• Korean Journal of Plant Resources
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• v.35 no.5
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• pp.652-658
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• 2022

This study was conducted to induce somatic embryogenic callus (SEC) derived from petals in rose. The petal explants of 3 cultivars ('Ice Wing', 'Orange Eye' and 'Pink Beauty') with different flower colors were placed on three types media (MS, SH and WPM) supplemented with 11 mg/L 2,4-D, respectively, and then cultured in the dark for 47 days. Calluses were formed at explants of all three cultivars. Also, 'Ice Wing', which were cultured in the SH as the basal medium, showed the highest callus formation rate. However, somatic embryos were generated from only petal-derived callus of 'Ice Wing', which were induced on the WPM as the basal medium, transferred it to SH basal medium supplemented with 3 mg/L 2,4-D, and 300 mg/L L-proline, and cultured for 5 weeks. The SEC has been proliferated every four weeks at the subculture interval. In addition, as a results of making a comparison of expression of RhSERK3 and RhSERK4, which is used as signal for generation of somatic embryo from callus in rose, between the SEC and petal-derived callus from 'Ice Wing' by RT-qPCR, the former showed 10 times higher RhSERK3 expression and 700 times higher RhSERK4 expression than the latter.

• Journal of Plant Biotechnology
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• v.36 no.4
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• pp.366-372
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• 2009

This study was conducted to develop new lines expressing the characteristic of early flowering by introducing MdMADS2 gene in chrysanthemum (Dendranthema grandiflorum (Ramat.) Kitamura) ‘Zinba'. Transformation of chrysanthemum was conducted by Agrobacterium tumefaciens LBA4404 harboring the binary vector containing MdMADS2 controlled by double CaMV 35S promoters. Ninety three shoots were regenerated from 1,463 leaf segment explants cultured on the first selection medium (MS basal salts + 1.0 mg/L BA + 0.5 mg/L IAA + 10 mg/L kanamycin + 400 mg/L cefotaxime, pH 5.8) after co-cultivation, and 20 out of the 93 shoots rooted on the second selection medium containing 20 mg/L kanamycin and 400 mg/L cefotaxime. Many escapes (98.6%) were removed on the selection stage for rooting. Nineteen lines were confirmed as transgenic plant with transgene by PCR analysis. Six transgenic plants flowered 2-11 days earlier than non-transgenic plant without big change of phenotype, and especially, 3 (Mo-7, Mo-11, Mo-17) out of 6 transgenic lines showed a significant reduction in days to flowering compared to non-transgenic plant. Introduction and expression of MdMADS2 gene in them were confirmed by Southern and real-time PCR analyses, respectively.

• Horticultural Science & Technology
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• v.32 no.3
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• pp.415-419
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• 2014

A Cymbidium variety 'Snow Bell' (C. kanran ${\times}$ C. Jack Frost) was developed at the National Institute of Horticultural & Herbal Science, Rural Development Administration in 2011. A cross between C. kanran with small-sized greenish colored flowers and C. Jack Frost with bright pink colored flowers, was conducted in 1999. Based on flower color, leaf shape, flower stalk, and vigor one line was selected. Evaluation and selection trials were conducted from 2006 to 2010. 'Snow Bell' has a light pink sepal (RHS, R56D), red lip (R56A) and a leaf length of 47.5 cm. The average number of flowers on each peduncle is 13.8 and the flower diameter is 6.2 cm. The characteristics of petals and sepals show some incurve and some spreading. 'Snow Bell' has a vigorous growth and a round flower. It has an adequate peduncle height of 50.7 cm compared with leaf length of 47.5 cm, and start blooming in December. In electronic nose analysis, the degree of fragrance between 'Snow Bell' and the C. kanran was similar and both of them showed strong scents, although they had different fragrance patterns.

