FLOWER RESEARCH JOURNAL (화훼연구)

The Korean Society for Floricultural Science (한국화훼학회)
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S-adenosyl-L-homocysteine hydrolase gene is down-regulated in abnormal flower inducing environment in chyrsanthemum

국화 기형화 발생과 S-adenosyl-L-homocysteine hydrolase 유전자 발현

  • Huh, Yeun Joo (Floriculture Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Park, Sang Kun (Floriculture Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Lim, Jin Hee (Floriculture Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Choi, Seong Youl (Floriculture Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • Lee, Young Ryan (Floriculture Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
  • 허은주 (농촌진흥청 국립원예특작과학원 화훼과) ;
  • 박상근 (농촌진흥청 국립원예특작과학원 화훼과) ;
  • 임진희 (농촌진흥청 국립원예특작과학원 화훼과) ;
  • 최성열 (농촌진흥청 국립원예특작과학원 화훼과) ;
  • 이영란 (농촌진흥청 국립원예특작과학원 화훼과)
  • Received : 2010.08.23
  • Accepted : 2010.10.25
  • Published : 2010.12.31
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Abstract

This study was conducted to investigate the involvement of flower abnormality and S-adenosyl-L-homocysteine hydrolase (SAHH), which is one of the key enzyme in the maintenance of methylation. Plants exposed to high temperature (HT) and long day (LD) condition from 14-27days after short day (SD) produced abnormal flower, having numbers of ray florets. Numbers of ray florets were increased more than 2 folds by HT of $35/20^{\circ}C$ and LD of 14 hour comparing those of $25/20^{\circ}C$ (12 h/12 h). Full-length cDNA clone of S-adenosyl-L-homocysteine hydrolase (DgSAHH) in spray chrysanthemum 'Lerbin' contained an 1455 bp open reading frame coding for 485 amino acids. It showed highly conserved coding sequences among the different plant species with over 90% homology. DgSAHH expression was decreased in abnormal flower inducing treatment of HT and LD, while DgSAHH transcripts accumulated in flower bud of non abnormality inducing condition. This result implicate that DgSAHH expression is affected by temperature and photoperiod during flower development and suppression of DgSAHH is a one of the cause of abnormal flower under HT and LD condition.

본 시험은 국화에서의 기형화 발생과 DNA 메틸레이션에 관여하는 S-adenosyl-L-homocysteine hydrolase (SAHH) 유전자 발현과의 관련성을 검토하기 위해 수행하였다. 스프레이 국화 'Lerbin'은 단일후 14일에서 27일 사이에 고온과 장일 조건에서 기형화가 발생되어, $35/20^{\circ}C$의 고온과 14시간의 장일 처리 할 경우 $25/20^{\circ}C$(12 h/12 h)에 비해 설상화가 2배 이상 증가하는 양상을 보였다. 스프레이 국화 'Lerbin'에서 분리한 전장 cDNA (DgSAHH)는 크기가 1455 bp로 다른 작물과 90%이상의 높은 상동성을 나타내었다. 국화의 DgSAHH 유전자 발현은 기형화 발생을 유도하는 고온과 장일 조건에서 크게 감소하였다. 이러한 결과를 통해 국화의 화아 발달에 있어서 DgSAHH 유전자 발현은 온도와 일장의 영향을 받으며, 억제된 이 유전자의 발현이 기형화 발생에 관여할 수 있을 것으로 판단된다.

Keywords

References

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