• Title/Summary/Keyword: Flow diagnostics

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Measurement of Plasma Parameters (Te and Ne) and Reactive Oxygen Species in Nonthermal Bioplasma Operating at Atmospheric Pressure

  • Choi, Eun Ha;Kim, Yong Hee;Kwon, Gi Chung;Choi, Jin Joo;Cho, Guang Sup;Uhm, Han Sup;Kim, Doyoung;Han, Yong Gyu;Suanpoot, Pradoong
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.02a
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    • pp.141-141
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    • 2013
  • We have generated the needle-typed nonthermal plasma jet by using an Ar gas flow at atmospheric pressure. Diagnostics of electron temperature anddensity is critical factors in optimization of the atmospheric plasma jet source in accordance with the gas flow rate. We have investigated the electron temperature and density of plasma jet by selecting the four metastable Ar emission lines based on the atmospheric collisional radiative model and radial profile characteristics of current density, respectively. The averaged electron temperature and electron density for this plasma jet are found to be ~1.6 eV and ~$3.2{\times}10^{12}cm^{-3}$, respectively, in this experiment. The densities of OH radical species inside the various bio-solutions are found to be higher by about 4~9 times than those on the surface when the argon bioplasma jet has been bombarded onto the bio-solution surface. The densities of the OH radicalspecies inside the DI water, DMEM, and PBS are measured to be about $4.3{\times}10^{16}cm^{-3}$, $2.2{\times}10^{16}cm^{-3}$, and $2.1{\times}10^{16}cm^{-3}$, respectively, at 2 mm downstream from the surface under optimized Ar gas flow 250 sccm.

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Development of a Rapid Automated Fluorescent Lateral Flow Immunoassay to Detect Hepatitis B Surface Antigen (HBsAg), Antibody to HBsAg, and Antibody to Hepatitis C

  • Ryu, Ji Hyeong;Kwon, Minsuk;Moon, Joung-Dae;Hwang, Min-Woong;Lee, Jeong-Min;Park, Ki-Hyun;Yun, So Jeong;Bae, Hyun Jin;Choi, Aeran;Lee, Hyeyoung;Jung, Bongsu;Jeong, Juhee;Han, Kyungja;Kim, Yonggoo;Oh, Eun-Jee
    • Annals of Laboratory Medicine
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    • v.38 no.6
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    • pp.578-584
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    • 2018
  • Background: Accurate, rapid, and cost-effective screening tests for hepatitis B virus (HBV) and hepatitis C virus (HCV) infection may be useful in laboratories that cannot afford automated chemiluminescent immunoassays (CLIAs). We evaluated the diagnostic performance of a novel rapid automated fluorescent lateral flow immunoassay (LFIA). Methods: A fluorescent LFIA using a small bench-top fluorescence reader, Automated Fluorescent Immunoassay System (AFIAS; Boditech Med Inc., Chuncheon, Korea), was developed for qualitative detection of hepatitis B surface antigen (HBsAg), antibody to HBsAg (anti-HBs), and antibody to HCV (anti-HCV) within 20 minutes. We compared the diagnostic performance of AFIAS with that of automated CLIAs-Elecsys (Roche Diagnostics GmbH, Penzberg, Germany) and ARCHITECT (Abbott Laboratories, Abbott Park, IL, USA)-using 20 seroconversion panels and 3,500 clinical serum samples. Results: Evaluation with the seroconversion panels demonstrated that AFIAS had adequate sensitivity for HBsAg and anti-HCV detection. From the clinical samples, AFIAS sensitivity and specificity were 99.8% and 99.3% for the HBsAg test, 100.0% and 100.0% for the anti-HBs test, and 98.8% and 99.1% for the anti-HCV test, respectively. Its agreement rates with the Elecsys HBsAg, anti-HBs, and anti-HCV detection assays were 99.4%, 100.0%, and 99.0%, respectively. AFIAS detected all samples with HBsAg genotypes A-F and H and anti-HCV genotypes 1, 1a, 1b, 2a, 2b, 4, and 6. Cross-reactivity with other infections was not observed. Conclusions: The AFIAS HBsAg, anti-HBs, and anti-HCV tests demonstrated diagnostic performance equivalent to current automated CLIAs. AFIAS could be used for a large-scale HBV or HCV screening in low-resource laboratories or low-to middle-income areas.

Development of the Logistics Network Diagnostic Assessments and Monitoring Service to promote Eco-driving Behaviors for Truck Drivers (화물 운전자의 에코 드라이빙을 위한 물류 네트워크 진단평가 모니터링 서비스 개발)

  • Song, Byung Jun;Ahn, Hyejeong
    • Journal of the Korea Convergence Society
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    • v.2 no.4
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    • pp.15-19
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    • 2011
  • The transportation and logistics industries, the constructions of a green transport methods for logistics systems are presented. They aims the greenhouse gas emissions 37% savings compared to BAU(Business As Usual) for a green growth of national strategy by 2020. An eco-driving training for truck drivers is a typical way. However there are few studies such as the integrated data collection automatically and the diagnostic Assessments and monitoring methods of logistics network status to show the effects of the eco-driving. To solve this problem, this paper provides previous research on logistics network diagnostics assessments and monitoring service and eco-driving. Then this paper shows developments of the logistics network diagnostic assessments and monitoring service business flow to promote eco-driving behaviors for truck drivers based on a smart phone application. Also, it shows an efficiency through an application results in field test.

