• Journal of fish pathology
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• v.37 no.1
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• pp.123-132
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• 2024

Viral diseases cause enormous economic losses to the olive flounder (Paralichthys olivaceus) aquaculture industry in Korea. This study aimed to identify immune-related genes expressed in the kidney of olive flounder injected with Polyinosinic-polycytidylic acid (Poly (I:C)). Thirty fish were divided into two groups by intraperitoneal injection of 100µl of diethylpyrocarbonate-treated water or poly I:C per fish. Kidney tissues at day 3 and 30 after the injection were used for RNA-seq analysis to identify differentially expressed genes (DEGs). Poly I:C group upregulated il8, cfh, tnfaip2b, c3b.2, ly6d and cd38 genes at 3 days post-injection. Additionally, cd22, ccl34a.3, c9, cxcl19, ccl27a, ccl7, and cfh genes were upregulated at 30 days post-injection. Differential expression gene analysis showed that poly I:C has both short and long-term immune effects in olive flounder. This study provides a theoretical basis for understanding the molecular mechanism of the short and long-term immune effects of poly I:C.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.47 no.6
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• pp.869-873
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• 2014

Cardiorespiratory variables were measured in flounder Paralicthys olivaceus exposed to acidified seawater equilibrated with a gas mixture containing 5% $CO_2$ gas for 72 h at $20^{\circ}C$. It was found that $CO_2$ produced a significant increase in arterial $PCO_2$ ($PaCO_2$) and significant decreases in arterial pH (pHa). $CO_2$ transiently increased heart rate within 30 min of exposure. After cardiac output was elevated by the increase in heart rate within 30 min, was then reduced by the decrease in both stroke volume and heart rate. The heart responses of flounder differ from those of yellowtail to high $CO_2$ environment.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.6
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• pp.785-790
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• 2011

This study investigated the nutritive quality of olive flounder Paralichthys olivaceus fed either moist pellet (MP) or moist pellet mixed with mushroom extract (MPME) for 6 months. There was no significant difference in crude protein or extractive nitrogen in the muscle of flounder fed MP versus MPME (P > 0.05). The total amino acid content in the muscle of flounder fed MP was $15.22{\pm}5.24$ g/100 g, compared to $19.90{\pm}2.90$ g/100 g for flounder fed MPME. Essential amino acid content was $7.04{\pm}2.21$ g/100 g in the muscle of flounder fed MP versus $8.94{\pm}2.50$ g/100 g for MPME. Total amino acid content was higher in the muscle of olive flounder fed MPME, while essential amino acid content was higher in flounder fed MP. The ratio of non-essential amino acids to essential amino acids was $0.86{\pm}0.07$ for flounder fed MP and $0.81{\pm}0.08$ for flounder fed MPME. There was no significant difference in free amino acid content and fatty acid composition. The breaking strength of muscle of olive flounder fed MP was higher ($1.44{\pm}0.51\;kg/cm^2$) than in flounder fed MPME ($1.29{\pm}0.30\;kg/cm^2$). There was no evidence that dietary additives, such as mushroom extract, increase growth rate or nutritive quality of olive flounder.

• Journal of fish pathology
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• v.19 no.2
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• pp.183-188
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• 2006

In fish vaccinology, the secondary antibodies against fish immunoglobulins (Igs) are necessary to measure specific humoral immune responses in immunized fish. In the present study, polyclonal antiserum against olive flounder (Paralichthys olivaceus) IgM heavy chain was generated by intramuscular immunization of rabbit with Escherichia coli/eukaryotic shuttle vector containing open reading frame (ORF) of olive flounder IgM heavy chain. Western blot analysis demonstrated the specific activity of the rabbit antiserum with reduced olive flounder serum H chain at dilutions up to 1:1000. Titer of immunized rabbit serum against olive flounder serum was significantly higher than that of pre-immunized rabbit serum when determined by ELISA.

• The Korean Journal of Food And Nutrition
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• v.12 no.5
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• pp.496-501
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• 1999

Albinism is a phenomenon that color of the body surface is changed to white orfaint brown from the specific color to the species by difficiency of pigments due to mutation or disease. This study was undertaken to investigate the experimental basis on the appearance of albinism in cultured flounder Paralichthys olivaceus. The skin and muscle from the normal and albinic flounder were used by measuring contents of amino acid free amino acid. The results were summarized as follows: Contents of amino acid in theskin and muscle are different from normal and albinic flounder. Phenolic and sulfuric amino acids in the skin of normal flounder were 2 times those of albinic ones. Especially tyrosine contents of the skin in normal flounder were 24 times higher than those. Methionine was 26 times higher than those Phenylalanine was 1.6 times higher. In free amino aicd phosphoserine and phosphoethanolamine were a little higher than that ones. The melanin formation of the skin in flounder was affected by substrates such as phenolic amino acid and cofactor such as sulfuric amino acid.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.5
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• pp.696-703
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• 2015

We investigated the characteristics of embryonic and abnormal organ development in haploid olive flounder, Paralichthys olivaceus, by comparing egg development and histological sections in haploid and diploid individuals. After the mid-blastula transition, abnormal development was observed in haploid fish, including delayed epiboly and malformation of the germ ring and embryonic body. In haploid flounder, Kupffer’s vesicles are irregularly shaped and of variable size compared to diploids. The embryonic body of haploids was shorter and broader than that of diploids and the tail length and size were variable. Most haploid embryos failed to hatch and the few larvae that did, did not survive for more than 6 h. The histological analysis of haploid larvae revealed deformed development in diverse organs, including the eye, otic vesicles, notochord, and neural tube. These results may be related to an abnormality in the axial system of haploid larvae. This study confirmed that the abnormalities of haploid olive flounder were similar to the reported characteristics of haploid syndrome. The abnormalities are caused by delayed epiboly and involution and deformity of Kupffer’s vesicle during egg development.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.3
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• pp.464-470
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• 2020

