• 한국어병학회지
• /
• 제17권3호
• /
• pp.217-222
• /
• 2004

This study was performed to investigate the immunological side effects of chloramphenicol (CAP) on olive flounder, Paralichthys olivaceus. To investigate immunological effects on olive flounder, we determined the changes of chemiluminescence (CL) response of flounder kidney-derived leucocyte after the treatment of CAP in vivo and in vitro. The CL activity was significantly decreased in a dose-dependent manner during the treatment of CAP in vitro. Similarly, a dose-dependent reduction of CL response, although not significant, were observed during the treatment of CAP in vivo. The results suggest that CAP reduced the function of flounder phagocytosis in vivo and in vitro, indicating the immunosuppressive ability of CAP.

• 한국수산과학회지
• /
• 제36권5호
• /
• pp.442-448
• /
• 2003

Melanin concentrating hormone (MCH) regulating color change of fish skin was identified from brain cDNA library of Olive flounder (Paralichthys olivaceus) during the analysis of Expressed Sequence Tags (ESTs). Olive flounder MCH gene consisted of 598 nucleotides encoding 150 amino acids. Olive flounder MCH protein revealed to contain signal peptide of 19 amino acid residues, pro-MCH of 131 amino acids being processed to biologically active and mature form of hormone with 25 amino acid residues at the carboxyl terminus. A comparative structural analysis revealed that Olive flounder MCH precursor had low sequence identity with other fish species and mammalian counterparts, while the amino acid sequences of mature hormone had a relatively high identity and more conserved. RT-PCR analysis revealed that olive flounder MCH precersor gene was expressed spectically only in the brain and not in other tissues.

• 수산해양기술연구
• /
• 제50권4호
• /
• pp.583-594
• /
• 2014

This study was performed to estimate biomass and provide management guidance through population ecological characteristics, including growth parameters, instantaneous coefficients of natural and fishing mortalities, and age at first capture of the starry flounder, Platichthys stellatus and olive flounder, Paralichthys olivaceus of Korea. For describing growth of this species, a von Bertalanffy growth model was adopted. The von Bertalanffy growth parameters estimated from a non-linear regression for starry flounder were $L_{{\infty}}=48.25cm$, K=0.16/yr, and $t_0=-1.48$, respectively and those for olive flounder were $L_{{\infty}}=86.46cm$, K=0.26/yr, and $t_0=-0.29$, respectively. Biomass of Platichthys stellatus was estimated by direct biomass estimation method was 2.6 M/T, that was estimated by indirect method was 13.4 M/Tt. Those of Paralichthys olivaceus were estimated as 10.1 M/T, 19.3 M/T, respectively. An yield per recruit analysis showed that the current yield per recruit on Platichthys stellatus was about 48.2 g with F=0.646/yr and the age at first capture ($t_c$) 1.35yr, that on Paralichthys olivaceus was about 167.6 g with F=1.121/yr and the age at first capture ($t_c$) 1yr.

• 한국수산과학회지
• /
• 제39권3호
• /
• pp.303-307
• /
• 2006

The effects of starvation on histological changes in hepatocytes and the intestinal epithelium in the olive flounder, Paralichthys olivaceus were examined. The starved group was not fed for 12 weeks, whereas the fed group was given food. Food deprivation resulted in a significant decrease in hepatocyte nucleus size and nuclear height in the intestinal epithelium (P<0.05). In the starved group, pronounced degenerative changes were seen in the histology of hepatocytes and the intestinal epithelium; the tissue morphology was irregular and the nuclei were stained basophillic, shrunk irregularly, and very compact. These results suggest that histological changes in hepatocytes and the intestinal epithelium can be used as an indicator of starvation in the olive flounder. In addition, the data are interpreted in detail and some biologically important relationships are discussed.

• Fisheries and Aquatic Sciences
• /
• 제4권1호
• /
• pp.25-31
• /
• 2001

4-Aminobutyrate aminotransferase plays an essential role in the 4-aminobutyric acid shunt, converting 4-aminobutyrate to succinic semi aldehyde. We isolated and sequenced' a fish cDNA fragment that encodes 4-aminobutyrate aminotransferase. A brain cDNA library from flounder (Paralichthys olivaceus) was constructed using the ZAP- III XR vector and screened for the fish 4-aminobutyrate aminotransferase gene using a probe derived from the conserved sequences of known mammalian 4-aminobutyrate aminotransferases. A partial cDNA for 4-aminobutyrate aminotransferase was cloned and found to be 700 bp in length corresponding to 66 amino acids. Nucleotide sequence of the clone was aligned with NCBI (National Center for Biotechnology Information) DNA sequence data base. The result showed high sequence identity with previously reported mammalian 4-aminobutyrate aminotransferases. The trans­criptional level of flounder 4-aminobutyrate aminotransferase was detected with the presence of mRNA at different flounder tissues by reverse transcription-polymerase chain reaction (RT-PCR). The expression of flounder 4-aminobutyrate aminotransferase was also tested and detected from the flounder tissues of the brain, liver, kidney and pancreas.

