• Journal of Life Science
• /
• v.20 no.12
• /
• pp.1851-1858
• /
• 2010

Estrogen deficiency in peri- and postmenopausal women results in variety of neurovegetative, psychic and somatic symptoms, and may contribute to severe diseases within the aged female population. Ecklonia stolonifera (ES) is an edible brown algae traditionally used in fishery towns in Far East Asia. This study was performed to investigate the effects of ES extracts on blood flow and serum lipid concentration in ovariectomized rats. Weight-matched female Sprague-Dawley strain rats were assigned to four groups. Three groups were surgically ovariectomized (OVX). The fourth group was sham operated. Rats were randomly assigned to the following groups: sham-operated rats (Sham), ovariectomized control rats (OVX-CON), ovariectomized rats supplemented with ES extract at 50 mg/kg bw/day (OVX-ES50) and ovariectomized rats supplemented with ES extract at 200 mg/kg bw/day (OVX-ES200). Serum total cholesterol and triglyceride contents decreased in the sham group compared to the OVX-CON group. Six weeks feeding of ES extract resulted in a significant (p<0.05) lowering of serum triglyceride and a lowering tendency of total cholesterol level. The level of Hdl-cholesterol in serum increased by supplementation of ES extracts at 50 mg/kg and 200 mg/kg bw/day (p<0.05). Blood passage time of the ES extracts-supplemented group was higher than the OVX-CON group. The ability of platelet aggregation of groups treated with ES extracts was less than that of the OVX-CON group. These results suggest that the beneficial effects of ES extract may be used to possibly improve on the metabolic syndrome of menopausal women.

• Journal of Korean Society of Coastal and Ocean Engineers
• /
• v.20 no.2
• /
• pp.203-211
• /
• 2008

More recently, the massive marine bridges in a ship passage have been constructed on the sea. Therefore, the ship impact protection for the bridge-piers are installed to consider the possibility of vessel collision danger. Due to the ship impact protection, the pier-scour characteristics are changed in comparison with the condition without the ship impact protection (SIP). In this study, the physical modeling for the Incheon Sea-Crossing Bridge was performed to analyze the pier-scour characteristics with respect to the vessel collision protection. The rigid and movable bed tests were conducted to evaluate the flow pattern, scour depth, and scourhole with and without the ship impact protection. The experimental results for the maximum scour depth is increased 0.24 m in W1 pier at the same location and 2.4 m in W2+3+4 piers due to the SIP installation. Especially, the maximum scour depth in W2+3+4 piers was occurred around the SIP.

• Journal of Animal Science and Technology
• /
• v.56 no.4
• /
• pp.14.1-14.7
• /
• 2014

Two trials were conducted to evaluate the influence of supplemental urea withdrawal on characteristics of digestion (Trial 1) and growth performance (Trial 2) of feedlot cattle during the last 40 days on feed. Treatments consisted of a steam-flaked corn-based finishing diet supplemented with urea to provide urea fermentation potential (UFP) of 0, 0.6, and 1.2%. In Trial 1, six Holstein steers ($160{\pm}10kg$) with cannulas in the rumen and proximal duodenum were used in a replicated $3{\times}3$ Latin square experiment. Decreasing supplemental urea decreased (linear effect, $P{\leq}0.05$) ruminal OM digestion. This effect was mediated by decreases (linear effect, $P{\leq}0.05$) in ruminal digestibility of NDF and N. Passage of non-ammonia and microbial N (MN) to the small intestine decreased (linear effect, P = 0.04) with decreasing dietary urea level. Total tract digestion of OM (linear effect, P = 0.06), NDF (linear effect, P = 0.07), N (linear effect, P = 0.04) and dietary DE (linear effect, P = 0.05) decreased with decreasing urea level. Treatment effects on total tract starch digestion, although numerically small, likewise tended (linear effect, P = 0.11) to decrease with decreasing urea level. Decreased fiber digestion accounted for 51% of the variation in OM digestion. Ruminal pH was not affected by treatments averaging 5.82. Decreasing urea level decreased (linear effect, $P{\leq}0.05$) ruminal N-NH and blood urea nitrogen. In Trial 2, 90 crossbred steers ($468kg{\pm}8$), were used in a 40 d feeding trial (5 steers/pen, 6 pens/treatment) to evaluate treatment effects on final-phase growth performance. Decreasing urea level did not affect DMI, but decreased (linear effect, $P{\leq}0.03$) ADG, gain efficiency, and dietary NE. It is concluded that in addition to effects on metabolizable amino acid flow to the small intestine, depriving cattle of otherwise ruminally degradable N (RDP) during the late finishing phase may negatively impact site and extent of digestion of OM, depressing ADG, gain efficiency, and dietary NE.

