder carcinoma (p<0.05), but was found mainly in the basal and suprabasal layers in adjacent tissues. The expression of microRNA-29c in cancer tissues was negatively correlated with the BCL-2 and MCL-1. The expression level of microRNA-29c in exosomes and BIU-87 cells from the experiment group was higher than that in control groups (p<0.05). Exosome-derived microRNA-29c induced apoptosis (p<0.01). Although only BCL-2 was reduced at the mRNA level, both BCL-2 and MCL-1 were reduced at the protein level. Conclusions: Human bladder cancer cells infected by microRNA-29c adenovirus can transport microRNA-29c via exosomes. Moreover, exosome-derived microRNA29c induces apoptosis in bladder cancer cells by down-regulating BCL-2 and MCL-1.

• Asian Pacific Journal of Cancer Prevention
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• 제15권2호
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• pp.769-773
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• 2014

Fangchinoline (Fan) inhibits cell proliferation and induces apoptosis in several cancer cell lines. The effects of Fan on cell growth and proliferation in breast cancer cells remain to be elucidated. Here, we show that Fan inhibited cell proliferation in the MDA-MB-231 breast cancer cell line through suppression of the AKT/Gsk-3beta/cyclin D1 signaling pathway. Furthermore, Fan induced apoptosis by increasing the expression of Bax (relative to Bcl-2), active caspase 3 and cytochrome-c. Fan significantly inhibited cell proliferation of MDA-MB-231 cells in a concentration and time dependent manner as determined by MTT assay. Flow cytometry analysis demonstrated that Fan treatment of MDA-MB-231 cells resulted in cell cycle arrest at the G1 phase, which correlated with apparent downregulation of both mRNA and protein levels of both PCNA and cyclin D1. Further analysis demonstrated that Fan decreased the phosphorylation of AKT and GSK-3beta. In addition, Fan up-regulated active caspase3, cytochrome-c protein levels and the ratio of Bax/Bcl-2, accompanied by apoptosis. Taken together, these results suggest that Fan is a potential natural product for the treatment of breast cancer.

• Asian Pacific Journal of Cancer Prevention
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• 제17권7호
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• pp.3213-3222
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• 2016

Background: Methyl donor status influences DNA stability and DNA methylation although little is known about effects on DNA methyltransferases. The aim of this study was to determine whether methyl-donor status influences DNA methyltransferase (Dnmt) gene expression in cervical cancer cells, and if so, whether there are associated effects on global DNA methylation. Materials and Methods: The human cervical cancer cell line, C4-II, was grown in complete medium and medium depleted of folate (F-M+) and folate and methionine (F-M-). Growth rate, intracellular folate, intracellular methionine and homocysteine in the extracellular medium were measured to validate the cancer cell model of methyl donor depletion. Dnmt expression was measured by qRT-PCR using relative quantification and global DNA methylation was measured using a flow cytometric method. Results: Intracellular folate and methionine concentrations were significantly reduced after growth in depleted media. Growth rate was also reduced in response to methyl donor depletion. Extracellular homocysteine was raised compared with controls, indicating disturbance to the methyl cycle. Combined folate and methionine depletion led to a significant down-regulation of Dnmt3a and Dnmt3b; this was associated with an 18% reduction in global DNA methylation compared with controls. Effects of folate and methionine depletion on Dnmt3a and 3b expression were reversed by transferring depleted cells to complete medium. Conclusions: Methyl donor status can evidently influence expression of Dnmts in cervical cancer cells, which is associated with DNA global hypomethylation. Effects on Dnmt expression are reversible, suggesting reversible modulating effects of dietary methyl donor intake on gene expression, which may be relevant for cancer progression.

• 한국수산과학회지
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• 제29권5호
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• pp.722-726
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• 1996

온도변화에 따라 상태가 변하는 가역적 교질특성과 강한 gel화능으로 식품첨가물 뿐만 아니라 공업적으로 널리 쓰이는 한천을 식품산업에서 신소재로서 기능성물질로 개발하기 위하여 제주 우도산 우뭇가사리로 부터 한천을 열수 추출하여 유동학적 특성을 검토하였다. 한천용액은 비뉴우톤 유체로서 농도가 높아짐에 따라 pseudoplastic특성을 강하게 나타내었으며, 한천용액의 유동특성값으로 유동성지수는 $0.5\~5\%$의 범위에서 $80^{\circ}C,\;60^{\circ}C$ 및 $50^{\circ}C$에서 각각 $0.62\~0.69,\;0.46\~0.67$ 및 $0.34\~0.64$이였으며, 농도의 증가에 따라 감소 하였다. 그리고 점조도지수는 $0.12\~1.26\;Pa{\cdot}s^n,\;0.12\~7.28\;Pa{\cdot}s^n$ 및 $3.9\~19952.6\;Pa{\cdot}s^n$이였고, 농도가 증가할수록 그 값이 증가하였다. 점조도지수의 농도의존성은 한천농도 $2\%$를 경계로 기울기가 서로 다른 두개의 직선관계를 보였으며, 온도의존성은 Arrhenius식에 잘 따랐으며, 한천용액의 농도 $0.5\~5\%$ 범위에서 활성화에너지는 $0.09\~13.51\;Kcal/g{\cdot}mol$이였다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권2호
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• pp.685-689
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• 2013

