• Applied Biological Chemistry
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• 제38권6호
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• pp.549-553
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• 1995

갈화(Puerariae flos)추출물이 rat의 혈액, 뇌 및 간의 ethanol농도를 유의차있게 감소시켰다. 혈중 ethanol의 감소 효과는 ethanol을 투여한지 1시간 경과한 때에 대조군과 가장 많은 차이를 보였으며 열수 추출물이 80% ethanol 추출물보다 혈중 ethanol 농도를 더 크게 감소시킴이 관찰되었다. Ethanol 투여 10분 전에 갈화추출물 투여가 1시간 전 혹은 10분 후에 투여한 군에 비해서 ethanol 농도 저하에 더 효과적이었으며, 간과 뇌 조직에서도 ethanol의 농도가 저하되는 현상이 관찰되었다. 그러나 이 때 acetaldehyde는 검출되지 않았다. 혈중 ethanol 농도를 가장 효율적으로 감소시키는 갈화 추출물의 적정량은 55.7 mg/kg body weight이었다. 이번 실험을 위해 새로이 시도된 dichloromethane에 의한 시료 추출과 GC를 이용한 분석법은 간단, 신속 하면서도 좋은 분석결과를 보였다.

• 대한한의학방제학회지
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• 제10권1호
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• pp.169-180
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• 2002

This study was performed to investigate the effects of Radix Puerariae (RP), Flos Puerariae (FP) and Caules in Liquamen Phyllostachyos+Radix Puerariae water extracts on the alcohol dehydrogenase (ADH). transaminase (GOT, GPT) activities, and two hepatic antioxidant enzyme (SOD, catalase) activities in acute ethanol administered mice, and we have investigated the morphological changes that occur in hepatocytes of the experimental mice. The activities of ADH decreased compared with control group in the A1(66%), C1(57%), C2(54%) groups. The transaminase activites increased in the control groups compared with experimental groups. Ethanol treatment group without the RP or FP administration significantly lowered the activities of hepatic SOD and catalase, whereas MnSOD increased in the A1(27%) and B2(43%)groups, CuZnSOD increased in the B2(25%) and C2 groups. The catalase activites were increased in the A1(270%), A2(478%), B2(487%) and B1(770%) compared with control group, A lot of PAS-positive granules were observed in the A1, A2, C1 and C2 groups compared with the other groups. These results suggested that RP, FP and RP+Bamboo extracts administration may be prevent from liver damage in the alcohol treatment mice.

• Archives of Pharmacal Research
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• 제26권3호
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• pp.210-213
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• 2003

Puerariae Flos is a traditional herbal medicine that has long been used as a treatment for colds, diabetes, and hangovers. The herbal medicine contains a wide variety of isoflavones such as kakkalide, tectoridin, and tectorigenin. This study demonstrates that the substances undergo a certain degree of change depending on the storage period by the method of HPLC and $^{13}C-NMR$, and that the HPLC analysis can be used to determine the freshness of Puerariae Flos.

• 생약학회지
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• 제29권3호
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• pp.193-197
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• 1998

Two isoflavonoids were isolated from the flowers of Pueraria lobata (Flos puerariae) guided initially fractionation based on brine shrimp lethality assay. The structures were identified as 4',5,7-trihydroxy-6-methoxyisoflavone (tectorigenin) and 4',7-dihydroxy-6-methoxyisoflavone (glycitein), respectively, on the basis of their spectroscopic and physicochemical evidences.

• Journal of Acupuncture Research
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• 제18권6호
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• pp.206-214
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• 2001

The purpose of this study was to determine the effects of Puerariae flos herb-acupuncture on hippocampal neural cell proliferation. Sprague-Dawley rats were randomly assigned into 4 groups; control group, control with herb-acupuncture group, alcohol group, alcohol with herb-acupuncture group group. Control groups were received with NaCl, while alcohol intoxication groups were injected intraperitoneally with alcohol (2 g/㎏) twice per day for 3 days. Herb-acupuncture groups were injected on Zhongwan (CV 12) for 5 consecutive days. Bromo-deoxyuridine (BrdU) was injected into all animal per day for 5 days. For the detection of BrdU-positive cells in dentate gyrus of hippocampus, immunohistochemistry was performed. In alcohol group, a significant decrease in BrdU-positive cells was observed compared to control group. In alcohol with herb-acupuncture group, BruU-positive cells increased significantly compared to alcohol group. In conclusion, the present results revealed that new cell proliferation is enhanced in the dentate gyros of young Sprague-Dawley rats through Puerariae flos herb-acpuncuture in an acute alcoholic intoxication condition.