This study was conducted to replace fish meal (FM) with three plant proteins (soybean meal, soy protein concentrate, and wheat gluten) in diets for growing olive flounder Paralichthys olivaceus. The control diet was formulated to contain 65% sardine FM and four other replacement diets were formulated to replace FM with the plant proteins by 25, 30, 35 and 40% (designated FM25, FM30, FM35 and FM40, respectively). The replacement diets were added with three essential amino acids (lysine, methionine and threonine) to meet their requirements for the fish. Olive flounder (initial average weight, 96.8±0.2 g) were randomly distributed into 20 tanks (425 L each) at a density of 25 fish per tank. Four replicate groups of fish were fed one of the diets two times daily for 15 weeks. At the end of the feeding trial, no significant differences were found among all the fish groups in growth performance, feed utilization, nonspecific immune responses and hematological health parameters. Thus, this result indicates that the plant proteins with the three limiting amino acids could replace FM up to 40% in diets for growing olive flounder.

• Journal of Aquaculture
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• v.14 no.1
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• pp.57-64
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• 2001

In Paralichthys olivaceus and Nibea japonica, response to live air (5hr) or ship (25hr) transportation was assessed, by determining the levels of plasma cortisol, glucose, lactic acid and osmolality, as well as hematological parameters namely hematocrit (Ht), red blood cell (RBC) count, hemoglobin (Hb), corpuscular volume (MCV), corpuscular hemoglobin (MCH) and corpuscular hemoglobin concentration (MCHC). In the experimental series I, the olive flounder was subjected to stress or no stress for 1hr, prior to its air transportation for 5hr. The stress suffered by the flounder prior to air transportation resulted in significant reduction in Ht, but increases in MCH and MCHC. Air transportation led to increases in MCV and MCH in both the stressed and non stressed groups. In the non stressed group it led to significant increase in Ht but decrease in MCHC. In the stressed group, the air transportation led to significant increases in osmolality and plasma cortisol from 5 to 38.5ng/$m\ell$. Non stressed groups did not show significant differences in this before and after transportation. In the experiment II, the red blood cell (RBC) count ranged from 2.5$\times$10$^{6}$ /${mu}ell$ to 2.7$\times$10$^{6}$ /${mu}ell$ in the flounder and 1.9$\times$10$^{6}$ /${mu}ell$ to 2.1$\times$10$^{6}$ /${mu}ell$ in the croaker during the pre- and post-transportation, respectively. In the croaker the shipping led to significant increase in plasma cortisol from 26 to 35ng/$m\ell$ but decrease in glucose from 91.0 to 26.4mg/㎗. For glucose the reverse (39.0 to 51.0mg/㎗) was true for the flounder.

• Journal of Aquaculture
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• v.12 no.3
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• pp.221-228
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• 1999

The effects of sparp changes of water temperature (WT) on the stree response and physiological change of the cultured olive flounder (Paralichthys olivaceus) were examined by manipulating WT (3 patterns) in a running seawater culture system. In the first group (Exp. I), the WT was decreased from 18$^{\circ}C$ to 11$^{\circ}C$ within 6 hours and increased back to the original WT quickly. WT was decreased from 2$0^{\circ}C$ to 11$^{\circ}C$ within 5 hours and main-tained at 11$^{\circ}C$ for 10 hours. and then increased to 2$0^{\circ}C$ in the second group (Exp. II). In the third group(Exp. III) WT was decreased to 11$^{\circ}C$ within 5 hours (type A) or 10 hrs. (type B). In Exp. I and III, the level of serum cortisol was increased from 2.5$\pm$0.3 ng/ml and 2.6$\pm$0.9 ng/ml to 13.6$\pm$3.0 ng/ml and 12.4$\pm$3.2ng/ml, respectively, with WT decrease. However, no consistent tendency in the change of serum glucose level was shown according to WT decrease. In Exp. III, the glucose level of fish in type A was decreased until 5 hours and increased at 7 hours, then decreased until 12 hours where as the glucose level in type B was decreased until 5 hours and stayed at the level of 15.7 mg/dl. The serum osmolality was reduced with WT decrease and the response of serum electrolytes in this experiment conflicted, and a tendency in total protein, AST and ALT was not found following WT decrease. In conclusion, olive flounder responded to the stress caused by WT decrease and acclimated to this condition when the lower temperature was maintained. But there was no stress response in the blood of olive flounder when WT was increased. On the other hand, the degree of stress response in olive flounder was various according to the range and gradient of WT change.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.11
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• pp.682-687
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• 2018

This study investigated the food value of Paracyclopina nana for flounder Paralichthys olivaceus larvae in two feeding stages, rotifer (for 12 days) and Artemia (for 16 days). In the rotifer feeding stage, survival and growth of flounder larvae in the only P. nana (nauplii) feeding experiment were higher than in the only rotifer feeding experiment on 12 DAH (days after hatching). In the Artemia feeding stage, the growth of flounder larvae in the only P. nana (C4-adult) feeding experiment and mixture feeding experiment (P. nana+Artemia) were higher than in other experiments on 30 DAH, but the survival of flounder larvae did not differ significantly among experiments. The n-3 HUFA contents of nauplius and C4-adult were 4.0% and 5.4%, respectively. Overall the results of this study indicate that the brackish water cyclopoid copepod, P. nana, is an effective live food organism for larval seedling production of marine fish.