The Spray Characteristics and Spray Behavior Characteristic in Exhaust Gas Flow of Urea Solution Injector (Urea 수용액 분사용 인젝터의 분무 특성과 배기관내 분무 거동 특성)

  • Oh, Jung-Mo;Han, Young-Deok;Kim, Ki-Bum;Lee, Ki-Hyung
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.34 no.11
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    • pp.999-1004
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    • 2010
  • Recently, many technologies have been developed in order to satisfy stringent emission regulations. However, in the case of diesel engines, the stringent emission regulations with respect to NOx and PM have not yet been satisfied. A dramatic reduction in the NOx and PM emissions could be achieved by using after-treatment systems such as lean NOx trap (LNT) and urea-SCR systems. However, the high temperature in the exhaust pipe affects the spray behavior of the secondary injector, which is used for supplying the Urea-SCR. Because of this high temperature, it is difficult to achieve uniform distribution of the reducing agent in the manifold. In this paper, the characteristics of a urea-SCR injector used for injecting in the exhaust pipe are presented. The purpose of this study was to investigate the spray characteristics of the injector, such as the spray angle, injection quantity, and SMD. In addition, laser diagnostics and high-speed-camera images were used to analyze the injector spray characteristics and to present a distribution of reduction in the transparent manifold.

Comparative Study on Microwave Probes for Plasma Density Measurement by FDTD Simulations

  • Kim, D.W.;You, S.J.;Na, B.K.;Kim, J.H.;Chang, H.Y.;Oh, W.Y.
    • Proceedings of the Korean Vacuum Society Conference
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    • 2014.02a
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    • pp.218.1-218.1
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    • 2014
  • In order to measure the absolute plasma density, various probes are proposed and investigated and microwave probes are widely used for its advantages (Insensitivity to thin non-conducting material deposited by processing plasmas, High reliability, Simple process for determination of plasma density, no complicate assumptions and so forth). There are representative microwave probes such as the cutoff probe, the hairpin probe, the impedance probe, the absorption probe and the plasma transmission probe. These probes utilize the microwave interactions with the plasma-sheath and inserted structure (probe), but frequency range used by each probe and specific mechanisms for determining the plasma density for each probe are different. In the recent studies, behaviors of each microwave probe with respect to the plasma parameters of the plasma density, the pressure (the collision frequency), and the sheath width is abundant and reasonably investigated, whereas relative diagnostic characteristics of the probes by a comparative study is insufficient in spite of importance for comprehensive applications of the probes. However, experimental comparative study suffers from spatially different plasma characteristics in the same discharge chamber, a low-reproducibility of ignited plasma for an uncertainty in external discharge parameters (the power, the pressure, the flow rate and so forth), impossibility of independently control of the density, the pressure, and the sheath width as well as expensive and complicate experimental setup. In this paper, various microwave probes are simulated by finite-different time-domain simulation and the error between the input plasma density in FDTD simulations and the measured that by the unique microwave spectrums of each probe is obtained under possible conditions of plasma density, pressure, and sheath width for general low-temperature plasmas. This result shows that the each probe has an optimum applicable plasma condition and reliability of plasma density measurement using the microwave probes can be improved by the complementary use of each probe.

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Experimental Study of Operating Parameters for Pneumatic Control Valve in Abnormal Conditions (공기구동 제어밸브 비정상상태 운전변수에 관한 실험적 연구)

  • Kim, Yang-seok;Kim, Dae-woong;Lee, Byoung-oh;Jeoung, Rae-hyuk;Lee, Seung-ho
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.40 no.6
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    • pp.613-619
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    • 2016
  • A pneumatic control valve performs a major role in controlling the flow of a system or the level of a key tank in many power plants, and its performance should be guaranteed during the plant's lifetime. Its operation starts by supplying air to the pneumatic actuator or by exhausting the air from the actuator. To control the valve position, the amount of air supply or exhaust is adjusted by a control loop where various accessaries are equipped. In this paper, air leakage in the air supply line, changes in the valve packing force, and false adjustments of zero and the span of the positioner are simulated and analyzed using a 2-in pneumatic valve with a position control loop including an I/P converter and positioner, where the valve position is controlled within ${\pm}2%$ of the control pressure at 67% opening position.