• 대한본초학회지
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• 제33권5호
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• pp.1-8
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• 2018

Objectives : The aim of this study is to investigate the preventive effect of Puerariae Flos ethanol extract (PE) on methionin and choline deficient (MCD)-diet-induced Nonalcoholic fatty liver disease (NAFLD) in C57BL/6J mice. Methods : In the in vivo experiments, C57BL/6J mice were divided into 4 groups; Normal group, Control group, MCD+PE 100 group, and MCD+PE 300 group. After 4 weeks, body weight, liver weight, biochemical parameters for liver function test, histological changes, reverse transcription polymerase chain reaction (RT-PCR), and Western blot were assessed. Results : Mice lost body weight with the MCD-diet and the MCD+PE 100 group and MCD+PE 300 groups lost less than the control group, though showed no statistical significance. Liver weights were decreased by the MCD diet, but MCD+PE 300 groups were increased significantly. In the liver function test, all the values were decreased with the MCD-diet, MCD+PE 100 group and MCD+PE 300 groups were increased significance. In histological findings of the livers, MCD-diet induced severe fatty accumulation in the livers, but this fatty change was reduced in the MCD+PE 100 group and MCD+PE 300 groups was inhibited respectively. In lipid accumulation factors (such as SREBP-1c, $C/EBP{\alpha}$, PPAR-${\gamma}$), MCD+PE 100 group and MCD+PE 300 groups showed inhibitory effect on liver lipogenesis by reducing associated gene expressions caused by MCD diet. Conclusions : We were able to know that Puerariae Flos ethanol extract (PE) shown hepatoprotective effects via a decrease on the hepatic lipogenesis factors in the experimental NAFLD Models.

• 한국식품영양과학회지
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• 제38권7호
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• pp.846-851
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• 2009

갈화 추출물을 농축하여 동결 건조해서 실험동물인 SD계 수컷 흰쥐의 실험식이에 0.5% 수준으로 첨가하여 4주간 사육하면서 체중은 7일 간격으로 섭취는 2일 간격으로 오전9시에 측정하였으며, 활성산소 및 항산화활성 측정은 2주간격으로 측정하였다. 또한 실험이 종료된 후 실험동물을 단두 도살하여 혈청 중 총콜레스테롤, HDL-콜레스테롤, 중성지방, 혈당, 인지질을 측정하여 지질 함량에 미치는 영향을 측정하였다. 갈화 추출물을 SD계 수컷 흰쥐에 갈화 추출물이 함유된 식이를 급여 후 식이섭취량, 체중증가량을 측정한 결과 갈화 추출물을 섭취한 rat이 대조군에 비해 모두 증가하였지만 군간 유의적 차이를 보이지 않았다. 갈화 추출물 급여 후 적출된 실험동물의 장기에서 대조군과 실험군의 무게 차이는 보이지 않았다. 사육 기간 동안 2주 간격으로 측정한 혈액 중 항산화와 활성산소에서는, 항산화에서는 4주째 갈화가 대조군에 비해 유의적으로 수치가 높게 나왔으며, 활성산소는 유의적인 차이를 보이지 않았다. 실험식이 종료 후 실험동물에서 적출한 장기의 무게를 측정한 결과 심장, 신장, 장, 간 모두 차이를 보이지 않았다. 또한, 혈청중의 총콜레스테롤 함량 측정 결과 갈화 추출물 급여군이 대조군에 비해 낮은 함량을 나타내었고, HDL-콜레스테롤 함량에서는 대조군에 비해 높은 함량을 나타내었지만 유의적으로 증가하지는 않았다. HDL-cholesterol/total cholesterol의 비율에서도 갈화 추출물 급여군이 유의적으로 증가하였다. LDL-cholesterol 함량은 갈화 추출물 급여군이 대조군에 비해 낮은 함량을 나타내었으나 유의적인 차이는 아니었다. 중성지방 함량은 갈화 추출물 급여군이 대조군에 비해 낮은 수치를 보였으며, 혈당 함량과 인지질 함량은 대조군에 비해 높은 수치였으나 유의적인 차이가 없었다. 이상에서와 같이 갈화 추출물 급여한 흰쥐를 이용한 동물실험에서 항산화능 및 혈청의 지질대사 개선 효과를 확인할 수 있었다. 이는 천연물의 조추출물을 이용한 기반연구로서 향후 갈화의 항산화 효과와 지질개선에 대한 분자생물학적 조직병리학적 후속연구가 필요하다고 사료되며, 이 연구의 결과를 바탕으로 갈화의 항산화식품이나 지질 개선 기능성식품 소재로의 활용이 기대된다.