• Korean Journal of Ichthyology
• /
• v.21 no.4
• /
• pp.262-272
• /
• 2009

Upstream behavior of glass eels was examined in an experimental eel-ladder at a laboratory of the National Fisheries Research and Development Institute from March to May in 2008. The study was made under various environmental factors and conditions affecting the upstream migration of glass eels in order to design a functional ladder that would allow the passage of glass eels. The experimental eel-ladder consisted of an upper freshwater chamber and a lower sea water chamber; glass eels in sea water can move up to the upper freshwater chamber through the slope (eel-ladder) between them. The optimal condition of the eel-ladder was estimated by comparing the number of glass eels that moved upstream depending on various conditions. Since the glass eels actively moved up the slope to river water rather than to reservoir water or tap water, the experiment was realized using river water. A significantly higher number of glass eels moved up during the spring tide than during the neap tide, and during night than during the day. Upstream movement was significantly higher during high tide than during low tide. Glass eels effectively moved up through a slope of less than $30^{\circ}$ and water-flow velocity lower than 0.4 m/sec. The fish preferred a coarser ladder bed covered with small gravels, brush or carpet.

• Journal of Embryo Transfer
• /
• v.31 no.3
• /
• pp.161-168
• /
• 2016

Nucleotide metabolism in endothelium is variable between different species. Recent studies demonstrated that this variability could contribute coagulation dysfunction, even though organs of the alpha 1,3-galactosyltransferase gene knockout pig were transplanted into the primate. CD73 (ecto-5'-nucelotidase) is an enzyme at cell surface catalyzing the hydrolysis of adenosine triphosphate to adenosine, which plays role on a substance for anti-inflammatory and anti-coagulant. Thus, overexpression of CD73 in endothelial cells of the pig is considered as an approach to reduce coagulopathy. In this study, we constructed a human CD73 expression vector under control of porcine Icam2 promoter (pIcam2-hCD73), which is expressed specifically at endothelial cells, and of CMV promoter as a control (CMV-CD73). First, we transfected the CMV-CD73 vector into HEK293 cells, and then confirmed CD73 expression at cell surface by flow cytometry analysis. Next, we transfected the pIcma2-CD73 and CMV-CD73 vectors into primary porcine fibroblasts and endothelial cells. Consequence was that the pIcma2-CD73 vector was expressed only at the porcine endothelial cells, meaning that the pIcam2 promoter lead to endothelial cell-specific expression of CD73 in vitro. Finally, we nucleofected the pIcam2-hCD73 vector into passage 3 fibroblasts, and enforced hygromycin selection of 400mg/ml. We were able to obtain forty three colonies harboring pIcam2-CD73 to provide donor cells for transgenic cloned porcine production.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.42 no.1
• /
• pp.148-152
• /
• 2013

An ultraviolet (UV) sterilization system was developed to decrease the number of microorganisms in filtered Acer mono sap (FAS). The Acer mono sap (AS) was passed through 18 strips of PTA fluoroplastic tubing with 30 UV lamps (total 1,170 W). During passage, the AS was effectively exposed to the UV rays. The total bacteria, coliform group and fungi were sterilized at a flow rate ranging from 852 to 1,358 mL/min and a UV power higher than 156 W. Although the sensory score of the UV-sterilized AS was significantly lower than that of the untreated AS, the sensory score was better under the condition of 390 W and 852~1,358 mL/min than under the other conditions examined. ANOVA did not reveal a significant difference in pH, total acidity, sugar content and color characteristic under all conditions tested (p<0.05). Considering the overall attributes of the AS quality, the optimum sterilization condition was determined to be 390 W and 852~1,358 mL/min.