A Study on the Characteristic and Droplet Uniformity of Spray Injection to Exhaust Gas Flow from Urea Solution Injector (Urea 수용액의 배기가스 유동장내 분무 특성과 분무 균일도에 관한 연구)

  • Oh, Jung-Mo;Cha, Won-Sim;Kim, Ki-Bum;Lee, Jin-Ha;Lee, Ki-Hyung
    • Transactions of the Korean Society of Automotive Engineers
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    • v.19 no.3
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    • pp.83-89
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    • 2011
  • Diesel engines can produce higher fuel efficiency and lower $CO_2$ emission, they are subject to ever more stringent emission regulation. However, there are two major emission concerns fo diesel engines like such as particulate matter (PM) and nitrogen oxides (NOx). Moreover, it is not easy to satisfy the regulations on the emission of NOx and PM, which are getting more strengthened. One of the solutions is to apply the new combustion concept using multistage injection such as HCCI and PCCI. The other solution is to apply after-treatment systems. For example, lean NOx trap catalyst, Urea-SCR and others have various advantages and disadvantages Especially, Urea-SCR system have advantages such as a high conversion efficiency and a wide operation conditions. Hence the key factor to implementation of Urea-SCR technology, good mixing of urea(Ammonia) and gas, reducing Ammonia slip. Urea mixer components are required to facilitate evaporation and mixing because the liquid state of urea poses significant barriers for evaporation, and the distance to mixer is the most critical that affect mixer performance. In this study, to find out the distance from injector to mixer and simulation factor, a laser diagnostics and high speed camera are used to analyze urea injector spray characteristics and to present a distribution of urea solution in transparent manifold In addition, Droplet Uniformity Index is calculated from the acquired images by using image processing method to clarify the distribution of spray.

A Study of Development of Diagnostic System for Web Application Vulnerabilities focused on Injection Flaws (Injection Flaws를 중심으로 한 웹 애플리케이션 취약점 진단시스템 개발)

  • Kim, Jeom-Goo;Noh, Si-Choon;Lee, Do-Hyeon
    • Convergence Security Journal
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    • v.12 no.3
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    • pp.99-106
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    • 2012
  • Today, the typical web hacking attacks are cross-site scripting(XSS) attacks, injection vulnerabilities, malicious file execution and insecure direct object reference included. Web hacking security systems, access control solutions, access only to the web service and flow inside but do not control the packet. So you have been illegally modified to pass the packet even if the packet is considered as a unnormal packet. The defense system is to fail to appropriate controls. Therefore, in order to ensure a successful web services diagnostic system development is necessary. Web application diagnostic system is real and urgent need and alternative. The diagnostic system development process mu st be carried out step of established diagnostic systems, diagnostic scoping web system vulnerabilities, web application, analysis, security vulnerability assessment and selecting items. And diagnostic system as required by the web system environment using tools, programming languages, interfaces, parameters must be set.

A Study on Temporal Measurement of Size and Concentration for Soot Aggregates among Exhaust Emissions in Diesel Engines (디젤기관 배기 배출물에 있어서 매연 응집체 크기 및 농도의 시간적 계측에 관한 연구)

  • Bae, Myung-Whan;Kamimoto, Takeyuki;Bae, Chang-Hwan
    • Transactions of the Korean Society of Automotive Engineers
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    • v.15 no.1
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    • pp.116-124
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    • 2007
  • An optical method to measure the size and number density of soot aggregates in diesel exhaust has been proposed in this study. Two laser beams in co-axial alignment transmit a soot loaded exhaust gas flow, and the transmittance at each wavelength is detected by a photo diode simultaneously. The volume equivalent diameter and number density of soot aggregates in the optical path can be theoretically given by the transmittance values measured at two wavelengths. A test conducted by a single cylinder, 4 cycle, small and DI diesel engine shows that the temporal variations of the size and number density of soot aggregates in the diesel exhaust can be measured by the proposed method at a transient mode operation. It is found that the volume equivalent diameter varied temporally from 70 to 110 nm during the period that high soot concentration is observed. One can also conclude that the optical length longer than 1 m in the dynamic range regarding this method is preferable for measuring soot concentration at the level of $1\;mg/m^3$.

A Simple, Rapid, and Automatic Centrifugal Microfluidic System for Influenza A H1N1 Viral RNA Purification

  • Park, Byung Hyun;Jung, Jae Hwan;Oh, Seung Jun;Seo, Tae Seok
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.277.1-277.1
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    • 2013
  • Molecular diagnostics consists of three processes, which are a sample pretreatment, a nucleic acid amplification, and an amplicon detection. Among three components, sample pretreatment is an important process in that it can increase the limit of detection by purifying nucleic acid in biological sample from contaminants that may interfere with the downstream genetic analysis such as nucleic acid amplification and detection. To achieve point-of-care virus detection system, the sample pretreatment process needs to be simple, rapid, and automatic. However, the commercial RNA extraction kits such as Rneasy (Qiagen) or MagnaPure (Roche) kit are highly labor-intensive and time-consuming due to numerous manual steps, and so it is not adequate for the on-site sample preparation. Herein, we have developed a rotary microfluidic system to extract and purify the RNA without necessity of external mechanical syringe pumps to allow flow control using microfluidic technology. We designed three reservoirs for sample, washing buffer, and elution buffer which were connected with different dimensional microfluidic channels. By controlling RPM, we could dispense a RNA sample solution, a washing buffer, and an elution buffer successively, so that the RNA was captured in the sol-gel solid phase, purified, and eluted in the downstream. Such a novel rotary sample preparation system eliminates some complicated hardwares and human intervention providing the opportunity to construct a fully integrated genetic analysis microsystem.

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