• Journal of People, Plants, and Environment
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• v.22 no.1
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• pp.91-98
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• 2019

This study aimed to provide basic data on current trends in floriculture through a survey. The exhibitors' purposes and items displayed at the Tokyo International Flowers & Plants Expo in 2017 were surveyed. The survey was conducted among 238 exhibitors during the exhibition period from October 11 to 13, 2017. As a result, participants came from 14 countries including Belgium, China, Colombia, Japan, Korea and France. The purposes of the majority of the surveyed exhibitors were to display cultivars (86 exhibitors, 36.3%), followed by to display merchandise, subsidiary materials and cultivation techniques. Among cultivars, pot plants accounted for the majority of the entire cultivars (54 exhibitors, 55.1%), followed by cut flowers (35 exhibitors, 35.7%), sapling, and seeds. The most of the displayed pot plants were ornamental plants (27 exhibitors, 43.5%), followed by garden trees, ground cover plants, bedding plants and succulent plants. The most of the displayed cut flowers were rose (8 exhibitors, 21.1%), followed by lisianthus, chrysanthemum and dahila. In terms of cultivation techniques, those using moss were displayed by 7 exhibitors (50%) and green-wall techniques were displayed by 6 exhibitors (42.9%). In terms of merchandise, processed products were displayed by 21 exhibitors (41.2%), followed by floral products. Therefore, the IFEX can be used as a place to understand current trends in the floriculture market.

• Horticultural Science & Technology
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• v.30 no.6
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• pp.718-724
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• 2012

Sugars play important roles in petal senescence of cut flowers. In the Expt. 1 of this study, the effects of different concentrations of glucose (60, 90, and $120g{\cdot}L^{-1}$) and sucrose (30, 60, and $90g{\cdot}L^{-1}$) application on the vase life, rate of flower diameter increase, rate of flower weight increase and ethylene production of cut tree peony (Paeonia suffruticosa 'Luoyang Hong') were evaluated. At the earlier stage, treatments of different concentrations of glucose and sucrose all retarded the process of flower opening and inhibited the increase of flower diameter and weight, while senescence of flowers fed with different concentrations of glucose was delayed at later stage. Flowers treated with $90g{\cdot}L^{-1}$ glucose displayed the longest vase life, which showed significant difference (P < 0.05) from those of flowers with the control and sucrose treatments. All treatments with glucose or sucrose not only retarded the decrease of flower diameter and weight, but also suppressed the ethylene production at the earlier stage and delayed the peak of ethylene evolution. In order to study the effect of exogenous sugar on the postharvest response of cut tree peony to ethylene, Expt. 2 was conducted. Cut flowers were treated with $90g{\cdot}L^{-1}$ glucose for 4 hours before (GE) or after (EG) exposed to $10{\mu}L{\cdot}L^{-1}$ ethylene for 4 hours. Generally, the opening process of flowers with GE and EG treatments was similar to that of the control, however GE treatment delayed flower senescence. Both GE and EG treatments improved flower diameter and weight, and GE treatment delayed the time of flower weight decrease. Besides, GE delayed climacteric ethylene evolution for 8 hours. All above suggest that exogenous sugars delay tree peony 'Luoyang Hong' cut flower senescence and extend flower vase life through their roles in the decrease of water loss and the suppression of sensitivity to ethylene and ethylene production.

• Horticultural Science & Technology
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• v.29 no.6
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• pp.651-654
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• 2011

Cymbidium 'Orange Bowl' (Lucky Rainbow 'Randevous' ${\times}$ 'Eastern Star') was developed from a cross between hybrids at the National Institute of Horticultural & Herbal Science, Rural Development Administration in 2006. A cross was made between the pink colored flower C. Lucky Rainbow 'Randevous' as maternal line and pure yellow colored flower, C. 'Eastern Star' as paternal line in 1995. The seed germination, cultivation, selection, and characteristic trials were conducted from 1996 to 2006. The line was named as Wongyo F1-18 and phenotype was characterized in 2006 as a new 'Orange Bowl'. The 'Orange Bowl' has having light yellow basal color (RHS, YO21D) and orange line (RHS, OR30B) on both of sepal and petal with red lip (RHS, OR30B). 'Orange Bowl' has about 10.9 flowers per flower stalk and flower size of 7.4 cm. General appearance of the petals and sepals is slightly incurved shape. The plant size is intermediate having erect peduncle. Blooming is started from the late of January (mid winter) under optimal culture condition. Leaf attitude and twisting is half- erect and very weak respectively. This hybrid has attractive floral arrangement, long flower stalk (71.8 cm) and vigorous growth. We expect that 'Orange bowl' has a great potential for exporting to Chinese market.