• Journal of Advanced Marine Engineering and Technology
• /
• v.40 no.1
• /
• pp.69-74
• /
• 2016

Bathymetric changes were studied in the southern sea off the Jinwoo-do Island, which is one of the deltaic barrier islands surrounding the Nakddong river estuary. In this study, 16 bathymetry data sets were obtained from June 2006 to April 2015. Two narrow channels, the one lying between Jinwoo-do and Shinja-do, and the other one lying between Nulcha-do and Jinwoo-do extended into the eastern and western parts of the study area, respectively. The eastern extension of the channel contained a passage of mixed estuarine waters of seawater and river water discharged from the Nakdong river barrier and the west Nakdong River. The western channel connected the Nakdong River estuary with the Busan New Port via a connecting pier. Total volumetric changes of sediments in study area and discharge flow of the Nakdong river barrier were analyzed. Bottom topographical changes occurred mainly in the eastern extension of the channel. These changes were initially characterized by gradual erosion or deposition followed by rapid restoration. The total volume of sediment gradually increased from June 2006 to March 2013, but experienced a sudden decrease in October 2013 because of typhoon Danas. Few fluctuations were observed from October 2013 to April 2015. Analysis of the cross-sectional bathymetry of the north-south direction showed that the deepest point of the eastern channel moved 100-130 m westward and 200 m northward between June 2006 and April 2015.

• Korean Journal of Microbiology
• /
• v.49 no.3
• /
• pp.275-281
• /
• 2013

Probiotics have been reported to benefit human health by modulating immunity, lowering cholesterol, improving lactose tolerance, and preventing some cancer. Once ingested, probiotic microorganisms have to survive harsh conditions such as low pH, protease-rich condition, and bile salts during their passage through the gastro-intestinal (GI) tract colonize and proliferate to exert their probiotic effects. The dual coating technology, by which the bacteria are doubly coated with peptides and polysaccharides in consecutive order, was developed to protect the ingested bacteria from the harsh conditions. The aim of the study was to evaluate the viable stability of a doubly coated blend of four species of Bifidobacterium by comparing its bile/acid resistance and heat viability in vitro with that of the non-coated blend. After challenges with acid, bile salts, heat, and viable cell counts (VVCs) of the dual coated and non-coated blend were determined by cultivation on agar plates or flow cytometric measurement after being stain with the BacLigtht kit$^{TM}$. The results showed that the dual coated blend was much higher resistant to the acidic or bile salt condition than the non-coated blend and heat viability was also higher, indicating that the dual coating can improve the survival of probiotic bacteria during their transit through the GI tract after consumption.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.21 no.2
• /
• pp.179-187
• /
• 2015

The purpose of this study is to identify the aspect that the traffic distribution function changes, according to the direction of the datum line and the horizontal and vertical positions of the datum point applied when it is calculated. Targeting routes at the entrance of Mokpo Harbor, this study tested using AIS survey data of January 2013 the effects of the three variables-direction of the datum line(${\theta}$), horizontal position($\mathfrak{L}_H$) and vertical position($\mathfrak{L}_V$) on mean ($\bar{x}$) and standard deviation (${\delta}$). The test result showed that $\bar{x}$ and ${\delta}$ were changed according to the change of ${\theta}$, because the extracted sample data were changed according to ${\theta}$; and the changes of $\bar{x}$ and ${\delta}$ according to ${\theta}$ were drawn as the relation of the sine function' sum. In addition, it was found that setting up ${\theta}$ that the change value of ${\delta}$ becomes the least as the direction of the datum line was valid, to determine the optimum passage distribution function on complex waters with multiple branches of route. The result of this study is expected to be used as basic data to understand maritime traffic flow based on more quantified data of normal distribution and make decisions related to maritime traffic safety management.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.18 no.2
• /
• pp.433-442
• /
• 2017

This study consists of five case studies of the catering facilities of newly-built elementary schools' in Sejong City, regarding the spatial configuration and allocation. The analysis indicated that the space allocated in the cooking area in the five schools compared to the standard was as follows: School A; 99.2%, B; 91.1%, C; 81.4%, D; 110.8%, E; 88.1%. Accordingly, the cooking area was close to, or slightly above the standard; nevertheless, other areas in the facilities were not allocated sufficient space and were not appropriately planned. Among the other findings, the receiving areas of three schools were not even planned, and the receiving works were carried out in the pre-preparation area. Considering the flow of the dish-washing process, the width of the room should be at least 3.6 meters with a passage width of 1.6 meters, and easy access to the cooking and dining areas must be equipped. An interview with all five supervisors showed that most complaints focused on the cooking and dishwashing areas, whereas there were none in the storage and workers' area. The result highlights the importance of the cooking and dishwashing areas as the main functions in catering facilities, and future planning strategies should consider this aspect.