• 한국수산과학회지
• /
• 제47권5호
• /
• pp.611-621
• /
• 2014

A nationwide survey was conducted to study the rate of Kudoa septempunctata parasitization in cultured olive flounder, Paralichthys olivaceus, in Korea in 2013. Of 1107 olive flounder sampled randomly from 89 culture farms in five different regions in Korea, K. septempunctata was detected only in Jeju, where 10 of 318 fish (3.14%) were PCR positive, with genomic concentrations of $4.67{\times}10^5$ to $1.48{\times}10^{11}$ rDNA copies/g by real-time PCR. Of the ten Kudoapositive fish, K. septempunctata spores with 5-7 polar capsules were detected only in four fish. No samples of K. septempunctata were detected in olive flounder from the other regions surveyed. Furthermore, K. septempunctata was not detected in 326 samples of olive flounder seeds sampled from 39 hatcheries in seven different regions in Korea. Therefore, the parasite infection is restricted to Jeju and K. septempunctata infection is not spread from hatcheries.

• 한국어병학회지
• /
• 제35권2호
• /
• pp.177-183
• /
• 2022

We described Trichodina hokkaidoensis Mizuno, Matsuda, Nishikawa and Ito, 2022 from olive flounder Paralichthys olivaceus by morphological observation and molecular analysis. Morphological parameters of our specimen from 4 different sampling sites (Gangneung, Jeju, Wando, Taean) mostly coincided with those of T. hokkaidoensis. Some morphometric parameters of this trichodinid showed some inconsistency, depending on the sampling locations, but all of their partial small subunit ribosomal DNA sequences (1,182 bp) showed 100% homology with T. hokkaidoensis, originally described from artificially reared juvenile barfin flounder Verasper moseri from Japan. T. hokkaidoensis is known to cause epidermal damages to the host fish. However, there was no considerable pathological lesions in the olive flounder harboring T. hokkaidoensis in this study. The pathogenicity of T. hokkaidoensis against olive flounder needs to be investigated.

• 환경생물
• /
• 제21권4호
• /
• pp.352-357
• /
• 2003

Phenanthrene, one of Polycyclic aromatic hydrocarbons with three aromatic rings, is a ubiquitous contaminant in the environment. Phenanthrene has been identified in ambient air, drinking water and sediment. We examined the effect of phenanthrene on steroid hormones level of olive flounder, Paralichthys olivaceus. Plasma testosterone level was increased significantly in fish exposed to phenanthrene($\geq1.0 \mu M$) at 4th week. However, there was no significant changes of estradiol-17$\beta$ concentration in fish exposed to phenanthrene. The physiological variation in phenanthrene exposed fish was a dramatic increase in plasma cortisol level. It is concluded that chronic exposure of phenanthrene can induce increase of plasma testosterone levels and elevate the plasma cortisol level in flounder, Paralichthys olivaceus.

• 한국수산과학회지
• /
• 제49권5호
• /
• pp.671-674
• /
• 2016

We cytogenetically analyzed a triploid King-Nupchi strain of the olive flounder Paralichthys olivaceus to define the simplest, most rapid, and most effective method of ploidy analysis in aquaculture farms. Female triploidy of the flounder King-Nupchi strain was induced by cold shock (3 min post-fertilization at 2-4℃ for 45 min). Triploid induction was confirmed by erythrocyte measurement (nuclear volume, 29.15±2.10 μm³); flow cytometry (2.14±0.03 pg/cell); chromosome count (3N=72); Ag-NOR banding; and silver staining. Silver staining of finned cells obtained using a solid tissue technique was the most effective method of ploidy verification.

• 한국수산과학회지
• /
• 제47권6호
• /
• pp.707-712
• /
• 2014

The effects of transport stocking density (500, 600 and 700 kg of olive flounder Paralichthys olivaceus/per water tank) on the physicochemical characteristics of muscle were investigated. Transport of the animals took, on average, 10 days from Busan, Korea, to Long Beach, CA, USA. There was no significant difference in the breaking strength of muscle among the three groups. There was also no significant difference in the ATP, lactate, or glycogen content of muscle from the 500 and 600 kg/tank stocking density groups at the beginning and after transport. However, the ATP content decreased sharply while the lactate content increased in muscle from fish transported at a density of 700 kg/tank at the beginning of transport, and the ATP and lactate contents in this group were stable after transport.