Objective: To study the effects of down-regulation of HDAC6 expression on proliferation, cell cycling and migration of esophageal squamous cell carcinoma (ESCC) cells and related molecular mechanisms. Methods: ESCC cell line EC9706 cells were randomly divided into untreated (with no transfection), control siRNA (transfected with control siRNA) and HDAC6 siRNA (transfected with HDAC6 small interfering RNA) groups. Effects of HDAC6 siRNA interference on expression of HDAC6 mRNA and protein in EC9706 cells were investigated by semi-quantitative RT-PCR, Western blotting and immunocytochemistry methods. Effects of down-regulation of HDAC6 expression on cell proliferation, cell cycle, and cell migration were studied using a CCK-8 kit, flow cytometry and Boyden chambers, respectively. Changes of mRNA and protein expression levels of cell cycle related factor (p21) and cell migration related factor (E-cadherin) were investigated by semi-quantitative RT-PCR and Western blotting methods. Results: After transfection of HDAC6 siRNA, the expression of HDAC6 mRNA and protein in EC9706 cells was significantly downregulated. In the HDAC6 siRNA group, cell proliferation was markedly inhibited, the percentage of cells in G0/G1 phase evidently increased and the percentage of cells in S phase decreased, and the number of migrating cells significantly and obviously decreased. The mRNA and protein expression levels of p21 and E-cadherin in the HDAC6 siRNA group were significantly higher than those in the untreated group and the control siRNA group, respectively. Conclusions: HDAC6 siRNA can effectively downregulate the expression of HDAC6 mRNA and protein in EC9706 cells. Down-regulation of HDAC6 expression can obviously inhibit cell proliferation, arrest cell cycling in the G0/G1 phase and reduce cell migration. The latter two functions may be closely related with the elevation of mRNA and protein expression of p21 and E-cadherin.

• 한국조경학회지
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• 제44권5호
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• pp.12-25
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• 2016

본 연구는 경관을 시간의 개념을 포함한 연속적 시퀀스로 분석하기 위해 연속경관의 무수히 많은 프레임을 추출하여 각 프레임들이 가지고 있는 구성요소들의 시각량을 수치화하여 데이터화한 다음 시퀀스 리듬으로 산출하여 경관계획, 설계 시 필요한 정량적인 데이터로 활용하고자 하였다. 경험자 시선흐름에 근접하도록 동영상을 촬영하기 위해서 연속적 경로인 경관회랑에 따라 경관구역과 경관통제점에 컴퓨터 Motion 기법들을 각각 적용하였다. 동영상에서 일정간격으로 많은 프레임을 추출하여 각 프레임별 경관 구성요소들의 시각량을 연속적으로 산출하기 위해서 CRVP Koo 컴퓨터 프로그램을 개발하여 검증한 다음 대상지에 적용해 보았다. 적용결과, 각 경관의 구성요소별로 수치화된 정량적인 리듬을 추출할 수 있었으며, Photoshop에서 수작업한 결과와 오차도 매우 작았으며, 수작업의 단점을 극복할 수도 있었다. 도출된 시퀀스 리듬은 경관의 경험자 체험에 근접한 것으로 다양한 관점에서 정량적인 분석기법 도출이 가능하였으며, 경관계획이나 설계를 위한 정량적인 데이터와 분석기법으로 활용 가능하였다.

• 대한토목학회논문집
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• 제31권4B호
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• pp.383-391
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• 2011

본 연구에서는 수변생태벨트 조성 사업에 있어서 우선구간을 선정하기 위한 단절구간 평가 방법을 제시하였다. 대상 하천은 홍천강으로 하였으며, 홍천강 약 96 km를 2 km의 구간으로 구분하여, A1-P3의 48개 구간에 대한 도상조사 및 현장조사를 실시하였다. 단절 항목은 크게 연결성 및 생태적 기능성 단절로 구분하였으며, 연결성 단절에서는 토지이용 등의 면적 단절과 도로 및 제방 등의 선적 단절로 구분하였다. 또한 생태벨트의 기능성 단절은 기존 연구결과의 일부 항목을 도입하여 크게 하천 수로 및 하상구조, 수림대 및 식생, 하천구조물로 구분하여 평가하였다. 현장조사를 통하여 각각의 세부 항목별 단절 점수를 부여하였으며, 각 단절 항목별 평균하여 이를 등급화 하였다. 그 결과, F1-G2 구간에서 2등급 이상의 연결성 및 기능성 단절 등급을 보이는 것으로 나타났으며, 다른 구간에서는 연결성 단절이 2등급으로 높지만, 기능성 단절이 3-4등급으로 낮아 복원이 시급한 구간이 아닌 것으로 평가되었다. 본 연구 결과는 향후 수변생태벨트 조성 등의 하천복원 사업에 있어 대상 하천내 우선 사업구간 선정에 기여할 수 있을 것으로 보인다.