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Generation of polyclonal antiserum to olive flounder (Paralichthys olivaceus) immunoglobulin by immunization of rabbit with plasmids containing heavy chain gene of olive flounder immunoglobulin  

Kim, Gi-Hong (부경대학교)
Kwon, Se-Ryun (부경대학교)
Kim, Chun-Soo (부경대학교)
Lee, Eun-Hye (부경대학교)
Publication Information
Journal of fish pathology / v.19, no.2, 2006 , pp. 183-188 More about this Journal
Abstract
In fish vaccinology, the secondary antibodies against fish immunoglobulins (Igs) are necessary to measure specific humoral immune responses in immunized fish. In the present study, polyclonal antiserum against olive flounder (Paralichthys olivaceus) IgM heavy chain was generated by intramuscular immunization of rabbit with Escherichia coli/eukaryotic shuttle vector containing open reading frame (ORF) of olive flounder IgM heavy chain. Western blot analysis demonstrated the specific activity of the rabbit antiserum with reduced olive flounder serum H chain at dilutions up to 1:1000. Titer of immunized rabbit serum against olive flounder serum was significantly higher than that of pre-immunized rabbit serum when determined by ELISA.
Keywords
Genetic immunization; Polyclonal antiserum; Olive flounder; Immunoglobulin INTRODUCTIONIn fish vaccinology;
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