• Journal of Bio-Environment Control
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• v.19 no.4
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• pp.257-265
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• 2010

The objective of study was carried out to investigate the proper nutrient nitrogen concentration and irrigation period for increasing plant growth and flowering in Ardisia pusilla. Nutrient nitrogen concentrations were 120, 150, 180 and $210\;mg{\cdot}L^{-1}$ and they were based on the Sonneveld solution. Irrigation periods were divided into ED (except dormancy) and TG (total growth) according to plant age. The results of plant age and irrigation period, growth of 1 year-old plant was promoted by nitrogen concentration above $150\;mg{\cdot}L^{-1}$ regardless of irrigation period. And plant growth values of 2 years-old in TG treatments were higher than ED treatments, especially TG-180 treatment was best of all. The contents of total nitrogen of leaves after flowering were increased with nutrient nitrogen strength. And the contents of potassium, calcium, magnesium and phosphate slightly were decreased or were no significant differences. Plant growth and flowering decreased when nitrogen concentration was over $210\;mg{\cdot}L^{-1}$. Therefore, TG-150 and TG-180 were supposed to be appropriate treatment for plant growth and flowering of 1year-old plant and 2 years old plant, respectively.

• Korean Journal of Breeding Science
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• v.43 no.6
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• pp.591-594
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• 2011

A new poinsettia(Euphorbia pulcherrima. Willd. ex Klotzch) cultivar 'Red Stone' was bred by the National Institute of Horticultural and Herbal Science (NIHHS) in 2009. In 2006, a cross was made between 'Freedom Rose', a cultivar with vigorous growth and deep pink bracts, and 'Enduring Pink', a cultivar with pink bracts early colored. 'Red Stone' was finally selected in 2009 after the investigation of the growth and flowering characteristics from 2007 to 2009. 'Red Stone' has red elliptic bracts and strong rugosities between bract veins. Leaf blade is ovate and very dark green. Stem color is reddish and petiole length is medium but relatively longer than that of 'Pepride Red', control cultivar. 'Red Stone' develops as many as 3.8 branches and which is more than double of 'Pepride Red'. Its bracts and transitional leaves are fully colored at 7.5 weeks after short-day commencement. Plant variety protection right of 'Red Stone' was registered in April 2011, and its grant number is 3487.

• FLOWER RESEARCH JOURNAL
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• v.18 no.3
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• pp.216-219
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• 2010

An Asiatic lily cultivar 'Yeri' was developed in 2005 at National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA), Korea. The cross was made in 1993 between Asiatic lily 'Geneve', a light pink colored cultivar, and 'Montreux', deep purple colored cultivar. The first selection was done and was tentatively named as 'A95-68' in 1995. After in vitro multiplication and bulbing production, growth and flowering characteristic tests were conducted from 1996 to 2003. The evaluation of characteristics was performed and named as 'Wongyo C1-19' in 2005 that was registered as 'Yeri' to the registration office of Korea Seed & Variety Service. 'Yeri' flowered at the first of July and grew average 34.6 cm stem in length. Flowers bloomed facing upward, unspotted in petals and deep purple (RHS, RP58A). The size of flower was 13.3 cm. Mean petal length and width was 7.7 cm and 2.7 cm, respectively. Leaves were 5.1 cm long and 0.5 cm wide, respectively. The weight and size of bulb were 9.6 g and 11.7 cm, respectively. Year-round flowering can be done by storing the bulb under $-1.5^{\circ}C$ conditions. For forced cultivation, it was necessary to add calcium to the fertilizer or remove side scales to prevent leaf scorch. It was needed to control Botrytis disease in wet season.

• FLOWER RESEARCH JOURNAL
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• v.18 no.4
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• pp.278-283
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• 2010

This study was conducted to investigate the involvement of flower abnormality and S-adenosyl-L-homocysteine hydrolase (SAHH), which is one of the key enzyme in the maintenance of methylation. Plants exposed to high temperature (HT) and long day (LD) condition from 14-27days after short day (SD) produced abnormal flower, having numbers of ray florets. Numbers of ray florets were increased more than 2 folds by HT of $35/20^{\circ}C$ and LD of 14 hour comparing those of $25/20^{\circ}C$ (12 h/12 h). Full-length cDNA clone of S-adenosyl-L-homocysteine hydrolase (DgSAHH) in spray chrysanthemum 'Lerbin' contained an 1455 bp open reading frame coding for 485 amino acids. It showed highly conserved coding sequences among the different plant species with over 90% homology. DgSAHH expression was decreased in abnormal flower inducing treatment of HT and LD, while DgSAHH transcripts accumulated in flower bud of non abnormality inducing condition. This result implicate that DgSAHH expression is affected by temperature and photoperiod during flower development and suppression of DgSAHH is a one of the cause of abnormal flower under HT and LD condition.