• 대한한방내과학회지
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• 제25권1호
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• pp.106-118
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• 2004

Antigenotoxicity test (SOS chromotest), antimutagenecity test (Ames test), and antioxidant test (NBT method and xanthine-xanthine oxidase method) were carried out using water-soluble and methanolic extracts from Puerariae Flos. Against the mutagens MNNG and NQO, antigenotoxic activity of methanolic extracts were much more effective than that of water-soluble ones. When the methanolic extract was added to the certain concentration $(100{\mu}{\ell}/tube)$, antigenotoxic acivity against the mutagen MNNG was enhanced. Contrary to the water-soluble extract, the methanolic extract showed high antigenotoxicity against the mutagen NQO with increment of the extract. Against the mutagen MNNG with Ames test, antimutagenic activity of the methanolic extract at $300{\mu}{\ell}/tube$ was 96% as an inhibition ratio of revertant forming CFU/plate. The antioxidant activity of water-soluble extract was comparatively higher than that of the methanolic one.

• 대한한의학회지
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• 제21권3호
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• pp.186-198
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• 2000

Objectives : This experiment was conducted to verify the effects of some oriental herbs(Alny Cortex et Ramulus, Artemisiae Capillaris Herba, Aurantii Nobilis Pericarpium, Giseng Radix, Hoveniae Semen, Puerariae Flos, Puerariae Radix, and Xanthii Fructus) which have been used in the treatment of alcoholic diseases, on alcoholic metabolism, and on alcoholic liver damage. Methods : The effects of the herbs on the activities of alcohol dehydrogenase(ADH), aldehyde dehydrogenase(ALDH) were evaluated and their protective effects of liver function and cells from alcoholic damage were analysed. For the evaluation of the protective effects, the levels of glucose, triglyceride, BUN, AST, and ALT in serum of rats were measured. Results and Conclusions : It is concluded that Puerariae Radix interferes with the ADH activity directly, thereby reducing the toxicity of alcohol, resulting in enhancing alcohol-tolerance and protecting liver functions. Also Artemisiae Capillaris Herba interferes both ADH and ALDH activities. Isolation of the biologically active compounds from Puerariae Radix and its detailed characterization are matters for future research.

• Advances in Traditional Medicine
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• 제7권3호
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• pp.229-234
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• 2007

In macrophages, nitric oxide (NO) is released as an inflammatory mediator and has been proposed to be an important modulator of many pathophysiological conditions including inflammation. In this study, we have examined the inhibition effects of NO production by 85% methanol extract of the flower of Pueraria thunbergiana (PF) in mouse macrophages. Extract of PF (1, 10, 100 ${\mu}g/ml$) inhibited NO production, inducible NO synthase and cyclooxygenase-2 expression in interferon-g and lipopolysaccharide-stimulated mouse peritoneal macrophages and it had no cytotoxicity. These data suggest that 85% methanol extract of PF might be useful in controlling macrophages mediated inflammatory disease.